Prpf8 (NM_001191590) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217763 representing NM_001191590 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGAGTGTTTCCTTACAGAGGGCCGGGTAACCCGGTGCCCGGCCCTCTAGCCCCGCTGCCGGATT ACATGTCAGAGGAGAAGCTGCAGGAGAAAGCTCGAAAATGGCAGCAGTTGCAGGCCAAGCGCTATGCAGA GAAGCGGAAATTCGGGTTTGTGGATGCTCAGAAGGAAGACATGCCCCCAGAACATGTCAGGAAAATCATT CGAGACCATGGAGACATGACCAACAGGAAGTTCCGTCATGACAAAAGGGTCTATTTGGGAGCTCTCAAGT ACATGCCTCACGCAGTTCTCAAGCTCCTAGAGAACATGCCCATGCCTTGGGAGCAGATCCGGGATGTGCC TGTGCTGTACCACATCACTGGAGCCATTTCCTTCGTTAACGAGATTCCCTGGGTTATTGAGCCAGTTTAC ATCTCCCAGTGGGGGTCAATGTGGATCATGATGCGTAGAGAAAAAAGAGACAGGAGGCATTTCAAGAGGA TGCGTTTTCCCCCTTTTGATGATGAGGAGCCGCCCCTGGATTATGCTGACAACATCTTAGATGTCGAGCC ACTGGAAGCCATTCAGCTAGAGCTGGACCCTGAGGAGGATGCCCCTGTGTTGGACTGGTTCTATGACCAC CAGCCATTGAGAGATAGTAGGAAGTATGTTAATGGTTCCACCTACCAACGCTGGCAGTTCACATTGCCTA TGATGTCCACTCTCTACCGACTGGCCAACCAGCTTCTGACTGACTTGGTAGATGACAACTACTTTTATCT CTTTGATCTAAAGGCCTTCTTTACCTCTAAGGCACTCAACATGGCCATTCCTGGGGGACCCAAATTTGAA CCTCTTGTTCGAGACATCAACCTACAGGATGAAGACTGGAATGAATTCAATGATATTAACAAGATCATCA TCCGTCAGCCTATTCGTACTGAGTACAAGATTGCTTTTCCGTACCTCTATAACAACCTCCCACACCATGT CCACCTGACCTGGTACCACACTCCTAACGTTGTGTTCATCAAGACTGAGGATCCTGATCTGCCAGCTTTC TACTTTGATCCTCTGATTAACCCAATCTCCCATAGACACTCAGTCAAGAGCCAAGAGCCACTACCTGATG ATGATGAGGAGTTTGAGCTCCCAGAGTTTGTGGAGCCCTTCCTGAAGGATACACCTCTCTATACAGACAA TACAGCCAATGGCATCGCACTGCTCTGGGCACCACGGCCCTTCAACCTACGCTCTGGTCGTACCCGCCGG GCCCTCGACATTCCCCTTGTCAAGAACTGGTATCGGGAGCACTGTCCTGCTGGGCAACCTGTGAAAGTTC GAGTCTCCTATCAGAAGCTGCTTAAGTATTATGTGCTGAATGCCCTCAAACATCGGCCTCCCAAAGCGCA GAAGAAGAGGTATCTGTTTCGCTCTTTCAAAGCTACCAAATTCTTTCAGTCTACCAAGCTGGACTGGGTG GAGGTCGGGCTGCAGGTTTGCCGCCAGGGCTACAACATGCTTAACCTTCTCATTCACCGTAAGAACCTCA ACTATCTGCATTTGGACTATAACTTCAACCTGAAGCCTGTCAAGACGCTCACCACCAAGGAAAGAAAAAA ATCTCGCTTTGGGAATGCTTTCCATCTGTGTCGGGAAGTACTGCGTTTGACTAAGCTGGTTGTGGATAGT