Flnc (NM_001191862) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	ABP-L; FLN-C
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217789 representing NM_001191862 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGAACAACAGCAACTACTCCGACGCCTCCGGCCTCGGCCTGCTTGACGAGGCGGACGAGATGCCGT CCACCGAGAAGGACCTAGCGGAGGACGCGCCGTGGAAGAAGATCCAGCAAAACACATTCACGCGGTGGTG CAACGAGCATCTCAAGTGTGTGGGCAAGCGGCTGACCGACCTGCAGCGAGACCTCAGCGACGGGCTGCGC CTCATCGCGCTGCTCGAAGTGCTCAGCCAGAAGCGCATGTACCGCAAGTTCCACCCGCGCCCCAACTTCC GCCAGATGAAGCTGGAAAATGTGTCTGTGGCCCTGGAGTTCCTGGAGCGCGAACACATCAAGCTCGTGTC CATCGACAGCAAGGCCATCGTGGATGGGAACCTGAAGCTAATCCTGGGCCTGATCTGGACACTGATCCTT CACTACTCCATCTCTATGCCCATGTGGGAGGATGAAGACGATGAGGATGCCCGCAAACAGACGCCCAAGC AGCGACTGCTCGGCTGGATCCAGAACAAAGTGCCCCAGCTGCCCATTACCAACTTTAACAGGGACTGGCA GGACGGCAAGGCTCTGGGTGCCCTGGTGGACAACTGTGCCCCTGGTCTCTGCCCTGACTGGGAAGCCTGG GACCCCAACCAGCCTGTGCAGAATGCCAGAGAAGCCATGCAGCAAGCCGATGACTGGCTCGGGGTGCCCC AGGTGATTGCCCCTGAAGAGATCGTGGATCCCAACGTGGATGAGCACTCTGTCATGACCTACCTGTCCCA GTTCCCCAAGGCCAAGCTCAAACCCGGTGCCCCTGTCCGCTCTAAGCAGCTGAATCCCAAGAAAGCCATT GCCTACGGGCCCGGCATTGAGCCCCAGGGCAACACCGTGCTGCAGCCTGCCCACTTCACTGTGCAGACAG TAGATGCTGGTGTGGGTGAGGTGCTGGTCTACATCGAGGACCCCGAGGGTCACACTGAGGAGGCCAAAGT GGTTCCCAACAACGACAAGGACCGCACATACGCAGTCTCCTATGTGCCCAAGGTTGCTGGGTTACACAAG GTGACGGTCCTCTTCGCTGGCCAGAACATCGAGCGTAGTCCCTTTGAGGTGAATGTGGGCATGGCTCTCG GGGATGCCAACAAGGTATCAGCCCGTGGCCCTGGTCTGGAACCTGTGGGTAATGTGGCCAACAAACCCAC CTACTTTGACATCTATACTGCTGGGGCTGGCACTGGTGATGTTGCCGTGGTGATCGTGGACCCACAGGGG CGGCGAGATACAGTAGAGGTGGCCCTGGAGGACAAAGGTGACAACACATTCCGATGCACATACAGGCCCG TGATGGAAGGGCCACACACCGTGCATGTGGCCTTTGCCGGTGCACCCATCACCCGGAGTCCCTTCCCCGT CCATGTGGCAGAAGCTTGTAATCCCAACGCCTGCAGAGCCTCTGGGCGAGGCCTGCAGCCCAAAGGTGTG CGTGTGAAGGAGGTGGCTGACTTCAAGGTGTTCACCAAGGGTGCTGGCAGCGGGGAACTCAAGGTCACAG TCAAGGGTCCAAAGGGCACAGAGGAGCCAGTGAAGGTTCGGGAGGCTGGAGACGGTGTGTTCGAGTGCGA GTACTACCCAGTGATACCTGGGAAGTATGTGGTGACCATCACTTGGGGTGGCTATGCCATCCCCCGCAGT CCTTTTGAGGTCCAGGTGAGCCCAGAGGCCGGAGCCCAGAAAGTACGGGCATGGGGTCCTGGTTTGGAGA CTGGCCAGGTGGGCAAGTCAGCTGACTTCGTGGTGGAAGCCATTGGCACAGAAGTGGGGACGCTGGGCTT CTCTATTGAGGGGCCCTCGCAAGCCAAGATCGAGTGTGATGACAGGGGTGATGGCTCCTGTGACGTGCGG TACTGGCCCACGGAGCCCGGGGAGTATGCTGTGCACGTCATCTGTGACGATGAAGACATCCGGGACTCAC CTTTCATCGCCCACATTCAGCCAGCCCCACCAGACTGCTTCCCAGACAAGGTGAAAGCCTTCGGACCTGG