Ret (NM_012643) Rat Tagged ORF Clone
CAT#: RR200483
- TrueORF®
Ret (Myc-DDK-tagged ORF) - Rat ret proto-oncogene (Ret), transcript variant 1, (10 ug)
"NM_012643" in other vectors (3)
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CNY 17,860.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR200483 representing NM_012643
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAAAGCGAGGTCCGGCGCCGCAGGGCTGGGGCTGAAGCTGTTTTTGCTGCTGCCGCTACTGGGAG AAGCCCCGCTGGGTCTCTACTTCTCAAGGGATGCTTACTGGGAGAGGCTGTATGTGGACCAGCCAGCTGG CACACCTCTGCTCTATGTCCATGCCCTACGGGATGCCCCTGGAGAAGTGCCCAGCTTCCGCCTGGGCCAG TATCTCTATGGCGTCTACCGCACGCGTCTGCATGAGAATGACTGGATCCACATCGATTCGGGCACTGGCC TCCTCTACCTCAATCAGAGCCTGGACCATAGTTCCTGGGAGCAGCTCAGCATCCGCAATGGCGGCTTCCC CTTGCTCACCGTCTTCCTCCAGGTCTTCCTGGGGTCCACAGCCCAGAGAGAGGGAGAGTGTCATTGGCCA GGCTGTGCCCGTGTGTACTTCTCCTTCATCAACGACACCTTCCCAAATTGTAGCTCCTTCAAAGCCCGGG ATCTCTGCACCCCGGAGACGGGTGTGTCCTTCCGCATCAGGGAGAACAGGCCCCCTGGCACCTTCTACCA GTTCCGCATGCTACCTGTGCAGTTCCTTTGTCCTAACATCAGTGTGAAGTACAAACTCTTAGAAGGGGAC GGTCTGCCCTTCCGTTGTGACCCCGACTGTCTGGAGGTGAGCACGCGGTGGGCGCTGGATCGGGAGCTTC AGGAGAAGTATGTGCTGGAGGCTGAGTGCGCAGTGGCAGGCCCTGGAGCCAACAAGGAGAAGGTGGCCGT GTCCTTCCCGGTGACGGTGTATGATGAAGACGACTCCCCGCCCACCTTCTCCGGAGGTGTGGGCACCGCC AGTGCTGTGGTGGAGTTTAAGCGGAAGGAGGGCACTGTGGTAGCCACTCTGCAGGTGTTTGATGCAGATG TGGTGCCAGCATCTGGGGAGCTGGTGAGGCGGTACACAAGCACACTACTCTCAGGGGATTCCTGGGCCCA GCAGACCTTCCGGGTGGAGCACACACCCAACGAGACCTTGGTCCAGTCCAACAACAACTCCGTGCGGGCA ACCATGCACAATTACAAGCTGGTTCTCAACAGGAGCCTGTCCATCTCAGAGAGCCGAGTCCTGCAGCTAG TAGTCCTGGTCAATGACTCAGACTTCCAGGGGCCTGGGTCAGGTGTTCTCTTCCTCCATTTCAACGTGTC TGTGCTGCCTGTCACCCTGAACCTACCCATGGCCTACTCCTTCCCAGTGAATAGGAGAGCCCGCCGTTAT GCCCAGATTGGGAAAGTTTGCGTGGAGAACTGCCAGGAGTTCAGCGGTGTCTCCATCCAGTACAAGCTGC AGCCCTCCAGCACCAACTGCAGTGCCCTAGGTGTGGTCACCTCAACAGAAGACACCTCAGGGACCCTATA TGTAAATGACACGGAGGCCCTGCGGCGACCTGAGTGTACCGAGCTTCAGTACACAGTGGTAGCCACTGAC CGGCAGACCCGCAGGCAGACCCAGGCTTCGTTAGTCGTCACAGTGGAGGGGACATACATTGCAGAAGAAG TGGGCTGCCCCAAGTCCTGTGCAGTAAACAAGAGGCGACCTGAGTGTGAGGAGTGTGGTGGCCTGGGTTC TCCAACTGGCAGATGTGAGTGGCGTCAGGGAGATGGTAAAGGGATCACCAGGAACTTCTCCACCTGTTCT CCTAGCACCAGGACCTGTCCTGATGGCCACTGTGATGCTCTGGAGAGCCGGGATATCAACATTTGCCCCC AGGACTGTCTCCGTGGCCCCATTGTTGGCGGGCATGAGCGAGGGGAGCGCCAGGGGATTAAAGCCGGCTA TGGCATCTGCAACTGTTTCCCTGATGAGAAGAAGTGCTTCTGCGAGCCAGAGGACAGCCAGGGCCCATTG TGCGATGAGCTGTGCCGTACGGTCATCACAGCCGCTGTCCTCTTCTCCTTCATAATCTCTGTCCTGCTGT CCACCTTCTGCATCCACCGCTACCACAAGCATGCGCACAAGCCACCCATCGCGTCAGCCGAAATGACCTT CTGCCGGCCGGCCCAGGGCTTCCCAATCAGCTATTCTTCCTCGGGCACCCGCCGGCCCTCACTGGACTCC ATGGAGAACCAGGTCTCTGTGGACTCTTTCAAGATCCCGGAGGATCCGAAGTGGGAATTTCCTCGGAAGA ACTTAGTTCTTGGGAAAACCCTGGGAGAAGGCGAGTTTGGAAAAGTAGTCAAGGCCACAGCCTTCCGTCT GAAAGGCCGGGCAGGATACACCACAGTGGCTGTGAAAATGCTGAAAGAAAACGCCTCCCAGAGTGAACTA CGAGACCTGCTCTCTGAGTTCAACCTTCTGAAACAAGTCAACCATCCACATGTCATCAAGTTGTACGGGG CTTGCAGCCAGGATGGGCCACTTCTTCTCATTGTGGAGTATGCAAAGTATGGATCCCTGCGGGGGTTCCT GCGGGACAGCCGCAAGATCGGGCCTGCCTATGTGAGCAGTGGAGGCAGCCGCAATTCCAGCTCCCTGGAC