CATGTGCAGTATCGCTTGGGCAATGTAGATGCCTTCCAGCTGGCAGATGGATTGCAGTATATATTTGCCC ACGTGGGACAGTTGACAGGCATGTATCGATACAAATATAAGCTGATGCGGCAGATCCGCATGTGCAAGGA CTTGAAACATCTCATCTATTACCGCTTCAACACGGGTCCTGTGGGGAAAGGCCCTGGCTGTGGTTTCTGG GCTGCTGGCTGGCGGGTCTGGCTATTTTTTATGCGTGGAATTACCCCTTTGCTAGAACGATGGCTAGGCA ACCTTCTGGCCCGACAGTTTGAAGGCCGTCACTCAAAGGGGGTGGCAAAGACAGTAACAAAGCAACGAGT AGAGTCCCATTTTGACCTTGAGCTTAGGGCAGCTGTGATGCATGATATTCTGGACATGATGCCTGAAGGC ATCAAACAGAACAAGGCCCGAACAATTCTGCAGCACCTCAGTGAGGCCTGGCGCTGCTGGAAGGCCAACA TTCCATGGAAGGTCCCAGGACTGCCAACGCCTATAGAGAATATGATTCTTCGGTACGTTAAGGCCAAGGC TGATTGGTGGACCAATACTGCCCACTACAACCGTGAACGGATTCGCCGTGGGGCTACTGTAGACAAGACT GTTTGCAAAAAGAACCTGGGCCGCCTCACCCGCCTGTATCTGAAGGCAGAACAGGAGCGGCAGCACAATT ACCTGAAGGACGGGCCTTACATCACAGCAGAGGAAGCAGTGGCAGTGTATACTACCACTGTGCACTGGCT GGAAAGCCGTAGGTTCTCTCCTATCCCGTTCCCCCCACTCTCCTACAAGCATGACACCAAGTTGCTTATC TTGGCTCTGGAGCGACTCAAGGAAGCTTATAGTGTGAAGTCTAGGTTAAACCAGTCTCAGAGGGAGGAGC TGGGTCTGATTGAACAAGCCTATGACAACCCCCATGAGGCTTTGTCCCGCATCAAGCGTCACCTCCTCAC GCAGAGAGCCTTTAAGGAAGTGGGCATTGAGTTTATGGATCTCTATAGCCACCTTGTGCCAGTGTATGAT GTGGAACCACTGGAGAAAATAACTGATGCTTACCTGGACCAGTACCTGTGGTATGAAGCAGATAAACGCC GTCTTTTCCCGCCCTGGATCAAGCCTGCTGACACAGAACCCCCTCCACTGCTTGTTTACAAGTGGTGCCA AGGCATCAATAACCTGCAGGATGTGTGGGAGACGAGTGAGGGTGAGTGCAATGTCATGCTGGAGTCTCGG TTTGAGAAGATGTATGAGAAGATTGACCTGACCCTGCTCAACAGGCTGCTGCGACTCATTGTGGACCACA ACATAGCTGACTACATGACAGCCAAGAACAATGTAGTCATCAACTATAAGGACATGAACCATACAAATTC ATATGGAATCATCAGAGGCCTGCAGTTTGCCTCATTCATTGTACAGTACTATGGTTTGGTGATGGATTTG CTTGTATTGGGATTGCACCGGGCCAGTGAGATGGCTGGGCCTCCGCAGATGCCAAATGACTTTCTCAGTT TTCAGGACATAGCCACCGAGGCTGCACACCCAATCCGACTCTTCTGTAGATACATTGATCGCATCCATAT TTTCTTCAGGTTCACAGCAGATGAGGCTCGGGATCTGATCCAGCGTTACCTGACAGAACATCCAGACCCC AATAATGAAAACATTGTTGGCTACAATAATAAGAAATGCTGGCCCCGAGATGCCCGTATGCGTCTCATGA AGCATGATGTCAACTTGGGTCGGGCAGTGTTCTGGGACATCAAAAACCGGCTGCCACGATCGGTGACTAC AGTACAGTGGGAGAATAGCTTTGTGTCTGTGTACAGTAAAGACAACCCCAACCTGCTGTTCAACATGTGT