Ralgapa1 (NM_020083) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Garnl1; GRIPE; p240; Tulip1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217729 representing NM_020083 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCTCTAAGAAGCCGCACGGGGACGTGAAGAAGTCCACCCAGAAGGTGCTGGACACCAAGAAGGACG CGCTGACCCGCCTCAAGCACCTGCGCATCGTCATCGAGAATGCAGACTCTATTGACCTTAAACAGTTCTT TGACCAGCATTTTTCACATATATACTATGTGTTTTTTGAAAATTTTGTGACTATTGAAGCCAGTCTTAAA CAGAAAGGTCACAAGTCTCAAAGAGAGGAATTAGATGCTATACTTTTTATTTTTGAGAAAATTTTACAAC TTCTTCCAGAAAGAATTCATCAGCGATGGCAATTCCATAGTATTGGGTTGATTTTAAAGAAACTACTGCA CACAGGAAACTCCTTAAAGATCAGACGGGAAGGTGTTCGTCTCTTCTTGTTATGGTTGCAAGCTCTTCAG GATAACTGTAGCAAAGAGCAGCTCTGGATGTTTTCATGCTTGATCCCTGGATTTTCAGCACCACAATCTG AATATGGACCTCGAACTTTAGATAATCTTATTAATCCTCCACTCAACCTTCAAGAAACTCAAGTAACTAT AGAGGAAGTCACCCCTCTTGTTCCCCCGCAGTCAGGAGACAAAGGACAAGAAGACCTCACAAGCTATTTT CTTGAAGCACTTTTAAAATACATAGTAATTCAGGTAAAAAGTTTAGAATGGAAGAACAAAGAAAACCAAG AAAGGGGATTTTCATTTTTGTTTTCACATTTTAAGAAGTTTTACTTGCCTTATATTTTCCCAAACCTCTG CAAGGAGAATAGTTTGTATCATCCTGTACTTGATATCCCACAGGTCAGACCAAAGCCTCATTATGTGATG GTAAAGAAGGATGCTGAAACCAATGAAACGATATACTGTACAAAAGAGCCTTTCATCCAGGCTCGGGTCA TTGTCATTCGTTGGCTGGTTTCTTTCTGGCTGGAGCCAAAACCGCATTCAGGACCTAATATTCCTGGGAT GGAGGGTGAAGTCTTGCCAAAGAATATTCAGAGAGCAGCTGCTAGTTTGGTCTCCAGAGAAGAAAGCAAA AATGATACTGTTGATAAAGCAGACAAAACCGCAGAGCCCGAGCAGTCGCATTCCAATACAAGCACTCTCA CAGAGCGAGAGCCCAGCTCTTCCAGTCTCTGTAGCATCGATGAAGAACACCTCACGGACATTGAAATCGT TCGCAGGGTATTTTCATCTAAGAGAAGCAATGTAAACTTTGTCACAGAGATATTTCGTCAGGCATTTTTA TTACCAATTTGTGAAGCAGCAGCTATGAGAAAAGTGGTAAAAGTGTATCAAGAGTGGATTCAACAGGAGG GAAAACCTTTGTTTATGCAAGAGCCTGAAGAAACTGTGATCACTTCTTCAGATATACCTTGCAGTGAAAA TGTCACAGACCATGACATTTCAATAGAAGACGGAGAGAAGAGAGAAGAGGAAAATGGGACCAATATTTCT GAACATGTTCGAAATTCTACCTGGACAAAGAATGGTTCCTACCAGGAAGCTTTTCATGTCTCTGAAGAAG CCACAGAACAAAATATTCAAGCTGGTACTCAGGCTGTTTTACAGGTGTTCATTATAAACTCATCAAACAT ATTTCTTCTTGAACCTGCAAATGAAATAAAAAATCTTCTGGATGAGCACACAGATATGTGTAAACGTATT CTTAACATATATCGATACATGGTTGTGCAAGTATCAATGGACAAAAAGACCTGGGAACAGATGCTACTCG TGTTGCTCAGAGTCACGGAGTCTGTACTGAAGATGTCGTCACAAGCTTTTCTGCAGTTCCAGGGGAAAAA AAACATGACCCTGGCAGGTCGACTTGCAGGACCACTTTTCCAGACTCTCATTGTTGCCTGGATCAAAGCA AATCTAAATGTATACATTTCTCGAGAACTTTGGGATGACTTACTTTCAGTATTGTCATCGTTGACCTATT GGGAAGAATTGGCCACTGAGTGGTCGCTGACCATGGAGACACTAACTAAAGTTTTAGCTAGGAATTTATA