CACCCAGACGAAAGGGTGCTGACCATGGGCGACCTCATCTCCTTCGCCTGGCAGATCTCGAGGGGTATGC AGTACTTGGCTGAAATGAAGCTCGTACATCGAGACTTAGCTGCCAGAAACATCTTGGTGGCAGAGGGACG GAAGATGAAGATCTCTGACTTTGGGCTGTCCCGAGATGTTTATGAAGAAGATTCCTATGTGAAGAAAAGC AAGGGCCGGATTCCCGTCAAATGGATGGCAATCGAGTCTCTCTTCGATCACATCTATACCACTCAAAGTG ATGTGTGGTCCTTTGGAGTGCTGCTATGGGAGATTGTGACCCTGGGAGGCAACCCCTACCCTGGAATTCC TCCTGAACGACTCTTCAACCTTCTGAAGACAGGCCACAGGATGGAGAGGCCAGACAACTGCAGCGAGGAA ATGTACCGCCTGATGCTGCAGTGCTGGAAGCAGGAGCCGGACAAGAGGCCAGTATTTGCTGACATCAGCA AGGATCTGGAGAAGATGATGGTCAAAAGCAGAGACTACTTGGACCTGGCTGCATCCACCCCTTCGGACTC ACTGCTCTATGACGATGGGCTCTCGGAAGAGGAGACGCCCCTGGTGGACTGTAACAGTGCTCCCCTCCCG CGCTCCCTCCCTTCCACATGGATTGAAAACAAACTCTATGGCATGTCAGACCCGAACTGGCCTGGAGAGA GTCCTGTACCACTCACGAGAGCCGATGGCACTAGCACTGGGTTCCCAAGATATGCAAATGATAGTGTATA TGCTAACTGGATGGTTTCACCCTCAGCGGCAAAATTAATGGACACATTTGATAGT AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RR200483 representing NM_012643
Red=Cloning site Green=Tags(s) MAKARSGAAGLGLKLFLLLPLLGEAPLGLYFSRDAYWERLYVDQPAGTPLLYVHALRDAPGEVPSFRLGQ YLYGVYRTRLHENDWIHIDSGTGLLYLNQSLDHSSWEQLSIRNGGFPLLTVFLQVFLGSTAQREGECHWP GCARVYFSFINDTFPNCSSFKARDLCTPETGVSFRIRENRPPGTFYQFRMLPVQFLCPNISVKYKLLEGD GLPFRCDPDCLEVSTRWALDRELQEKYVLEAECAVAGPGANKEKVAVSFPVTVYDEDDSPPTFSGGVGTA SAVVEFKRKEGTVVATLQVFDADVVPASGELVRRYTSTLLSGDSWAQQTFRVEHTPNETLVQSNNNSVRA TMHNYKLVLNRSLSISESRVLQLVVLVNDSDFQGPGSGVLFLHFNVSVLPVTLNLPMAYSFPVNRRARRY AQIGKVCVENCQEFSGVSIQYKLQPSSTNCSALGVVTSTEDTSGTLYVNDTEALRRPECTELQYTVVATD RQTRRQTQASLVVTVEGTYIAEEVGCPKSCAVNKRRPECEECGGLGSPTGRCEWRQGDGKGITRNFSTCS PSTRTCPDGHCDALESRDINICPQDCLRGPIVGGHERGERQGIKAGYGICNCFPDEKKCFCEPEDSQGPL CDELCRTVITAAVLFSFIISVLLSTFCIHRYHKHAHKPPIASAEMTFCRPAQGFPISYSSSGTRRPSLDS MENQVSVDSFKIPEDPKWEFPRKNLVLGKTLGEGEFGKVVKATAFRLKGRAGYTTVAVKMLKENASQSEL RDLLSEFNLLKQVNHPHVIKLYGACSQDGPLLLIVEYAKYGSLRGFLRDSRKIGPAYVSSGGSRNSSSLD HPDERVLTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRDVYEEDSYVKKS KGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPERLFNLLKTGHRMERPDNCSEE MYRLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYLDLAASTPSDSLLYDDGLSEEETPLVDCNSAPLP RSLPSTWIENKLYGMSDPNWPGESPVPLTRADGTSTGFPRYANDSVYANWMVSPSAAKLMDTFDS SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_012643 |
ORF Size | 3345 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_012643.2, NP_036775.2 |
RefSeq Size | 5478 bp |
RefSeq ORF | 3348 bp |
Locus ID | 24716 |
UniProt ID | G3V9H8 |
MW | 124.2 kDa |
Gene Summary | may play a role in excretory system and enteric nervous system development; human homolog is associated with Hirschsprung disease and Multiple Endocrine Neoplasia type 2 [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN200483 | Ret (untagged ORF) - Rat ret proto-oncogene (Ret), transcript variant 1, (10 ug) |
CNY 8,552.00 |
|
RR200483L3 | Lenti ORF clone of Ret (Myc-DDK-tagged ORF) - Rat ret proto-oncogene (Ret), transcript variant 1, (10 ug) |
CNY 19,480.00 |
|
RR200483L4 | Lenti ORF clone of Ret (mGFP-tagged ORF) - Rat ret proto-oncogene (Ret), transcript variant 1, (10 ug) |
CNY 21,470.00 |