Baz2b (NM_001108260) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR200759 representing NM_001108260 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCTGGAGAACGGTTGCCGTCCTCACCAGCCCCTTCCACTACACCAACATCGTCGGCTCCTTCCG TGGCTTCAGCCGTTTCTAAGAGCAGCCTTCCCACGGGAGCTGCTTCACTTGGCTCTGCAGCCAGCCCATG TGTTCTTGAAGCTGGGAAGTCCAAGATCAAGGTTTCACCTGACTCAGTGTCTGGACATTTATTCAGAGCA GCTGGGGATCAGCCGTTTAACCTGTCCACAGTGCCGAGTGCCTTCCCAATGGTCAGCCACCCAGTCTTTG GTCTACATTCAGCCAGCTCAGGGCATTCAGAATTTGGTGGCTTGGGGACACTTGGTACACCCACAGCCTT AGCCGTACATCCCCAGCTAACATCTTTTCCAGGTGCAGAGTGGTGGCGAACCACAGATGGTCACTCTCGT GCAGGAACGCCCTTCTTTCCTCCATTACTGGGAATTCCACCACTGTTTGCTCCTCCAGCCCAGAATCACG ATTCGTCATTCCATTCAAGGACTTCTGGGAAGAGCAATCGAAATGGTCCCGAAAAAGGTATAAATGGGTC AGTTAATGGAAACAGTGCAGCATCTGTACATGGTGTCAATACATCTGGACTGTCCACTCCTGCTTCAAGT TCCATGGGACAGAATCAAAGCACAAGCTCAGGTGGAGGAAACCTAAAATGTCATCAGGAACAAAACAAAA GCCAGCCTGTGGATGCCAGAGCCGACAAAATCAAAGATAAGAAACCAAGGAAGAAGGCTATGGAAAGTTC TAGCAACAGTGATAGTGACTCAGGCACATCGTCAGACACCTCGAGTGAAGGCATTAGCAGCAGTGACTCT GACGATCTGGAAGAGGAAGAGGAGGAAGATCAAAGTATTGAGGAGAGTGAAGACGATGATTCTGACTCAG AGACTGAAGCACAACACAAAAGTAACAACCAGGTGCTATTACATGGTATTTCAGACCCCAAAACAGATGG ACAGAAAGCAAGTGAAAAAGCCCAGGAAAGAAGAACACACCAGCCATTACCTCCTGTGTCTGAGCCCCAG ACGCACCCATCATTCCAGTCCCAGCAGAAGCAGCCTCAGGTTCTGTCACAGCAGCTTCCATTTATTTTCC AAAGCTCTCAGGCAAAGGAGGAGTCTGTGACCAAACACACCAGTGTAATACAGTCGACGGGATTGGTGTC CAATGTGAAACCTTTGTCTTTGGTAAATCAAGCCAAAAAGGAACCTTACAGGAAACTCGTAGTTCCTTCT CCTGATGTACTTAAAGCAGGGAATAAAAATACCTCTGAAGAATCTAGTTCTTTGACCAGTGAATTGCGAT CCAAACGGGAACAATATAAGCAGACATTCCCATCCCAAGTAAAGAAACAGGAGTCGGGGAGGAGCCTGAA GAAGGTTATTGCAGCTTTGTCAAGTACGAAAGCAACCTCTAGTCCACCAGCACATCCAAAACTACCACTA GACAACAACCACCCTAATCCATTTTTGACAAATGCACTTTTAGGGAATCACCAACCAAATGGAGTCATTC AGAGCGTCATTCAAGAAGCTCCGTTAGCACTAACTACCAAAACGAAAATGCAGAGCAAGATTAACGAGAA CGTCTCCACTAGCACCCCCTTCTCCTCACCTGTCAATCTGAGCACGGGTGGGAGGAGAACTCCTGGCAGT