TAGTTTGGATCTCAGTGATTTGCCATTGGATAAGCTCAGTGAACAGAAACAAAAAAAGCACAAAGGGAAA GGAGTTGGACATGAATTTCAGAAAGTTTCAGTTGACAAGTCATTTTCTAGAGGATGGAGTCGCGATCAGC CTGGCCAGGCACCCATGAGACAGAGAAGTGCAACGACTACTGGCTCCCCGGGAACTGAGAAGGCCAGGAG TATAGTACGGCAAAAAACTGTTGATATTGATGATTCTCAAATCCTTCCCCGCTCAACCAGAGTCAGACAT TTTTCACAGAGTGAAGACACTGGTAATGAAGTTTTTGGTGCTTTGCATGAGGAACAGCCACTGCCTCGAA GTAGCAGCACTTCTGACATCTTGGAACCATTCACTGTTGAGCGAGCCAAAGTAAATAAAGAGGACACGAG CCCCAAACTGCCTCCTCTTAATAGTGAGACTGGCGGCAGCAGCGCTAATGTTCCTGACCTGATGGATGAG TTTATCGCAGAGCGACTTCGAAGTGGCAATGCCTCGACTATGACAAGAAGGGGAAGTAGTCCAGGCAGCC TTGAAATTCCGAAAGACCTTCCTGATATTCTAAACAAGCAGAATCAGATGCGCCCTGTTGATGACCCTGG TGTGCCCTCAGAATGGACGTCTCCTGCCAGTGCAGGGAGCAGTGATCTCATGAGCTCAGATAGTCACTCG GATTCCTTCAGTGCCTTCCAGTGTGAGGGCCGAAAATTTGACAACTTTGGCTTCGGGACTGACATTGGGA TCCCATCTTCTGCTGATGTGGATTCAGGCTCTGGCCATCATCAAAGCACTGAGGAGCAGGAAGTGGCTAG CCTAACGACGCTTCATTTAGACTCAGAGACAAGCAGCCTTAATCAACAAGCATTCTCTGCTGAAGTTGCA ACTGTCACTGGTTCAGAAAGTGCTTCCCCTGTTCATTCAGCTCTGGGGTCCAGGTCACAGACACCTTCCC CTTCTACGCTGAATATAGATCACATGGAACAGAAGGATCTACAGCTTGATGAGAAGCTCCACCACTCTGT TCTTCAGACGCCAGATGACCTAGAAATCAGTGAATTTCCTTCTGAATGTTGTAGTGTGATGGCAGGAGGT ACGCTCACTGGGTGGCATGCTGATGTTGCTACTGTTATGTGGCGAAGAATGCTTGGCATTTTGGGAGATG TCAATGCTATTATGGATCCTGAAATACATGCTCAAGTTTTTGATTACCTCTGTGAACTTTGGCAGAATCT AGCCAAGATCAGAGATAATCTTGGCATTTCAGCCGATAACCTGACTTCACCTTCTCCACCTGTTTTGATT CCTCCACTAAGAATTCTTACACCTTGGCTTTTTAAGGCAACCATGTTGACTGATAAGTATAAACAAGGTA AATTACATGCATATAAACTTATTTGTAATACAATGAAGAGAAGACAAGATGTATCCCCAAATAGAGATTT TTTAACGCATTTCTACAATATAATGCATTGTGGATTGCTTCATATTGACCAGGACATCGTCAATACGATC ATCAAGCACTGCTCACCTCAGTTTTTTTCACTTGGTTTGCCTGGGGCCACAATGCTTATTATGGATTTTA TTATAGCGGCTGGGAGAGTGGCTTCTTCAGCTTTTCTTAATGCACCAAGAGTGGAAGCACAGGTTCTTCT GGGATCTTTGGTTTGCTTCCCCAACTTATACTGTGAGCTGCCTGCTCTGCATCCAAGCACGCCTGACATC GCTGTGTCTCAGTTCACAGACGTCAAGGAACTTATAATTAAAACTGTCTTAAGCTCGGCAAGAGACGAAC CCTCTGGCCCTGCACGATGTGTAGCACTTTGCAGTTTAGGTATATGGATTTGTGAAGAATTAGTCCACGA ATCTCATCATCCTCAAATTAAAGAAGCACTGAATGTAATTTGTGTTTCATTAAAGTTTACTAATAAAACA GTTGCCCATGTAGCTTGTAACATGCTTCACATGCTGGTTCATTATGCACCTAGACTTCAGACTTACCAGC CTGACTCTCCCTTGAAAATTATTCAGATCCTAATAGCAACTATCACACACCTTTTGCCAAGTACAGAAGC TTCATCATATGAAATGGACAAGAGGTTGGTTGTGTCATTACTTCTTTGCCTTCTGGACTGGATCATGGCC TTACCTTTAAAGACAATACTCCAATCAGTCCATTCTACAGGAGCAGAAAATGAGAAGACAGAAAAATCTG