Abcc12 (NM_199377) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR200649 representing NM_199377 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGGCGAAGACCCGTACCTTATCTCAGACCTGGATCGCAGAGGCCGTCGGAGATCCTTTGCCGAGA GATATGATCCCAGCCTGAAAACCATGATCCCAATGCGACCCTATGCAAGGCTGGCACCCAATCCTGTGGA TGATGCGGGGCTGCTGTCCTTCGCCACGTTTTCCTGGCTCACACCTGTGATGATTCGAAGTTACAAGCAC ACGCTGACCGTGGACACCCTGCCTCCACTATCTCCTTACGACTCATCGGATGTCAATGCCAAGAGACTCC AAATCCTTTGGGATGAAGAAATAGAAAGGGTAGGGCCCGAGCGGGCCTCTTTGGGCCGTGTGGTTTGGAA ATTCCAGAGGACCCGTGTTCTGATGGATGTTGTAGCCAACATTCTCTGCATCATCATGGCAGCCTTAGGG CCGACAGTTCTCATTCACCAAATCCTCCAGCACGTTACCAACATCTCCTCCGGACACATCGGGATCAGCA TCTGCTTGTGTCTGGCCCTTTTTGCCACCGAGTTTACCAAAGTCCTCTTTCGGGCCCTTGCCTGGGCCAT AAATTACCGCACAGCGATTCGACTGAAGGTGGCCCTGTCCACGCTAATTTTCAAAAATCTGTTGTCCTTC AAGACATTAACCCACATCTCTGCAGGCGAGGTACTCAATGTACTATCGAGTGATAGTTATTCCTTGTTTG AGGCTGCCTTGTTTTGTCCTTTGCCAGCCACCATCCCGATCCTAATGGTCGTTTGTGCCGTGTATGCCTT TTTCATTCTGGGGTCCACCGCTCTTGTTGGGATATGCGTGTATCTCATATTCATCCCGATCCAGATGTTT ATGGCCAAACTCAACTCAGCTTTCCGAAGATCAGCAATTTCAGTGACAGACAAGCGGGTTCAGACAATGA ATGAGTTTCTGACCTGCATCAAACTGATTAAAATGTATGCCTGGGAGAAATCTTTTATGAACACCATTCA CGACATAAGAAAGAGAGAGAAAAAATTACTGGAAAAGGCTGGATACGTCCAAAGTGGGAACTCAGCCCTG GCTCCCATAGTGTCTACCATAGCCATTGTGTCTACTTTCACCTGCCACATTTTCCTGAAACGCACACTCA CTGCCCCTGTGGCATTTAGTGTGATTGCCATGTTTAATGTAATGAAGTTTTCAATTGCAATCTTGCCTTT CTCGGTCAAAGCAGTGGCCGAAGCCAGTGTCTCTCTAAGGAGAATGAAGAAAATTCTTGTAGCTAAAAGT CCCCCATCCTACATCACCCAACCAGAAGACCCAGATACTATCTTGCTTTTAGCAAATGCCACCCTGACAT GGGAACAAGAAATCAACAGGAAAAGAGGCCCATCGAAGACACAAGATCAGAGAAGGCACGTTTTCAAGAA ACAGAGGGCAGAGCTATACAGTGAGCAAAGTCTCTCAGATCAGGGAGTTGCCAGCCCAGAGCGGCAAAGT GGCAGCCCCAAGTCAGTACTACACAACATCAGCTTTGTGGTGAGAAAGGGAAAGGTCTTGGGGATATGCG GGAATGTTGGAAGCGGGAAGAGTTCCCTCATTTCTGCTCTTCTAGGGCAGATGCAGTTACAGAAAGGGGT CGTGGCTGCCAGTGGACCTTTGGCTTATGTTTCTCAGCAAGCTTGGATCTTCCACGGAAATGTGAGGGAG AACATCCTGTTTGGAGAGAAGTACAACCACCAAAGGTACCAACACACGGTTCATGTCTGTGGGCTCCAGA AGGATTTGAACAGCCTCCCTTATGGAGACCTGACTGAGATTGGGGAGCGGGGTGTGAACCTCTCCGGGGG ACAGAGGCAGAGGATCAGCCTGGCCCGGGCTGTGTACGCCAACCGTCAGCTCTACCTGCTGGATGACCCG