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Grik1 (NM_001111114) Rat Tagged ORF Clone
CAT＃: RR200379
- TrueORF®
Grik1 (Myc-DDK-tagged ORF) - Rat glutamate receptor, ionotropic, kainate 1 (Grik1), transcript variant 3, (10 ug)
"NM_001111114" in other vectors (3)
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CNY 11,690.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GluK1; GluR5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR200379 representing NM_001111114
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGCAGCACAGTCCTTATCCAACCCGGGCTCTGGACCAGGGACACCAGCTGGACACTCCTCTATT TCCTGTGCTACATCCTCCCTCAGACCTCCCCTCAAGTGCTCAGGATCGGAGGGATTTTTGAAACTGTGGA AAATGAACCTGTTAATGTTGAAGAATTAGCTTTCAAGTTTGCAGTCACCAGTATTAACCGAAACCGAACC TTGATGCCCAATACCACATTAACCTATGACATCCAGAGAATTAATCTTTTTGATAGTTTTGAAGCCTCCC GAAGAGCATGCGACCAGCTGGCTCTCGGGGTGGCCGCACTCTTCGGCCCTTCCCACAGCTCCTCCGTCAG TGCTGTACAGTCTATTTGCAATGCTCTGGAAGTTCCACACATTCAGACTCGCTGGAAACACCCTTCCGTG GACAGCAGAGACCTATTTTATATCAACCTCTACCCGGACTATGCGGCTATCAGCAGGGCGGTCCTGGATT TGGTCCTCTATTACAACTGGAAAACAGTGACGGTGGTGTATGAAGACAGCACAGGTCTAATTCGTCTGCA AGAGCTCATCAAAGCTCCCTCCAGATACAACATTAAAATCAAAATCCGCCAGCTTCCCCCTGCGAATAAA GACGCCAAACCTCTGCTCAAGGAGATGAAGAAAAGCAAAGAGTTCTATGTGATATTTGATTGTTCGCACG AAACAGCTGCGGAAATTCTTAAGCAGATTTTGTTCATGGGCATGATGACTGAATATTATCACTACTTCTT CACAACCCTGGACTTGTTTGCTTTAGATCTGGAACTCTATAGGTACAGCGGTGTAAATATGACTGGATTT CGGTTGCTGAATATTGACAACCCTCACGTGTCATCCATCATTGAGAAGTGGTCCATGGAGAGGTTGCAGG CCCCGCCCAGACCCGAGACTGGTCTTCTGGATGGCATGATGACAACTGAAGCAGCGCTGATGTACGATGC TGTGTACATGGTAGCCATTGCGTCCCACCGTGCCTCTCAGCTGACCGTCAGCTCCCTGCAGTGCCATCGA CATAAGCCATGGCGCCTTGGACCCAGATTTATGAACCTCATCAAAGAGGCTCGGTGGGACGGCTTGACTG GGCGGATCACCTTCAATAAGACCGATGGCTTGAGAAAGGATTTTGACCTGGACATTATCAGTCTCAAAGA GGAAGGAACTGAAAAGATTGGGATTTGGAACTCCAACAGTGGGCTGAACATGACGGATGGCAACAGAGAC AGGTCCAACAATATCACGGACTCGCTGGCTAACCGCACACTCATTGTCACCACTATTCTGGAAGAGCCCT ACGTGATGTACAGGAAATCCGATAAGCCCTTGTATGGAAACGACAGGTTTGAAGGATATTGCCTGGATCT GCTGAAAGAACTGTCCAATATCCTGGGTTTTCTTTACGATGTTAAACTGGTTCCTGATGGCAAATATGGA GCACAGAATGACAAAGGGGAATGGAATGGGATGGTAAAAGAACTCATCGACCACAGAGCTGACCTGGCAG TGGCCCCTCTCACCATCACATACGTACGGGAGAAAGTCATTGACTTCTCCAAGCCCTTCATGACCCTGGG CATTAGCATCCTTTACCGGAAGCCCAATGGAACCAACCCGGGTGTCTTCTCCTTCCTCAACCCCCTATCT CCGGACATTTGGATGTACGTGCTGCTCGCCTGCCTAGGAGTCAGTTGTGTACTGTTTGTGATTGCGAGGT TCACACCCTACGAGTGGTATAACCCCCACCCATGCAACCCCGACTCAGACGTGGTGGAAAACAATTTCAC TTTGCTAAATAGTTTCTGGTTTGGAGTTGGAGCTCTCATGCAGCAAGGATCAGAGCTGATGCCCAAGGCT CTATCGACCAGAATAGTTGGAGGAATATGGTGGTTTTTCACCCTAATCATCATTTCATCCTACACGGCCA ACCTGGCTGCCTTCTTGACGGTAGAAAGAATGGAATCCCCCATCGATTCCGCAGACGATCTGGCCAAACA AACCAAGATAGAATATGGGGCAGTCAGAGATGGCTCGACGATGACCTTCTTCAAGAAATCAAAGATCTCC