Duox2 (NM_024141) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Thox2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR201755 representing NM_024141 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTCCAACAAGTCTCAAGACATTGGTGCTCCTGGGCGCTCTGTTGACTGGACCCCTGGGCCCAGCAG GTGGCCAGGATGCACCCTCACTGCCCCGGGAAGTGCAGCGCTACGACGGCTGGTTTAATAATCTCAAGTA CCACCAGCGCGGTGCAGCTGGCTCGCAGCTGCGTCGCCTAGTACCTGCTAATTACGCTGACGGTGTTTAT CAGGCTCTGCAGGAGCCGCTACTCCCCAACGCTCGCCTGCTAAGCGACGCTGTCAGTAAGGGCAAAGCAG GGCTGCCCTCGGCGCACAACCGCACAGTGCTTGGGCTCTTCTTTGGCTACCACGTGCTCTCAGACCTGGT GAGTGTGGAAACACCAGGCTGCCCCGCAGAGTTCCTCAACATTTACATCCCACGTGGGGACCCAGTGTTT GACCCTGACAAGCGCGGGAACGTGGTGCTGCCCTTTCAGAGGAGCCGCTGGGACCGCAGCACTGGACAGA GCCCTAGCAACCCCCGAGACCTGACCAACCAGGTGACTGGCTGGTTAGATGGCAGCGCCATCTATGGCTC CTCTCATTCCTGGAGCGACACTCTGAGGAGCTTCTCTGGAGGACAGCTGGCTTCTGGCCCTGACCCTGCT TTCCCGAGGAACTCCCAGAACTCTCTGCTCATGTGGATGGCACCAGACCCCGCCACGGGGCAGGGCGGGC CACAGGGGCTGTATGCCTTCGGGGCCCAGCGCGGGAACAGGGAGCCCTTTCTGCAGGCACTGGGCTTGTT GTGGTTTCGATACCACAACCTGTGTGCCAAGAGGCTGGCGCAGGAGCACCCACACTGGGGGGATGAGGAG CTGTTCCAGCATGCTCGCAAGAGGGTCATTGCCACTTACCAGAACATTGCTCTATACCAATGGTTGCCCA GCTTCCTGCAGAAAACACCCCCAGAGTATTCCGGGTACCGCCCTTTCATGGACCCCAGCATCTCCCCAGA GTTCGTGGCGGCCTCAGAGCAGTTCCTCTCTACCATGGTGCCCCCGGGGGTCTATATGAGAAACTCCAGC TGTCACTTCCGGGAATTCCCAAAGGAAGGTTCCAGCAGCTCTCCAGCTCTCAGGGTCTGCAACAACTACT GGATTCGGGAGAACCCCAGTCTGAAGACTGCTCAGGACGTGGACCAGCTGCTGCTGGGAATGGCTTCCCA GATTTCAGAGCTGGAGGACAGGATAGTGATTGAAGATCTGAGAGATTACTGGCCTGGCCCAGACAGATAC TCTCGCACAGACTACGTGGCTAGCAGTATCCAGAGTGGCCGAGATATGGGGCTGCCCAGTTATAGCCAGG CTCTGCAGGCCTTAGGACTGGAGCCTCCCAAGAATTGGAGTGCTCTCAACCCCAAAGTGGATCCCCAGGT GCTGGAGGCCACAGCTGCTCTGTACAACCAGGACCTGTCCCGGTTGGAACTATTCCTGGGAGGGCTCCTG GAGAGCCATGGGGACCCTGGACCTCTATTCAGCAACATCATTCTTGACCAGTTTGTAAGGCTCCGGGATG GTGATCGCTACTGGTTTGAGAACACTAGGAACGGGCTGTTCTCCAAGGAGGAGATTGCAGAAATCAGAAA CACCACCTTGCGGGATGTACTGGTAGCTGTCTCCAATGTGGACCCCAGTGCCTTGCAGCCCAATGTTTTC TTCTGGCAAGAAGGTGCACCCTGCCCACAGCCTCAGCAACTCACAACTGAAGGCTTGCCCCAGTGTGTGC CTGTTACTGTGATTGACTACTTTGAGGGCAGTGGTGCTGGCTATGGTGTCACCCTCCTGGCTGTCTGCTG CTTTCCAGTAGTGAGTCTGATTATTGCTTGGGTGGTGGCTCGTTTCCGGAACCGAGAACGCAAGATGCTA CTAAAGAAAGGCAAGGAGAGTCTAAAGAAACAGACAGCCAGTGATGGAGTACCAGCGATGGAGTGGCCAG GCCCCAAGGAGAGCAGCTACCCAGTCACTGTCCAGCTGCTTCCAGACAGGAGTCTGAAGGTCCTTGACAA