Spg11 (NM_001191571) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1562529
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217759 representing NM_001191571 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCCAAGCCAGGGCCCGGAAGTGCTGCCTCCCCCGGCTGTGCCGGCGCCATGGAGCGAGTTCTAC CCATGCTGCTGGTGCGGATCCCTGCCGAGGAGACGGCGCAGCTGGGACCGCGGGCGCAGCTGCACCGAGA GCTGGAGGCCCTGGGGAGCCTGACGGCTGCGGGCAGTCTCCAAGTGCTTTCCTTGGCGCCGGGCAGTAGG GGTGGAAGCAGCTGCCGCCTCCAAGGCCCCTTTAGGCAGTTCCTGTGGGAGGAGTCTCAGAACAGCACAT CAGCAGACAAGCCCAGAGTGCTTGTTGCTCGAGAAGATTGTGGGCTGCTCATATATGAATTTGACTTGAG AGATGGAAGATGTGACGCAGCCTTACTACACAGCTGTTGTGGGAGGACCCTGCAGAAGCTCACTGAAGAT CAAGGTGTCAGCATTTCCTTCAAGTCTTTGAGGATTCTGTCATTTGACAACAGCACGTCCTTACTGCTCA TCAATGAGTGTCTCGTCGTACGTGTCGTGTTTCCGGGGAGAGAATCTGGGATTGGAGTGCTGGGCTGCCT CTCACTGCCCTTGCCTGCACAGGCAGTGGACACGATAATTGACGTGCAGCTCTGCAAGAGGTTTCTTTTC CTTCTGAGTACATTAGGCTGGATCCATATTTTTGACACTGTAAAGGGCATGCATGTAGCCCGCGTGGACT TGGCTCTTCCCCGAGAAGATTCTGAGCAGCAGGAGCCTGCCAGCATTTCTTCCTTTATGTCACTGAAAGT GTCTCGGGACTTAGATGTTTTGCTACTCGTCAGCTCGTCCAGTACTGCAGTTGCTCTTAACCTTCACTTG TATTTCAGGCTACACCCAGGGCACCTGCTGTGTGAAGGAACTCTGCAAGACCTCCCTATTGAAGGGCCTA CAGGAATAGATGAAGATGACCTTGTTAATTCTGCCCACAACATGAAACTGACCAAGGTGTCCTTCCAAGT TGATAGGTCCTGGAGAGCCCAGCTGGCATCACTGAATGAATCTGTGAGGAGCTCAGAACCAGAGCTTTCC TGCTATCCTCCGTGGTTCCAGGGTGCTTTGTGTTTGGAGTCCCTGGAATCTGGTAACCACTCCCCCATTG TGCCAAACCACGTTTTCATTCCAGGGGATGTCCCGAGTGGCCAGTATGCTTTTCCTCAGAAGGAGCACAG CAAGACCAGTGAGCCAAGGCCGTGGAGGACAATGCACCTGAGTGAACAAGAGCAGCCCACAGAGCTCACG TGTCTGTCTGTGACGGGATTTACGGCCCTGTTTATTCAGGCAGTAGGAACGACCAGCTGCACCGTCGTCC TCTGGGACTTGGAGACCCAGAGCATGCAGTGTTTTTCTCTCAGCCAGAAGTGTACTCCTGTAGACATCAG CGGAGACCAGCAGCTGTGCCTTGCTTTGACAGAGAACGGCCTGTCTCTGATTTTGTTTGGTTTAACTCAA GAGGAGTTTCTGAACAGACTGATGATCCATGGCAGTGCCAGCACTGTGGACTCCCTTTGTCATCTCAACG GTTGGGGACGGTGCTCAATCCCCATCCACGCTCTAGAGGCTGGCATAGAAAATCGTCAGCTGGACACGGT