Abcc1 (NM_022281) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Abcc1a; Avcc1a; Mrp; Mrp1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR200856 representing NM_022281 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTGAGCAGCTTCTGCAGCTCTGATGGCTCCGATCCGCTCTGGGATTGGAATGTCACATGGCACA CCAGCAACCCTGACTTTACCAAGTGCTTTCAGAATACGGTCCTCACATGGGTGCCTTGTTTCTACCTCTG GTCCTGTTTCCCCCTCTACTTCCTCTATCTCTCTCGACATGACCGGGGCTACATCCAGATGACACACCTC AACAAAGCCAAAACTGCCTTAGGATTCTTTCTGTGGATCATCTGCTGGGCAGACCTCTTCTACTCTTTCT GGGAAAGAAGTCAGGGAATGCTCCTAGCCCCGGTGCTACTGGTCAGCCCGACACTGCTAGGCATCACCAT GCTGCTCGCCACCTTTTTAATTCAGTTTGAGCGAAGGAAAGGAGTCCAGTCCTCAGGGATAATGCTTACT TTCTGGCTTGTAGCCCTACTCTGCGCCCTTGCCATCTTGAGATCTAAGATCATCTCTGCCTTAAAAAAGG ATGCTCAAGTGGACATGTTTCGAGATTCTGCATTCTATCTCTACTTCACCCTCGTGTTCATTCAGCTTGT GCTGTCCTGCTTCTCAGACAGCTCACCCTTGTTCTCTGAAACTGTCCGTGACCCGAATCCATGTCCAGAA TCGAGTGCCTCTTTTCTTTCCAGGATCACTTTTTGGTGGATTACAGGGATGATGGTGCAGGGCTACCGCC AGCCCCTGAAGAGCAGTGACCTCTGGTCATTGAATAAAGAGGACACGTCAGAAGAAGTGGTACCTGTGCT GGTGAATAACTGGAAGAAGGAATGTGTTAAGTCGAGGAAGCAGCCTGTACGGATTGTGTATGCCCCTCCC AAAGATCCCACCAAGCCTAAGGGAAGTTCTCAGTTGGATGTGAATGAGGAAGTGGAGGCACTGATTGTCA AGTCATCCCACAAGGACCGGGACCCCTCTCTGTTCAAGGTGTTGTACAAGACCTTTGGGCCCTACTTCCT CATGAGCTTCCTGTACAAGGCCCTTCATGACCTGATGATGTTTGCTGGCCCTGAGATCTTGGAATTGATT ATCAACTTCGTGAATGACAGGGAGGCCCCTGACTGGCAGGGCTACTTGTACACAGCACTGCTGTTTGTCA GTGCCTGTCTGCAGACACTGGCACTCCACCAGTACTTTCATATCTGCTTCGTCACCGGCATGCGCATCAA GACTGCTGTGGTGGGTGCTGTTTACCGCAAGGCTCTTGTGATCACCAATTCAGCTAGAAAATCGTCCACA GTTGGAGAGATTGTCAACCTCATGTCCGTGGATGCCCAGCGCTTCATGGACTTGGCCACGTATATTAACA TGATCTGGTCAGCCCCTCTGCAAGTCACCCTAGCCCTCTACTTCCTGTGGCTGAACCTGGGCCCTTCTGT GCTGGCTGGGGTGGCTGTTATGATCCTCATGGTGCCCTTCAATGCTGTGATGGCCATGAAGACCAAGACT TACCAGGTGGCACACATGAAGAGCAAAGACAACCGAATCAAGCTGATGAACGAGATCCTCAATGGGATCA AAGTACTCAAATTGTACGCCTGGGAGCTGGCTTTCCAGGACAAAGTTATGAACATCAGGCAGGAGGAGCT GAAGGTGCTGAAGAAATCCGCCTACCTGGCGGCTGTGGGCACATTCACATGGGTTTGCACACCTTTCCTG GTGGCTCTGTCAACCTTTGCTGTCTTTGTGACTGTGGACGAGAAGAACATCCTAGATGCAAAGAAAGCCT TTGTATCCCTAGCCCTGTTCAATATCTTGCGCTTCCCACTCAACATCCTACCCATGGTCATCAGCAGCAT TGTGCAGGCCAGCGTGTCCCTCAAGCGTCTCAGGATCTTTCTGTCTCACGAGGAGCTGGAGCCAGACAGC ATCGAGCGATGGTCGATCAAGGATGGTGGAGGGATGAATAGCATCACTGTGAAGAATGCAACCTTCACTT GGGCCAGGGATGAACCTCCCACACTGAATGGCATCACCTTCGCCATCCCTGATGGAGCCCTTGTGGCCGT GGTGGGCCAGGTAGGCTGTGGGAAGTCATCTCTGCTGTCAGCCCTGCTGGCTGAGATGGACAAAGTGGAG