Fga (NM_001008724) Rat Tagged ORF Clone

CAT#: RR200062

  • TrueORF®

Fga (Myc-DDK-tagged ORF) - Rat fibrinogen alpha chain (Fga), transcript variant 1, (10 ug)



  "NM_001008724" in other vectors (3)

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CNY 10,070.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Synonyms Ac1873; Fba5e
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>NM_001008724 ORF sequence, RR200062 may differ due to SNPs.
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCTTTCCCTGAGGGTCGCCTGCCTCATCCTGAGCTTGGCCAGCACAGTCTGGACTGCAGACACCGGC
ACCACAAGTGAATTCATAGAAGCAGGAGGAGATATTCGTGGCCCAAGAATTGTGGAGAGACAGCCTAGT
CAATGCAAGGAGACAGATTGGCCCTTCTGCTCTGATGAAGACTGGAACCACAAATGCCCTTCAGGCTGC
AGGATGAAAGGGTTGATTGATGAAGCCAATCAGGACTTTACAAACAGAATCAACAAGCTCAAAAACTCA
CTATTTGATTTTCAAAAGAACAACAAGGATTCTAATTCACTGACCAGGAATATCATGGAGTATTTGAGA
GGGGACTTCGCTAACGCCAACAACTTTGATAACACTTTCGGGCAAGTGTCAGAGGACCTGAGGCGCAGA
ATTGAGATCCTAAAGCGCAAAGTCATAGAGAAAGCGCAACAGATTCAGGTTCTGCAGAAAGACGTCCGG
GATCAGCTGATAGACATGAAGCGCCTGGAGGTGGACATTGATATCAAGATCCGCTCTTGCAAAGGATCC
TGCAGCAGGTCTGTAAGCCGTGAGATAAATCTAAAGGACTACGAAGGTCAGCAAAAGCAACTTGAACAG
GTCATTGCTAAAGAGTTGCTTCCGGCAAAAGACAGGCAGTACTTGCCAGCAATAAAAATGTCTCCAGTT
CCCGACTTGGTTCCCGGAAGTTTTAAGAGCCAGCTTCAGGAGGGGCCCCCAGAGTGGAAGGCATTAACA
GAAATGAGGCAGATGAGAATGGAGCTGGAGAGGCCCGGGAAGGATGGGGCTTCGCGAGGAGATTTACCA
GGAGATTCGCGAGGAGACTCTGCAACACGTGGACCAGGGTCGAAGATAGAAAACCCCATGACCCCTGGA
CATGGTGGGTCTGGGTATTGGCGTCCTGGGAGCTCCGGATCTGGAAGTGATGGAAATTGGGGCTCTGGG
ACAACGGGGTCTGATGACACTGGAACCTGGGGTGCAGGAAGCTCCAGACCTAGCTCAGGCTCTGGGAAC
CTTAAGCCTAGCAACCCTGACTGGGGTGAGTTTTCAGAGTTTGGAGGGAGTAGCAGCCCAGCGACAAGA
AAAGAGTATCACACAGGAAAACTGGTCACTTCTAAAGGAGATAAAGAGCTCCTCATTGGAAACGAGAAA
GTTACCTCTACTGGCACAAGCACCACACGTCGTTCATGCTCTAAAACCATTACTAAGACTGTTTTGGGT
AATGATGGTCACCGGGAAGTGGTCAAAGAAGTGGTCACTTCGGATGATGGTTCTGACTGCGGTGATGGC
ATGGACTTAGGCCTGACCCACAGTTTTAGTGGCAGACTTGACGAACTTTCCCGAATGCATCCTGAACTT
GGTTCCTTTTATGACAGCCGCTTTGGTTCACTCACAAGTAACTTCAAAGAATTTGGCAGTAAGACCTCT
GATTCTGACATCTTCACAGACATCGAGAACCCTAGCTCCCATGTACCTGAGTTTTCTTCCAGTAGTAAA
ACCTCAACTGTCAGGAAACAAGTAACCAAGAGCTATAAAATGGCAGATGAGGCAGCAAGTGAAGCTCAC
CAAGAAGGAGACACTCGAACCACCAAGAGGGGCCGAGCTCGCACAATGAGAGACTGCGATGATGTCCTT
CAAACACATCCTTCAGGTGCCCAAAATGGCATTTTCAGTATCAAGCTACCTGGATCCAGTAAGATATTT
TCTGTTTATTGCGATCAAGAGACCAGTTTGGGAGGATGGCTTTTGATCCAGCAAAGAATGGATGGATCA
CTGAATTTTAACCGGACCTGGCAAGACTACAAGAGAGGTTTCGGCAGCCTGAATGACAAGGGGGAAGGA
GAGTTCTGGCTAGGCAATGACTACCTCCACTTACTCACTCTGAGAGGCTCTGTCCTCAGGGTTGAATTA
GAGGACTGGGCTGGAAAAGAGGCTTATGCGGAGTACCACTTCCGGGTAGGCTCTGAGGCAGAGGGCTAT
GCACTGCAGGTCTCCTCCTACCAGGGTACCGCTGGAGATGCTCTGATGGAGGGCTCTGTGGAGGAGGGG
ACAGAATACACTTCACACAGCAACATGCAGTTCAGTACCTTTGACAGAGATGCAGACCAATGGGAAGAG
AACTGTGCCGAGGTCTACGGGGGAGGCTGGTGGTACAATAGCTGTCAAGCCGCCAATCTCAATGGCATT
TACTACCCTGGGGGCACCTATGACCCCAGGAACAACAGTCCCTATGAGATAGAGAACGGAGTGGTCTGG
GTTCCCTTCAGAGGAGCGGATTATTCTCTGAGGGCCGTTCGGATGAAAATCAGACCGCTGGTGGGACAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
>Peptide sequence encoded by RR200062
Blue=ORF Red=Cloning site Green=Tag(s)

