Mki67 (NM_001271366) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217800 representing NM_001271366 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCCTCAGCTCGCCTGGTCACCATCAAACGGAGCGGCGATGACGGCGCACACTTCCCGCTGAGCC TCAGCTCCTGCCTGTTTGGAAGGAGTATTGAATGTGACATTCGTATACAGCTGCCTGTAGTGTCAAAAAA ACATTGCAAAATTGAAGTCAAAGAACAAGAGGCAATATTATATAATTTCAGTTCCGCCAATCCAACTCAA GTAAATGGCACTATTATAAATGAGCCTGTGCAGCTGAAACATGGAGACATAATAACAATCATTGACCGCT CCTTTAGGTATGAAGACGGAAGCCATGATGGAAGCAGGCCAACAGAATTCCCGAGAAAGTCACTTGGAAA GGAACCAGCACGGCGAGCCTCAAGAGATACCATCTCTGCTGACCCTGATGGGAAAGATCAAGATACCAAA GCTTCAAAAATGACTGCTTCAAGAAGATCTTTTGTCTATGCCAAGGGGCTTTCTGCAGATAGTCCTGCCT CAGATGACTCAAAGGACAGTGTTACCCAAGATTCATCAGGGCATGTAGAACAGCACGATCGCAGAAACCT AGGAGAACCCACTTCTGGGGATCTCTTTAAGAAGTTCAGGGCTACACGGAGCAGTTACGGGGACCTGAAG TCTTCTCCTGCACAGAGCCTTAGCAATAGCAACAAAAATGAATCTCCCTTCGAGAAACTTTATCAGTCAA TGAAGGAAGAGTTGGATGTAAAATCAGAGAAATCTCATAGAAAATCAGAACCCCAATTTGACCGTGCAGC AGAAGAATCGTGGGAGACAGAGCGATTGGTGTCATGCAAGGCAAGACCAAAGTCCAGTAGAAGCACCCCT GTTACAGCAACCTCTTCACCCAAAGTAGGAAAGAGCTGGACCGAGAGATGGAGAGGTGGTGTGGGGCCTG TTCAGACTCCCACAGAGACAGCTAAAATGAAGACCCCTGTGCAGAAGGCACAGCAGCTTAAGGATGAAGA CTCTCGTGTTCCTGGCAGAAGAAATTCTGTGAATCTGGATGAAAGTGAAAGTGCCCAGGCAGTTCATAAA ATAGTCACTCCTGGGAAACTGGTAACTAGAAATCAAGCTGCAGTAAAAGTTGGACATGTTGCCAGCCCTG CCAATACACCAGAACATTCCTCTTCCAAAAAGAGAAGAAGTATTCCTGCAGACATGGAGGTTCCATGTGC AGAGACACAAAAGCGGCTCTCTTTAACACAGTGCCTTGCTCCAGGTGAAAAGAAAAGTCCCAAGGATTCC TTCAGCAAGCCTGAGAAACTGGGCACAGTGGCCAAACAGACTTGCTCTGGTATAACTGGCCTTAGTTCCA TTGATATCAGCAACTGTGGTGATTCCATTAACAAGAGTGAGGGAGTGCCTTTGAAGAGAAGACGTGTATC CTTTGGGGGTCATCTAAGACCTGAATTATTTGATGAAAACTTGCCTCCTAATACTCCACTCAAAAGAGGA GAAACACCAACGAAGAGGAAGTCTCTTGGCACTCACAGTCCAGCTGGCCTAAAGAAAATCATCAAGGAAC GGCCTCCGTCTCCAGGGAAACAAGAGTCTCCTGGAGTAACTCCACCAAGGACAAATGATGAAAGACGAAG ATCAGGCAGGACTTCACTTGCTTCCAGCGGAAGCAAATTCTTATATGAGACAGACATTCCCAAGAAAGCA GGCAGGAAGAGTGGCAACCTGCCTGCGAAGAGAGCATCCATCAGCCGAAGTCAGCATGGCATTCTACAGA TGATTTGCTCCAAAAGAAGAAGTGGTGCTTCTGAAGCCAACCTGATTGTTGCAAAATCATGGGCTGATGT