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Epn2 (NM_001033914) Rat Tagged ORF Clone
CAT＃: RR200323
- TrueORF®
Epn2 (Myc-DDK-tagged ORF) - Rat epsin 2 (Epn2), transcript variant 2, (10 ug)
"NM_001033914" in other vectors (3)
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CNY 8,270.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR200323 representing NM_001033914
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAACTTCATCTATTAGACGGCAGATGAAAAACATTGTGAACAATTACTCAGAGGCTGAAATCAAAG TCCGGGAAGCCACCTCCAATGACCCATGGGGCCCATCCAGCTCTCTGATGACTGAGATTGCTGACCTGAC CTATAATGTGGTAGCCTTCTCGGAGATCATGAGCATGGTTTGGAAGCGGCTTAATGACCATGGCAAGAAC TGGCGACATGTATACAAGGCGCTGACACTGCTGGACTACCTTATCAAGACAGGTTCTGAGCGGGTGGCCC AGCAGTGTCGGGAGAACATCTTTGCTATACAGACTCTGAAGGACTTCCAGTACATTGACCGTGATGGCAA GGACCAGGGTATTAATGTTCGAGAGAAGTCAAAGCAACTGGTTGCTCTCCTCAAGGATGAGGAGCGGCTG AAGGTTGAGAGGGTTCAGGCTCTCAAAACCAAAGAGCGCATGGCTCAGGTGGCCACTGGTGTGGGCAGCA ACCAGATCACCTTCGGTCGAGGCTCCAGCCAGCCCAACCTTTCTACCAGCTACTCAGAGCAAGAGTATGG CAAGGCTGGGGGCTCGCCGGCGTCCTACCACGGCTCGCCAGAGGCCTCTCTGTGCCCCCAGCACCGCACA GGGGCCATGCTGGGTCAGAGTGAGGAGCTGCAGCCACTGAGCCAGCGCCACCCCTGCCTGCCGCACCTGG GCCTAGCCTCCCACCCAAATGGCGACTGGGCCCAGCCCTGCCTCACTTGTGACCGCGCAGCCCGAGCTAC TTCCCCACGAGTGTCCTCTGAGTTGGAGCAGGCCCGGCCACAGACCAGCGGAGAAGAGGAGCTGCAGCTG CAACTGGCACTTGCCATGAGCAGAGAGGTTGCAGAACAGGAAGAACGCCTCAGGCGGGGTGATGACCTCA GGTTGCAGATGGCTCTGGAAGAAAGCCGGAGAGACACAGTAAAAGTTCCAAAAAAGAAAGAGGTGAAAGC TTGCTGCAAGCCAGGCTCCCACTCGCAGCAGACTACCTTGTTGGATTTAATGGATGCCCTCCCCAGCTCA GGCCCTGTTGCACAGAAAACTGAGCCGTGGAGTACGGGAACCCCTGCCAACCAGACCAACCCCTGGGGTG GAACCGTGGCACCTGCGAACATTTCTGACCCCTGGCCTTCATTTGGTACCAAGCCAGCTGCCTCTGTGGA CCCCTGGGGAGTACCTACCACAGCCAGCATACAGTCTGTCCCCAAGAACTCAGACCCTTGGGCAGCCTCA CAGCAGCCTGCCTCCGATGCTGGAAAAACAGCTGATGCCTGGGGGGCTGCCAAGCCTAGTCCTGCCTCAG GGTCCTTTGAGCTCTTCAGTAATTTCAACGGTACAGTTAAAGACGATTTTTCTGAATTCGACAACCTTCG AACTTCAAAAAAACCAGCTGAGTCAGGGGCCTCAGTACCACCCCAGGACAGCAGAACCACGAGCCCTGAC CTCTTTGAGTCTCAATCCTTGACTTCTGCCTCGAGCAAGCCTAGCAGTGCTCGGAAAACACCTGAGTCCT TCCTGGGCCCCAATGCAGCACTGGTGAACCTGGACTCACTGGTGACTAAGCCTAATCCACCAGCTCAGTC CCTCAATCCCTTCCTGGCACCAGGTGCTGCTGCTCCAGCTCCTGTCAATCCCTTTCAGGTCAACCAGCCC CAGCCACTGACACTGAACCAGCTTCGGGGAAGCCCTGTCCTGGGAAGCAGTGCGTCCTTTGGGTCTGGTC CAGGGGTGGAGACGGTGGCTCCCATGCCCTCTGTAGCTCCACACTCAGCACTGGGGGCCACTGGCTCCTC ATTGACACCACTAGGCCCTACAGCAATGAACATGGTAGGCAGTGTGGGTATTCCCCCATCAGCAGCTCAG CCAGCGGGCACAACCAACCCTTTCCTTCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR200323 representing NM_001033914