GGCTTTGAGTGCCGCATCCTACCTAAGTGCCGTACCAGCTATGAAGAGTTCACCCACAAGGACGGGGTCT GGAATCTACAGAATGAGGTTACTAAGGAGCGGACGGCTCAGTGTTTCCTGCGTGTGGACGATGAGTCAAT GCAGCGCTTCCATAACCGCGTGCGGCAGATTCTGATGGCCTCTGGCTCTACCACTTTCACCAAGATTGTG AATAAGTGGAATACAGCTCTCATTGGCCTTATGACATACTTTCGAGAGGCTGTGGTGAATACACAGGAAC TCCTAGACTTACTGGTGAAGTGTGAAAACAAGATCCAGACACGTATCAAGATTGGATTGAACTCCAAGAT GCCAAGTCGGTTCCCCCCTGTTGTATTCTACACCCCTAAGGAGCTGGGTGGGCTTGGCATGCTCTCGATG GGCCATGTCCTTATCCCCCAGTCTGACCTCAGGTGGTCCAAGCAAACAGATGTGGGCATCACACACTTTC GTTCAGGAATGAGTCATGAAGAAGACCAACTGATTCCCAACTTGTACCGCTACATACAGCCATGGGAGAG TGAATTCATTGACTCCCAGCGGGTCTGGGCTGAGTATGCACTCAAGCGACAGGAGGCTATTGCTCAGAAC AGACGCTTGACTTTAGAAGATTTAGAAGACTCGTGGGATCGGGGCATTCCCCGGATCAATACCCTCTTCC AGAAAGATCGGCACACATTGGCTTATGATAAAGGCTGGCGTGTCCGAACTGACTTTAAGCAGTACCAGGT TTTGAAGCAGAACCCCTTTTGGTGGACACATCAACGGCATGATGGCAAGCTTTGGAACCTCAACAACTAC CGCACAGACATGATCCAGGCACTGGGCGGTGTGGAGGGCATTCTGGAACACACTCTCTTCAAGGGCACTT ACTTCCCAACCTGGGAAGGTCTGTTCTGGGAGAAAGCCAGCGGGTTTGAGGAATCCATGAAGTGGAAGAA GCTGACCAATGCTCAGCGATCAGGATTAAACCAGATTCCTAATCGTAGATTTACTCTCTGGTGGTCCCCA ACAATCAATCGAGCCAATGTATACGTAGGCTTCCAGGTGCAGCTGGACCTGACAGGAATCTTTATGCATG GCAAGATCCCCACGCTGAAGATCTCTCTCATCCAGATCTTCCGAGCTCACTTGTGGCAAAAGATTCATGA GAGCATCGTGATGGACTTGTGTCAGGTCTTCGACCAGGAATTAGATGCCCTGGAAATTGAGACAGTACAA AAGGAGACAATCCATCCCCGAAAATCCTACAAGATGAACTCTTCCTGTGCAGATATACTGCTCTTTGCTT CCTATAAGTGGAATGTCTCCCGGCCCTCGCTGCTGGCAGACTCTAAGGATGTGATGGACAGCACCACCAC ACAGAAGTATTGGATTGACATCCAGTTACGCTGGGGGGACTATGATTCCCATGATATTGAGCGCTATGCC CGGGCCAAGTTCCTGGATTACACCACAGACAACATGAGTATCTACCCTTCACCCACAGGTGTGCTCATTG CCATTGATCTGGCCTATAATCTACACAGTGCCTATGGAAACTGGTTCCCGGGGAGCAAGCCTCTCATACA GCAAGCCATGGCCAAGATCATGAAGGCAAACCCTGCCTTGTATGTGTTACGTGAACGGATACGCAAAGGA TTGCAGCTGTATTCATCTGAGCCCACTGAGCCTTACCTGTCCTCTCAGAACTATGGTGAACTCTTCTCGA ACCAGATTATCTGGTTTGTGGATGACACCAATGTCTATAGAGTAACTATCCACAAGACCTTTGAGGGGAA