TGTAAAACTTGGCGTGAAACAAACGCAAATGAAAGTTGTGAAACATGTCCCTCAAAAGCAGACAAGAAAA AGACAAAGAAGACCCAGTACTCCAAAGAAACCTACAAGCAGCCTTCACGATCAATTTACTACAGGCCATG CAAACTCACCCTGTACCATTGTAATAGGGAGAGCACAGACTGAAAAGGCAAGCATGCCTGCCCGACCCTA CAAAATGCTGAACAACTTGATGCTAAACTGGAAAATGGACTTCAGTGAAGATCTGTCAGGACTAACTGAA ATGTTCAAGACTCCAGTGAAGGAGAAGCAGCAGCAGATGAGTGACGTGGGCTCCATTCTGTCTGATTCAG AGAATTTGTCTGAAAGACAGTTGCAAGGAACCAACTCAGAAGACACACCCCCACCCACCGCCTCAGAGAT TTTGGGAGAAAAAGTGCTCCTCAGTACTCAGAATGCAGCAAAGCAGCAATCCAATAAATATTCTGCAAGT TCTACCTTAAGACGGCAGAGCATCAAACATGAAAACACAGTGCAAACTCCTAAGAATGTCCATAACATTA CGCAAATTGAGAAGAAGACTCCAGCCTCTGAGACAGAGCCTCTAAAGACAGCATCAAGTATGAGCAAGTT AAGGAGATCTAGAGAGCTCAGACATACCCTTGTCAAAAGTAAGAATGAAAAAACAGAAGCAGTCCTTGCC AAGAGCATCACAGGAAGACATCTACGGAAGACATTTCAAGGACAAGAAGTAGACCAACAAGTGCAGGGCA GTGAAAACTCTTCACAAAGATGCGAGGAAAACAGTAAATTAAGTGAAGATTCAGAAAAGACATCCGCTAG GAGATCAAGTGCCAGGAAGCAGAAACTAACAAAAGACTTAGTAGGAAGTCAGATGGTCACCCAATCAGCA GACTGTGCTGAGGACCTACTTAGTCAAGAACAAGGAACAATACAAAACCTAGAGGAATCCATGTACACGC AAAACACATCAAAAAGTGATCAAGGAGTTGCAAAACAGAAAGTTAACACAGTGGTAGATGCAACCAGAGT GAAGCGCTGGTCAAAGACCCCTGTCAAAATGACACACCCTCTAGAAGGTGCAGCTGATTTCACGGAGCAC TTTGAAACACCAGATCTCAAAGATGAACCCATAGGTGATGATGAAACTAAAGTCCTTTGCAAATCCCCAC AACCCAAAACAGAGAACCTCAAGGCAAGCGCAAAGCCACGGACCAGCACATCTAGGAGGAAAGTAGATAG GAAGGAAGAACTCTCTGCCGTGACAAAGCTTATACATATGTCAGGGGAAAGTAGGCATACTCCCAAAGTT CTAAAACTTGAGCATGGGGATATCAAAGCTTTGAGGCAATCTGAAAATGAAATGCTGGCTGCAACAGTAA CTGGAAGCAAGAGGCCTTTAGAAAAAGCTAAGGAAAAGGCTCAGCCTGTGGAAGACCTGACTGGTTTCGA GGAACTCTTTCTATCACCAGTCCCTGGTGACAAAATCACAAAAATGCCCAGCAAATCTCCACACACAGAA TCAGTCAGAACTCCAGCAAGCACAAAGAGACTGTCCACGACAGGTCTCAGTAAGGTGGATGTGAGAGAAG AGCCTGCAACCCTTGGGAAAAGAACAAAGTCATCAGGCAGAGTGACAGGCACACCAGCACCAGCCCAGGA AGATAATGATACCACAACCTTCATGGAAACTCCCAAGCAGAAACTGGATTTCACAGGAAATTCAACTGGA CATAAGAGAAGGTCACAAGCATCCAAGAACAGGGCTCAGCCCCTAGAAGACCTGGATGGCTTCCAAGAAC TCTTCCAAACACCAGCTGCTGCTAGGACCCCTGTGACTGTTGATGAAAGTGCAAAGGTGTCTCTGGAATC TGTACAACCACAACTAGTCATAACCTCAGCAAGCACAAAGAGACTGTCCAAGACAGGTCTCAGTAAGGTG