Ankrd1 (NM_013220) Rat Tagged ORF Clone
CAT#: RR201420
- TrueORF®
Ankrd1 (Myc-DDK-tagged ORF) - Rat ankyrin repeat domain 1 (cardiac muscle) (Ankrd1), (10 ug)
"NM_013220" in other vectors (3)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Alrp; Carp; Crap; MARP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR201420 representing NM_013220
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGTTTTTCGAGTAGAGGAGCTGGTAACGGGCAAAAAGAACAGCAATGGGTCCTCAGGGGAGTTCC TTCCTGGCGAGTTCAGAAATGGAGAGTATGAAGCTGCTGTTGCCTTGGAGAAACAAGAGGACTTGAAGAC ACTTCCAGCCAACAGTGTGAACCTGGGGGAAGAACAACGGAAAAGTGAGAAAGTTCGAGAGGCAGAGCTC AAAAAGAAAAAACTAGAACAAAGATCAAAGCTTGAAAACTTAGAAGACCTTGAAATAATTGTTCAACTGA AGAAAAGAAAAAAATACAAGAAAACTAAAGTTCCAGTTGTGAAGGAACCGGAGCCTGAAATTATTACTGA ACCTGTGGATGTGCCAAGGTTTCTGAAAGCTGCACTGGAGAACAAACTGCCAGTTGTAGAGAAATTCCTG TCAGACAAGAACAGCCCCGACGTCTGCGATGAGTATAAACGGACCGCTCTCCATAGAGCATGCTTAGAAG GACACTTGGCGATCGTGGAGAAGTTAATGGAGGCTGGAGCCCAGATTGAATTCCGAGATATGCTGGAATC CACAGCCATCCACTGGGCATGTCGTGGAGGAAACCTGGATGTCCTGAAACTGTTGCTAAACAAAGGTGCC AAAATCAGTGCCCGAGACAAGCTGCTCAGCACAGCGCTGCACGTGGCGGTGAGGACCGGTCACTACGAGT GTGCTGAGCACCTCATCGCCTGCGAGGCGGATCTCAATGCCAAGGACAGAGAAGGAGACACCCCTCTGCA TGATGCGGTGAGGTTGAATCGCTACAAGATGATCCGGCTCTTGATGACCTTCGGTGCGGACCTCAATGTC AAGAACTGTGCTGGGAAGATCCCTATGGATCTGGTGTTGCACTGGCAGAATGGAACCAAAGCGATATTCG ACAGCCTCAAGGAGAATGCCTACAAAAACTCGCGCATAGCTACGTTCACAGCCTCAAGGAGAATGCCTAC AAAAACTCGCGCATAGCTACGTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR201420 representing NM_013220
Red=Cloning site Green=Tags(s) MMVFRVEELVTGKKNSNGSSGEFLPGEFRNGEYEAAVALEKQEDLKTLPANSVNLGEEQRKSEKVREAEL KKKKLEQRSKLENLEDLEIIVQLKKRKKYKKTKVPVVKEPEPEIITEPVDVPRFLKAALENKLPVVEKFL SDKNSPDVCDEYKRTALHRACLEGHLAIVEKLMEAGAQIEFRDMLESTAIHWACRGGNLDVLKLLLNKGA KISARDKLLSTALHVAVRTGHYECAEHLIACEADLNAKDREGDTPLHDAVRLNRYKMIRLLMTFGADLNV KNCAGKIPMDLVLHWQNGTKAIFDSLKENAYKNSRIATFTASRRMPTKTRA*LR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_013220 |
ORF Size | 957 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq Size | 1749 bp |
RefSeq ORF | 960 bp |
Locus ID | 27064 |
UniProt ID | Q8R560 |
MW | 36.1 kDa |
Gene Summary | acts as a negative regulator of cardiac-specific gene expression [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN201420 | Ankrd1 (untagged ORF) - Rat ankyrin repeat domain 1 (cardiac muscle) (Ankrd1), (10 ug) |
CNY 3,990.00 |
|
RR201420L3 | Lenti ORF clone of Ankrd1 (Myc-DDK-tagged ORF) - Rat ankyrin repeat domain 1 (cardiac muscle) (Ankrd1), (10 ug) |
CNY 6,080.00 |
|
RR201420L4 | Lenti ORF clone of Ankrd1 (mGFP-tagged ORF) - Rat ankyrin repeat domain 1 (cardiac muscle) (Ankrd1), (10 ug) |
CNY 6,650.00 |