CTTGACAACCAAACCCATCAATGGGGCCATCTTCATCTTCAATCCACGCACAGGACAGCTGTTCCTCAAG ATAATCCACACATCCGTATGGGCTGGACAGAAGCGTTTGGGTCAGTTGGCCAAGTGGAAGACTGCTGAAG AAGTGGCTGCCCTGATCCGATCTCTGCCTGTGGAGGAACAGCCTAAACAGATTATTGTCACTCGGAAAGG GATGCTGGATCCCCTGGAGGTACACTTACTGGACTTCCCCAATATTGTGATCAAAGGATCAGAGCTCCAG CTGCCCTTCCAGGCTTGTCTCAAGGTGGAGAAGTTTGGGGATCTCATCCTTAAAGCCACAGAACCACAGA TGGTTCTCTTCAACCTCTATGATGACTGGCTCAAGACTATCTCATCCTACACGGCTTTCTCCCGTCTCAT CCTGATCCTGCGTGCTTTGCATGTGAACAACGATAGGGCAAAAGTGATCCTGAAGCCAGACAAGACAACC GTTACGGAACCCCACCACATCTGGCCCACGCTCACTGACGAGGAGTGGATCAAGGTGGAGGTGCAGCTCA AGGATCTGATCTTGGCTGACTATGGCAAGAAAAACAATGTGAATGTGGCATCGCTGACACAATCAGAAAT TCGAGACATCATCCTGGGAATGGAGATCTCTGCACCATCGCAGCAGCGGCAGCAGATAGCTGAGATTGAG AAGCAGACCAAGGAACAGTCCCAGCTCACTGCTACACAGACTCGAACTGTCAACAAGCATGGCGATGAGA TCATCACCTCCACCACTAGCAACTATGAGACCCAGACCTTCTCGTCGAAGACCGAATGGAGAGTCAGGGC CATCTCTGCTGCCAACCTGCATCTACGGACCAATCACATTTATGTTTCATCTGATGACATCAAGGAGACT GGTTATACGTACATTCTTCCCAAAAATGTGCTGAAGAAGTTCATCTGCATATCTGATCTGCGCGCCCAAA TTGCAGGATACCTATATGGGGTGAGCCCTCCAGATAACCCTCAGGTGAAGGAGATCCGCTGTATTGTGAT GGTGCCACAGTGGGGCACTCACCAGACTGTGCACCTGCCTAGTCAGCTGCCTCAACATGAGTACCTCAAG GAGATGGAACCCTTAGGTTGGATCCACACTCAACCCAATGAATCCCCCCAGCTATCGCCCCAAGATGTAA CTACTCATGCCAAAATCATGGCTGACAACCCATCTTGGGATGGGGAGAAGACTATTATCATCACCTGCAG CTTCACACCAGGTTCCTGCACACTGACAGCCTACAAGCTGACCCCTAGCGGGTATGAGTGGGGTCGGCAG AACACAGACAAAGGCAACAACCCCAAAGGCTACCTACCTTCGCACTATGAGAGGGTTCAGATGCTCCTGT CTGACCGATTCCTCGGCTTCTTTATGGTCCCTGCCCAGTCCTCCTGGAACTACAACTTTATGGGTGTTCG ACATGACCCCAACATGAAATACGAGCTGCAGCTAGCAAACCCCAAGGAGTTCTACCATGAGGTCCACAGG CCCTCCCACTTCCTCAACTTTGCCCTCCTGCAGGAGGGTGAGGTCTATTCCGCAGACAGAGAGGACCTCT ATGCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191590
Insert Size	7008 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191590.1, NP_001178519.1
RefSeq Size	7246 bp
RefSeq ORF	7008 bp
Locus ID	287530

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