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Gpsm1 (NM_144745) Rat Tagged ORF Clone
CAT＃: RR200162
- TrueORF®
Gpsm1 (Myc-DDK-tagged ORF) - Rat G-protein signaling modulator 1 (AGS3-like, C. elegans) (Gpsm1), transcript variant 1, (10 ug)
"NM_144745" in other vectors (3)
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CNY 8,650.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Ags3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR200162 representing NM_144745
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAGCCCGGCCCCGCCCGCGGCCGAGGAGCTCCCGGGCCCAGCCGCCAGGCGCCTCTACTCCAGGA TGGAGGCCTCCTGTCTGGAGCTGGCACTGGAAGGTGAGCGGCTGTGCAAGGCGGGGGACTTCAAGGCAGG TGTGGCCTTCTTTGAGGCTGCCGTGCAGGTGGGCACTGAGGACCTGAAGACACTGAGTGCTATCTACAGC CAGCTGGGCAATGCCTACTTCTACCTGAAGGAGTATGCCAGGGCCCTGCAGTTCCACAAACATGACTTGC TGCTGGCACGGACCATTGGCGACCGCATGGGGGAAGCCAAGGCCAGTGGGAACCTGGGCAACACACTCAA GGTCCTGGGCCGATTCGATGAGGCAATTGTCTGTTGCCAGCGACACTTGGACATTGCCCAGGAGCAGGGG GACAAGGTTGGGGAAGCGAGAGCACTCTACAACATTGGAAATGTGTACCATGCCAAGGGCAAACAGCTAT CCTGGAACGCTGCACAGGACCCTGGGCACCTGCCACCTGATGTCCGTGAGACACTGCACAGGGCCTCTGA GTTCTATGAGAGGAACCTGTCCTTGGTGAAGGAGCTAGGCGATCGGGCAGCCCAGGGCAGAGCCTATGGC AACCTGGGTAACACCCACTACCTGCTGGGGAACTTCACAGAAGCTACAACCTTCCACAAAGAGCGCCTGG CCATTGCTAAGGAGTTTGGAGACAAGGCAGCTGAGAGGAGAGCCTACAGCAACTTGGGCAATGCTCACAT CTTCCTGGGGCGCTTTGATGTGGCTGCTGAACATTACAAGAAGACGCTGCAGCTGTCTCGGCAGCTCCGG GACCAGGCCGTGGAAGCACAGGCTTGCTACAGTCTGGGGAACACCTACACACTGCTGCAGGACTACGAGC GGGCTGCTGAGTACCACCTGCGGCACCTTGTCATTGCCCAGGAGCTGGCTGATAGGGTGGGAGAGGGCCG AGCTTGCTGGAGCCTGGGGAACGCCTACGTGTCCATGGGGAGCCCCGCACAGGCCTTGACCTTTGCCAAA AAACATCTGCAGATCTCCCAGGAGATTGGAGACCGAAATGGAGAGCTCACGGCCCGCATGAATATTGCTC ACCTGCAGCTGGCACTGGGCCGGCTGACTAGCCCTGCGGCGGCAGAGAAGCCAGATCTGGCTGGCTATGA GGCACAAGGAGCAAGACCCAAAAGGACACAGAGGCTGAGTGCGGAGACCTGGGACCTGCTGCGGCTCCCC CTGGACCGGGAGCAAAATGGAGAGACCCACCACACAGGGGACTGGAGGGGTCCAAGCAGGGACTCGCTTC CTCTCCCCATGAGGAGCAGGAAGTACCAGGAAGGTCCAGATGCCATTGAGAGGAGACCCCGGGAGGGTAG CCACTCTCCACTGGACAGTGCTGATGTAAGGGTACAGGTGCCTCGCACGGGTATCCCCAGGGCCCCGTCC TCTGACGAGGAGTGTTTCTTCGATCTGCTGAGTAAGTTCCAGAGCAGTCGTATGGATGACCAACGCTGTC CCCTGGAGGAAGGCCAGGCTGGGGCTGCTGAGGCTACAGCTGCCCCAACCCTGGAGGAGAGAGCGGCTCA GCCCTCTGTAACAGCTTCACCACAGACTGAAGAGTTCTTTGACCTCATTGCCAGCTCCCAGAGCCGCCGG CTGGATGACCAGAGGGCTAGCGTAGGCAGCCTGCCTGGGCTTCGCATCACCCTCAACAACGTGGGGCACC TCCGAGGCGACGGGGACCCCCAGGAGCCAGGGGATGAGTTTTTCAACATGCTTATCAAATACCAGTCCTC CAGGATTGATGACCAGCGCTGTCCACCCCCTGATGTGCTGCCCCGAGGCCCCACCATGCCTGATGAGGAT TTCTTCAGCCTTATCCAGAGGGTGCAGGCTAAGCGGATGGATGAGCAGCGTGTGGACCTGGCTGGGAGTC CAGACCAAGAGGCCAGTGGGCTGCCTGATCCCCGGCAGCAATGTCCGCCAGGTGCCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR200162 representing NM_144745