TCTGGAGCCCACTGGCTGCATCGTAGACAGGCCTGCGGAGTTCACCATTGATGCCCGTGCTGCTGGCAAG GGAGATTTGAAGCTCTATGCCCAGGATGCAGATGGCTGCCCCATCGACATCAAGGTGATCCCCAATGGTG ATGGCACCTTCCGCTGCTCCTACGTGCCTACCAAGCCCATTAAGCATACCGTCATTATCTCTTGGGGAGG TGTCAACGTGCCCAAGAGCCCCTTCAGGGTGAATGTAGGAGAAGGCAGCCATCCTGAGCGGGTAAAAGTA TATGGCCCTGGAGTGGAGAAGACAGGCCTGAAAGCCAACGAACCCACCTATTTCACAGTGGACTGCAGTG AAGCTGGGCAAGGTGATGTGAGCATTGGCATCAAATGTGCCCCTGGCGTGGTGGGGCCCGCGGAAGCTGA CATCGATTTTGACATCATCAAGAATGACAATGACACCTTCACAGTCAAGTACACACCCCCAGGGGCCGGC CACTACACGATCATGGTGCTGTTTGCCAACCAGGAAATCCCTGCCAGTCCCTTCCACATCAAGGTGGACC CGTCCCACGATGCTAGCAAGGTCAAGGCTGAGGGCCCTGGGCTGAGCCGCACAGGTGTGGAAGTCGGAAA GCCAACTCACTTCACTGTGCTGACCAAGGGAGCTGGCAAGGCCAAACTGGACGTGCACTTCGCTGGGGCA GCCAAGGGCGAGGCTGTGCGAGACTTTGAAATCATCGACAACCATGACTATTCGTATACTGTCAAGTACA CTGCTGTCCAGCAGGGCAACATGGCAGTAACGGTGACCTACGGTGGAGACCCTGTCCCCAAAAGTCCCTT CGTGGTGAATGTGGCACCCCCACTGGACCTCAGCAAAGTCAAAGTCCAAGGCCTGAACAGCAAGGTAGCT GTGGGAGAGGAACAAGCGTTCTTGGTGAACACCAGAGGGGCTGGTGGTCAGGGCCAACTAGATGTGCGGA TGACCTCACCCTCCCGACGGCCTATCCCCTGCAAGCTGGAACCTGGGAGTGGAGCAGAAGCCCAGGCTGT CCGCTACATGCCCCCTGAAGAAGGACCTTACAAGGTGGACATCACCTATGATGGTCACCCAGTGCCTGGC AGCCCCTTTGCTGTGGAAGGCGTCCTGCCCCCTGACCCCTCCAAGGTCTGCGCGTATGGCCCTGGTCTCA AGGGTGGGCTAGTAGGCACCCCGGCACCATTCTCCATTGACACCAAGGGGGCCGGCACAGGAGGCCTGGG GCTGACTGTCGAGGGCCCCTGCGAAGCCAAGATCGAGTGCCAAGACAATGGTGATGGATCTTGTGCAGTT AGCTACCTGCCCACAGAGCCAGGCGAGTACACCATCAATATCCTGTTCGCTGAAGCCCACATCCCTGGCT CACCCTTCAAGGCCACCATCCAGCCTGTGTTCGACCCAAGCAAGGTGAGGGCCAGTGGGCCAGGCCTGGA GCGTGGCAAGGCCGGAGAGGCAGCCACCTTCACTGTGGACTGCTCGGAAGCGGGCGAGGCCGAGCTGACC ATCGAGATCCTGTCAGACGCTGGTGTCAAAGCGGAGGTGCTGATCCACAACAACGCGGATGGCACCTACC ACATCACCTACAGTCCTGCCTTCCCAGGCACCTACACCATCACCATCAAGTATGGCGGCCACCCCATACC CAAGTTCCCCACCCGTGTCCACGTGCAGCCTGCAGTTGATACCAGTGGCATCAAGGTCTCTGGGCCTGGT GTGGAACCACATGGTGTCCTGCGGGAGGTGACCACCGAATTTACTGTGGATGCAAGATCTCTGACAGCTA CAGGTGGCAACCACGTGACAGCTCGTGTGCTCAACCCCTCGGGTGCTAAGACAGATACCTATGTGACAGA CAACGGGGATGGCACCTACCGGGTGCAGTACACGGCCTACGAAGAGGGTGTGCATTTGGTGGAGGTGCTG TATGACGAAGTAGCCGTGCCCAAGAGCCCCTTCCGAGTGGGTGTGACGGAGGGCTGTGACCCCACCCGAG TCCGGGCCTTCGGACCAGGCCTAGAGGGTGGCTTGGTCAACAAGGCCAACCGCTTCACTGTGGAAACTAG GGGTGCTGGCACAGGAGGCCTTGGCCTAGCCATCGAGGGCCCCTCAGAAGCTAAGATGTCCTGCAAGGAC AACAAAGATGGCAGTTGCACTGTGGAGTACGTCCCCTTCACTCCTGGGGACTATGATGTCAACATCACCT TTGGGGGACAACCCATCCCAGGAAGCCCATTCCGAGTTCCTGTGAAGGATGTTGTGGACCCTGGGAAGGT