GGACATGTGACTCTCAAGGGCTCCGTGGCCTATGTGCCCCAGCAGGCCTGGATTCAGAATGACTCTCTCC GAGAGAACATACTGTTTGGGCGCCCCCTGCAGGAACATTGCTACAAGGCGGTGATGGAAGCCTGTGCCCT CCTTCCGGATTTGGAAATCCTTCCCAGTGGGGACCTCACAGAGATTGGTGAGAAGGGTGTGAACCTGTCG GGGGGCCAGAAGCAGCGTGTGAGCCTGGCTCGGGCTGTGTATTGTAACTCTGACATCTACCTCTTGGACG ACCCCCTCTCGGCTGTGGATGCACATGTTGGGAAGCACATCTTTGAGAAGGTGGTGGGTCCCATGGGCCT ACTGAAGAACAAGACACGGATCCTGGTCACCCATGGTATCAGCTACCTGCCCCAAGTGGATGTCATCATT GTCATGAGTGGCGGCAAGATCTCAGAGATGGGATCTTATCAGGAGCTGCTAGACCGGGATGGGGCCTTTG CTGAGTTCGTGCGCACCTATGCCAACACTGAGCAGGACCTGGCTTCAGAGGATGACAGTAAGAATGGTGT CAGTGGTTTAGGGAAGGAGTCAAAGCCGGTGGAAAATGGGATACTGGTGACAGACGCAGTAGGGAAGCCC CTGCAGAGGCATCTCAGCAACTCTTCTTCCCACAGTGTGGTTACTAACCAGCAGCACAGCAGCACAGCCG AGCTGCAGAAGTCTGGAGTTAAGGAGGAGACTTGGAAGCTGATGGAAGCAGACAAGGCCCAGACAGGGCA GGTGAAGCTTTCCGTGTACTGGAACTACATGAAGGCCATTGGCCTCTGCATCTCCTTCTTGAGTATCTTC CTTTTCCTGTGCAATCATGTATCTGCACTGGCTTCTAACTATTGGCTGAGTCTCTGGACAGATGACCGCC CTGCTGTCAATGGGACTCAGGAGAACAGGAATTTTCGACTAAGTGTCTATGGGGCCTTGGGCATCTTGCA AGGTGTGGCAGTATTTGGCTATTCCATGGCTGTGTCCATTGGGGGCATCTTTGCCTCCCGTCGCCTGCAC CTAGACCTGCTACAGAATGTCCTGCGATCACCCATGAGTTTCTTTGAGCGTACACCCAGTGGGAACCTAG TGAACCGATTCTCCAAGGAGTTGGACACAGTGGACTCCATGATCCCGCAGGTCATCAAGATGTTCATGGG TTCACTCTTCAGTGTCATTGGAGCTGTCATCATCATCCTACTGGCTACGCCCATTGCCGCAGTCATCATC CCACCCTTGGGTCTGGTTTACTTCTTTGTGCAGAGGTTCTATGTGGCCTCCTCTCGACAGCTGAAGCGCC TGGAGTCTGTCAGTCGTTCCCCTGTGTACTCACACTTCAATGAGACCTTGCTGGGGGTCAGTGTCATCCG TGCCTTTGAGGAACAGGAGCGCTTCATTCGCCAAAGTGACCTGAAAGTAGATGAGAACCAGAAGGCCTAC TACCCCAGCATTGTGGCCAACAGGTGGCTTGCTGTGCGCCTGGAGTGTGTGGGCAACTGCATTGTGCTGT TTGCTGCCCTTTTCGCAGTCATCTCCCGGCATAGCCTCAGTGCTGGCTTGGTGGGTCTCTCTGTGTCTTA CTCACTGCAGATAACTGCATACTTGAACTGGCTAGTTCGAATGTCCTCTGAGATGGAGACCAACATTGTG GCAGTGGAGAGACTGAAGGAATATTCTGAAACGGAGAAGGAGGCTTCTTGGCAAATCCAAGAGACAGCTC CACCCAGCACCTGGCCCCATTCAGGCCGTGTAGAGTTCCGGGATTACTGCTTGAGGTATCGAGAAGACTT GGACTTGGTTCTCAAGCACATAAATGTCACCATTGAGGGTGGAGAAAAGGTTGGTATTGTGGGTCGTACA GGAGCTGGGAAATCATCTCTCACCCTGGGTTTGTTCCGGATCAATGAGTCTGCAGAAGGGGAGATCATCA TTGATGGGATAAACATTGCTAAGATTGGCCTGCACAACCTGCGCTTCAAGATCACCATCATTCCACAGGA TCCTGTTTTGTTCTCGGGTTCCCTCCGCATGAACCTGGACCCTTTCAGTCAGTATTCTGATGAAGAAGTC TGGATGGCTCTGGAGCTTGCTCACCTGAAGGGCTTTGTGTCAGCCTTGCCTGACAAGCTGAACCATGAGT GTGCAGAAGGTGGAGAGAATCTGAGTGTGGGGCAGCGACAGCTTGTGTGCCTGGCCCGGGCTTTGCTGAG GAAGACAAAGATTCTAGTGTTGGACGAGGCTACAGCAGCTGTGGATCTGGAGACAGATGACCTTATTCAG