AGATTTCTTCCTGAAGAGCAAGGAAAATCTTCTTACTCCATCTTCAAAATCTCCTGCCCCTGATCAGCAG CAGCACTTCTCCTCCCAGCTCTACCTAAGGCAGGTGGAAGAGATGACCCCAGCCCTGGACTTACTCTGCT CAGCAATTAGAGAAAGCGACTCCGAAACCCAAAGCAAGCACTTTGCAGAGCAGCTGCTTCACCTCACACT GTCTTTCCTTAACAAGCAAATGAGGGAGCTTTTTGATCACACTGAAGAGCTAGATGAGCATTTACAGAAA GGAGCAGCCATTTTGACCAGCTACATCAATGAACTTCGGACTTTTATGATAAAGTTTCCTTGGAAGCCAG GAGATGCCATAGATGAATCTGATGAAAATGAAGAGGTCCTCACAGTGAAAGAAGGCCAGGTTTGGGAGGA ACTCCGCTCTGAGGAAGTTATTGCTGATGCCATTTTAAATAACAGAATACCAGAGGCACAAACTTTCTTC AGGATTACTGGTCATTCCGCTCAACGACTTGAGGAACTGGTTAGGATAGGCCTAGATTTGGCATTTGACA GTTTAAAAAAGAACAGCGTGGAGGAGGCCTCCAGGCTCTTGAGGAACATGGGCTTCAGTGTGGAAGACGA GCTGCTTAAGATAAGCTTTTATACGACTGATAAAAACATACGGGACTTTTTGGTTGAAATTTTAAAAGAA AAGGAATGTTTTTCTGAAAAAGAAAGAAGAACTGTGGACTTTGTCCATCAAGCTGAAAGGCTTTACTCAG GACACTTCCAGGAAAATGCGCCGATGCAGGCTTTTCCCAGGTATTGGATAAAGGAACAGGACTGTTTCAA ACACAAATCTGTCTTGGACACGTTCCTCAAATGTGATACAAAAGATGAGTTTACCAAACAGGACCATAGA ATTGCACTGAATTGGGCTCACTGGTGGGATCGGCAAACACAGGAGTGTATCCTTCTCCCCAGAATAAGTT CAGAAGAATATAAAGCACATTCCCCAGAAGCCCTGTGGAGACACCTCACAGCTCGCCATGACTGGTCAAG CATCAGCTTGTGGATGGAAGAATTTCAGGCCCAGGAAACTGTGTCACCTCAGCAGAGCAAGTGGCCTCCT CTGAGTGCTGCCGTCATCGAGCAGAACACTTGCTGCAGCAGGCACATGAGGAACGCGATCTTAGACCAGC TGGCCAGATGTGGCATTTTTCTTGCATCTGAGCTGGAAGACTTTGAACTTCTCCTCCTGAGACTGAGCCG GATTGGGGGTGTAATGCAGGATGCCCTCCCCGTGCAGAGCTACAGATCCTCGGCAGGGTGCAACTTTGAT TCCCAAGTCATCCTCTACTGTTTAGAGCACGGCCTGCAGCATCTTCTCTACGTCTACCTTGACTATTACA AACTTAGCCCTGGAAATTGTCCCTTTTTGGAAAAGAAAGAACTACATGAAGCTCACCCTTGGCTTGAATT TTTGGTTCAGTGTCGGCAAGTTTCCAGTAACTTGACAGATCCCAAACTGATCTTTCAGGCCAGTCTTGCA AATGCTCAGATTTCGATTCCCACCAATCAAGCCAGTGTGAGCAGCATGCTGTTGGAAGGACACACCCTCT TGGCTCTTGCTACCACCATGTATGCTCCTGGGGGCGTCAGCCAGGTCATTCAGCATGAAGACAGCGAGAA CTGTTTGAAGAAAGTGGACCCCCAGCTACTGAAGATGGCCTTAACGCCTTACCCAAAGCTCAAAGCCGCG CTCTTCCCTCAGTGCACTGCTCCTAGTATTCTGCCCTCTGACATTACACTCTACCACCTCATCCAGTCCT TACCACCCTTTGATCCCAGCAGACTGTTTGGCTGGCAGTCTGCCAACACTCTAGCTATCGGAGATGCGGC