GATGTGAGAGAAGAGCCTTCGACCCTGGGGAAACGAACAAAGTCATCAGACACAGTGACAGGCACACCGG CACCAGTGCAGGAAGAAAATAACACCATGGCCTTTATGCAAACTCCAAAGCAGAAACTGGATTTCACAGG AAATTCAACTGGACATAAGAGAAGGTCACGAGCATCCAAGAACAGGGCTCAGCCCCTAGAAGACCTGGAT GGCTTCCAAGAACTCTTCCAAACACCAGCTGCTGCCAGTGACCCTGTGACTGTTGATGAAAGTGCAAAGG TGTCTCTGGAGTCTGTACAACCACAACTAGTCATAACCCCAGCAAGCACAAAGAGACTGTCCAAGACAGG TCTCAGTAAGGTGGATGTGAGAGAAGAGCCTTCGACCCTGGGGAAACGAACAAAGTCATCAGACACAGTG ACAGGTACACCGGCACCAGTGCAGGAAGAAAATAACACCACGGCCTTTATGCAAACTCCAAAGCAGAAAC TGGATTTCACAGGAAATTCAACTGGACATAAGAGAAGGTCACGAGCATCCAAGAACAGGGCTCAGCCCCT AGAAGACCTGGATGGCTTCCAAGAACTCTTCCAAACACCAGCTGCTGCCAGTGACCCTGTGACTGTTGAT GAAAGTGCAAAGGTGTCTCTGGAATCTGTACAACCACAACTAGTCATAACCCCAGCAAGCACAAAGAGAC TGTCCAAGACAGGTCTCAGTAAGGTGGATGTGAGAGAAGAGCCTTCGACCCTGGGGAAACGAACAAAGTC ATCAGACACAGTGACAGGCACACCGGCACCAGTGCAGGATGAAAATAACACCACGGCCTTTATGCAAACT CCAAAGCAGAAACTGGATTTCACAGGAAATTCAACTGGACATAAGAGAAGGTCACGAGCATCCAAGAACA GGGCTCAGCCCCTAGAAGACCTGGATGGCTTCCAAGAACTCTTCCAAACACCAGCTGCTGCCAGTGACCC TGTGACTGTTGATGAAAGTGCAAAGGTGTCTCTGGAATCTGTACAACCACAACTAGTCATAACCCCAGCA AGCACAAAGAGACTGTCCAAGACAGGTCTCAGTAAAGTGGATGTGAGAGAAGAGCCTTCGACCCTGGGGA AACGAACAAAGTCATCAGACACAGTGACAGGCACACCGGCACCAGTGCAGGAAGAAAATAACACCATGGC CTTTATGCAAACTCCAAAGCAGAAACTGGATTTCACAGGAAATTCAACTGGACATAAGAGAAGGTCACGA GCATCCAAGAACAGGGCTCAGCCCCTAGAAGACCTGGATGGCTTCCAAGAACTCTTCCAAACACCAGCTG CTGCCAGTGACCCTGTGACTGTTGATGAAAGTGCAAAGGTGTCTCTGGAATCTGTACAACCACAACTAGT CATAACCCCAGCAAGCACAAAGAGACTGTCCAAGACAGGTCTCAGTAAGGTGGATGTGAGAGAAGAGCCT TCGACCCTGGGGAAACGAAGAAAGTCATCAGACACAGTGACAGGCACACCGGCACCAGTGCAGGATGAAA ATAACACCATGGCCTTTATGCAAACTCCAAAGCAGAAACTGGATTTCACAGGAAATTCAACTGGACATAA GAGAAGGTCACGAGCATCCAAGAACAGGGCTCAGCCCCTAGAAGACCTGGATGGTTTCCAAGAACTCTTC CAAACACCAGCTGCTGCCAGTGACCCTGTGACTGTTGATGAAAGTGCAAAGGTGTCTCTGGAATCTGTAC AACCACAACTAGTCATAACCCCAGCAAGCACAAAGAGACTGTCCAAGACAGGTCTCAGTAAGGTGGATGT GAGAGAAGAGCCTTCGACCCTGGGGAAACGAACAAAGTCATCAGACACAGTGACAGGCACACCGGCACCA GTGCAGGATGAAAATAACACCACGGCCTTTATGCAAACTCCAAAGCAGAAACTGGATTTCACAGGAAATT