TCCACCATCCGGACGCAGTTTGAAGACAGTACTGTGCTCACTATTGCTCATCGGCTGAATACCATAATGG ACTATACAAGGGTGATTGTCCTGGACAAAGGAGAAATTCGGGAGTGTGGTGCACCCTCTGAGCTCCTGCA GCAAAGAGGCGTCTTCTATAGCATGGCCAAGGATGCTGGCTTGGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR200856 representing NM_022281 Red=Cloning site Green=Tags(s) MALSSFCSSDGSDPLWDWNVTWHTSNPDFTKCFQNTVLTWVPCFYLWSCFPLYFLYLSRHDRGYIQMTHL NKAKTALGFFLWIICWADLFYSFWERSQGMLLAPVLLVSPTLLGITMLLATFLIQFERRKGVQSSGIMLT FWLVALLCALAILRSKIISALKKDAQVDMFRDSAFYLYFTLVFIQLVLSCFSDSSPLFSETVRDPNPCPE SSASFLSRITFWWITGMMVQGYRQPLKSSDLWSLNKEDTSEEVVPVLVNNWKKECVKSRKQPVRIVYAPP KDPTKPKGSSQLDVNEEVEALIVKSSHKDRDPSLFKVLYKTFGPYFLMSFLYKALHDLMMFAGPEILELI INFVNDREAPDWQGYLYTALLFVSACLQTLALHQYFHICFVTGMRIKTAVVGAVYRKALVITNSARKSST VGEIVNLMSVDAQRFMDLATYINMIWSAPLQVTLALYFLWLNLGPSVLAGVAVMILMVPFNAVMAMKTKT YQVAHMKSKDNRIKLMNEILNGIKVLKLYAWELAFQDKVMNIRQEELKVLKKSAYLAAVGTFTWVCTPFL VALSTFAVFVTVDEKNILDAKKAFVSLALFNILRFPLNILPMVISSIVQASVSLKRLRIFLSHEELEPDS IERWSIKDGGGMNSITVKNATFTWARDEPPTLNGITFAIPDGALVAVVGQVGCGKSSLLSALLAEMDKVE GHVTLKGSVAYVPQQAWIQNDSLRENILFGRPLQEHCYKAVMEACALLPDLEILPSGDLTEIGEKGVNLS GGQKQRVSLARAVYCNSDIYLLDDPLSAVDAHVGKHIFEKVVGPMGLLKNKTRILVTHGISYLPQVDVII VMSGGKISEMGSYQELLDRDGAFAEFVRTYANTEQDLASEDDSKNGVSGLGKESKPVENGILVTDAVGKP LQRHLSNSSSHSVVTNQQHSSTAELQKSGVKEETWKLMEADKAQTGQVKLSVYWNYMKAIGLCISFLSIF LFLCNHVSALASNYWLSLWTDDRPAVNGTQENRNFRLSVYGALGILQGVAVFGYSMAVSIGGIFASRRLH LDLLQNVLRSPMSFFERTPSGNLVNRFSKELDTVDSMIPQVIKMFMGSLFSVIGAVIIILLATPIAAVII PPLGLVYFFVQRFYVASSRQLKRLESVSRSPVYSHFNETLLGVSVIRAFEEQERFIRQSDLKVDENQKAY YPSIVANRWLAVRLECVGNCIVLFAALFAVISRHSLSAGLVGLSVSYSLQITAYLNWLVRMSSEMETNIV AVERLKEYSETEKEASWQIQETAPPSTWPHSGRVEFRDYCLRYREDLDLVLKHINVTIEGGEKVGIVGRT GAGKSSLTLGLFRINESAEGEIIIDGINIAKIGLHNLRFKITIIPQDPVLFSGSLRMNLDPFSQYSDEEV WMALELAHLKGFVSALPDKLNHECAEGGENLSVGQRQLVCLARALLRKTKILVLDEATAAVDLETDDLIQ STIRTQFEDSTVLTIAHRLNTIMDYTRVIVLDKGEIRECGAPSELLQQRGVFYSMAKDAGLV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_022281
ORF Size	4596 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_022281.2, NP_071617.2
RefSeq Size	4981 bp
RefSeq ORF	4599 bp
Locus ID	24565
UniProt ID	Q8CG09
MW	171.5 kDa
Gene Summary	ATP-binding cassette (ABC) multiple drug resistance protein involved in the release of glutathione synthase during oxidative stress [RGD, Feb 2006]

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