Cenpf (NM_001100827) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Lek1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217796 representing NM_001100827 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTGGGCGTTGGAAGAGTGGAAGGAAGGTCTCCCGACCAGAGCTCTTCAGAAAATTCAAGAGCTTG AAGGACAGCTGGACAAACTGAAGAAGGAGAAACAGCAGAGGCAGTTTCAGCTGGATTCTCTCGAAGCCGC GCTGCAGAAGCAGAAGCAGAAGGTTGAAGATGGAAAAACTGAGGGTGCAGACCTGAAAAGGGAAAATCAA AGATTGATGGAGATATGTGAAAATTTGGAGAAAACACGGCAGAAGCTTTCTCACGAACTTCAAGTTAAGG AGTCACAAGTGAATTTTCAAGAAAGTCAACTGAGCTCATGCAAAAAGCAAATAGAAAAGCTGGAGCAGGA GCTTAAGCGGTGTAAATCTGAGTTTGAGAGAAGCCAACAATCGGCAGAGGTTTCCTTAAACCCATGCAGT ACACCCCAGAAACTCTTCACCACTCCACTCACACCAAGTCAGTATTATATCTGTTCCACGTATGAAGATC TAAAAGAAAAATATAATAAAGAAGTTGAAGACCGGAAAAGATTAGAGGCAGAGGTTAAAGCTTTGCATGC GAAAAAAGCGAGCCTGCCTGTTTCCCAAGCCACCATGAATCACCGGGACATTGCAAGACATCAGGCTTCA TCATCGGTATTTTCGTGGCAACAGGACAAAACCCCAAGTCGCCTTTCATCTTGTTCTCTGAGAACCCCAC TACGGAGAGATGTCTCTGCTGCTCACTTTTTGGGGGAAGAAGTGACCCCTAACAAATCAAGTGTTCAGAT AGGGAGAGGAGACTGCAGTGGCCTCCCTGACGATCCTCATTGCTCTCAGCCTTTGCATCAGGCCAAAGCC CAGAATCAAGAACTAAAAAGCAAGATGAATGAGTTAGAACTACGCCTGCGAGGACAAGAAAAGGAAATGA AAGACCAAGTGAATAAATTCCAAGAGTTACAGCTACAACTGGAGAAAACAAAAGTGGACTTAATTGAAAA AGAGAAAATTTTGAATAAAACCAGAGATGAAGTAGTGAGAACCACAGCCCAGTATGAGCAAGCAGCAGCC AAGTGTACTGCCTTGGAACAAAAGCTGAAGAATTTGACGGAGGAGTTGAGTTGTCATCGACAGAACGCAG AGAGTGCTAAACGTTCTCTGGAACAGAGAGTTAAGGAGAAAGAAAAGGAACTTCGAGAGGAGCTGTCCCG ACAACATCAATCTTTCCAAGCTCTGGACCATGAGTACACTCAGATGAAAACCAGACTTACCCAGGAGTTA CAGCAAGCCAAGCATTCGCTCAGCGTCCTCCAGCTGGAGCTGGAGAAGGTCACATCAGTGAAGCAGCAGT TAGAAAGGAGTTTGGAAGAAATTAGGCACAAGTTCAGCAGAGCAGAACAAGCTCTTCAGGCGAGTCAGCT CACAGAGAATGAGCTGAGGAGAAGCAGTGAGGAAATGAAGAAGGAAAACAGTCTCATTAGGAGTCAGTCT GAGCAAAGGACCAGGGAAGCCTGCCGCCTGGAGGACGAACTTGGTAAAGTCAAAGTGTGTTTGAGTCAGA GCCAGAGTTTTGCAGAAGAAATGAGAGCTAAGAATACCTCTCAGGAAATCATGTTACGAGATCTTCAGGA AAAACTAAATCAGCAAGAAAACTCACTAACTTTAGAAAAACTGAAACTTGCCTTAGCTGACCTGGAAGGA