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Aurka (NM_153296) Rat Tagged ORF Clone
CAT＃: RR201216
- TrueORF®
Aurka (Myc-DDK-tagged ORF) - Rat aurora kinase A (Aurka), (10 ug)
"NM_153296" in other vectors (3)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ARK-1; Stk6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR201216 representing NM_153296
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAGATGTAAAGAAAACTGTGTCTCTAGGCCTGTCAAGTCCACTGTTCCCTTCGGTCCGAAACGAG TCTTGGTGACTGAGCAGATTCCTTCTCAGCACCCAGGATCAGCGAGCAGTGGCCAGGCCCAGCGGGTCCT GTGTCCCTCCAACTCCCAGCGTGTCCCTCCACAAGCACAGAAACCTGTCGCAGGTCAGAAGCCAGTGCTC AAGCAGTTGCCGGCTGCCAGCGGTCCTCGACCTGCTTCCCGGCTGAGTAACCCCCAGAAGAGTGAGCAGC CACAGCCTGCGGCCTCGGGAAATAATTCTGAAAAGGAACAGACATCCATCCAGAAAACTGAAGACTCAAA AAAAAGGCAGTGGACTTTGGAAGATTTTGACATCGGCCGCCCACTAGGAAAAGGGAAGTTTGGAAATGTC TACTTGGCGAGGGAGAAGCAAAGCAAGTTCATCCTGGCTCTGAAGGTGCTGTTTAAAGTACAGCTGGAGA AGGCAGGTGTGGAGCACCAGCTCCGGAGAGAAGTGGAGATCCAGTCCCACCTGCGGCACCCCAACATCCT CAGGCTGTATGGGTATTTCCATGACGCCACCAGAGTTTATTTAATTCTGGAATACGCGCCCCTTGGAACA GTATATAGAGAGCTTCAAAAACTCTCCAAGTTTGATGAGCAGAGAACAGCTACCTACATCACGGAGTTGG CGAATGCTTTGTCCTACTGCCACTCAAAGAGAGTCATCCACAGAGACATCAAGCCAGAGAACCTGCTGCT TGGCTCAAATGGGGAGTTGAAGATCGCAGACTTTGGGTGGTCTGTGCATGCTCCGTCTTCCAGGAGAACC ACGCTGTGTGGCACCCTGGACTACCTGCCCCCGGAGATGATTGAAGGTCGGATGCATGACGAGAAGGTGG ACCTCTGGAGCCTGGGGGTGCTCTGCTATGAGTTCCTAGTGGGGATGCCTCCTTTTGAGGCACACACTTA TCAGGAGACCTACAGAAGGATATCACGGGTTGAATTCACATTCCCTGACTTTGTGACGGAGGGAGCCAGG GACCTCATTTCAAGACTGTTAAAACACAACTCAAGTCAAAGGCTAACACTAGCGGAAGTCCTTGAGCACC CTTGGATCAAAGCTAATTCTTCAAAACCTCCGACTGGCCACAATAGCAAAGAGGCAACTAGCAAATCGTC T ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR201216 representing NM_153296
Red=Cloning site Green=Tags(s) MDRCKENCVSRPVKSTVPFGPKRVLVTEQIPSQHPGSASSGQAQRVLCPSNSQRVPPQAQKPVAGQKPVL KQLPAASGPRPASRLSNPQKSEQPQPAASGNNSEKEQTSIQKTEDSKKRQWTLEDFDIGRPLGKGKFGNV YLAREKQSKFILALKVLFKVQLEKAGVEHQLRREVEIQSHLRHPNILRLYGYFHDATRVYLILEYAPLGT VYRELQKLSKFDEQRTATYITELANALSYCHSKRVIHRDIKPENLLLGSNGELKIADFGWSVHAPSSRRT TLCGTLDYLPPEMIEGRMHDEKVDLWSLGVLCYEFLVGMPPFEAHTYQETYRRISRVEFTFPDFVTEGAR DLISRLLKHNSSQRLTLAEVLEHPWIKANSSKPPTGHNSKEATSKSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_153296 |
| ORF Size | 1191 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_153296.2, NP_695208.2 |
| RefSeq Size | 2384 bp |
| RefSeq ORF | 1194 bp |
| Locus ID | 261730 |
| MW | 44.9 kDa |
| Gene Summary | serine/threonine protein kinase which is regulated by the cell cycle; may be involved in microtubule formation and/or stabilization at the spindle pole during chomosome segregation [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN201216 | Aurka (untagged ORF) - Rat aurora kinase A (Aurka), (10 ug) |
CNY 3,990.00 |
|
| RR201216L3 | Lenti ORF clone of Aurka (Myc-DDK-tagged ORF) - Rat aurora kinase A (Aurka), (10 ug) |
CNY 6,080.00 |
|
| RR201216L4 | Lenti ORF clone of Aurka (mGFP-tagged ORF) - Rat aurora kinase A (Aurka), (10 ug) |
CNY 6,650.00 |