CAACTGGACATAAGAGAAGGTCACGAGCATCCAAGAACAGGGCTCAGCCCCTAGAAGACCTGGATGGCTT CCAAGAACTCTTCCAAACACCAGCTGCTGCCAGTGACCCTGTGACTGTTGATGAAAGTGCAAAGGTGTCT CTGGAATCTGTACAACCACAACTAGTCATAACCCCAGCAAGCACAAAGAGACTGTCCAAGACAGGTCTCA GTAAAGTGGATGTGAGAGAAGAGCCTTCGACCCTGGGGAAACGAACAAAGTCATCAGGCAGAGTGACAGG CACACCGGCACCAGTGCAGGAAGAAAATAACACCACAACCTTCATGGAAACTCCAAAGCAGAAACTGGAG TCTTTAGGAGATTTAACAGGGCTTAGGAAACAGTCCAGAACACCTAAAGCAAAAGCCCAATATCTAGAGG ACATATCTGGTTTTCAGGAGGGTTTCCAGACACCAGACCATGCCAATGGCCCATTAGCAGTTGGCAAAAC CAAAAAAATGTCCTTTATTTCTCCACAACCAGGAAGACCAGTTAGAACCCAAAACAACAGAAAGAGTCTG CCCAAGATTAGCCTGAGACAAGTAGATGTGACGGGAGGAATTTCAGTTCTCGGGAAGCAGTCACCTGGCA GAGCCCTCACAGCACCAGTGCAGGCGGATAATGGGATCAAAGCAATTATGGAGACTCCAAAGGAAACACT GGCAACTGCAGCAGATTTAACTGGGTTTACCAGACCACAACAAACACCTAAGGAAAAAGACCAGCCCCTG GAAGATTATGGTGGCTTCCAAGAACTCTTCCAAACACCAGCTGGTGCCAGTGACTCAGTGACTGTTGAGG AAAGTAAAAAGATGCCAGTGGAATCTTTACAACCACAACCAGTCAGAACCCCAGCAAGCACAAAGAGACT GTCCAAGACAGGTCTCAGTAAGGTGGATGTGAGAGAAGAGCCTTCAACACCTGGGAAAAGAACAAAGTCA TCAGACACAGTGACAGGCACACCTGCACCAGTGCCGGAAGAAAATGACACCACACCCTTTATGCAAACTC CAAAGCAGAAACTGGAATTCACAGGAAATTCAGCAGGACATAAGAGAAGGTCACGGACACCTAAGACCAG AGCTCAGCCCCTAGAAGACCTGGATGGCTTCCAAGAACTCTTCCAAACACCAGCTGGTGCCAGTGACCCT GTGACTGTTGATGAAAGTACAAAGGTGTCTCTGGAATCTTCACAACCACTACCAGTCATAACCCCAGCAA GCACAAAGAGACTGTCCAAGACACCAGGACAGAACAAGGATTTAGTGACACTGGACAATCTTGCAAAAAT GCCAGGCAGTTCTCCACCACCAGAGCCAATAGATACTTCAGTAATCTCAGGTAGACAGCTCAGAACTCGT CTTAGGAACCTTTATGTGAAAAATGAACTGTCAGAAGACGGAATGCATCCAAAAATGTCAGGGGAAATTG TGGACTCATCTAGAGCACCAGGAGGTGAAGAGAAAGTCATTAAAGCAAGGAAGCAATGTGTAAAGCGGAA ATTGGACACAGCAGTCAATTTACCTAGCAGCAAGAGGCAAAGAATTGCAGGAGCAGAAGAGCCACAGACC CTAGAGGATCTGCCTGGCTTCCAAGAGCTCTGCCAAGCTCCATGCTTGGTAATGGACTCAGTTATTGTTG ACAAAACCACAAAGGTGCCCAGCAAATCTCCAGAACCTGTGGATACAACTTCAGAGATAAAGCCAAGAAG ACGACTCAAGAGACTGGGTGTGACTGAAGAGCCCATACCACAAAGAACGACTACAAGAGTGTTAAGGCAA ACCAGAAACACACACAAAGAGCCCATAGATAGTAGTAATGGTGTAGATGAGTTTAAGGAGTCTTCGGCAC