Frem1 (NM_001191757) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1306981
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217752 representing NM_001191757 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACTCTCCAGGCTGCACTGGGCCCAAGGCTCTGTTGTTCTTGCTGCTCCAGCTGCTTATGCTACACC TGGACCCAGCCAATGCCACCTTCATCAGCATTAACCGGGGCCTGAGGGTGATGAAGGGTAGTTCTGCCTT CCTGTCTGGAGATCACCTGAGGTTTGCCATCCCTAAAGAGAAGGACGCTTGCAGAGTGGAGGTGGTGATG AATGAACCCATAACCCAGAGGGTTGGGAAACTCATTCCACAGGTCTTTGATTGCCATTTTCTTCCCAACG AAGTACAATATGTTCACAATGGATGCCCAATTCTGGACGAAGACTCTGTGAAGCTTAGACTTTACAGGTT TACTGAAACAGAGACTTTCATGGAAACCTTCCTCTTGCGGGTCTCTCTTGTGGAACCAGACTGTAACATC ATCCGCCTGAGCAGCAACGTGTTGGAAGTGACTGAATTCTATGGCTTATCCCAAGCCATTGATAAGAACC TGCTCCAGTTTGATTATGATAGGACAGCCAGTCTGGACTGCACCATCAGAGTAGACCCCGTGAAGACTCA GCTGCCAGCCCATGGCAAGCTGGTTGTGGTGAATCGGAAGTCAGAGGGACCGCGTGGAGACCAGCCACAC AGCTTTTTCTCTGAGTCACAGCTAGGGGCAGAACGGAAGTGTCCGGATGGAAGCTGTACTCTGGAACCGA AGCAAGTGACAAGTCTGAAAGTGAGCTGTGAGGACTTCCTGCTGACAGGGTTTCACTACCAACACATGCA GCCCCCTTCACCCAACATTGACTACATCCCCATTCAGTTGGATCTCACAGACAGAAGGAGTAAAACCATT TACAAGTCAGAGAGCGCGTGGTTGCCTGTCTTCATCAGAGTTGGCATTCCTAACCAGGCCCCAAGGGCTG CTTTCATGGCCATGTTTGTTCTGGAGGTGGACCAGTTCATATTGACCCCTCTGACAACCTCCGTGCTGGA CTGTGAGGAGGATGAGACTCCGAAACCCTTGCTGGTGTTTAACATCACTGAAGCCCCACTCCAGGGCTAT GTGACCCACCTGCTGGATCATACCAGACCCATCTCTTCATTTACCTGGAAGGATCTCAGTGACATGCAAG TTGCCTACCAGCCACCGAACAACAGCCACCCTGAGAGGAGACATTACAAGATGGAGCTGGAGGTGTACGA CCTCTTCTTTGAGAGGAGCGCCCCCATCACAGTTCACATCTCCATCAGAACAGCAGACACGAATGCACCT CGGGTGTCCTGGAATACAGGTTTGAATCTCCTAGAGGGGCAGTCTCGGGCCATCACTTGGGAACAGTTTC AGATCGTGGACAATGATGACATTGGTGCTGTGCAGTTGGTCACCATTGGTGGTCTGCAGCATGGACGGCT TACAGTAAGAGAGGGGAAAGGATTTCTCTTCACCGTGGCTGATCTTCAGGCTGGAGTTGTTCGCTATCAT CATGATGACAGTGATACCACCAAAGACTTTGTGGCTTTCAGGATATTCGATGGCCAACACAGCAGCCATC ACAAGTTTCCTATCAACATCTTGCCTAAAGATGACAGTCCCCCATTCCTCATAACCAATGTCGTGATTGA ACTAGAAGAGGGGAAGACCATCTTGATCCAAGGGTCCATGCTGAGAGCTTCAGACATGGACTCCAGTGAT GACTACATCTTCTTCAATATCACAAAGTTCCCACAGGCTGGGGAGATCATGAAGAAGCCAGGGCCAAGAT TGATAGGATATCCTGTCTCTGGCTTCCTTCAGAGAGACCTGTTCAGTGGAATCATTTACTATCGTCACTT TGGTGGAGAAATCTTTGAAGATTCTTTTGAATTTGTCCTGTGGGACAGCCATGAACCTCCAAATCTCTCA GTGCCACAGGTGGTGATGGTCCATATAACTCCAGTAGACGACCAGCTTCCCAAGGAAGCTCCCGGCATAT CCCGGCATTTGATTGTCAAGGAAACGGAAGTGGCCTACATCACTAAAAAGCACCTACATTTCCTAGACAC GGAATCGCATGGTGGGGAGCTTGTCTACACAATAACACGCCCTCCATGCTTCTCCTCCAGCCACAGGCAC TTGGATGCTGGGAAGCTGTTCATGGTGGACAGCATACCAAAGCCAACCAAGAATCCCACAGCCCCAGGGC