Selenop (NM_019192) Rat Tagged ORF Clone
CAT#: RR200391
- TrueORF®
Sepp1 (Myc-DDK-tagged ORF) - Rat selenoprotein P, plasma, 1 (Sepp1), transcript variant 1, (10 ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below)
"NM_019192" in other vectors (1)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Untagged ORF Clone |
Synonyms | Sepp1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR200391 representing NM_019192
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGAGAAGCCTAGGGCTTGCCCTGGCTCTCTGTCTCCTCCCCTATGGAGGAGCAGAGAGCCAAGGCC AAAGCCCTGCTTGTAAGCAAGCTCCACCCTGGAACATAGGAGATCAAAATCCAATGCTAAACTCCGAGGG CACAGTGACAGTGGTTGCTCTTCTTCAAGCCAGCTGATACCTGTGCCTTCTGCAGGCATCCAGATTGGAA GACCTGCGAATAAAACTAGAGAACCAAGGATATTTTAACATCTCCTATATTGTTGTTAATCATCAAGGAT CTCCTTCCCAATTAAAACATGCACATCTTAAAAAGCAGGTGTCAGATCACATTGCTGTTTACAGACAAGA TGAACATCAAACAGATGTCTGGACACTCTTAAATGGAAACAAAGATGACTTCCTCATATATGACAGATGT GGCCGTCTTGTGTATCACCTTGGTTTGCCCTACTCCTTCCTCACTTTCCCGTATGTTGAAGAAGCCATCA AGATCGCTTACTGTGAGAAGAGGTGTGGAAACTGCTCTTTCACGAGTCTTGAAGATGAAGCCTTCTGTAA AAACGTGTCCTCGGCTACTGCAAGTAAAACCACAGAGCCCTCAGAGGAGCATAACCACCACAAGCACCAT GACAAACATGGGCATGAGCATCTTGGGAGCAGTAAGCCTTCAGAGAATCAGCAACCAGGGGCATTAGATG TTGAGACAAGTCTTCCTCCTTCAGGCTTGCACCACCACCACCACCACCATAAGCACAAGGGCCAGCACAG GCAGGGTCACTTAGAGAGCTGAGACATGGGGGCAAGTGAAGGCTTGCAACTTTCACTTGCCCAGAGGAAG CTCTGACGAAGGGGATGCATAAACCAGCTCCTGTGTAAGTTATCTGAGGAGTCTGGGGCAGCTACCAGTA GCTGCTGCTGCCACTGCCGACACCTCATATTTGAGAAGTCAGGATCTGCAATCACTTGACAGTGTGCCGA AAACCTCCCATCCTTGTGTAGCTGACAGGGGCTTTTCGCGGAGGAGAAAGTCATTGAATCCTGTCAATGT AGATCACCTCCAGCTGCCTGACACAGTCAGCATGTAAGCCCCACAGAAGCCAGCCCCAACTGAAGCTGAA ATAATAAGACCAAGAAGTGAAAATGAAATTTGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR200391 representing NM_019192
Red=Cloning site Green=Tags(s) MWRSLGLALALCLLPYGGAESQGQSPACKQAPPWNIGDQNPMLNSEGTVTVVALLQAS*YLCLLQASRLE DLRIKLENQGYFNISYIVVNHQGSPSQLKHAHLKKQVSDHIAVYRQDEHQTDVWTLLNGNKDDFLIYDRC GRLVYHLGLPYSFLTFPYVEEAIKIAYCEKRCGNCSFTSLEDEAFCKNVSSATASKTTEPSEEHNHHKHH DKHGHEHLGSSKPSENQQPGALDVETSLPPSGLHHHHHHHKHKGQHRQGHLES*DMGASEGLQLSLAQRK L*RRGCINQLLCKLSEESGAATSSCCCHCRHLIFEKSGSAIT*QCAENLPSLCS*QGLFAEEKVIESCQC RSPPAA*HSQHVSPTEASPN*S*NNKTKK*K*NLN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_019192 |
ORF Size | 1155 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info The expression of this clone is not guaranteed due to the nature of selenoproteins. |
OTI Annotation | This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_019192.2, NP_062065.2 |
RefSeq Size | 2043 bp |
RefSeq ORF | 1158 bp |
Locus ID | 29360 |
UniProt ID | P25236 |
MW | 42.8 kDa |
Gene Summary | This gene encodes a selenoprotein that is predominantly expressed in the liver and secreted into the plasma. This selenoprotein is unique in that it contains multiple selenocysteine (Sec) residues per polypeptide (10 in rat), and accounts for most of the selenium in plasma. It has been implicated as an extracellular antioxidant, and in the transport of selenium to extra-hepatic tissues via apolipoprotein E receptor-2 (apoER2). Mice lacking this gene exhibit neurological dysfunction, suggesting its importance in normal brain function. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. The mRNA for this selenoprotein contains two SECIS elements. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2017] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN200391 | Sepp1 (untagged ORF) - Rat selenoprotein P, plasma, 1 (Sepp1), transcript variant 1, (Note, selenocysteine protein, internal stop codon, see reference data summary) |
CNY 3,990.00 |
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