AGAAACAAGACCCAGCCATAAGTTTAACCGGCAGGAGGAATCAACCAAGAAAGGTTAAGGAAAAGACCCA ACCCTTGGAAGAGCTGACTAGTTTCCAAGAGGAAATTGCCACAAGAATGTCTTGCAAATCTCCACAACCA GAAGAGAAGAAAACCTTAGTAGGCTTAAAGAAGCAGCTCAGAATACAACTATTCAAAGTGGGTGTGGAAG AGGAGCCCACAGCACAGAGAAAGCAACCAGCCAGGGAAACCAGGAGCAGACTCAAAGAGCCCATGGGTAA CAGGATAAATGTTGAAGAGCTTAAGAAGTCTACAGAGCAGAAAATTGACCCAGTAGCAAGTATGACTGTC AGCAAGAGGCCACGGAGGATACCCAAGGAAAAGGCACAGACCGTAGAATTGGCTGGTCTCAAAGGACCAA TCCAAACCCTAGGTCACACTGAGGAATCTGCAAGGGATAAAGAACCCACACAGATGCCCTGTAATTCTCT ACAACCAGAGCAAGTTGATAGCCTCCAACCCTCACCAAGACGTCCCAGGACAAGACATGGGAAAGTAGAG GCAGACAAGCCTTCAGCAGTAAGGAAGACAGTGCCAACATCAAGGCAAAGTGTGCGATCCCGAAAGGTCA CTGAAATTGATGACAATGGTACCAAAGCTTCAAAGGCACCTGTAAAGCAGACATTAGATACAGTAGCCAA AGAAACTGACAGCAAAAGGCAGCTAAGGACACGTAAGAATGGGGTTCAACCTCTTGAAGTCCTGGGTGAC TCCAAAGAAATAACACAAATATCAGATCACTCTGAGAAACTAAGACATGACACTAATATTCTTAAGAGCA CTCTACAGCAAAAGCCAGACTCAATGAAACCTCTGAGAAAATGCAGAAGAGTGCTGAGGACCTCTAAAGA GGACCCCAAAGAAGTGTTGATGGATACCAGTGACCTTGCAGCATTACAAAGCAAAAGCAACCTTTACCTG TCCCCCCCCAGGAAGTCTGCAAGAGATGGAAGGATTTTGAAAACCAGGACTTTGTGCTCTGTAACCCCAA AGCAGGAAGCAACAGGTGAGAAGCCTGTACATAAGAAACAAAGGGCAGCTTCTACCAAGAGGCAGGTGTC ACCTGAGCCGGTGAAGGTGAAACACCTGAGAAGTGTGTCACATAAACGTGAATCTGTGGAGGAGCACATT AGCACCATTATGCAAATAGAAGAAATGGAAGCCAAAAGAGAGAACCCTGTCACTCCAGATCAGAGCTCTA GGTACCGAAAGAAAACTGAAAAACAGCCAAGCCCCAAGTTTGATGCGTCTGCAGAGAAGGTTGGGATAAA GAAAAATGAGAAAACAATGAAGATCTCCCAGGAGACAGAGCCGCAGAATCCAGATCCTGGAACCAAGAAG TCTTCATCTCTGGGCAAAGTCAGTGGGAAAAGAACATGTTTGAGGTCTAGAGGACAGACGGAGATTCCCC AGCCTCATGAAGCAGAAGAGAAAACAAGAAAATCAGGTGCAGAAATCTCGATAAAGACCCAGCAAGAGAA AGGAGTCTCTGGAGATCCAGATGTTAGGCGTTTGAGATCCAGACAAACTAGAGTCACTTTGGACAATGAA CCTAAGCCAAGAGTAACTCGAGGGGCCAAGAAAGATGCAAAAACTCTGAAGAAGGATGAAGACATTGTAT ACACCAAGATATTAAGAACAAGAAGTCACCAGAAAAGTGAAACTGTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271366
Insert Size	9360 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271366.1, NP_001258295.1
RefSeq Size	9880 bp
RefSeq ORF	9360 bp
Locus ID	291234

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