Espl1 (NM_001170602) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217737 representing NM_001170602 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGAACTTCAAAGGAGTCAAGTTCGCCACTCTACTTTGCTGCAAGAGAGAGACCCAGGAGTTGCTGC CTGACTTGAAGGAGTTCCTGTCTAGATCTCGAACTGATTCAGTCAGCAGCAGAACCGATGCTGAGAGAAG ACAGGTCTGTGACACCATCCTGAGGGCTTGCACCCAGCAGCTGACTGCCAAGCTTGACGGCCCTGGTCAC CTGAGAAGTATCCTGGACCTGGCAGAGCTGGCCTGTGACGGCTACTTGTTGTCTACCCCACAGCGCCCTC CCCTCTATCTGGAACGCATTCTTTTTATCTTACTTCGGAATGGTTTGACTCAGGGAAACCCAGACGCTGT GCTCCGCCTTGCCCAGCCTCTCCATGCCTGCTTGGTACAGAACTCTCGAGAGGCTGCCCCTCAGGACTAT GAGGCTGTGACCCGGGGTACTTTTTCTCTGCTTTGGAAGGGAGCAGAAGCCCTGTCAGAGCGGCGAGCTG CATTCTCAGCTAGGCTGAAGGCCTTGAGTTTCTTAGTGCTTTTGGAGGATGGCAGCCTCCCTTGTGAGGT CCCTCACTTTGCTTCTCCAACAGCCTGTCGGGTAGTTGCCGCCTATCAGCTGTTTGATGCTACTGGACAA GGTCTAGATGCAGCTGACGCTGACTTCGTCTATGACTTGCTTTCTAGGCATGTGATCAGAGTCTTAGTAG GCGAGGGTGGTAGGGCTTCTGGTCCGCTCTCTCCTCAGAGGGCCCTCTGCCTCTTGGAACTCACCTTGGA ACATTGTCGTCGACTCTGCTGGAACCAGCACCACCACAAGGCTACCATAGCAGTGGAGAAGACTCGAGAT CACCTAGGAAATAGCAATGTAGCTCCCAGCCTCCAACTGTGCCAGATGGGGGTTGAGCTGCTGGAAGCTG TGGAAGAAAGACCAGGGGCAGTGGCCCAGCTCCTGATGAAGGCAGCTGCTATTCTGAACAGCAGTATGGA TGGGCCATCACCCCCGCTCCGGGCATTGTATGACAGCTGCCAGTTCTTCCTTTCAGGCCTGGAGAGAACC ATCAAGAGGCACTATGGAGTTGATGCCATATTGAATCTCTTTGCTTTTCTTGGGGGATACCACTCCCTTG TCCGGCACCTGCGAGAAGCCTCCGAAGCGTCCTCTAAGCAGCAGCAGAGCCTGCTCCAGATGCACTTCCA TACCCTCCACCTCTTCACTGGGATTGTGTATGACTTTGCCCAAGGCTGTCAGGTAGCTGAGCTTGCTCAG CTAGTGGACAGTTGCAGATCTGCTGTTGTCTGGATGTTGGAGGCCTTAGAGGGCCTGTCTGGTGGAGAGC TGGCTGACTACCTGGGCGTGACTGCCTCTTACACCAGCAACTTGGCCTACAGCTTCTTTAGTCAGAAGCT CTATGAGGAGGCTTGTGCCATCTCAGAGCCAGTCTGTCAGCACTTGGGCTTGGCTAAGTCAGGCGCTTGT CCCGAAGTGCCTCCTGAGAAGCTACATAGGTGCTTCCGGCTGCATGTGGAGAGTTTGAAGAAATGGGGTA AACAGGCCCAGGGCTGCAAGATGGTGACCTTATGGCTGGCAGCTCTGAAACCCTACGGTCTCCAACACAT GGTTGAGCCAGTCACTTTCTGGGTTCGGGTCAAAATGGATGCAGCCAAGGCAGGAGACAAGGAGCTACAA CTGCAGACTTTGCGAGACAGCCTAAGTTGCTGGGACCCAGAGATGCAGTCTCTGCTACTGAGGGAGGAGC TGCAGGCCTATAAGGCAGTACGGGCTGACACTGGGCAGGAACGCTTCAACGTCATCTGCGATCTGCTTGA GCTCAGCCCTGAGGACACAGCAGCTGGAGCCTGGGCACGAGCTACCCACCTGGTGGAATTGGCCCAAGTG CTGTGCTACCATAGCTTTGCCCAGCAGACAAACTGCTCTGCCTTGGATGCTATCCAGGAGGCCCTGCAGC TTCTGGAGTCCGTGAGTCCCGAGGCACAGGAGCAGGACCGCCTTCTAGATGACAAAGCACAGGCCCTGCT ATGGCTCTACATCTGCACCTTGGAGGCCAAAATGCAGGAAGGTATCGAGCGGGATCGGAGGGCCCAGGCC