CAGGCCCCTGCGCTGCCCTCCGCCTCACCCATCCTGCACAGTAGCGGGAAGGAGAAGCGCGTCAGCACTG ACGCAGCCCCGCTGAAAGGGCACCATCACCCCCATCCGGCAACGTCGTTGGTGGAACAGTTTAGAGGAAC AGATTCAGACATTCCCAGTAGTAAAGACTCGGAGGATTCAAATGAGGATGAGGAGGAAGATGAGGAGGAG GAAGATGAAGAGGATGATGAAGACGACGAGTCTGATGACAGTCAATCAGAATCAGATAGTAATTCAGAAT CAGATTCAGAAGGCTCAGAAGACGACGATGAGAAAGACCAGGAGGAATCAGATAGTGACACTGAAGGCGC GAAACCTGCCGTGAACCTGACTCAGACAAGCTCCTCTGCCAAAAGCCCTCCCTCCAGTCTCACAGCGCAC TCAGCACCTCACAACCTCCACATAGGAGGCCCCCCAGGCTCTGCTCCTGCTGCCCTGTGCTCTGAGTCCC AGCCTCCTGCTTTTCTTGGCACTTCTTCTTCCACACTCACTTCAAGTCCACACTCTGGTACGTCCAAACG AAGAAGAGTGGCCGATGACCACGAGCTGCGCATTCCTCTGGACTACGGCTGGCAGAGAGAGACCAGGGTG AGGAACTTCGGTGGGCGCCTTCCAGGAGAAGTCACGTATTATGCCCCGTGTGGAAAGAAGCTCAGGCAGT ACCCTGACATGATAAAGGGAATGCAGTGGTGTCTTTTGAAAGAAGAGGATGTCATTCCTCGTATCAGGGC AATGGACGGCCGAAGAGGAAGACCACCCAATCCAGATAGACCAAGAGCAAGAGAGGAATCCAGGATGAAA CGCCGGAAGGGTCGCCCTCCCAATGTTGGCAGTGCCGAATTCCTAGATAACACAGATGCAAAACTACTAA GGAAACTGCAGGCTCAAGAAATAGCCAGACAGGCAGCACAAATAAAACTTCTGAGAAAACTTCAAAAGCA GGAACAGGCACGGGTTGCCAAAGAAGCTAAAAAACAACAAGCAATAATGGCTGCTGAAGAGAAGCGGAAG CAGAAAGAACAAATGAAGATGCTGAAGCAGCAGGAAAAAATTAAGAGAATACAGCAAATCAGAATGGAAA AAGAACTCCGAGCTCAGCAGATTCTTGAGGCTAAAAAGAAAAAGAAGGAAGAAGCAGCAAATGCCAAATT ATTGGAGGCCGAGAAACGAACCAAGGAGAAAGAACTGAGAAGACAGCAGGCTGTCCTTCTGAAACACCAG GAGTTGGAGAGGCATAGACTAGATATGGAACGGGAGCGAAGGCGACAGCATATGATGCTTATGAAAGCTA TGGAAGCTCGTAAAAAAGCAGAAGAAAAAGAACGATTGAAGCAAGAGAAACGTGATGAGAAAAGATTGAA TAAGGAACGCAAACTAGAGCAACGGAGATTGGAGTTAGAAATGGCGAAGGAGCTTAAGAAACCCAAGGAA GACATGTGCTTAGCAGACCAAAAGCCCTTGCCAGAGTGGCCTCGCATCCCGGGACTTGTGCTCTCTGGAA CCACATTTTCAGACTGTCTCATGGTGGTGCAGTTCTTACGAAACTTTGGTAAAGTTTTGGGCTTTGATGT GAATATTGATGTTCCAAACCTCAGTGTTCTTCAAGAGGGATTGCTAAATATAGGGGACAGCATGGGTGAA GTACAAGACTTGCTTGTGAGGCTCCTCTCAGCTGCTGTATGTGATCCAGGTCTAATTACAGGATACAAGG CAAAAACCGCTCTTGGAGAACATTTGCTGAATGTTGGTGTGAACCGAGACAATGTGTCCGAGGTTTTGCA GATTTTCATGGAAGCCCACTGTGGACAGACTGAGCTGACCGAGAGCCTGAAGACTAAAGCGTTTCAGGCT