ACCTATGAGAAAATGTGGGCTTTCATGAGCAGTAGACAGCAGAGCGCACTGGTTAAAAACAGTGACGAGG GGATCCAAAGGGTGCTCACCACCGACTACGCACTGCTGATGGAGTCCACCAGCATTGAGTATGTGACGCA GAGGAACTGCAACCTCACTCAGATCGGGGGCCTCATAGACTCCAAAGGCTATGGAGTGGGGACGCCTATC GGCTCCCCTTACCGGGATAAAATTACGATTGCCATTCTTCAACTGCAAGAAGAAGGGAAGCTTCATATGA TGAAAGAGAAGTGGTGGAGGGGGAATGGCTGCCCTGAAGAAGACAGTAAGGAAGCCAGTGCTCTGGGAGT GGAAAATATCGGCGGCATCTTCATTGTTCTGGCTGCAGGACTCGTGCTTTCTGTGTTTGTAGCCATTGGA GAATTTTTATACAAATCACGGAAGAACAATGACGTTGAGCAGTGTCTCTCTTTCAATGCCATCATGGAAG AGCTGGGAATATCCCTCAAGAATCAGAAAAAATTAAAGAAAAAGTCAAGAACTAAGGGCAAATCTTCTTT CACAAGTATCCTTACTTGTCACCAGAGACGAACTCAGAGAAAAGAGACAGTGGCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR200379 representing NM_001111114
Red=Cloning site Green=Tags(s) MERSTVLIQPGLWTRDTSWTLLYFLCYILPQTSPQVLRIGGIFETVENEPVNVEELAFKFAVTSINRNRT LMPNTTLTYDIQRINLFDSFEASRRACDQLALGVAALFGPSHSSSVSAVQSICNALEVPHIQTRWKHPSV DSRDLFYINLYPDYAAISRAVLDLVLYYNWKTVTVVYEDSTGLIRLQELIKAPSRYNIKIKIRQLPPANK DAKPLLKEMKKSKEFYVIFDCSHETAAEILKQILFMGMMTEYYHYFFTTLDLFALDLELYRYSGVNMTGF RLLNIDNPHVSSIIEKWSMERLQAPPRPETGLLDGMMTTEAALMYDAVYMVAIASHRASQLTVSSLQCHR HKPWRLGPRFMNLIKEARWDGLTGRITFNKTDGLRKDFDLDIISLKEEGTEKIGIWNSNSGLNMTDGNRD RSNNITDSLANRTLIVTTILEEPYVMYRKSDKPLYGNDRFEGYCLDLLKELSNILGFLYDVKLVPDGKYG AQNDKGEWNGMVKELIDHRADLAVAPLTITYVREKVIDFSKPFMTLGISILYRKPNGTNPGVFSFLNPLS PDIWMYVLLACLGVSCVLFVIARFTPYEWYNPHPCNPDSDVVENNFTLLNSFWFGVGALMQQGSELMPKA LSTRIVGGIWWFFTLIIISSYTANLAAFLTVERMESPIDSADDLAKQTKIEYGAVRDGSTMTFFKKSKIS TYEKMWAFMSSRQQSALVKNSDEGIQRVLTTDYALLMESTSIEYVTQRNCNLTQIGGLIDSKGYGVGTPI GSPYRDKITIAILQLQEEGKLHMMKEKWWRGNGCPEEDSKEASALGVENIGGIFIVLAAGLVLSVFVAIG EFLYKSRKNNDVEQCLSFNAIMEELGISLKNQKKLKKKSRTKGKSSFTSILTCHQRRTQRKETVA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001111114 |
| ORF Size | 2715 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001111114.1, NP_001104584.1 |
| RefSeq Size | 3199 bp |
| RefSeq ORF | 2718 bp |
| Locus ID | 29559 |
| UniProt ID | P22756 |
| MW | 102.8 kDa |
| Gene Summary | Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to alter the properties of ion flow. Alternative splicing, resulting in transcript variants encoding different isoforms, has been noted for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN200379 | Grik1 (untagged ORF) - Rat glutamate receptor, ionotropic, kainate 1 (Grik1), transcript variant 3, (10 ug) |
CNY 11,690.00 |
|
| RR200379L3 | Lenti ORF clone of Grik1 (Myc-DDK-tagged ORF) - Rat glutamate receptor, ionotropic, kainate 1 (Grik1), transcript variant 3, (10 ug) |
CNY 13,400.00 |
|
| RR200379L4 | Lenti ORF clone of Grik1 (mGFP-tagged ORF) - Rat glutamate receptor, ionotropic, kainate 1 (Grik1), transcript variant 3, (10 ug) |
CNY 14,730.00 |