CCTAGTAACTTGGAGGAATTTGAAGTCAATGACCTGAACTATGAAGATAAACTGCAGGAAGATCGCTTTC TATACAGTAACATTGCCTTCAATCTGGCTGCTGATGCAGCGCAGTCCAGATGCCTGGACCAGGCCCTGAC TCTGTGGAAGGAAGTGCTCACCAAGGAGCCAGCCCCAGCTGTGCGGTGTCTCCAGCAGACGGCAGCCTCC CTGCAAATCCTGGTGGCCATCTATCAGTTGGTGGCAAAGCCCTTGCAGGCTCTGGAGACCCTCCTGCTCC TTCAGATTGTATCTAAGAGACTACAGGATCATGCAAAGGCAGTCAGCTCTTCCTGCCAGCTTACCCAGCT ACTCTTGAACCTTGGCTGTCCCAGCTATGCCCAGTTGTATCTGGAAGAGGCAGAGTCAAGCCTGAAGAAT CTGGACCAAGCGACTGACACGTGCCAGCTGCTTTCCCTGACCTGTGCTCTGCTTCGAAGCCAGCTCTGCT GGGCTTGCCAGAAGGTGACTGAGGGCGTTTCTCTGTTGCTTTCTGTCCTTCGAGATCCTGCCCTCCAGAA GTCATCCAAGGCTTGGTACTTGCTACGTGTCCAGGCCCTGCAAATTCTGGCTTTCTACCTCAGCCTCTCG TCCAACCTCCTCTCAAGTGCCCTACGAGAGGAGCTCTGGGATCAAGGCTGGCAGACCCCAGAGACAGCAC TGATAGATGCCCACAAGCTTCTGAGAAGCATCATCATCTTGCTGATGGGTGGCGATGTGCTCTCCGTTCA GAAAGCAGCTGTGGAGTCGCCCTTTCTGGATTACGGTGAGAATCTGGTGCAGAAATGGCAAGTTCTCACA GAGGTGCTGACCTGCTCGGAGAGGCTGGTCAGCCGCCTGGGCCGCCTGGGGAACGTGAGTGAAGCCAAGG CCTTTTGCTTGGAGGCCCTAAAACTTACCACCAAGCTGCAGATACCTCGCCAGTGTGCCCTGTTCCTTGT ACTGAAGGGTGAGCTGGAGCTGGCCCGGGGAGACACTGACCTCTGTCAGTCAGACTTGCAGCAGGTTCTG TTCTTGCTCGAGTCTTCCACAGAGTTTGGTGTGGTGACACAGCACCCAGACTCAGTGAAGAGGGTGCACA CGCAGAAAGGGACGCATAAGGCTCAAGTCCCCTGTTCCCCACCTCTACCAGAAGAGGAACCCTTCCTGAG AGGCCCTGCCCTTGAGCTGGTGGCCACTGTGCTCAAGGAGCCCGGTCCCATCCAACCCTCTGGAAACTCC TCCCCGGTCCTCAAGACCAAGGCATCACCCAAGCCTGGCTTCCTGTCTCACTTGCCCAGCTGTGATTGCT TACTGTGTGCCAGTCCTGCCCTCTCCACCGTCTGTCTGCGCTGGGTGTTGGTCACTGCAGGAGTGAGGCT CACCACAGGTCATAAAGCTCAGGGTCTGGATCTGCTGCAAGCTGTGCTGAAGGGTTGCCCTGCAGCCACC AAGCGCTTCACACAGAATCTTCAAACTTCTCTGAATCACAAAGCACCCCCCTCTTGTGTTCCAAGCCTCT TTGATGAGATCATGGCTCAAGTGTACACACACTTGGCATTAGAGTGCCTGAATCAGACATCTGAAAAGAG CCTGGGAAAGGTCCTGGCGTCAGGACTGAAGTTTGTGGCAACACGGATACAATACTTAGAGTTCTGGCAA GCCAGCCTGCTCTTGGTTCAGGCCCTTGCAAAGCTGGCCCGCTTCAGCTGCTGTACTAGCGAACTCTTTG CAAGCGCCTGGGGCTGGCAGCCACCATTAGTAAAAATCCCGCCAGTCCTTGAGCCTTCTAAGATTCGGAG ACAAAAATGCTCTGGACAGGGGCGCCAGCGGGTAGCCTCTGCCCCCTCACCCCTCCATAGCAGCTCTCAG GAAGGTCTGGAAGAAGGGCCACCTTGTACACCTAAGCCCCCAGGCCGTGCTAGGCAAGCCGGCCCTCGTG TCCCTTTCACCATATTTGAAGACATCCACCCCACAAAGAGCAAGCTTAAGGTTCCCTTGGCTCCCAGGGT CCACAGGCGGGCCCAGACTCGACTCAAGGTGAACTTCAGCGATGACAGCGACCTGGAAGACCTTGTCTCA GCTGAAACACAACTTGTGGAGGAACCTAAGAGGCGAGGGACTGCTTCCCGCACCCGGGGTCAGAGTAGGA AGGGCCCTAGCTTAAAGACAGATGCAGCGGTTGCCGTAGGTAGTACCCCTGGACATCCCAGTCTCAGTGG