CAGCGAGACTGTTCTCAAGATCTTTTGAAGAAAAGGGAACATCACATTGAGCAACTGAATGATAAGTTAA ATAAGATAGAGAAGGAATTTGAAACTTTGCTGAGTGCTTTAGAATTAAAAAAGAAAGAATGTGAAGAATT GAAAGAAGAGAAAAATCAGATTTCTTGTTGGAAAAGTGAAAATGAAAAACTCATAAATCAGATAGAGTCA GAAAAAGAAATCTTACTGGGTAAAGTCAACCACTTAGAGGCCAGCCTCAAGACACAACAAATAAGTCATG ACTATAGTGAGAGAGTAAGGACACTAGAGATGGAAAGAGAAAACCTTACTGTGGAGATTAGAAACCTCCA TAGTCTTTTAGACAGCAAGATGGTGGAGATCGAGACACAGAAACAAGCCTACTTGGAACTGCAGCAGCAA TCTGAATCCTCAGACCAGAAGCATCAGAAAGAAATGGAGAATATGTGCTTGAAAGCTAATAAGCTCACTG GGCAAGTTGAAAGTTTGGAGTGTAAGCTGCAGTCACTGTCAGGGGAAGTAGAAACCAAAGACCAGCAGTA CCAAGACTTGCGTATGGAGTACGAGACAATGAGGGATTCGCTCCAGGCCAGAGGATCTTCTCTGGTGACG GATGAGAAGAATCAGAGAAGCTCTTCAGCTTTTGAAGAGCAGCCTGCAGTGAGTCATTCCTTTGCAAACC TAGTGGGGGAAAAAGGAAGCATATATTCAGAAAGGAGTGACAGCTCTGTAGATAGGGGCCAAAGTCCAGA AAATGTAGCTGTCTTACAAAGTAGAGTCACTTTACTTGAAAGTTCCTTGGAGTCTCAAAACCAGATGAAC TCAGATTTGCAAAAGCAGTGTGAAGAGTTGCTACAAATCAAAGGGGAAATTGAGGAAAACCTCATTAAAG CAGACCAGATTCATCAAAACTTTGTGGCTGAAACAAATCAACGTATTGGTAAATTGCAGGAAGATGCTGC AGTTCATCAGAATATTGTTGCTGAAACTCTAGCAACTCTTGAGAGTAGGGAAAAAGAGTTACAGCTTTTG AAAGAACAATTAGAAGCCCAGCAAACAGAGGTTCAAAAGTTAGAGAAGAATAACTGTCTTCTTGAAGGTG CTCTGAAGGAGCTACAGCTTTTAACTGACACTCTGAGCTCCGAGAAGAAAGAAATGAATTCTATCATCTC ATTAAGGAAAAAAGACATTGAAGAGTTAACCCAAGCAAATGGGGCTCTCAAAGAAGTTAATGAGGCCTTA CGGCAGGAGAAAATGAATCTTCTCCAACAGCATGAGGAGATTACAAGGTGTGTAGCAGAAGGAGAGAGAA GCATTGCAGAGTTGTCTGGTCAGTACAAGCAAGAAAGACTTTTGTTATTACAACGGTGTGAAGAAACAGA AACTGTGTTGGAAGGTCTCAGAGGAGACTACAAAGCAGCACAAGAAAACAATGCTAAGTTAGAATGCATG CTCAGTGAGTGCACTGCTCTTTGTGAGAATAAAAAAAATGAACTAGAGCAGTTAAAGGAAACATTTGCAA AGGAACAGCAAGAATTCTTAACAAAATTAGCTTTCACTGAAGAGCAAAACAGGAAGTTAGTGCTAGAGTT GGAGACAGAGCCACAAACTGTGAGATCTGAGATTACAAACATCAACAAGCATCCCGTGAGTGAGACTGAT GCCTTAGGGCAAGAAAGCTGGTCTTCAAAGGAAGAGCAAAAGGAGAAGCAAAAGGAAGTCAGCAACTTAA CACCTGAGAACGAGCAATTGATGGAGTTAACACAGACTAAACATGATTACTATCATCTTGAAGTAGAGCC AGTTGAGAACTCTGTAAAAGCAACAGAAGAAGAGATAAGGAAGAGTAGTTCCCAGTATCAGATGGATATT