CGGTCAGCTCCCACACTTCTCCAGTCCTGACCTGGTGAGTAAATACGCCGTGGTGGAGCAGCTGAGCTAC ACGTATTACTTACATCACGGACGCCCATCCTTCGCCTTCGGCACCTTTCTGGTCCAGGAGTTAATCAAGA GTAAGACTCCCAAGCAGCTGATCCAGCAAGTAGGCAAGGAGGCCTATACCTTAGGGCTCTCCTCCTTCCA CAACCCTTCGGTAGGGGCCGCCTGTGTCTGCTTCTTAGAATTGCTTGGCCTCAGCAGCCTCAAGCTCAGA GTTGACCTGAAAATGGCAAACGTAGTTCTGGGCTCCAGGCGTAGAAATGAAGATGCACATCCCAGCTTCA TCAGAGAGTCTCTGGCTGAGAAGCTGTCTAAGCTTGCTGACGGTGACAGGGCAACCACAGAAGAGCTGCT CGTTCTCTTGGAGGAAGGCGTGTGGGACAGCATTGAGCAGCAGGGCTTTAACAGGTTGTCCAGCGAATCC AGCAGCCAGTGGTCATTAGTGTTACAGTTCTGCATGCTCCATGATATGAAACTGAGCGTGTCCTACCTTA GAGAATGTGCCAAAGCAAATGATTGGCTGCAGTTTCTGGTTCACAGCCAGCTCCATAACTACCACCCAGA AGAGGTGGAAACCCTGCTTCAGTATTTCAGTCCTGTCCTTCAGAGTCACTTGAAGCTGGCTTCTGAGAAA CTGTCATCAGGGTCCATCTCTAAGGACGACAGCTGCCTCCAGGAACTCCAGAAAAACAAAGAAACAAGTG ATTTCTTTGAAATTCTCCACCGGTGCTCGGATGAGTCAGCTTCCTGGTGCTGGCTCCTGGCTGAAGCAGT GAGACATCGGGTTCCCATCCTCAGTGTCCTGGCCTCGTGTATCCAGGGTGCCAGTGCTGTCCCCTGTCTC TGTGTTTGGATTGTCACCTCTGTGGAGGACAAGGTTGCAGCTGAAGCAATAGAACACATTCAGATCTCTG TAGAGGACCATCATTGGAGCCTCGAGGACCTGTCCATTATCTGGAGAACTGTATTGACAAGACGGAAGAT CCACACTCTCATCCGAGGCTTCCGGCTTTTCATTAAGGATTCCCCACTTCTCTTGATTATGGAAATGTAT GAACTGTGTGTGTTCTTCAAGAACTACGGAAAAGCCAAAGTTAAGCTTGTGGAGTTCCAGAAAAGCCTTG AAATGCTTGACACAGTGGCTACTCGGGTCCTTCCTGTCATACCTGCCACGTGGATGAGGGAGCAGGTGTG CTTCCTTTTGAAACTTATGCCACAGCAGTGCGAGACACAGTATGAGCTGCAGAAAGTCCTCCAACTGTTT GTCGGGATGGAGCAGCTCTTCTCCGATGGGCCAGACGTGCAGAAGCTGTATCGGCTCAGCCAAGTACTGA AGGACACACCTATCACCATCAGCCCTGCAGTCATTTCCAGCTACAGCACTGAGAGTTTGCAGCGTGAGTG TAGATCTATTTTGGAGAAGCTAAAGGCAGATGGACAGTTTGCTGTGGCCAGGAGAGTGGCAGAATTAGCA TCCCTGCCTGTGGACGGCTTGGTGATTGAACAGTTAACGCAGGAAATGCAAATGCTAACACACACCCAGC AGTGGTCCCTGAAACAAGCAAGACTTGACTTCTGGAGAAAATGCCATGAAAATTTTAAGAAAAACTCTGT TTCAAACAGAGCAGCCTCTTCCTTCTTCTCCAGCCAAGCCCCGGAGGCGAGTGAGCACCCTGCTGAGCAG GGCAGCCTTGAGGAGCGCCATCTGCTGCTCACTCTGGCGGGCCACTGGCTTGCCCAAGAAGAGCCAGTTC