Sycp2 (NM_130735) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR200629 representing NM_130735 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGTGAGACCAGACCCCCAACAGTTAGAAAAGTGTATTGATGATGCTTTGAGAAAAAACGACTTCA AGCCTTTGGTGACACTTTTACAAATTGATATTTGTGAAGATGTGAAGATTAAATGCAGCAAACAATTCCT CCGCAAGTTGGATGACTTAATATGCAGGGAACTTCATAAAAAGGATATCCAAACTATTTCAAACATCTTG ATATCTATTGGAAGATGTAGCAAGAATATCTTTATATTGGGACAAACTGGACTTCAAACCATGATAAAAC AAGGATTAGTCCAAAAGATGGTTTCCTGGTTTGAAAATTCCAAGGAGATTATTCTGAGTCAGCGACAATC AAAAGATGAAGCTGTTATGAATATGATAGAAGACTTATTTGATCTTTTGATGGTCGTATATGACGTCAAT GATGAAGGTAAAAACCAAGTATTGGAAAGTTTCATACCTCACATCTGTGCCCTGGTTATTGATTCAAGAG TGAATTTTTGCATTCAGCAAGAGGCTTTAAAAAAAATGAATTTGATGCTTGACAGAATACCTCAAGATGC CAACAAAATACTTTGTAATCAAGAAATATTAACTCTCATGAGTAATATGGGAGAAAGGATTTTAGATGTA GGAGATTATGAATTACAGGTAGGCATTGTGGAAGCTTTGTGTAGAATGACTACCGAAAAACGGAGGCAGG AGCTGGCATATGAATGGTTTTCAATGGACTTTATTGCTAATGCATTTAAGAAAATTAAAGACTGTGAATT TGAAACAGATTGCAGAATATTTCTCAACTTGGTAAACGGCATGCTGGGTGACAGAAGAAGGGTCTTTACA TTTCCTTGCTTGTCAGCATTTCTTGGTAAATATGAGCTGCAGATACCATCAGATGAAAAACTTGAGGAAT TCTGGATTGATTTTAATCTCGGGAGCCACACTCTGTCATTCTACATTGCTGGAGATGATGATGATCACCA ATGGGAAGCTGTCACTGTGCCTGAAGAAAAAGTTGATATGTACAACATTGAAGTGAGAGAATCAAAGAAG CTACTGACTCTAACTTTGAAAAATATAGTAAAAATTAGTAAAAAAGAAGGAAAAGAGTTACTTTTGTATT TTGATGCAGCATTAGAAATCACCAATGTGACTAAAAAACTTTTTGGTGGAAATAAGTATAAGGAATTTAC CAGAAAACAAGATATTTCAGTTGCCAAAACATCCATTCATGTACTTTTTGATGCAAGTGGATCACAGATT CTAGTACCAGAAAGTCAACCATCTCCAGTCAAAGAAAACCTCATTCATCTAAAAGAGAAATCTAACCTCC AAAAGAAACTTACAAACCCTCTAGAACCAGACAACAGCAGCAGCCAGCGCGACAGGAAAAACAGCCAGGA TGAGATCACTACACCTAGCAGAAAGAAAATGTCTGAAGCATCAATGATTGTTCCCGATACAGACAGATAC ACTGTGCGAAGTCCAATACTCTTAATCAACACATCAACCCCGCGAAGAAGTAGGGCACCACTGCAAGCAA TACATTCTGCTGAGAAGGCTGTTTCTAAAACATCAGAAAGTGGAGTGGATTATGCTGTGTCACTCAAATC TAGACAATCTGATGGAAGAAATAGAGGGAACAATAGAGCCAATCATAACAAAACTGCTACAGTACAAAAC AAAGGACATGAGCACCATGAATCCCCAGACCAAACTTTCAATGAAATTGAGGAAACTCTCTCTGATGCAT ATGCAGTGGAAAAAGTAGACAAGCCTGTATTACCTGGTGTTTTAGACATCTCGAAAAATAAAGCACATTC CAGATGGGCATGTTGGACACCTGTAACAACTATCAAACTCTGCAATAACCAGAGATCCTGTGCTTTACCA GGAGATACTTTTACCCAAGATACTGGTGTCAACAAAAAATGCACTAAACAAAAATCAGTATCTGATGATG ATTCTGAAGAAACACAAAGGGTAAAATATAGTAAAGATGTCATCAAGTGTAACAAGTCAGAAGAAGCAGA AGTTTGTGAAAGAAACATTCAAGAACAAAATCATCCTAAATATTCACAAAAGAAAAATACTGCAAATGCA