AAGGCTCACTGTGCTCCGTACCATCCAACTGCAGCCCACACAGCAGGTCAACCTCATCCTGTCCAGCAGC CATGGACGCCGCACCCTGTTGCTCAAGATCCCCAAGGAGTATGACCTGGTGCTGATGTTTAACTCTGAAG AGGACCGGGATGCCTTCGTGCAGCTGTTGCAAGACCTCTGCGTCTGCTCCACTCCCGGCCTCCGCATAGC TGAGATGGATGAGAAGGAGCTACTGAGAAAGGCTGTGACCAAGCAGCAGCGGGCAGGCATCTTGGAGATC TTCTTCAGACAGCTTTTTGCTCAGGTGCTGGACATCAATCAGGCCGACGCAGGGACTCTGCCTCTGGACT CATCCCAGCAAGTACGAGAGGCTCTGACCTGTGAACTGAGCAGAGCGGAGTTTGCGGACTCCCTGGGCCT CAAGCCCCAGGATATGTTTGTGGAGTCCATGTTTTCTCTGGCCGACAAGGATGGCAATGGCTACATATCC TTCCGGGAGTTCCTGGACATCCTGGTGGTTTTCATGAAAGGTTCCCCACAGGACAAGTCTCGCCTAATGT TTACCATGTATGACCTGGATGGAAATGGCTTTCTCTCCAAGGAGGAATTCTTCACCATGATGCGGTCCTT CATCGAGATCTCCAACAACTGCCTGTCCAAGGACCAGCTGGCTGAGGTGGTGGAATCCATGTTCCGGGAG TCGGGCTTCCAGGACAAGGAGGAGCTGACCTGGGAGGACTTCCATTTCATGCTGCGCGACCACGACAGCG ATCTCCGATTCACACAGCTCTGTGTCAAAGGTGGGGCTGGAGGCACCGGGGACATCTTTAAGCAAAGCAA TGCCTGTCGAGTCTCATTCCTCACCCGGACTCCTGGGAACAGGGTAATGGCTCCCTCTCCCCGGCTGTAC ACGGAAGCACTGCAGGAGAAAATGCAGAGGGGCTTCCTAGCCCAGAAGCTCAAGCAGTTCAAGCGGTTTG TGGAAAACTACCGGCGCCACATCGTGTGTGTTACAATCTTCTCAGCCATCTGCGCAGGCCTGTTTGCAGA CCGTGCCTACTACTATGGCTTTGCCTCGCCACCCACGGACATTGAAGAAACCACCTATGTGGGCATCATC CTGTCCCGGGGCACAGCAGCCAGCATCTCCTTCATGTTCTCCTACATCCTGCTCACCATGTGCCGCAACC TCATCACCTTCCTGCGGGAGACCTTCCTCAACCGCTACATTCCCTTTGATGCCGCTGTGGACTTCCACCG CTGGATCGCCATGGCTGCGGTTGTCCTAGCTGTTGTGCACAGTCTTGGACATGCAGTCAACGTGTACATC TTCTCAGTCAGTCCCCTCAGCCTGATGACCTGCGTCTTCCCTAGTGTCTTTGTGAATGATGGGTCCAAGC TTCCCCCAAAGTACTACTGGTGGTTCTTCGAGACAGTTCCAGGTATGACAGGAGTACTCCTGCTCCTGGT CCTGGCTATCATGTATGTGTTTGCCTCCCACCACTTCCGCCGCCACAGTTTCCGGGGCTTCTGGTTGACC CACCACCTCTATGTTGTGCTTTATGCCCTGATCATCATCCATGGCAGCTATGCCCTCATCCAATTACCCA GCTTCCACATCTACTTCCTGGTCCCCGCCATTATCTATGTGGGGGACAAGCTGGTGAGCCTGAGCCGGAA GAAGGTGGAGATCAGTGTGGTGAAGGTTGAGCTGCTGCCTTCAGGGGTGACCTATCTGCAGTTCCAGCGG CCCAAGACTTTTGAGTACAAATCCGGGCAGTGGGTGCGGATCGCTTGCTTGAGTCTGGGAACCAATGAGT ACCACCCCTTCACGCTGACCTCCGCACCCCATGAGGACACACTCAGCCTGCACATCCGGGCTGTGGGACC TTGGACTACTCGCCTCAGGGAGATCTACTCACCTCCAGTGGGTGGCACCAGCGCCAGATACCCAAAGCTG TACCTCGATGGACCATTCGGAGAGGGCCACCAGGAATGGCACAAGTTTGAGGTGTCGGTGCTGGTAGGAG GGGGCATTGGGGTCACCCCCTTTGCCTCCATCCTCAAAGACCTGGTCTTCAAATCGTCCATGGGTACCCA GATGCTATGTAAGAAGATCTACTTCATCTGGGTGACAAGGACTCAGCGGCAGTTTGAGTGGCTGGCTGAC ATCATCCGGGAAGTGGAGGAGAATGGCAGCCGGGACCTGGTGTCTGTGCACATCTATATCACTCAGCTGG