GAAGTGTTCAGGGCCAGGGCTAGGGACTGGCGTCAGGGCTCGGGTACCTCAGACCTTCACAGTGGACTGC AGTCAAGCTGGCCGGGCCCCTCTACAGGTGGCAGTGCTGGGACCCACAGGTGTGGCTGAGCCTGTGGAGG TGCGTGACAATGGCGACGGTACCCACACTGTCCACTACACGCCTGCCACTGACGGACCCTATACAGTAGC TGTCAAGTATGCTGACCAGGAGGTGCCACGCAGCCCTTTCAAGATCAAAGTGCTTCCGGCCCACGATGCC AGCAAAGTGCGGGCCAGTGGCCCTGGCCTCAATGCCTCCGGCATCCCCGCCAGCCTGCCCGTGGAGTTCA CCATTGATGCTCGTGATGCTGGTGAGGGGCTTCTCACAGTCCAGATCCTGGATCCCGAGGGTAAACCCAA GAAGGCCAACATCCGAGACAATGGGGATGGCACATACACCGTGTCCTACCTGCCAGACATGAGTGGTCGG TACACCATCACCATCAAGTATGGCGGCGATGAGATCCCTTACTCACCCTTCCGTATCCACGCCCTGCCTA CAGGGGATGCCAGCAAGTGCCTTGTTACAGTATCCATTGGAGGCCATGGACTGGGTGCCTGCCTGGGTCC CAGAATCCAGATTGGAGAGGAGACTGTGATTACAGTGGATGCCAAGGCAGCAGGCAAGGGGAAGGTGACC TGCACAGTATCCACACCTGACGGGGCAGAGCTCGATGTGGATGTAGTTGAGAACCACGATGGTACCTTTG ATATCTACTACACAGCACCTGAGCCGGGCAAATATGTCATCACCATCCGCTTTGGGGGTGAACACATTCC CAACAGCCCCTTCCATGTGCTGGCATGTGACCCGCTGCCTCATGTGGAGGAGCCTGCGGAAGTGCTGCAG CTGCACCAGCCATATGCCCCACTTCGGCCTGGCACCTGCCCCACACACTGGGCCACAGAGGAGCCCGTGG TTCCTGTGGAGCCTTTGGAGTCCATGCTGAGGCCTTTCAACTTGGTCATCCCATTCACTGTGCAGAAAGG GGAGCTGACAGGGGAGGTCCGGATGCCCTCTGGTAAAACAGCACGTCCCAACATCACTGACAACAAGGAC GGTACCATCACAGTGAGATACGCACCTACAGAGAAAGGCCTGCACCAGATGGGAATCAAGTACGATGGCA ACCATATCCCTGGGAGCCCTCTGCAGTTCTATGTGGATGCCATCAACAGCGGGCATGTCAGTGCCTATGG GCCAGGTCTGAGCCATGGCATGGTCAACAAGCCTGCCACCTTCACAATTGTCACCAAAGACGCTGGGGAA GGGGGGCTGTCACTGGCTGTGGAGGGCCCATCCAAGGCGGAGATCACCTGCAAGGACAACAAAGATGGCA CCTGTACCGTGTCCTACCTACCCACAGCACCTGGAGACTACAGCATCATTGTGCGCTTTGACGACAAGCA TATCCCAGGGAGCCCCTTCACGGCCAAGATCACAGGTGACGACTCAATGAGAACGTCCCAGCTTAATGTG GGCACCTCCACCGATGTGTCACTGAAGATCACAGAGGGTGACCTCAGCCAGCTGACAGCCAGTATCCGTG CTCCTTCAGGCAATGAGGAGCCTTGCCTGCTGAAGCGCTTGCCCAACAGGCACATTGGGATCTCCTTTAC CCCCAAGGAGGTCGGTGAGCATGTGGTGAGCGTACGCAAGAGTGGCAAGCACGTCACCAACAGCCCCTTT AAGATTTTGGTTGGGCCTTCGGAGATCGGCGATGCCAGCAAAGTTCGAGTCTGGGGCAAGGGCCTTTCCG AGGGACAGACCTTCCAAGTGGCAGAGTTCATTGTGGACACTCGCAATGCAGGCTACGGAGGCCTGGGGCT GAGCATCGAAGGTCCTAGCAAGGTGGACATTAACTGTGAGGATATGGAAGACGGTACCTGCAAAGTCACC TACTGTCCAACTGAACCTGGCACCTACATCATCAACATTAAGTTTGCTGACAAACACGTGCCTGGAAGTC CCTTCACCGTGAAGGTGACTGGCGAGGGACGCATGAAGGAGAGCATCACCCGGCGCAGACAGGCGCCTTC CATCGCCACCATTGGTAGCACCTGTGACCTCAACCTCAAGATCCCAGGAAACTGGTTTCAGATGGTGTCC GCCCAGGAGCGCCTGACTCGCACCTTCACGCGTAGCAGTCACACGTACACCCGCACGGAGCGGACAGAGA