AAGAAGAATGATTGGCATATTGAATCTGAAACAACTTATAAATCAGTACTCCTAAATAAGACAACTGAAG AATCACTCATCTATAAGAAGACGTGTGTATTGTCAAAAGATGTGAATACTACTATCTGTGATAAAAGCCC TTCTAGAAAAAGCATGAGGAGCCATACAAAGTCAAGGAAAGAACTGATGTCTGAAGTTACTTCATGTGAG CTAGATGAAATACCAGTGAGAGAAAATTCAAAAGGGAAAAGATTTACTGGTACAGCAGAATCCTTGATAA ACCTAATTAATAAGAGGTATAACTCAAGTGATGACATGATATCAACAAGAAAACTGAAGGAGCCTCGGGA TGGCAGTGGGTTTTCAAAGAAACCTGAACTGCAGTTCAATAAGGTTCAGAGAAAGAGTTACAGAAAACTG AAGACTGTTGTTAATGTTACTTCTGAATGTCCACTGAATGATGTATATAATTTCAGCTTGAATGGAGCCG ATGAGCCTGTTATAAAACTTGGAATCCAAGAATTTCAAGCTACAACTAGAGAAGCTAGTATGGATAATTC AATAAAATTGGTAGATGTAAGGAACCGTGATGAACGTGACCTTTCTCTCAAAACAAAAGATGAAAGAATA TTAAGTCATGAGAGGAAAACTCTCTTCAGTGACACTGAAACAGAATGTGGTTGGGATGACAGCAAGACTG ACATTAGCTGGCTAAGGAAACCAAAATCAAAAAGACTAATGGATTATAGTAGAAATAAAAACACAAAAAA ATGTAAAAGTATAAAATCAAGGTCATCCACGGAAAAGGGACAGCCAAGATCCACAGTGGTACTCAGTAAA AACATTGCGAAAAATGATTATGAAGTAATTGTAGATGGGAGAACCAGACTTCCACGAAGAGCAACAAAAA CAAAAAAAAATTACAAAGATCTCTCAACTTCAGGATCAGAATCAGAGAGTGAAAAAGAAATTTCATATTT GTTTAAAGATAAACTACCAACAAAGGAGGAGACTGTCCATTCCAGCGCCCAAACAAAGAAACTGCCCAAG AAACAACAGAAAGTCTTCAATACAGAAGCGCTGAAAGGACAGCCTTCAGAAGAACAGAAAAATTCATCTA CGCTAAGAAATGGGAGAGAAGACAGTCTGTACTTGTCTTCTGCATCTGTGTCTGGGAGCTCGTCATCTGT GGAAGTGATGAGATGTACAGAGAAAATAACAGAAAGGGATTTTACTCAGGATTATGACTACATCACAAAG TCTCTTTCACCATATCCAAAAGCTGCATCACCTGAATTCTTAAACAGAAGCAATAGAGTGGTAGGTCATG GAAAATCACCAAGAATTAGTGAGACCAGTGCAGTATGTGTGAGAAAGAGTTGCTCACCTGCTTCAGGACT GCCCTTTTCGCCCAGACACACAACCAAGAATAATTCTGTTATGAATATAAAAAATACAAATTCAGTGATA AATAATCAAAGAACCCAACATTGTAACAGCTATTCAGATGTAAGCAGTAACAGTTCAGAGAAACTTTATA TGGAACCCGAATCTCCGGATAGCTGTGAAAACCATGTGCAAAGCAAGAGAGAGGAAAATCATGCAGCCTC TCCATTTTCATTGTCTAGTGAAAAAATAGAGAAAATATGGTTTGACATGCCCAATGACAATACTCATGTA TCAGGTCCCAGTCAACGTGGTAGCAAAAGACGGATGTACCTAGAAGAAGATGAGCTAAGTAATCCCAGTG AAGCAGAAGTGCAAGAGGCAGAAGAAAGGGAACATTTGGTTTCCAAAAAACTATGTCAAAGGGAACATTT CGATCAGCATACCTCTGAAACTTCATTATCAACACCAGAGTTTTCTGTTCCTAAGGACTGGCAACAGGAG TTACAAGGTGCTGGGATGTTTTATGATAACATCAACTCAGATTATAAAAGGAAAACTGACACACAACATA AAATCATGGATGATTTTACTACAAAGACATTGAAACTGACTCAACAACATCTGTTGGCAATGGCCTGTCA AGCTCGGGGACACAGGGATGAAAATATTGACAAATTCCAAGTCACTCTCCTAGATGAGCTGGAAAAAGTT GAAAAAGACTCACAGACTTTACGAGACTTGGAGAAAGAGTTTGTGGACATCGAAGAAAAGATAGTTCACA