GACACTAAAGACATTTCTCTAGACAGTTATAAAGCACAGTTGGTACAACTAGAAGCTTTGATAAAAGTTA TGGAAGTAAAACTCGACCGAAGTGAGGAGGAGAAAAACAGCCTGCGTCAGGAATTACAGACAATTAGAGA AGAGCTGGGAACCAAGACTTCACAGGACACTCAGTCACAGGCGAGGGTTGGCCTTAAAGACTGTGAAGTA GAAGCAGAGGAAAAGTATGTGTCAGTGCTCCAGGAGTTGTCAACAAGTCAAAATGAGAATGTGCACTTAC AGTGCTCTCTGCAGACCGCAATGAACAAATTGAATGAACTAGGGAAAATGTGTGAAGTACTGAGAGTTGA AAAGTTACAACTAGAGTCTGAGCTGAATGACTCACGATCTGAGTGTATCACAGCAACTAGTCAAATGGCG GCAGAGGTCGAGAAGCTAGTGAATGAAATGAAAATGCTCAACCATGAGAATGCTCTGTCCCACGGTGAGC TGATGAAAGATACTGCAGATGTTGAATTTGATGATAAACCAAACCACACATCTGTGTTCTTGACTCCTTT GGACAATAGTGAACAAATGACCTCATCAAACAAAGAGGTCCGAGTACACTTTGCTGAATTACAGGAGAAA TTCTCCTGTTTACAAAGTGAACACAAAATTTTACATGATCAGCACTGTGAGGTGAGCTCTAAGATGTCAG CACTGCGTTCCTATGTTGACACATTAAAAGCTGAAAATTCTGTCTTGTCAATGAATCTGAGAACTTTGCA GGGTGACTTGGTAAAGGAGAAGGAGCCTGGAGCTGAGGATGGACATATTCTGCCACTGTCATTCTGTAGG ACAGACAGCCCTACTCTGACAAATTTTGGAGAAAATTCCTTTTATAAAGATGTTTTAGAACAAACTGGAG ACACATCTCATTTAAGTTTAGAAGGGAATGCTTCAGCAAATCCTTGTGATGTAGATGAAGTGTCTTACAG TAGTCTGGAGGAGGAGAATCTGACTGAGAAAGAAATCCCATTTGCCTCTCTGAGGACTGTTGAAGAACTT GAAATCCTGTGTCAGGTGTACTTGCAGTCCATCAAGAATCTAGAAGAGAAAATTGAGAGTCAAAGGATTA TGAAAAATAAAGAAATTGAAGAGCTTGAGCAGTTACTAAGTTCTGAGAGGAAGGAACTAAGCTGTCTTAG GAAGCAATATTTGTCAGAAAAGGAACAATGGCAGCAGAAGCTGACAAGTGTCACTTTGGAAATGGAGTCC AAGTTAGCAGAAGAGAAGCAGCAGACCAAGAATTTGTCCCTTGAACTTGAGGTAGCACGACTTCAGTTGC AGGAGCTGGACTTAAGCTCAAGGTCTTTGCTTGGCACTGACTTGGAAGGTGCTGTTCGGGGCCCAAATGA TGGTTATGCTATAAAAGAATCAGAAGTATATATCTCAGAGACTACAGAGAAAACGCCAAAGCAGGACACT AATCAGACTTGTGATAACGATGTTCAGCAGGACCTTTGTCTAGAAACTTCAGTCACTGAGACCGAGACTA CCAGGCTCACAGGAGATGGGTGTGAAGAGCAGCCTTCAAAGATCAGTTGTGAGGCACCAGCAGAGGACAG AACCCAAGACTATTCAGAATGCATTTCTGAATTGTTTTCTACTCCCAGTGTTTTAGTACCCATGGATGTT CTGGAAGATCAGGGGTCTATCCAGAATCTCCATTTGCAGAAAGATACATCAAATGAGAATTTGAGATTAC TTCCTGAAGTAGAAGACTGGGACAAAAAAGTTGAAAGTTTGCTGAATGAAATTAAGGAGGCAGATTCAAA ACTCAGTTTACAGGAACTGCAGCTAAAGATCAAGATTGCAACATGTATAGAATTGGAAAAAATAGTCAAG