CACACTCCAGCACAGAAAGCGTCCATCCTGGCCTTCCTTGTTAATGAGCTGGCGTGTAGCAAGAGTGTGG TGAGTGAGATTGACAAGAACATTGAGTACATGTCCAACTTGAGAAGAGACAAGTGGGTGGTGGAAGGTAA ACTCCGCAAGCTTAGGATAATCCATGCTAAGAAAACAGGCAAGAGAGATGCTTCAGGGGGCATTGATCTT GGGGAAGAGCAACATCCCTTGGGCACGCCGACTCCAGGACGCAAGCGGAGAAGGAAGGGAGGAGACAGTG ATTATGATGATGATGACGATGATGACAGCGATGACCAAGCCGATGAGGATGAGGAAGATGACGAAGATAA GGATGACAAAAAAGGAAAGAAGACCGATATCTGCGAAGATGAGGATGAAGGTGACCAAACAGCCAGTGTT GAGGAGCTCGAAAAGCAGATTGAAAAACTGAGCAAGCAACAGAGCCAGTACAGACGGAAGCTCTTTGATG CTTCTCACTCATTACGCTCGATGATGTTTGGCCAGGACCGATACCGACGCAGATACTGGATTCTTCCTCA GTGTGGAGGGATTTTTGTAGAAGGCATGGAAAGCGGTGAAGGCCTAGAAGAAATTGCAAAAGAAAAAGAA AAGCTTAAAAAGGCCGAGAGTCTCCAGATCAAAGAAGAAGTGTTCGAGACTTCTACAGACACTTTAAACT GTCCCACCAGAGATCACTGTGAGCAGAAGGACGACCTGAAAGAGAAAGATAACACGAATCTATTTCTTCA GAAACCGGGCTCGTTCTCCAAGCTAAGCAAGCTTTTGGAGGTAGCTAAAATGCCTCCCGAGTCAGATGTT ATGACTCCCCCAAAAGTGAACGTTAGTACAAATGGGGGCCCACTGTCTCATCAGAACAGCGGGAAGCATG CACTGGGAAGCATTCCCTCAGCAGCGGCACAGAGCCCCGTGGGGAAGACAGACGTCAGTCTGTTCAGCTC GGGCTCTGGTAGTTGTGGGAAGTTCTACAGTCCTCTCCCCAATGACCAGCTGTTAAAAACGCTGACTGAA AAGAACCGGCAGTGGTTTAGCCTTTTGCCAAAAACACCTTGTGATGACACGTCCCTGACCCACGCTGACC TGTCCGCCAGTTCGGTGACTCCTCAGTCTCAGCCACCATCTAAGTCACCGTCGCCTGCTCCAGCTCCACT GCTCGCATCCTCGTCCGTTCAGAACCCGGCCGGACTGAACCCGTTTGCTCTGTCACCTCTTCAGGTGAAG AGTGGTGTGTCTATGATGGGACTTCAGTTCTGTGGGTGGCCTGCTGGTGTGCTCACTTCCAGTGTCCCAT TTACATCACCCTTGCCTGGTCTCGGCTCAGGGTTGGGACTGCCAGAAGGAAATGGTAGCAGCTCATTCTT GAATTCCAGTGTTGCTTCCAGTAAAAGTGAATCTCCAATCCCTCCAGCTGAAAAGGCCTCCACCGCCACA CCAGCCGCTGTTGAAGTAGCAAAACCAGTAGATTTTCCTATTCCGAAGCCTATCCCAGAAGAGATGCAGC TCGGCTGGTGGAGAATCATGGACCCCGAGGACCTGAGGACGTTGCTGAAGGTGCTGCACCTCCGAGGGCT ACGGGAAAAGGCCCTGCAGAAGCAAGTGCAGAAGCACCTGGACTACATTACTCACGCCTGTGCCAAGAAT AAGGACGTTGCTATTATTGAATTAAATGAGAATGAAGAAAATCAGGTAACGCGCGATTTAGTGGAGAACT GGTCGGTAGAAGAGCAGGCGATGGAGCTGGACCTGAGCATTCTCCAGCAAGTGGAGGACCTGGAACGCAG GGTTGCATCCGCAAGCTTGCAAGTGAAGGGTTGGATGTGTCCAGAGCCTGCGTCAGAAAGGGAGGACTTG