CTCTCGGCAGTGGATGCCCATGTGGGGAAGCACGTCTTTGAAGAATGCATTAAAAAGACACTCAAGGGGA AGACTGTGGTGCTGGTTACCCACCAGCTGCAGTTCCTGGAGTCTTGCGATGAGGTCATTCTGTTAGAAGA TGGAGAGATCTGTGAAAAGGGCACCCACAAGGAGTTGATGGAAGAGAGAGGCCGCTATGCAAAGCTGATC CACAACCTCAGGGGACTGCAATTCAAGGATCCAGAGCACATTTACAATGTAGCCATGGTGGAGACCCTGA AGGAGAGCCAAGCTCAAAGGGATGAAGACGCTGTCTTGGCTTCAGGAGATGAGAGAGATGAAGGAAAAGA ACCCGAGACAGAAGAATTTGTGGACATAAAAGCTCCCGTGCACCAGCTCATCCAGATTGAATCCCCCCAA GAAGGAATCGTGACCTGGAAAACATATCACACGTATATTAAAGCTTCTGGAGGGTACCTGGTCTCCTTCC TGGTTTTGTGTCTCTTCTTCCTGATGATGGGCAGCTCTGCCTTCAGCACCTGGTGGCTGGGGCTCTGGTT GGACAGTGGCTCCCAGGTCATCTGTGCACCCCAGAGCAATGAGACAGCCTGCAACGTTAACCAGACGCTG CAGGACACCAAACACCACATGTACCAGTTGGTTTACATAGCAAGCATGATGTCTGTGCTGACGTTTGGCA TCATCAAAGGCTTCACCTTCACCAACACGACACTGATGGCGTCCTCCTCCCTCCACAACAGAGTGTTTAA CAAGATCGTCAGCAGCCCAATGAGCTTCTTTGACACGACTCCCACAGGCCGGCTAATGAACCGCTTTTCT AAAGATATGGATGAGCTAGATGTGAGGCTGCCATTTCACGCTGAGAATTTTTTGCAGCAGTTTTCTATGG TGGTGTTTATTCTGGTGATTATGGCTGCCTCGTTTCCTGTTGTCCTCGTGGTGCTGGCTGGCCTGGCTAT TCTCTTCTTCATCCTTTTACGCATTTTCCATCGAGGGGTGCAGGAGCTCAAGCAGGTGGAGAACATCAGC CGGTCCCCTTGGTTCTCTCACATCACCTCCTCCATGCAGGGTCTGGGTGTCATCCATGCCTACGACAAAA AGGACGATTGCATCAGCAAGTTTAAGGCGCTCAATGATGAAAACTCCAGCCACCTCTTGTACTTTAACTG TGCACTCAGGTGGTTTGCTCTCAGAATGGACATCCTCATGAACATCGTCACCTTTGTCGTGGCCTTGCTG GTGACCCTGAGCTTTTCTTCAATCAGTGCTTCATCCAAAGGCTTGTCCCTGTCTTACATCATCCAGCTCA GTGGACTGCTTCAAGTGTGTGTGCGAACAGGAACAGAGACCCAAGCCAAGTTCACCTCTGCAGAGCTAAT GAGGGAGTACATTTCGACCTGTGTCCCTGAACACACTCAATCCTTCAAAGTGGGGACCTGTCCCAAAGAC TGGCCGAGCCGTGGGGAGATAACTTTCAAGGACTATCGGATGAGATACAGAGACAACACCCCTCTCGTTC TCGATGGGTTGAACCTGAACATCCAAAGCGGGCAGACAGTTGGGATTGTGGGAAGAACGGGCTCTGGTAA ATCATCACTGGGCATGGCCCTGTTTCGTCTGGTGGAACCAGCCTCTGGCACCATCTTCATTGACGAGGTG GATATCTGCACTGTGGGTCTGGAAGAGCTCCGAACCAAGCTGACCATGATCCCCCAGGATCCTGTCCTGT TTGTAGGTACAGTAAGGTACAACTTGGATCCCTTGGGGAGTCACACCGACGAGATGCTCTGGCATGTTTT GGAGAGAACATTCATGAGAGACACAATAATGAAACTCCCAGAGAAATTACAGGCAGAAGTCACAGAAAAC GGGGAAAACTTCTCAGTAGGAGAACGCCAGCTGCTTTGTATGGCCCGGGCACTTCTCCGTAATTCAAAAA TCATTCTCCTTGATGAAGCTACTGCCTCCATGGATTCCAAGACGGACACCCTTGTTCAGAGCACCATAAA AGAGGCCTTCAAAAGCTGCACAGTGCTGACCATCGCTCATCGCCTAAACACTGTTCTCAACTGTGACCTT