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Irag1 (NM_001105211) Rat Tagged ORF Clone
CAT＃: RR200093
- TrueORF®
Mrvi1 (Myc-DDK-tagged ORF) - Rat MRV integration site 1 homolog (mouse) (Mrvi1), transcript variant 1, (10 ug)
"NM_001105211" in other vectors (3)
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CNY 11,880.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Mrvi1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR200093 representing NM_001105211
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAAAACCAAACAGCTGATTTGTGGCTCTTGGATCCTGGAAAGGGAACCCAGCTTGGAACAGGGATGG GAAGGAGTTTGACTTGTCCTTTTGGCGTCTCCCCAGCCTGCGGAGCCCAAGCCTCTTGGAGCATCTTTGG GGTTGTCACAGCAGAGGTTCCAGGCACGCACAGCCACTCCAACCAGGCGGCTGCCATGCCCCACATTCCT GAGGATGAGGAGCCCCCCGGAGAGCCACAGGCAGCCCAGACCCAAGACTCTCCCTCTGCAGGACCGTTTT GCAGCCCTCCCACAATCATCCTGACGGGGGATGCCAGTTCACCTGGAGGAGAGACTGACACAAATCTGGT CAAGAGGGCTCCCAGCCCCCACCGCAGGCTTTCCCACCGACACCTGAAGGTCTCCACTGCCTCGCTGACT TCTGTGGACCCCTTGGGGCACGTCATTGACCTGGTAAATGACCAGCTGCCGGACATCAGCATCTCAGAAG AGGACAAGAAGAAAAACCTGGCACTTCTAGAAGAAGCCAAGTTGGTTAGCGAGCGGTTCCTGACCCGCCG CGGGAGGAGATCAAGGAGCAGCCTCGGAGACGCCCCATCAGCTGTTTCTCCAAACCTCAGCTCTGGAGCT TCTCCTGCATCCTCTAGGAGTTGCTCGTTCACCATCTCCACACCACCAGGTTTTGACACATGCAGTGGCC CACAGTCCCCTCTGCCGGGATCACCAGCACAGCAAAAGGGACATGAGGATGATGTCTTCTCGTCTCACCT CGGAGAGCCTAATGCCTCCAAAGGGCTAGCTGACCGGAAGCAGAATGACCAGAGGAAAGTGTCTCAAGGC AGACTGGCTCCTCGCTCTCCCACAGTGGAGAAATCCAAAGAACTTTCAGTAGAACAAAAGGAAAACTTCG ATCCCCTTCAGCATCTGGAGGCCACACCCATGGCTCAGACCTCTGGAGCAAGCGTCAGTGGGAAAATGGC GCTGAACAGTCCCCAGCCTGGCCCTGCGGAGATGGAGCTGGGGAGGCAGCTTCTGAAGACAGCCAGGGAA GACAACCCTCTGCCCAGAACCACAGTCCAGGGCTCAGGAGGAACGGCCTCCCCACATTCTCAGGGACAGG GCTCTGCTGGAGAGCTTATGGGGCCCAAGGCTGGCTCCAAGGCTGAGCCGAGGCCTCCTGTGTCCCGGCC CCCTCTGATTCGGGGGGTCTCCTGGGACAGCAGCCCTGAAGAACCTGGTCCCCTGCTGCAAAAGGTGCTC GCTAAGCTGCCTCTGGCAGAGGAAGAAAAGCGGTTTCCAGGCAAAGCCAAGCCAGCCAAGCCACCGGGGC TCAAAGACTTCCAGATACAAGTACAGCCGGTGCGCATGCAGAAGCTGACCAAGCTTCGGGAGGAACACAT CCTGATGAGAAACCAGAACCTGGTGGGGTTCAAGCTCCCTGAACTGAGTGAAGCTGCAGAGCAAGACAAA GGAGTCTCATCTGAGCTTGCCCCAGCAGCTGAGGAAGAAGAATCAAAGAGTGGTCTGGATGTGATGCCCA ACATTTCTGACATATTGCTACGTAAACTTAGGGTTCACAAGTCACTCACTGGAAGCGCCCCTCCGCTCAC AGAAAAGGAAGTCGAGAACGTGTTTGTACAACTGTCCTTGGCCTTTAGAAATGACAGCTACACCTTGGAG TCTAGGATTAACCAGGCTGAAAGGGAACGCAACTTGACGGAAGAGAACACCGAGAAGGAACTGGAAAACT TCAAAGCCTCTATTACGTCCTCAGCATCCATCTGGTACCACTGCGAGCACCGGGAGACCTACCAGAAGCT TCTGGAAGACATCGCAGTCTTGCATCGTCTGGCTGCACGCCTCTCCAGCCGGGCTGAAGTGGTGGGTGCT GTGCGCCAGGAAAAGCGCATGTCAAAGGCCACGGAAGTGATGATGCAGTATGTGGAGAATCTGAAGAGAA CGTACGAGAAGGACCATGCTGAGCTCATGGAATTTAAGAAACTCGCAAATCAGAATTCTAGTCGCAGCTG TGGGCCCTCTGAAGACGGGGTTCCTCGCACGGCGCGATCTATGTCCCTCACGATGGGAAAGAACATGCCC CGTCGGAGGGTCAGCGTTGCTGTGGTTCCCAAGTTTAATGCCCTGAACCTGCCTGGCCAAGCCCCCAGCT CATCACCCATGCCCTCTCTCCCAGCTCTGTCTGAATCGACCAACGGGAGAAGCAGCATCTCCACCTCTCC AGTGCTGCCTGCACTTTTGGAAAATGGAAAGACAAATGCAGAGGCTAACTGTGAAGTGTGCGCCCCTGTG