TGAACTCATTTACTCAGGTAAAAAGATTTGGAAAGACCCTGGCTAACACAGTAAGCACATTGTTGGGGGG CTTGGGCTTGGGTTGCTCCAACATTGTGATCTCTGTAAATCAGTGCATATGTGCATCTCAATTTGCTTCA CAGCATGCTGTGAACCACATGAAAGTGGCCTACATGCCACCCATGCAGGATATAGGTCCCACACCTCGAC ATGTGCAGTTCACAGTGTCCGTCAGTAACCAGCATGGAGGTACTTTGCATGGAATCTGCTTCAACATCAC AGTGCTCCCAGTGGACAATCAGGTTCCTGAGGTATTCACCAACACCCTGAGAGTGGCTGAGGGTGGCCAA TGCACCATCAGCACAGAGCACATTTTGGTTTCTGATGTGGACACCCCACTGGACAGCATCTGCCTCTCTC TTCAGGAGATGCCCCTGCATGGCGGGGTAGAGCTGGATGGATTTCCTCTAAACCCAAGAGGCACATTTTC TTGGCGAGACCTCCATACCTTAAAAGTTAGGTATCAACATGATGGATCTGAGGTTCTTCAGGATGAGATA TTCTTGGAGGTCACAGACGGCACAAATTCTGCAGGATTTGTTCTCTACATTGAGGTATTCCCTGTGAATG ATGAGCCACCTGTTCTAAAGGCTGACCTCATTCCCATGATGCACTGCTCAGAGGGTGGAGAGGTGACCAT CACCCCTGAATACATTTTCGCTACTGATGCAGACAGCAATGACTCAGAACTGCTATTTCTGATTACCCGA GAACCTCGGCATGGAGTAGTGAGAAAAGCTGGACTCCGTGTGGACAGGTTCTCTCAGGGAGATGTCATCT CAGGAGCTGTGGCATACAAACATACAGGTGGCGAGATTGGCCTCGAATCTTGTTTTGACACTGTTGTATT GGTGGTTTCGGATGGTGAAGCTGACCCTTTGGTGAATGGCTGTTGCTACAATGGACCCGATTCATCTGTT CCTCTTCATAAGTCCTTCCCCACATACCAGCTCAACATCACGGTGCATCCAGTCGACAATCAGCCACCTT CAATTATAATAGGTAGGACGCTCACGGTGGACGAAGGCTTCTCGGCAGCCCTTACGACTCATCACTTGAC TGCCACTGACTGGGACACTGCTCCGGATGACTTGGAATTCATTTTGGTTTCTCATCCCCAGTTTGGCTAC CTAGAAAACACACTGCCCTCTGCAGGCTTTGAGAAAAGGAATACGGGCATAAGCATAGCTTCATTCCAGT GGAGAGACATGAAAGCTTCGCATATTAACTATGTGCAGTCCAGGCACCTGAGGGAGGAGCCAACTGCTGA TGAGTTCCTGGTGTACGCCACAGATGGGAAGCAACGCTCCTTGGAGACCACATTTCACGTTATCATCAAC CCCACAAATGATGAAGCTCCTGATTTTGTGGTACAGAATATCACTGTGTACGAGGGCCACATGGTAGAGC TGGATTCTGCCATCATCAGTGCTACTGACAGGGATGTTCCCAAGGACCCCTTGCTCTTCAACATCACGCA CAAGCCCAAGCATGGCCTCCTCCTCATCTCGGCCACCAGCAAGGATGCTCATAATGAAATTCACAACTTC TCCATGGAACTGCTCCAGAATGGAATGAAGCTGGCGTATGCCCACGATGACTCGGAGAGCTCTGCTGACA ACTTCGTGATCCAGTTATCAGACGGGAAACATAAAATCCTTAAAACCGTTTCAGTAAGCATCACCCCAGT GAACGACGAGACACCAACACTAAGCAAGAAGGCTGAAGTCTCAGTGACTGTGGGCGACACTCGTGTTCTG TCTAGTGCTGTTCTTTCAGCCATAGATAAAGACTCACCCAGGGAGAAGATTCACTACTTATTTGAAAGAC TTCCCCAAAATGGGCAACTTCAACTTAAGATAGGGAGGGACTGGGTCCCTCTCTCCGCTGGCATGCAGTG CACCCAGGAGGATGTGGACTTGAACTTGCTAAGATACGCGCATGCTGGTAAAATGCATTCCCAGGATGGA GACCGCTTTACCTTCTACCTCTGGGACGGGGACAACAGGTCACCTGCATTTGACTGTCACATCATCATTG AAGACACAGGAAGAGGTGATATTGTCATTCATGCGAAACCACTCGTGGTAGCCAACGGTGACAGAGGTCT CTTGACGCCTGCCACTCTTCTGGCTGAGGATGGAGCAGACAAGGCTGAGGAATTACTCTACCTTATCACT TCCCCACCACAGTATGGCCAAGTGGAGTATGTCTACTCTCCTGGAGTCCCCATTACCAGCTTTAGCCAAA TGGACATAGCAGGACAGGCAGTCTGCTACATACACAAGAGCAGGACCGCTGTCCTCAGTGACAGTTTCAG