MLSLRVACLILSLASTVWTADTGTTSEFIEAGGDIRGPRIVERQPSQCKETDWPFCSDEDWNHKCPSGC
RMKGLIDEANQDFTNRINKLKNSLFDFQKNNKDSNSLTRNIMEYLRGDFANANNFDNTFGQVSEDLRRR
IEILKRKVIEKAQQIQVLQKDVRDQLIDMKRLEVDIDIKIRSCKGSCSRSVSREINLKDYEGQQKQLEQ
VIAKELLPAKDRQYLPAIKMSPVPDLVPGSFKSQLQEGPPEWKALTEMRQMRMELERPGKDGASRGDLP
GDSRGDSATRGPGSKIENPMTPGHGGSGYWRPGSSGSGSDGNWGSGTTGSDDTGTWGAGSSRPSSGSGN
LKPSNPDWGEFSEFGGSSSPATRKEYHTGKLVTSKGDKELLIGNEKVTSTGTSTTRRSCSKTITKTVLG
NDGHREVVKEVVTSDDGSDCGDGMDLGLTHSFSGRLDELSRMHPELGSFYDSRFGSLTSNFKEFGSKTS
DSDIFTDIENPSSHVPEFSSSSKTSTVRKQVTKSYKMADEAASEAHQEGDTRTTKRGRARTMRDCDDVL
QTHPSGAQNGIFSIKLPGSSKIFSVYCDQETSLGGWLLIQQRMDGSLNFNRTWQDYKRGFGSLNDKGEG
EFWLGNDYLHLLTLRGSVLRVELEDWAGKEAYAEYHFRVGSEAEGYALQVSSYQGTAGDALMEGSVEEG
TEYTSHSNMQFSTFDRDADQWEENCAEVYGGGWWYNSCQAANLNGIYYPGGTYDPRNNSPYEIENGVVW
VPFRGADYSLRAVRMKIRPLVGQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001008724
ORF Size 2346 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001008724.2
RefSeq Size 2607 bp
RefSeq ORF 2349 bp
Locus ID 361969
MW 86.7 kDa
Gene Summary plays a role in blood coagulation; may be involved in liver regeneration [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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