CCACTCCCAAGCTACCCGGAGGAGACGAGCCAAGAGGCCAAGGCCAGAGCAGAGGAAGAAGCCTACAACA AAGGATACCAGGAAGGGGTAAAGAAGACCGAAGAACTTCAAGACCTGAAGGAGGAGGAAGAGGAACAGAA GGCAGAGAGTCCTCAGGAACCAGAAGAGGTCGAAGAAACCCAGGAAGACGAGAAGGACCAGAGAAGCAGC AAACTTGAAGAACTGGTCCACTTCCTACAAGTCATGTATCCCAAATTGTGTCAACACTGGCAAGTGATCT GGATGATGGCCGCTGTGATGCTGGTCTTGAGTGTTGTGCTTGGGCTCTACAGTTCCTATAACTCCTGCAC GGAGCAGGCCGACGGGCCCCCTGGGAGATCCACCTGCTCTGCAGCCCAGCGGGACTCCTGGTGGAGCTCG GGACTCCAGCAAGAGCTGCCAGCAGAGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR200093 representing NM_001105211
Red=Cloning site Green=Tags(s) MQNQTADLWLLDPGKGTQLGTGMGRSLTCPFGVSPACGAQASWSIFGVVTAEVPGTHSHSNQAAAMPHIP EDEEPPGEPQAAQTQDSPSAGPFCSPPTIILTGDASSPGGETDTNLVKRAPSPHRRLSHRHLKVSTASLT SVDPLGHVIDLVNDQLPDISISEEDKKKNLALLEEAKLVSERFLTRRGRRSRSSLGDAPSAVSPNLSSGA SPASSRSCSFTISTPPGFDTCSGPQSPLPGSPAQQKGHEDDVFSSHLGEPNASKGLADRKQNDQRKVSQG RLAPRSPTVEKSKELSVEQKENFDPLQHLEATPMAQTSGASVSGKMALNSPQPGPAEMELGRQLLKTARE DNPLPRTTVQGSGGTASPHSQGQGSAGELMGPKAGSKAEPRPPVSRPPLIRGVSWDSSPEEPGPLLQKVL AKLPLAEEEKRFPGKAKPAKPPGLKDFQIQVQPVRMQKLTKLREEHILMRNQNLVGFKLPELSEAAEQDK GVSSELAPAAEEEESKSGLDVMPNISDILLRKLRVHKSLTGSAPPLTEKEVENVFVQLSLAFRNDSYTLE SRINQAERERNLTEENTEKELENFKASITSSASIWYHCEHRETYQKLLEDIAVLHRLAARLSSRAEVVGA VRQEKRMSKATEVMMQYVENLKRTYEKDHAELMEFKKLANQNSSRSCGPSEDGVPRTARSMSLTMGKNMP RRRVSVAVVPKFNALNLPGQAPSSSPMPSLPALSESTNGRSSISTSPVLPALLENGKTNAEANCEVCAPV PLPSYPEETSQEAKARAEEEAYNKGYQEGVKKTEELQDLKEEEEEQKAESPQEPEEVEETQEDEKDQRSS KLEELVHFLQVMYPKLCQHWQVIWMMAAVMLVLSVVLGLYSSYNSCTEQADGPPGRSTCSAAQRDSWWSS GLQQELPAEQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001105211 |
| ORF Size | 2760 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001105211.1, NP_001098681.1 |
| RefSeq Size | 5935 bp |
| RefSeq ORF | 2763 bp |
| Locus ID | 308899 |
| MW | 100.2 kDa |
| Gene Summary | This gene is similar to a putative mouse tumor suppressor gene that is frequently disrupted by mouse AIDS-related virus (MRV). The encoded protein, which is found in the membrane of the endoplasmic reticulum, is similar to Jaw1, a lymphoid-restricted protein whose expression is down-regulated during lymphoid differentiation. Studies in mouse suggest that MRV integration at the homologous mouse gene induces myeloid leukemia by altering the expression of a gene important for myeloid cell growth and/or differentiation, and thus this gene may function as a myeloid leukemia tumor suppressor gene. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN200093 | Mrvi1 (untagged ORF) - Rat MRV integration site 1 homolog (mouse) (Mrvi1), transcript variant 1, (10 ug) |
CNY 11,880.00 |
|
| RR200093L3 | Lenti ORF clone of Mrvi1 (Myc-DDK-tagged ORF) - Rat MRV integration site 1 homolog (mouse) (Mrvi1), transcript variant 1, (10 ug) |
CNY 13,590.00 |
|
| RR200093L4 | Lenti ORF clone of Mrvi1 (mGFP-tagged ORF) - Rat MRV integration site 1 homolog (mouse) (Mrvi1), transcript variant 1, (10 ug) |
CNY 14,920.00 |