ATTTACCATCAGCAATGGACTGCAGACCCAGCAGGGGGTGTTTGAGATCACACTGCAGGGTGTGGACAGT GCCTTGCCTGTGCTGACCAGGAACCGAAGGCTGAGGCTTGTGGAAGGGGCCATGGGCCTGCTGTCCACGG ATCACCTTCAGCTGACTGACCCTGACACACCTCCAGAGAACCTAACCTTCTTTCTGGTCCAGCTCCCGCG CCACGGGTACCTCTACCTGAGAGGGAAAGTACTTCAGCACAACTTCACCCAGCAAGATGTAGACAGCAGG GTTGTGGCCTACCAGCATGCAGGAGGCGGTGCCCAGGAGGACTGCTTTACTTTCCTGGCCACAGACAGGA AGAACCAAGGCTTCGTTGTGGATGGGAAAGTTCAGAAAGAGCCCGTTCTCTTCATTATCCAGGTGGACCA GCTGGATAAGAAGGCGCCTCGGATCACACGCTTGCACTCCCCTTCTCAAGTGGGGCTCTTGAAAAATGGC TGCTATGGGATTTACATCACTTCCCATGTGCTAAAGGCGTCTGACCCTGACACAGAGGATGACCAGATCA TCTTTAAGATTTTACGAGGCCCGTTGCATGGACGTCTGGAGAACACGACAACAGGTGAATTCATCCACGA GCAATTTAGCCAGAAGGACTTGAGCCGCAAGACCATCCTTTATATCATAAACCCTTCTTTGCAAGTGAAT TCAGATATCCTGGAATTTCAGGCCATGGACCCCACAGGGAACACTGCCCCTCCCCAGAGATTGGAACTGA AATGGTCATATATTGAATGGTCCCAGACTGAATATGAGGTCTGTGAGAATGTGGGCTTACTGACCTTGGA AGTCACCAGAAGGGGATATTCCATGGACTCAGCCTATGTGGACATAGAGGTTAACCAAGTGTCAGCTGTA GTTGGAAAAGATTTTACTGTCACTCCATCCAAACTGCTTCAATTTGACCCAGGAATGTCAACTAAGATGT GGAATATAGCAATTACCTATGACGGATTAGAGGAAGACGATGAGGTCTTTGAAGTAATTCTGAACTCCCC TGTGAATGCGGTTCTTGGCACACAGACAAAAGCAGCAGTGAAAATTTTGGACTCAAAAGGAGGACGGTGC CATCCTTCAAATTCCTTCAACCAAAGCAAGCACAGCACGTGGGGGAAGGGACGTTGGCATCCACTGCCCT CAGGGTCATCCTCACTCACCACTTCCGGTTCTCCTCATCTGGAAAGAAGGCCCCCTCCATCCATCACCAA AGGAGATGCTCTTCGGGGTTTTGATCTCACAGACCTCTCTCAAAGGAAGATGACGACACAGGGGAATGGC AAATCAGTCCTCCCATCCTCTGTTTGTAGAAATGGAACAGACACCATCTACAATTATCATGGTATAGTTT CCTTGAAACTGGAGGGGGACAGATTCTCAGCTCACAAACGGAAGGCCAAAATATCCGTCATTCGTCAGCC ACAGAGGACAGTCAAGGTGGCAGAGCTGCCTCTAGCCGATAAGGTAGAACCCACAACCGACTCGAACTTT CTCAGTCAGGGTCTACGGCCCTTGTTTCCAAAGAACTGTTCTGTGGAATTAAAGGGACTCTTTCATTTGG AAGAACACACCCACAGATTGTATCAATGTGATGGGATCGCCTGGAACGTCTGGAGCTCCCAAACCAAGGG GCTGGAGGACAGATCCTGCCCAGTTGGATGGCTTCTTCATTCCGACTACTGTCACGTCTTGGTCACAAGG CAGAAAGGCACCTGGGCCACAGCTACCCGAGCTTGCAGAGAACAGCACCAGGGCACCCTTGTGACCGTAT TCTCCAGGAGGCACATGCAGTGGCTGTGGGCTATCAGCGGAAGAAAGCCCTTCTGGATAGGCTTGAAGAA CCAAGTGCGAACTGGCCACTGGGAGTGGATTGGAGGTGAACCTGTTGCTTTCACCAACTGGAGGAGAGGG CCCCCTCTGCACCCGAAGCCTGGAAAGAACTGTGTTTTGGTTCAAAGAAGAGGACAATGGCAAACAAAGA ACTGTAGCAAAGGCAAGGCTCACAATTTTGTGTGCTCAAGGAAACTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191757
Insert Size	6630 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191757.1, NP_001178686.1
RefSeq Size	6630 bp
RefSeq ORF	6630 bp
Locus ID	298185

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