GAGGACTCGGAGGGCCAGGAAGGTGGCTTCAAGACATTGTGAAGGTGTCGGGGCCAGCCTGGGACCCGAA ATCATGAGAAGCATCCCTGAAGAGGAGCTGATGGACAGCCAGCTAGAAAAGAGCTTTGAGATTCTCAGGG CTTCTGATGGGGAAGACTCAGCCTCAGGCGAGAAGGCAGCAGCTGCAGAGCCTGGTCTACCCATAGGAGA GCGTGAGGTGTTGAGACGGGACTCCAGCAAGGCAGAGCGACCTGTCTTGTACCCTGATACAGAGGCCGAT AGTGACCTTAGTCCTTGGCTCCCACCCTTCTCAATTCCTGCACCCATTGATCTTTCTACCCTGGATTCCA TCGCTGACTCATTGAGCATTGCTTTTCGTGGTATTAGTCACTGCCCTCCAAGTGGGCTCTACGCTCACCT CTGCCGCTTTTTGGCCTTGTGTCTGGGCCACCGGGATCCCTATGCCACTGCGTTCCTTGTAGCAGAGTCT GTCTCCATCACCTGCCGTCACCAGCTACTCACCCATTTGCACCGACAGCTCAGCAAGGCCCAGAAGCAGC AAGGCTCACCTGAACTAGCAGAGCATCTACAGAGGCTGGACTTGAAGGAGAGGCCCGGAGGTGTCCCACT GGCCCGCGTGCAGCACCTCTTTTCCTTCAGGGCTTTGGGATCTGGCTGCTTCCCCCAGGCAGAGAAGGAC AGTTTCCAGAAGCGCCTGGCTCTCATCCCCAGTGGGGTGACTGTCTGCGTGTTGGCCCTGGCCACCCTCC AGCCTGGAACCTTGAGCAACACTCTCTTACTGACCCGCCTGGAAAAGGACAACCCCCCAATTACTGTGAA GATCCCCACTGCCCAAAGTAAGTTCCCACTAAGTGCGGTGCTGAAAGAGTTCGATGCCATCCAGAAGGAC CAGAAAGAGAACAGCGGCTGTACTGAGAAACGAGCGTGGTGGACGGGCCGGCTAGCACTAGACCAAAGGA TGGAGGAACTCATTACCTCCCTAGAGGAGCATGTGCTGGGCTGCTGGAGGGGGCTGCTGCTGCCGTGTAG CACCGACCCTGGCCTTTCCCAGGAGGCCTCCCAACTACAAGAGTTGCTGCACGAATGTGGCTGGGAATAT CCTGATTACACTCTCCTAAAAGTCATTCTTAGTGGGGCCAGAATCCTCTCCAGCCAGGATGTTCAGGCCT TGGCATGTGGGCTGTGCCCAGCCCAACCTGAGCGAGCCCAAGTGCTCCTGAGCGAGGCAGTAGGACGGGT ACAGAGCCAGGCAGCCCCTCCGAGTCAACATCTTGTACTGGTGTTAGACAAGGACCTGCAGAAGCTGCCG TGGGAAAGCATGCCATGCCTCCGAGCACTGCCGGTCACCCGGCTGCCCTCCCTCCGCTTCCTGCTCAGCT ACTCAATCGCTAAGGAGGCTGGGGCCTCATCAGTGCTGAGCCAAGGTGTTGATCCACGCAATACCTTCTA TGTGCTAAACCCTCACAGTAACCTGTCGAGCACGGAGGAGAGATTTCGAGCCAGTTTTAGCAGTGAAGCC GGATGGAAAGGCGTGATTGGGGAAGTGCCCAGCCTTGAGCAGGTGCAGGCTGCCCTGACAGAGCATGATT TATATATCTATGCAGGACATGGAGCTGGTGCCCGCTTCCTTGATGGGCAGGCTGTCCTGCGGCTGAACTG CCGGGCAGTGGCCCTACTGTTCGGCTGCAGCAGTGCAGCCCTCGCAGTGCATGGAAACCTGGAGGGAGCT GGTATTGTGCTCAAGTACATCATGGCTGGCTGCCCCTTGTTCCTAGGTAATCTCTGGGATGTGACTGACC GTGATATTGACCGTTACACAGAGGCTTTGCTGCAGGGCTGGCTGGGAGCAGGCCCAGGGGCTCCCCTTCT CCACTATGTCAGCCAAGCCCGCCAGGCCCCACGACTCAAGTATCTTATTGGTGCTGCACCTGTAGCCTAC GGCCTACCTGTCTCTCTACAAACATCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001170602
Insert Size	6330 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001170602.1, NP_001164073.1
RefSeq Size	6835 bp
RefSeq ORF	6330 bp
Locus ID	315330

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