Red=Cloning site Green=Tags(s) MTTSSIRRQMKNIVNNYSEAEIKVREATSNDPWGPSSSLMTEIADLTYNVVAFSEIMSMVWKRLNDHGKN WRHVYKALTLLDYLIKTGSERVAQQCRENIFAIQTLKDFQYIDRDGKDQGINVREKSKQLVALLKDEERL KVERVQALKTKERMAQVATGVGSNQITFGRGSSQPNLSTSYSEQEYGKAGGSPASYHGSPEASLCPQHRT GAMLGQSEELQPLSQRHPCLPHLGLASHPNGDWAQPCLTCDRAARATSPRVSSELEQARPQTSGEEELQL QLALAMSREVAEQEERLRRGDDLRLQMALEESRRDTVKVPKKKEVKACCKPGSHSQQTTLLDLMDALPSS GPVAQKTEPWSTGTPANQTNPWGGTVAPANISDPWPSFGTKPAASVDPWGVPTTASIQSVPKNSDPWAAS QQPASDAGKTADAWGAAKPSPASGSFELFSNFNGTVKDDFSEFDNLRTSKKPAESGASVPPQDSRTTSPD LFESQSLTSASSKPSSARKTPESFLGPNAALVNLDSLVTKPNPPAQSLNPFLAPGAAAPAPVNPFQVNQP QPLTLNQLRGSPVLGSSASFGSGPGVETVAPMPSVAPHSALGATGSSLTPLGPTAMNMVGSVGIPPSAAQ PAGTTNPFLL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001033914 |
| ORF Size | 1920 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001033914.2, NP_001029086.1 |
| RefSeq Size | 4344 bp |
| RefSeq ORF | 1923 bp |
| Locus ID | 60443 |
| MW | 68.5 kDa |
| Gene Summary | The protein encoded by this gene is a member of the epsin protein family. Epsin proteins are endocytic adaptors that function in the formation of clatherin-coated vesicles. Epsins contain a highly conserved N-terminal homology domain that binds phosphatidylinositol 4,5-bisphosphate in the plasma membrane, two or three ubiquitin interacting motifs, two clathrin-binding motifs, a cluster of aspartate-proline-tryptophan/phenylalanine repeats, and two or three asparagine-proline-phenylalanine tripeptide repeats at the C-terminus. In mouse, simultaneous knockout of this gene and its paralog results in embryonic arrest due to disruption of Notch signaling, suggesting a role as a specialized endocytic adaptor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN200323 | Epn2 (untagged ORF) - Rat epsin 2 (Epn2), transcript variant 2, (10 ug) |
CNY 8,270.00 |
|
| RR200323L3 | Lenti ORF clone of Epn2 (Myc-DDK-tagged ORF) - Rat epsin 2 (Epn2), transcript variant 2, (10 ug) |
CNY 10,070.00 |
|
| RR200323L4 | Lenti ORF clone of Epn2 (mGFP-tagged ORF) - Rat epsin 2 (Epn2), transcript variant 2, (10 ug) |
CNY 10,930.00 |