TCCTCAACTGTATTTATAAGGTTTTACATGGGTGTGTATATGGAGCACAAAGTTTTAGCCATCCAAAGTA CTTTCCAATAAGCCTTTCTGATTTGGCATCTGTAGATTATGACCCTTTTATGCATTTGGAAAGTCTGAGA GAGCCTGAACCTCTACACTCTCCTGATTCAGAACGGTCTTCTAAACTGCAGCCGGTGACAGAAGTGAAAA CTCAGATGCAACAAGGATTAATTTCTATAGCAGCCCGTACTGTTATTACCCATCTGGTGAATCACCTGGG CCATTATCCAATGAGTGGTGGTCCTGCTATGCTAACGAGTCAGGTGTGTGAGAATCATGACAATCATTAT AGCGAAAGTACTGAACTTTCTCCTGAGCTCTTTGACAGTCCAAATATTCAGTTCTTTGTGCTGAATAATA CAACCTTAGTGTCTTGTATCCAGATAAGATCAGAAGAGAGTGTGCCTGGAGGAGGTTTAGCTGCTGGCCT TGTGTCAGCCAACTCAAATGTTAGAATCATAGTACGTGATCTCTCTGGAAAGTACTCATGGGATTCTGCC ATACTGTATGGGCCACCCATTGTAAGTGGTTTGCCTGAACCTACATCTTTCATACTTTCAATGTCTTACC AAGAGAAACCAGAAGAGCCTCCTACATCTAATGAATGCTTAGAAGACATAACAGTAAAAGATGGACTTTC TCTCCAGCTTAGAAGATTTAGAGAAACTGTTCCAACTTGGTCTACAATAAGAGAGGAGGAAGATGTTCTT GATGAGCTCCTGCAGTATTTAGGCACTACAAGTCCTGAATGTTTACAGAGAACTGGAATCTCCCTCAATG TTCCTGCTCCACAACCTGTGTGCATTTCTGAAAAACAGGAGAATGATGTCATTAATGCTATCCTTAAGCA ATACACAGAAGAAAAAGAGTTTGTAGAAAAGCACTTTAATGACTTAAACATGAAAGCCTCAGAACAAGAT GAACCAACACCTCAGAAACCTCAGTCAGCATTTTATTACTGCAGATTGCTTCTTAGTATATTGGGAATGA ATTCCTGGGACAAACGGAGGAGCTTTCATCTCCTGAAGAAAAATGAAAAGCTGCTCAGAGAACTGAGGAA CTTGGATTCAAGGCAGTGCCGAGAAACACACAAGATTGCAGTATTTTACGTTGCTGAAGGACAAGAAGAT AAATACTCCATTCTCACCAATATAGGAGGAAGCCAAGCATATGAAGATTTTGTTGCTGGCCTTGGTTGGG AGGTAAACCTTACAAATCACTGTGGCTTCATGGGAGGACTGCAGAAGAACAAAAGCACTGGACTGACCAC TCCATATTTTGCTACCTCTACAGTAGAAGTAATATTTCATGTATCTACAAGAATGCCTTCTGAATCTGAT GACTCTCTGACCAAAAAATTGAGACATCTAGGAAATGATGAAGTACACATTGTTTGGTCTGAACATACTA GAGACTACAGGAGAGGAATTATTCCTACAGAGTTTGGTGATGTCCTCATTGTAATATATCCAATGAAAAA CCACATGTTCAGTATCCAGATCATGAAAAAGCCAGAGGTTCCCTTCTTTGGTCCCCTTTTTGATGGTGCT ATTGTGAATGGAAAAGTTCTGCCCATTATGGTTCGATCCACAGCAATAAATGCTAGCCGTGCTCTGAAAT CACTGATTCCATTGTATCAAAACTTCTACGAGGAGAGAGCACGGTACCTGCAGACAATTGTCCAGCACCA CTTAGAGCCAACGACATTTGAAGACTTTGCAGCCCAGGTTTTCTCTCCAGCTCCCTACCATCATTTCCCT GCTGATGCAGATCATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_020083
Insert Size	6108 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_020083.3, NP_064468.3
RefSeq Size	7476 bp
RefSeq ORF	6108 bp
Locus ID	56785
UniProt ID	O55007
Gene Summary	mouse homolog interacts with the HLH region of E12 and may be involved in neuronal differentiation [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.