GTATATTTTGAACATAAGTCATTTACTAAGTTGTCCAAGGAGCATGATGGAGAACTTACTGGTGACGAAG AAAACAGTGCTCATGCACTGGCACGGAAGAGTGACAACCCCCTAGATATAGCTGTAACCAGGCTGGCTGA TTTGGAGCGGAACATTGAGAGAAGGTATCTGAAGAGCCCCTTAAGTACCACCATTCAGATCAAACTGGAT AATGTGGGCACAGTTACTGTCCCTGCTCCTGCACCATCCATTAGTGGTGATGGTGACGGAGTTGAAGAGG ACATTGCTCCAGGGCTCAGGGTCTGGAGAAGGGCGTTAGCAGAGGCTCGCAGTGCTGCACAGGTAGCCCT GTGCATCCAGCAGTTACAGAGATCAATAGCATGGGAAAAATCAATTATGAAAGTTTACTGCCAGATCTGT CGAAAGGGAGACAATGAGGAACTGCTGCTACTCTGTGACGGCTGTGACAAAGGCTGCCATACCTACTGCC ACAGGCCCAAGATTACAACTATTCCTGACGGGGACTGGTTTTGTCCAGCTTGCATCGCTAAGGCAAGCGG TCAGAGTATAAAAATTAAAAAAATTCATATCAAAGGAAAAAAGACAAATGAATCCAAGAAAAGCAAGAAA GGAAACGTCGCTGGGGACACTGAAGACGAAGACTCCACTTCCACAAGCAGTTCACTCAAACGAGGAAGCA AAGATCTCAAAAAGAGGAAAATGGAGGAAAACACTTCCCTCCACTTGTCAAAAGCAGAAAGTGCTACTTC CGTTAAGAAACCGAAAAAAGAAGAGTGCAAGGACCTGGCTCTCTGCAGCATGATCCTGACTGAAATGGAA ACGCATGAGGACGCCTGGCCCTTTCTGCTTCCGGTCAACCTGAAGCTCGTACCCGGCTACAGGAAGGTTA TTAAGAAGCCTATGGATTTTTCTACAATTAGAGAAAAACTAAATAATGGACAGTATCCCAACTTTGAGAC CTTTGCTCTAGACGTCAGGCTTGTTTTTGACAACTGTGAAACATTTAATGAAGATGACTCGGACATAGGC AGAGCTGGCCACAGTATGCGAAAATACTTTGAAAAAAAGTGGACAGATACCTTCAAAGTGAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR200759 representing NM_001108260 Red=Cloning site Green=Tags(s) MESGERLPSSPAPSTTPTSSAPSVASAVSKSSLPTGAASLGSAASPCVLEAGKSKIKVSPDSVSGHLFRA AGDQPFNLSTVPSAFPMVSHPVFGLHSASSGHSEFGGLGTLGTPTALAVHPQLTSFPGAEWWRTTDGHSR AGTPFFPPLLGIPPLFAPPAQNHDSSFHSRTSGKSNRNGPEKGINGSVNGNSAASVHGVNTSGLSTPASS SMGQNQSTSSGGGNLKCHQEQNKSQPVDARADKIKDKKPRKKAMESSSNSDSDSGTSSDTSSEGISSSDS DDLEEEEEEDQSIEESEDDDSDSETEAQHKSNNQVLLHGISDPKTDGQKASEKAQERRTHQPLPPVSEPQ THPSFQSQQKQPQVLSQQLPFIFQSSQAKEESVTKHTSVIQSTGLVSNVKPLSLVNQAKKEPYRKLVVPS PDVLKAGNKNTSEESSSLTSELRSKREQYKQTFPSQVKKQESGRSLKKVIAALSSTKATSSPPAHPKLPL DNNHPNPFLTNALLGNHQPNGVIQSVIQEAPLALTTKTKMQSKINENVSTSTPFSSPVNLSTGGRRTPGS QAPALPSASPILHSSGKEKRVSTDAAPLKGHHHPHPATSLVEQFRGTDSDIPSSKDSEDSNEDEEEDEEE