GTCCTGGTCATGGAAAATGGGAAGGTGATTGAGTTTGACAAGCCTGAAGTCCTCGCTGAGAAGCCTGACT CTGCATTTGCGATGTTACTAGCTGCAGAAGTTGGACTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR200649 representing NM_199377 Red=Cloning site Green=Tags(s) MVGEDPYLISDLDRRGRRRSFAERYDPSLKTMIPMRPYARLAPNPVDDAGLLSFATFSWLTPVMIRSYKH TLTVDTLPPLSPYDSSDVNAKRLQILWDEEIERVGPERASLGRVVWKFQRTRVLMDVVANILCIIMAALG PTVLIHQILQHVTNISSGHIGISICLCLALFATEFTKVLFRALAWAINYRTAIRLKVALSTLIFKNLLSF KTLTHISAGEVLNVLSSDSYSLFEAALFCPLPATIPILMVVCAVYAFFILGSTALVGICVYLIFIPIQMF MAKLNSAFRRSAISVTDKRVQTMNEFLTCIKLIKMYAWEKSFMNTIHDIRKREKKLLEKAGYVQSGNSAL APIVSTIAIVSTFTCHIFLKRTLTAPVAFSVIAMFNVMKFSIAILPFSVKAVAEASVSLRRMKKILVAKS PPSYITQPEDPDTILLLANATLTWEQEINRKRGPSKTQDQRRHVFKKQRAELYSEQSLSDQGVASPERQS GSPKSVLHNISFVVRKGKVLGICGNVGSGKSSLISALLGQMQLQKGVVAASGPLAYVSQQAWIFHGNVRE NILFGEKYNHQRYQHTVHVCGLQKDLNSLPYGDLTEIGERGVNLSGGQRQRISLARAVYANRQLYLLDDP LSAVDAHVGKHVFEECIKKTLKGKTVVLVTHQLQFLESCDEVILLEDGEICEKGTHKELMEERGRYAKLI HNLRGLQFKDPEHIYNVAMVETLKESQAQRDEDAVLASGDERDEGKEPETEEFVDIKAPVHQLIQIESPQ EGIVTWKTYHTYIKASGGYLVSFLVLCLFFLMMGSSAFSTWWLGLWLDSGSQVICAPQSNETACNVNQTL QDTKHHMYQLVYIASMMSVLTFGIIKGFTFTNTTLMASSSLHNRVFNKIVSSPMSFFDTTPTGRLMNRFS KDMDELDVRLPFHAENFLQQFSMVVFILVIMAASFPVVLVVLAGLAILFFILLRIFHRGVQELKQVENIS RSPWFSHITSSMQGLGVIHAYDKKDDCISKFKALNDENSSHLLYFNCALRWFALRMDILMNIVTFVVALL VTLSFSSISASSKGLSLSYIIQLSGLLQVCVRTGTETQAKFTSAELMREYISTCVPEHTQSFKVGTCPKD WPSRGEITFKDYRMRYRDNTPLVLDGLNLNIQSGQTVGIVGRTGSGKSSLGMALFRLVEPASGTIFIDEV DICTVGLEELRTKLTMIPQDPVLFVGTVRYNLDPLGSHTDEMLWHVLERTFMRDTIMKLPEKLQAEVTEN GENFSVGERQLLCMARALLRNSKIILLDEATASMDSKTDTLVQSTIKEAFKSCTVLTIAHRLNTVLNCDL VLVMENGKVIEFDKPEVLAEKPDSAFAMLLAAEVGL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_199377
ORF Size	4098 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_199377.1, NP_955409.1
RefSeq Size	4541 bp
RefSeq ORF	4101 bp
Locus ID	291923
UniProt ID	Q6Y306
MW	153 kDa
Gene Summary	member of the ATP-binding cassette (ABC) transporter family; human homolog is a candidate disease gene for paroxysmal kinesigenic choreoathetosis [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.