CACCTCAAGAAGGAAGAAACTGACTTAAGTGAAAAATTGGAATCTCTTCCGTGTAACCAGGAGGTATGTC CAAGAGTAGAAAGGTCAGATCTTGATTTTAACTTAGATATGGGAGACGATGAGTTGTTTCGTGAATCTAC TAAAGATGATGCAGCCAACACAGAAGACAATTACAAGGAGAAGTTTCTTGATATGGAAAGAGAACTGACC AGGATTAAGTCTGAGAAAGCTAATATTGAGCATCACATTGTATCTGTGAAAGCTAACTTAGAGGTAGTTC AAGCAGAGAAGCTCTGTTTAGAAAGAGACACTGAAAGTAAGCAGAAGGTTATTGTTGACCTTAAAGGAGA ACTATTTACAGTTATAAGTGAGAGAAACAGACTTCGTGAAGAACTAGATAATGTGTCAAAAGAAAGCAAA GCACTGGACCAGATGTCTAAAAAGATGAAGGAGAAAATAGAAGAGCTGGAGTCTCACCAGAGGGAGAGCC TCCATCACATTGGGGCAGTAGAGTCTGAGGTCAAGGACAAAGCAGAACTCATTCAGACTCTGTCCTTCAG TGTGGATGAGCTAACAAAAGACAAAGCTCATCTCCAGGAGCAGCTGCAGAATTTGCAGAATGACTCACAG GGATTGTCTTTGGCAATTGGTGAGCTGGAAATCCAGATTGGACAACTGAATAAAGAGAAAGAATCACTAG TCAAGGAGTCTCAAAACTTCCAGGTCAAGCTGACTGAGTCGGAATGTGAAAAGCAGACCATTTCTAAGGC CTTGGAGGTGGCACTCATGGAGAAAGGTGAGTTTGCAATGCGACTGAGCTCAACCCAGGAGGAGGTGCAT CAGCTGAGACAAGGCATTGAGAAACTGAGTGTCCGAATTGAGGCAGATGAAAAGAAGCACCTCAGTGCTG TGGCGAAACTGAAAGAAAGCCAGCGGGAAAGTGACTCATTGAAGGATAAAGTGGAAAATCTGGAGAGGGA GTTGGAGATGTCAGAAGAAAACCAAGAGCTGGCGATTCTTGATTCTGAGAATTTGAAAGCAGAGGTGGAG ACCCTCAAGGCACAAATGGATGAAATGGCCAAAAGCCTGAGAGTTTTTGAATTAGACCTTGTCAATGTTA GATCTGAAAGAGAAAATCTAGCAAAGCAGCTGCAAGAGAAACAAAGTCGAGTGTCAGAATTAGATGAACT GTGCTCTTCCTTGAGAAGTCTGTCAGAAGAGAAGGAGCAAGCAAGAGTACAGATGGAAAGAGACTCTAAA TCAGCAATGCTGATGCTTCAGACACAGTTGAAAGAGCTCTGGGAGGAAGTAGCAGCCTTGTATAATGACC AAGAAACCTTAAAGGCCCAAGAACAGAGTCTAGACCAACCAGGGGAAGAAGTGCATCTCTTGAAAAGTAG TATTCAAAAACTAAAAGTTCACATAGATGCTGACAAAAAAAAGCAGTGCCACATCCTAGAACAGCTGAAG GAAAGTAAGCATCACGCAGACTTGTTTAAGGACAGAGTTGAGAACCTTGAACAAGAATTGATGCTATCCG AGAAAAACAAAGAACACTTGATTTTCCAAGCTGAAAATTCCAAAGCAGAGATACAGACTTTAAAAACAGA AATTCAAACAATGGACCAAAACCTCCAAGATTTGGAGTTAGAACTTACTAATACAAGGTCAGAAAAGGAA AATCTCATGAAAGAACTAAAAAATGAGCAAGAGCAAATATCTAAATTAGAAACAATAAATTCTTCTATTG AAAGATTACTGACAGATAAAGAGCAAGAAAAAGTACAGGTGAAAGAGGAAGCCAGAATTACAGTGGAGAT GCTTCAAACTCAGTTAAAGGAGCTAAATGAGACAGTGGTTTCCTTGTGCAATGACCAGGAGGCCTATAAG