AGATGAGGGCATTTCACCAAAGTGAGCGAGAAAGGTTTCGTGCTTTGAAAACTTCATTGGATAAAAGTTT GCTTGTCTATAATTCTGTTTATGAAGAGAATGTTCTTACATCTGAGATGTGTTTGATGAAAGCAAACATG AAAATGCTACAAGACAAGCTACTGAAGGAGATGCATGAAGAGGAACTTCTCAACATACGCAGAGGACTGG AGTCATTATTCAAGGATCATGAAGGAAACAATGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR200629 representing NM_130735 Red=Cloning site Green=Tags(s) MPVRPDPQQLEKCIDDALRKNDFKPLVTLLQIDICEDVKIKCSKQFLRKLDDLICRELHKKDIQTISNIL ISIGRCSKNIFILGQTGLQTMIKQGLVQKMVSWFENSKEIILSQRQSKDEAVMNMIEDLFDLLMVVYDVN DEGKNQVLESFIPHICALVIDSRVNFCIQQEALKKMNLMLDRIPQDANKILCNQEILTLMSNMGERILDV GDYELQVGIVEALCRMTTEKRRQELAYEWFSMDFIANAFKKIKDCEFETDCRIFLNLVNGMLGDRRRVFT FPCLSAFLGKYELQIPSDEKLEEFWIDFNLGSHTLSFYIAGDDDDHQWEAVTVPEEKVDMYNIEVRESKK LLTLTLKNIVKISKKEGKELLLYFDAALEITNVTKKLFGGNKYKEFTRKQDISVAKTSIHVLFDASGSQI LVPESQPSPVKENLIHLKEKSNLQKKLTNPLEPDNSSSQRDRKNSQDEITTPSRKKMSEASMIVPDTDRY TVRSPILLINTSTPRRSRAPLQAIHSAEKAVSKTSESGVDYAVSLKSRQSDGRNRGNNRANHNKTATVQN KGHEHHESPDQTFNEIEETLSDAYAVEKVDKPVLPGVLDISKNKAHSRWACWTPVTTIKLCNNQRSCALP GDTFTQDTGVNKKCTKQKSVSDDDSEETQRVKYSKDVIKCNKSEEAEVCERNIQEQNHPKYSQKKNTANA KKNDWHIESETTYKSVLLNKTTEESLIYKKTCVLSKDVNTTICDKSPSRKSMRSHTKSRKELMSEVTSCE LDEIPVRENSKGKRFTGTAESLINLINKRYNSSDDMISTRKLKEPRDGSGFSKKPELQFNKVQRKSYRKL KTVVNVTSECPLNDVYNFSLNGADEPVIKLGIQEFQATTREASMDNSIKLVDVRNRDERDLSLKTKDERI LSHERKTLFSDTETECGWDDSKTDISWLRKPKSKRLMDYSRNKNTKKCKSIKSRSSTEKGQPRSTVVLSK NIAKNDYEVIVDGRTRLPRRATKTKKNYKDLSTSGSESESEKEISYLFKDKLPTKEETVHSSAQTKKLPK KQQKVFNTEALKGQPSEEQKNSSTLRNGREDSLYLSSASVSGSSSSVEVMRCTEKITERDFTQDYDYITK SLSPYPKAASPEFLNRSNRVVGHGKSPRISETSAVCVRKSCSPASGLPFSPRHTTKNNSVMNIKNTNSVI NNQRTQHCNSYSDVSSNSSEKLYMEPESPDSCENHVQSKREENHAASPFSLSSEKIEKIWFDMPNDNTHV SGPSQRGSKRRMYLEEDELSNPSEAEVQEAEEREHLVSKKLCQREHFDQHTSETSLSTPEFSVPKDWQQE LQGAGMFYDNINSDYKRKTDTQHKIMDDFTTKTLKLTQQHLLAMACQARGHRDENIDKFQVTLLDELEKV EKDSQTLRDLEKEFVDIEEKIVHKMRAFHQSERERFRALKTSLDKSLLVYNSVYEENVLTSEMCLMKANM KMLQDKLLKEMHEEELLNIRRGLESLFKDHEGNNA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_130735
ORF Size	4515 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_130735.1, NP_570091.1
RefSeq Size	4771 bp
RefSeq ORF	4518 bp
Locus ID	83820
UniProt ID	O70608
MW	172.6 kDa
Gene Summary	a major component of synaptonemal complexes; may be a DNA binding protein [RGD, Feb 2006]

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