Red=Cloning site Green=Tags(s) MASPAPPAAEELPGPAARRLYSRMEASCLELALEGERLCKAGDFKAGVAFFEAAVQVGTEDLKTLSAIYS QLGNAYFYLKEYARALQFHKHDLLLARTIGDRMGEAKASGNLGNTLKVLGRFDEAIVCCQRHLDIAQEQG DKVGEARALYNIGNVYHAKGKQLSWNAAQDPGHLPPDVRETLHRASEFYERNLSLVKELGDRAAQGRAYG NLGNTHYLLGNFTEATTFHKERLAIAKEFGDKAAERRAYSNLGNAHIFLGRFDVAAEHYKKTLQLSRQLR DQAVEAQACYSLGNTYTLLQDYERAAEYHLRHLVIAQELADRVGEGRACWSLGNAYVSMGSPAQALTFAK KHLQISQEIGDRNGELTARMNIAHLQLALGRLTSPAAAEKPDLAGYEAQGARPKRTQRLSAETWDLLRLP LDREQNGETHHTGDWRGPSRDSLPLPMRSRKYQEGPDAIERRPREGSHSPLDSADVRVQVPRTGIPRAPS SDEECFFDLLSKFQSSRMDDQRCPLEEGQAGAAEATAAPTLEERAAQPSVTASPQTEEFFDLIASSQSRR LDDQRASVGSLPGLRITLNNVGHLRGDGDPQEPGDEFFNMLIKYQSSRIDDQRCPPPDVLPRGPTMPDED FFSLIQRVQAKRMDEQRVDLAGSPDQEASGLPDPRQQCPPGAS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_144745 |
| ORF Size | 2019 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_144745.2, NP_653346.2 |
| RefSeq Size | 3425 bp |
| RefSeq ORF | 2022 bp |
| Locus ID | 246254 |
| UniProt ID | Q9R080 |
| MW | 74.4 kDa |
| Gene Summary | Guanine nucleotide dissociation inhibitor (GDI) which functions as a receptor-independent activator of heterotrimeric G-protein signaling. Keeps G(i/o) alpha subunit in its GDP-bound form thus uncoupling heterotrimeric G-proteins signaling from G protein-coupled receptors. Controls spindle orientation and asymmetric cell fate of cerebral cortical progenitors. May also be involved in macroautophagy in intestinal cells. May play a role in drug addiction.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN200162 | Gpsm1 (untagged ORF) - Rat G-protein signaling modulator 1 (AGS3-like, C. elegans) (Gpsm1), transcript variant 1, (10 ug) |
CNY 8,650.00 |
|
| RR200162L3 | Lenti ORF clone of Gpsm1 (Myc-DDK-tagged ORF) - Rat G-protein signaling modulator 1 (AGS3-like, C. elegans) (Gpsm1), transcript variant 1, (10 ug) |
CNY 10,450.00 |
|
| RR200162L4 | Lenti ORF clone of Gpsm1 (mGFP-tagged ORF) - Rat G-protein signaling modulator 1 (AGS3-like, C. elegans) (Gpsm1), transcript variant 1, (10 ug) |
CNY 11,590.00 |