ACCAAAGAGCAGAATCTGGGGAGCCGAGTACAAACTCTTGAACTTGAGAAGGCTCAGCTGCTACAAGACC TTGGTGAGGCCAAGAATAAATACATTATCTTTCAGTCATCTGTAAATGCCCTCACTCAAGAAGTAGAAGC TGGCAAACAGAAACTAGAGAAGGAGGAGGAAGAGGTCAGGACACTGAAAGAACAACTTAAAGGTCAAGAG CAGCTTGTGTGTAAACTCGCCCGAGTGGAAGGAGAGCAGCAACTCTGTCAGAAGCAGAAACTAGAGCTGA GAAGTCTGACTATGGAGCTGGAGCAGAAGGTCAAAGTGCTGCAGTCTGAAAACGACGCGCTGCAGACCAC CTACGAAGCACTGCAGAGTTCCTACAAGAGTTTAGAAGGTGAACTTGGGTTGATAAAGATGGAGAAAATG GCGCTGGTGGAAAGAGTTAACACAATGACTGGGAAGGAAGCAGAGCTACAGAGGGAGCTGCATGACGTGG AACAGAAAAGTACGCAGCTGAAAGAAGAGTACAGCAAGGAGAAAACCAGGCTAACGGAAGACTTAGAGGT GGTGATGGAAGAGCTGAAGAACACCAAAGTAGCACACCTTAAAAATGTGAATCATCTTGAGAAGGAATTT CAGCGTGCTCAGGGGAAAATAAAATTGCTGCTCAAATCCTGTAAACAGCTGGAAGGAGAAAAGAAAATGT TGCAGAAAGAGCTCTCGCAGCTTGAAGCTGCACAGAAGCAGAGAGCAGGTTCTCTTGTGGATAGTAATGT GGATGAATTAATGACTGAGAACAAAGAGCTGAAAGAGACTCTTGAAGAAAAAATCAAGGAAGCAGATAAG TACTTGGATAAGTACTGTTCCCTTCTTATAAGCCATGAGGAGCTAGAGAAAACCAAAGAGATATTAGAAA TACAAGTCGCTCGACTCAGTTCACGGCAGAACAAACAGGATCTCCAGAGTTCTCCTTTGCTGGATTCTTC GGTTGCAGGACCATCTCCAAGTACTTCTGTCAGCGAGAGGAAGTCAACATCTGGCCTAAATAAAACTTTA GGCAAGAGGCAAAGGTCCAGTGGAATTGGGGAGAACGGGAATGGGACAGCACCTTCTACACCAGAGACGT TCTCTAAGAAAAGCAGGAAATCTGTCAGTAATAGTGCTCACCCTGCTGAGGACGAGGAAGAGACTGAGTT TGAGCCAGAAGGACTCCCAGAAGTTGTTAAAAAAGGGTTTGCTGACATCCCGACTGGAAAGACAAGCCCA TATATCCTTCGGAGAACAACTATGGCAACCAGAACAAGCCCCCGCCTTGCTGCACAGAAGTTATTGGGAT CTTCCCCAACTCTGGGCAAAGAAAATCTTGTAGAGTCCTCCAAACCAACAGCTGGTGGCAGCAGATCACA AAAGGTCAAGGTTGTTCAGGAGAGCTCAATGGATGCACATGCTGTCTTCCAGGAACTCCCAGCAAAATCT CTCACAGCCAATAATATGCCTGGGAGAAACTCTACAGAGAGCCCCAGGGAGGGCCTGAGGGCCAAGCGGG CCTGCCCTGCCTCCAGCCCAGCCGCTGGGCCTGACCCCATGAACAATGAAAACTGCCGAGTCCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001100827
Insert Size	8958 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001100827.1, NP_001094297.1
RefSeq Size	10069 bp
RefSeq ORF	8958 bp
Locus ID	257649

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