CCGTGGAGGAACTGGAGGAGCTGGAGAAACAGATTTGGCTCTGCCGCATCGCCCAGCACACATGTGGAGG GGCTGAAGAAGAAACCAAGCCCAGCCTCTCGCAACACAAGTTGGCCCCTGCTGAACTTTCTTCTGATAGC TCAGCCAGTGAGTTCTCCTTCTCCAAGCTTGCTGCTTTGAACACATCCAAATACTTAGACCTTAATGGTC TTCCATCCAAGGAGATGTGTGAGAACACACTGGAACACAGAGAGCAGGAGTCACTGAACACTTTGATTGG GCAGCTGCTGGATGGTGGCTGTGTGCATGAAGCGAGCAGAGTGTGCCGGTATTTCCGTGTCTATAGTCAG GATCTAGTGCTGCTGCTGCACTGCAGAGCCTTGGCCTCCGCAGAGGCCAGCATGGAAGATCTGCACTCAG AGATCCGTGCCCTCCTCTCAAATGCCACACTACCTGAGGACCTGGAGAGCCCTGGCGTCCCTCTTCGGAA AGTCCATAGCAGAGAAAAGCAGCCTTTCAACCTAGCAGAGGAAAGCCAGACATTTCTTCAGCTAACTGCT TTGTGTCAAGACCGTACATTGGTAGGCATGAAGTTGTTGGACAAGATTCCATCTGTCCCCCATGGGGAGC TGTCATGCACCACAGAGCTCCTGATCCTGGCCCATCACTGCTTTACCTGTGCGTGTTACATGGAGGGCAT CATCAGAGTCCTACAGGCTGCCCGGATGCTTACAGATAACCACTTGGCTCCCAATGAGGAGTATGGATTG GTGGTACGCCTCCTCACTGGCATTGGACGGTACAATGAGATGACGTACATATTCGACTTGCTGCATCAAA AGCATTATTTTGAAGTTCTGATGAGGAAGAAGCTAGATCCAACCGGCACCCTGAAAACAGCCCTACTAGA CTACATCAAACGCTGCCACCCTGGAGACAGTGAAAAGCACAATATGATCGCCCTGTGCTTCAGCATGTGT CGGGAGATAGGAGAGAACCACGAGGCCGCAGCCTGCATCCAACTGAAGCTGATCGAATCTCAGCCCTGGG AGGAAAGTCTCAAGGATGGAGCCCAACTAAAACAGCTCCTGCTTAAAGCCCTGACTTTGATGCTGGACGC AGCAGAGAGTTACGCAAAGGACTCCTGTGTGCGGCAGGCCCTGCATTGTAACCGGCTCACCAAGTTGATA ACACTACAGATTCACTTTCTCAACAGTGGCCAGAACACAATGCTCATCAACTTGGGCCATCAGAAGCTAA TGGACTGTATCATGACCCTACCTCGGTTCTACCAGGCTTCTATTGTAGCTGAGGCCTATGACTTTGTTCC TGATTGGGCTGAAGTACTCTACCAACAAGTGATTCTTAAAGGAGACTTTAGTTACTTGGAAGAATTTAAG CAGCAGAAGTTACTAAAACCCAATATATTTGAAGACATTTCCAAAAAATATAAACAACACCAGCCTACTG ACAGGGTCACAGAAAACCTGAAGAAGTTACTTTCATATTGTGAAGACATCTACCTGTATTACAAGTTGGC ATATGAACACAAGTTTTTTGAAATTGTAAATATGCTTCTGAAGGATCCTCAAACAGGATGCTGTCTTAAA GATATGCTCGCGGGTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191571
Insert Size	6948 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191571.1, NP_001178500.1
RefSeq Size	7029 bp
RefSeq ORF	6948 bp
Locus ID	311372

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.