Cebpd (NM_013154) Rat Tagged ORF Clone

CAT#: RR201147

  • TrueORF®

Cebpd (Myc-DDK-tagged ORF) - Rat CCAAT/enhancer binding protein (C/EBP), delta (Cebpd), (10 ug)



  "NM_013154" in other vectors (3)

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CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit polyclonal Rat Cebpd Antibody (Center)
    • 400 ul

CNY 6,314.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Synonyms C/EBPd; CELF
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR201147 representing NM_013154
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCGCCGCTCTTTTCAGCCTAGACAGCCCAGCACGCGGCGCACCCTGGCCCACAGAGCCCGCGGCCT
TCTACGAGCCAGGCAGGGTGGGCAAGCCAGGACGAGGGCCGGAGCCTGGGGATCTGGGGGAGCCGGGCTC
CACGACCCCTGCCATGTATGACGACGAGAGCGCCATCGACTTCAGCGCCTACATTGATTCCATGGCTGCC
GTGCCCACCCTAGAGTTGTGCCACGACGAGATCTTCGCCGACCTCTTCAACAGCAATCACAAAGCGGCCG
GCGCGGGCAGCCTGGAGCTGCTGCAGGGCGGCCCTACGCGACCCCCGGGTGTGGGGTCAATCGCCAGGGG
CCCGCTGAAGCGCGAACCCGACTGGGGCGACGGCGACGCGCCGGGCTCCCTGCTGCCGGCGCAAGTGGCA
GTGTGCGCGCAGACAGTGGTGAGCTTGGCGGCCGCGGCACAGCCCACACCACCCACTTCGCCCGAGCCTC
CTCGAGGCAGCCCTGGACCGAGCCTTGCGCCTGGCCCCGTCCGAGAGAAGGGCGCCGGCAAGAGGGGTCC
GGACCGGGGCAGCCCTGAGTACCGGCAGCGACGCGAGCGCAACAACATCGCTGTGCGCAAGAGCCGGGAC
AAGGCCAAGCGCCGCAACCAGGAGATGCAGCAGAAGCTGGTGGAGCTGTCGGCCGAGAACGAGAAGCTGC
ATCAGCGTGTGGAGCAGCTCACCCGGGACCTGGCCAGCCTCCGGCAGTTCTTCAAAGAGCTGCCCAGCCC
GCCTTTCCTGCCGCCCACCGGCACCGACTGCCGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR201147 representing NM_013154
Red=Cloning site Green=Tags(s)

MSAALFSLDSPARGAPWPTEPAAFYEPGRVGKPGRGPEPGDLGEPGSTTPAMYDDESAIDFSAYIDSMAA
VPTLELCHDEIFADLFNSNHKAAGAGSLELLQGGPTRPPGVGSIARGPLKREPDWGDGDAPGSLLPAQVA
VCAQTVVSLAAAAQPTPPTSPEPPRGSPGPSLAPGPVREKGAGKRGPDRGSPEYRQRRERNNIAVRKSRD
KAKRRNQEMQQKLVELSAENEKLHQRVEQLTRDLASLRQFFKELPSPPFLPPTGTDCR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_013154
ORF Size 804 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_013154.2, NP_037286.1
RefSeq Size 1139 bp
RefSeq ORF 807 bp
Locus ID 25695
UniProt ID Q03484
MW 28.6 kDa
Gene Summary transcriptional activator; activity is stimulated by sepsis, keratinocyte growth factor, and may be involved in glucose-mediated regulation of iNOS [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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