CTGAGAAGTTTGACCTCAGGACGACCATGCTGTACATCTGTGAGAGGCACTTCCAGAAGGTGCTGAACAG GAGTCTGTTCACGGGCCTGCGCTCTGTCACCCACTTTGGTCGCCCTCCCTTTGAGCTCTTCTTAGACTCT CTGCAGGAGGTTCATCCACAGGTACATAAGATTGGTGTGTTCAGCTGTGGTCCTCCAGGGATGACCAAGA ATGTGGAGAAGGCCTGTCAGCTCATCAACAGGCAGGACCGGGCCCACTTTGTGCATCATTATGAGAACTT C ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR201755 representing NM_024141 Red=Cloning site Green=Tags(s) MLPTSLKTLVLLGALLTGPLGPAGGQDAPSLPREVQRYDGWFNNLKYHQRGAAGSQLRRLVPANYADGVY QALQEPLLPNARLLSDAVSKGKAGLPSAHNRTVLGLFFGYHVLSDLVSVETPGCPAEFLNIYIPRGDPVF DPDKRGNVVLPFQRSRWDRSTGQSPSNPRDLTNQVTGWLDGSAIYGSSHSWSDTLRSFSGGQLASGPDPA FPRNSQNSLLMWMAPDPATGQGGPQGLYAFGAQRGNREPFLQALGLLWFRYHNLCAKRLAQEHPHWGDEE LFQHARKRVIATYQNIALYQWLPSFLQKTPPEYSGYRPFMDPSISPEFVAASEQFLSTMVPPGVYMRNSS CHFREFPKEGSSSSPALRVCNNYWIRENPSLKTAQDVDQLLLGMASQISELEDRIVIEDLRDYWPGPDRY SRTDYVASSIQSGRDMGLPSYSQALQALGLEPPKNWSALNPKVDPQVLEATAALYNQDLSRLELFLGGLL ESHGDPGPLFSNIILDQFVRLRDGDRYWFENTRNGLFSKEEIAEIRNTTLRDVLVAVSNVDPSALQPNVF FWQEGAPCPQPQQLTTEGLPQCVPVTVIDYFEGSGAGYGVTLLAVCCFPVVSLIIAWVVARFRNRERKML LKKGKESLKKQTASDGVPAMEWPGPKESSYPVTVQLLPDRSLKVLDKRLTVLRTIQLQPTQQVNLILSSS HGRRTLLLKIPKEYDLVLMFNSEEDRDAFVQLLQDLCVCSTPGLRIAEMDEKELLRKAVTKQQRAGILEI FFRQLFAQVLDINQADAGTLPLDSSQQVREALTCELSRAEFADSLGLKPQDMFVESMFSLADKDGNGYIS FREFLDILVVFMKGSPQDKSRLMFTMYDLDGNGFLSKEEFFTMMRSFIEISNNCLSKDQLAEVVESMFRE SGFQDKEELTWEDFHFMLRDHDSDLRFTQLCVKGGAGGTGDIFKQSNACRVSFLTRTPGNRVMAPSPRLY TEALQEKMQRGFLAQKLKQFKRFVENYRRHIVCVTIFSAICAGLFADRAYYYGFASPPTDIEETTYVGII LSRGTAASISFMFSYILLTMCRNLITFLRETFLNRYIPFDAAVDFHRWIAMAAVVLAVVHSLGHAVNVYI FSVSPLSLMTCVFPSVFVNDGSKLPPKYYWWFFETVPGMTGVLLLLVLAIMYVFASHHFRRHSFRGFWLT HHLYVVLYALIIIHGSYALIQLPSFHIYFLVPAIIYVGDKLVSLSRKKVEISVVKVELLPSGVTYLQFQR PKTFEYKSGQWVRIACLSLGTNEYHPFTLTSAPHEDTLSLHIRAVGPWTTRLREIYSPPVGGTSARYPKL YLDGPFGEGHQEWHKFEVSVLVGGGIGVTPFASILKDLVFKSSMGTQMLCKKIYFIWVTRTQRQFEWLAD IIREVEENGSRDLVSVHIYITQLAEKFDLRTTMLYICERHFQKVLNRSLFTGLRSVTHFGRPPFELFLDS LQEVHPQVHKIGVFSCGPPGMTKNVEKACQLINRQDRAHFVHHYENF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_024141
ORF Size	4551 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_024141.1, NP_077055.1
RefSeq Size	5689 bp
RefSeq ORF	4554 bp
Locus ID	79107
UniProt ID	Q9ES45
MW	171.6 kDa
Gene Summary	a thyroid NADPH oxidase flavoprotein [RGD, Feb 2006]

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