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Babam2 (NM_199270) Rat Tagged ORF Clone
CAT＃: RR201508
- TrueORF®
Bre (Myc-DDK-tagged ORF) - Rat brain and reproductive organ-expressed protein (Bre), (10 ug)
"NM_199270" in other vectors (3)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR201508 representing NM_199270
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCCAGAAATTGCCTTGAACCGAATTTCTCCAATGCTCTCCCCATTCATATCGAGCGTGGTCCGAA ATGGGAAGGTGGGACTGGATGCTACCAACTGTTTGAGGATAACGGACTTGAAGTCTGGCTGTACATCCTT GACTCCTGGACCCAACTGTGACCGCTTCAAACTGCACATCCCGTACGCTGGAGAGACATTAAAGTGGGAC ATAATTTTCAATGCTCAGTACCCAGAGCTGCCTCCTGATTTCATCTTTGGAGAGGATGCCGAGTTCCTGC CAGACCCCTCTGCTCTGCACAACCTTGCCTCCTGGAACCCTTCGAACCCCGAGTGCCTGCTGCTTGTGGT GAAGGAGCTGGTGCAGCAGTACCACCAGTTTCAGTGCGGCCGCCTCCGTGAGAGCTCACGCCTCATGTTT GAGTACCAGACGCTCCTGGAAGAGCCTCAGTATGGAGAGAACATGGAAATTTATGCTGGGAAGAAAAACA ACTGGACTGGTGAATTTTCAGCTCGTTTTCTGTTGAAGTTACCAGTAGATTTCAGCAACATTCCCACGTA CCTTCTCAAGGATGTAAACGAAGATCCCGGAGAAGATGTGGCTCTCCTTTCTGTCAGCTTTGAGGATACT GAAGCCACCCAGGTGTACCCCAAGTTGTACTTGTCACCCCGAATTGAGCATGCCCTCGGGGGCTCCTCGG CTCTCCATATCCCTGCTTTTCCAGGAGGAGGATGTCTCATTGATTATGTGCCTCAAGTGTGCCACCTGCT CACCAACAAGGTGCAGTATGTGATTCAAGGCTATCACAAAAGAAGAGAGTACATCGCTGCTTTCCTCAGT CACTTTGGCACAGGTGTCGTGGAATATGATGCAGAAGGCTTCACAAAACTTACACTGCTGCTGATGTGGA AAGAGTTTTGTTTTCTTGTACACATTGACCTGCCTCTGTTTTTCCCTCGAGATCAGCCAACCCTCACGTT TCAGTCCGTCTACCACTTTACCAACAGTGGACAGCTTTACTCCCAAGCACAGAAAAATTACCCATACAGC CCCAGATGGGATGGAAATGAAATGGCCAAGAGAGCAAAGGCTTATTTCAAAACCTTTGTCCCTCAGTTCC AGGAGGCTGCATTTGCCAATGGAAAGCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR201508 representing NM_199270
Red=Cloning site Green=Tags(s) MSPEIALNRISPMLSPFISSVVRNGKVGLDATNCLRITDLKSGCTSLTPGPNCDRFKLHIPYAGETLKWD IIFNAQYPELPPDFIFGEDAEFLPDPSALHNLASWNPSNPECLLLVVKELVQQYHQFQCGRLRESSRLMF EYQTLLEEPQYGENMEIYAGKKNNWTGEFSARFLLKLPVDFSNIPTYLLKDVNEDPGEDVALLSVSFEDT EATQVYPKLYLSPRIEHALGGSSALHIPAFPGGGCLIDYVPQVCHLLTNKVQYVIQGYHKRREYIAAFLS HFGTGVVEYDAEGFTKLTLLLMWKEFCFLVHIDLPLFFPRDQPTLTFQSVYHFTNSGQLYSQAQKNYPYS PRWDGNEMAKRAKAYFKTFVPQFQEAAFANGKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_199270 |
| ORF Size | 1149 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_199270.1, NP_954891.1 |
| RefSeq Size | 1565 bp |
| RefSeq ORF | 1152 bp |
| Locus ID | 362704 |
| UniProt ID | Q6P7Q1 |
| MW | 43.6 kDa |
| Gene Summary | Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it acts as an adapter that bridges the interaction between BABAM1/NBA1 and the rest of the complex, thereby being required for the complex integrity and modulating the E3 ubiquitin ligase activity of the BRCA1-BARD1 heterodimer. Component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates. Within the BRISC complex, acts as an adapter that bridges the interaction between BABAM1/NBA1 and the rest of the complex, thereby being required for the complex integrity. The BRISC complex is required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1. The BRISC complex plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1; deubiquitination increases IFNAR1 activity by enhancing its stability and cell surface expression. Down-regulates the response to bacterial lipopolysaccharide (LPS) via its role in IFNAR1 deubiquitination. May play a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. May also act as a death receptor-associated anti-apoptotic protein, which inhibits the mitochondrial apoptotic pathway. May regulate TNF-alpha signaling through its interactions with TNFRSF1A; however these effects may be indirect.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN201508 | Bre (untagged ORF) - Rat brain and reproductive organ-expressed protein (Bre), (10 ug) |
CNY 3,990.00 |
|
| RR201508L3 | Lenti ORF clone of Bre (Myc-DDK-tagged ORF) - Rat brain and reproductive organ-expressed protein (Bre), (10 ug) |
CNY 6,080.00 |
|
| RR201508L4 | Lenti ORF clone of Bre (mGFP-tagged ORF) - Rat brain and reproductive organ-expressed protein (Bre), (10 ug) |
CNY 6,650.00 |