EDEEDDEDDESDDSQSESDSNSESDSEGSEDDDEKDQEESDSDTEGAKPAVNLTQTSSSAKSPPSSLTAH SAPHNLHIGGPPGSAPAALCSESQPPAFLGTSSSTLTSSPHSGTSKRRRVADDHELRIPLDYGWQRETRV RNFGGRLPGEVTYYAPCGKKLRQYPDMIKGMQWCLLKEEDVIPRIRAMDGRRGRPPNPDRPRAREESRMK RRKGRPPNVGSAEFLDNTDAKLLRKLQAQEIARQAAQIKLLRKLQKQEQARVAKEAKKQQAIMAAEEKRK QKEQMKMLKQQEKIKRIQQIRMEKELRAQQILEAKKKKKEEAANAKLLEAEKRTKEKELRRQQAVLLKHQ ELERHRLDMERERRRQHMMLMKAMEARKKAEEKERLKQEKRDEKRLNKERKLEQRRLELEMAKELKKPKE DMCLADQKPLPEWPRIPGLVLSGTTFSDCLMVVQFLRNFGKVLGFDVNIDVPNLSVLQEGLLNIGDSMGE VQDLLVRLLSAAVCDPGLITGYKAKTALGEHLLNVGVNRDNVSEVLQIFMEAHCGQTELTESLKTKAFQA HTPAQKASILAFLVNELACSKSVVSEIDKNIEYMSNLRRDKWVVEGKLRKLRIIHAKKTGKRDASGGIDL GEEQHPLGTPTPGRKRRRKGGDSDYDDDDDDDSDDQADEDEEDDEDKDDKKGKKTDICEDEDEGDQTASV EELEKQIEKLSKQQSQYRRKLFDASHSLRSMMFGQDRYRRRYWILPQCGGIFVEGMESGEGLEEIAKEKE KLKKAESLQIKEEVFETSTDTLNCPTRDHCEQKDDLKEKDNTNLFLQKPGSFSKLSKLLEVAKMPPESDV MTPPKVNVSTNGGPLSHQNSGKHALGSIPSAAAQSPVGKTDVSLFSSGSGSCGKFYSPLPNDQLLKTLTE KNRQWFSLLPKTPCDDTSLTHADLSASSVTPQSQPPSKSPSPAPAPLLASSSVQNPAGLNPFALSPLQVK SGVSMMGLQFCGWPAGVLTSSVPFTSPLPGLGSGLGLPEGNGSSSFLNSSVASSKSESPIPPAEKASTAT PAAVEVAKPVDFPIPKPIPEEMQLGWWRIMDPEDLRTLLKVLHLRGLREKALQKQVQKHLDYITHACAKN KDVAIIELNENEENQVTRDLVENWSVEEQAMELDLSILQQVEDLERRVASASLQVKGWMCPEPASEREDL VYFEHKSFTKLSKEHDGELTGDEENSAHALARKSDNPLDIAVTRLADLERNIERRYLKSPLSTTIQIKLD NVGTVTVPAPAPSISGDGDGVEEDIAPGLRVWRRALAEARSAAQVALCIQQLQRSIAWEKSIMKVYCQIC RKGDNEELLLLCDGCDKGCHTYCHRPKITTIPDGDWFCPACIAKASGQSIKIKKIHIKGKKTNESKKSKK GNVAGDTEDEDSTSTSSSLKRGSKDLKKRKMEENTSLHLSKAESATSVKKPKKEECKDLALCSMILTEME THEDAWPFLLPVNLKLVPGYRKVIKKPMDFSTIREKLNNGQYPNFETFALDVRLVFDNCETFNEDDSDIG RAGHSMRKYFEKKWTDTFKVS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001108260
ORF Size	6573 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001108260.1, NP_001101730.1
RefSeq Size	7132 bp
RefSeq ORF	6576 bp
Locus ID	317627
MW	241.3 kDa

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