TCAGCAAGACTCGGGGTGGGGAGACCAAGCGTGAAGTCCGGGTGGAGGAGTCCACCCAGGTCGGCGGAGA CCCTTTCCCTGCTGTTTTCGGCGATTTTCTCGGCCGGGAACGCCTGGGCTCCTTTGGAAGCATCACCCGA CAGCAGGAAGGTGAGGCCAGCTCTCAGGACATGACAGCCCAGGTGACCAGTCCATCTGGCAAGACAGAAG CCGCAGAGATCGTTGAGGGAGAAGACAGCGCATACAGTGTGCGCTTTGTGCCCCAGGAGATGGGCCCCCA CACAGTCGCTGTCAAGTACCGGGGCCAACATGTGCCTGGAAGCCCTTTTCAGTTCACCGTGGGTCCGCTA GGTGAAGGTGGTGCCCACAAGGTGCGGGCTGGAGGCACAGGGCTGGAGCGAGGTGTAGCTGGTGTGCCAG CTGAGTTTAGCATCTGGACCCGAGAAGCTGGAGCTGGGGGCCTGTCCATTGCTGTGGAAGGTCCCAGCAA GGCAGAGATCGCATTCGAAGACCGCAAAGATGGTTCTTGTGGTGTCTCCTATGTTGTCCAGGAGCCAGGT GACTATGAGGTCTCCATCAAGTTCAATGATGAGCACATCCCAGACAGCCCCTTTGTGGTGCCTGTGGCCT CCCTCTCAGATGATGCTCGCCGCCTCACCGTCACCAGCCTCCAGGAGACAGGGCTTAAGGTGAACCAGCC AGCATCTTTCGCGGTGCAGCTTAATGGGGCACGTGGCGTGATCGATGCCCGGGTACACACACCCTCAGGA GCTGTGGAGGAGTGCTATGTCTCTGAACTGGACAGTGATAAGCATACCATCCGCTTCATCCCCCATGAGA ACGGTGTCCACTCAATTGATGTCAAGTTCAACGGTGCCCACATCCCTGGAAGCCCCTTCAAGATCCGCGT TGGGGAGCAGAGCCAAGCTGGGGACCCAGGCTTGGTGTCAGCTTATGGGCCTGGGCTTGAAGGAGGCACT ACGGGTGTATCGTCAGAGTTCATTGTTAACACCCAGAATGCAGGCTCGGGGGCCTTGTCTGTCACCATCG ATGGGCCTTCCAAGGTGCAGCTGGACTGTCGGGAGTGTCCTGAGGGCCATGTAGTCACTTACACGCCCAT GGCTCCTGGCAACTACCTCATTGCCATCAAGTATGGCGGCCCTCAGCACATTGTGGGCAGTCCCTTCAAA GCAAAGGTCACAGGTCCCCGGTTGTCTGGAGGCCACAGCCTTCACGAAACATCCACAGTTCTGGTGGAGA CTGTGACAAAGTCCTCCTCGAGCCGCGGCGCCAGCTACAGTTCCATCCCCAAGTTCTCCTCAGATGCCAG CAAGGTGGTGACAAGGGGCCCCGGCCTGTCCCAGGCCTTTGTGGGTCAGAAGAACTCCTTCACCGTGGAC TGCAGCAAAGCAGGTACCAACATGATGATGGTGGGTGTGCACGGGCCCAAGACCCCCTGCGAAGAGGTAT ATGTGAAGCACATGGGGAACCGGGTCTACAATGTCACCTACACTGTCAAGGAGAAAGGAGACTACATCCT CATTGTCAAGTGGGGTGATGAAAGTGTCCCTGGAAGCCCCTTCAAGGTCAATGTGCCCTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001191862
Insert Size	8181 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191862.1, NP_001178791.1
RefSeq Size	9049 bp
RefSeq ORF	8181 bp
Locus ID	362332
UniProt ID	D3ZHA0
Gene Summary	Muscle-specific filamin, which plays a central role in muscle cells, probably by functioning as a large actin-cross-linking protein. May be involved in reorganizing the actin cytoskeleton in response to signaling events, and may also display structural functions at the Z lines in muscle cells. Critical for normal myogenesis and for maintaining the structural integrity of the muscle fibers.[UniProtKB/Swiss-Prot Function]

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