Thada (NM_001191769) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1560519
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217712 representing NM_001191769 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGTGAAGAAGAAAAGAGAAATGCAGTTTGCTGCACTGACTGTCTGTCATCAGGACCTGGAAACTC TGAGATCTTTTGCTGATGTGGAAGGGAAAAATCTAGCCTCTTTGCTGTTACATTGTGTACAGCTCACAGA TGGGGTGTCACAAATCCACTCTGTTAAACAGATTGTGCCTCTCCTGGAGAAGGTCGATAAAAACGGTGTG TGCGATCCCATTATTCAACGTTGTTTGGATATTTTAGCAAGCATTTATTTTTCCTTGACTCTGAAGAACC CCTTGAAGAAAGTTTTGGCAAGCTCACTCAATGGCCTACCTGAATGTTTTCTAAATGAGGCTGCACACAG TTTTACTTTTCATCTTCAAGAAGAATTAGATACTGCTGACTTACATTCTTATAGAAAAGTAATGGATAAC ATATCTTCCTGCATGGAGAACTTTAACCTGGGTAGAGCAAGTGTTGTCAACCTGCTTCAAAACGTGCTTC ATTTTCTGCAGAAGAGTCTAATTGAAGTCCAGGAAGAAAACAGAAAATTTGCTGGAAATCATATTGTTCA AACACAGTTAATGAATGACTTGCTGGTAGGTGTTAGAGTTGCCATGACATTGGTACAGAAAGTACAAGGG CCCCAGAGCAACCTTTGGAATGATTCCAGCTCTCCCATCTGGCAAAGTATGTGTGGCTTGCTGAGCATTT TCACCAGGTTTTTAAATGATGATGACCTGCTGCAGACTATTCAGAGTACGTCTGGATTAGCTGTTATTCT TTTCATTAAGACCATGTTCCGACCGTCTGAGAAGCTGCCTGGGTTGATCAGCAGCTTGCTTCTCCAGTCA GCTGACTGCACCAGCATCCCTGATTGGCTCATGAGCAGCTGTAGGAGCCTCTGCTGTGCTGATGTGCCAG CCTCAGCTCTCCTGTTCCTGTGTCAGGGAACACTTGCCATGTTGGACTGGCAGGATGGCAGCATGGGCCC AAGCAGGGAGGCTTTGCTCTTGGATACTGTGCATGTTTTGTTCACCTTGAGTTCACAGATTAAGGAGTCA ACACTAGAAATGTTCCTGTCTAGAATCTTGGCATCTTGGACCAATTCAGCCATACAAATCCTTGAATCAA GTTCCCCAAGCCTAAAGGATCATCTGAATGGGGATTCAAGCATACCTAGGAGACTTTTAGAATATGTCTA TACACACTGGGAACATCCATTGGATGCTCTGAGACACCAAACCAAAATCATATTCAGAAATCTTTTGCAA ATGCACCGGCTCACTATAGAGGGGGCAGATTTAGTCACTGACCCCTTCTTTCTGGAACTGACTAAGAGTC TTCTGCAGTTGGAATGGCATATTAAAGGGAAGTACGCTTGCCTTGGCTATTTGGTTGAGAGTCTTGGAAT TGAACATATTTTGGCAATAGATAAAACTATTCCATCTCAAATCCTAGAGGTGATGGGAGACCAGTCATTG GTACCTTATGCAAGTGACCTCTTGGAAACCATGTTTAAAAATCATAAGAGCCATTTGAAGTCCCAAACTG TCACCAATACCTGGATGGATAAGTGGCATGAGACTTGGGTTTTTCCTGTCCTTTCTGTACTGTGTGGAGG AAACTTGGATCAGAGGTCTTATGTGATTGATTATTACTTGCCGAGGTTACTGAATTACAGCCCCGAGAGC CTACACTACATGGTGCAGATTCTTCAGACGCCTATTGATACGGAAACTGGGTCGTGTAATCACAGAGGGG CTTTGGGAGCTTTGATGGCATGTCTACGAACAGCTAGAGCTCATGGACATCTTCAGTCTGCAACTCATGC CTGGGAGAACCTTGTGTGTAGTGCCAGGGTAAAGCAAGGCTTAATTCACCAACACTGCCAAGTGCGGATA GACACACTAGGCTTGCTTTGTGAAAGCAGTCGGAGCACAGAAGTGGTCTCCAGAGAAGAAATGCAATGGG TTCAGTTCTTCCTCACGTACAATCTCAACAGCCAGTCTCCAGGGGTGCGGCAGCAGATTTGCTCGCTCCT TACAAAGTTGTTTTGTAGGATACAAGAAAGTTCTCAGGTACTGTATAAATTAGAGCAAAGGAACTCCACA CCAGACTCTGAGAAGCGGTCCACCAGAGAGCAGCCTTCTGTCTCTTTACAGCAGTATAAGAATTTCATGT CATCTGTTTGCAACATTCTTTTTGAAGCATTGTTTCCTGGATCTTCCTACTCAACTAGATTTTCAGCTTT AACCATTTTAGGATCAATAGCTGAAGTGTTTCCTGCTTCAAAAGGTAACATCCAAACAGTGTATCAGCTG AGTCATGATATCGACGCTGGCCGTTTCCAAATACTTATGGAGTGCTTTACTAGTACTTTTGAAGAAGTGA AAACCTTAGCATTTGATCTTCTGATGAATCTGTCATCAGTAACCGCTGGGCAGCTCCAGGGTTCTGAGAA GCTGCAAGACCTGTTCCAGGCAGCGCTGGAGCTCAGCACCAGCACCAAACCTTATGACTGTGTGACAGCC TCCTACCTGCTGAACCTGCTGATCTGGCAGGATGCTCTGCCAGCATCCCTGTCTGCCTCTTCAGCACAGC AGCCCACATGTGGAGCTGGAGAGAAGTCTGCTGTGGTGGAAAGGAACACATTAGTGGTTATTAAATGCTT GATGGAAAACCTTGAGGATGAAATATCTCAAGCTGAGAACTCATTGCTTCAGGCTGCGTCTTCGTTTCCA ATGTATGGGAGAGTCCACTGTATAACAAGAGCGTTTCAGAAGTTGCCTCTACATGACCTGAGGCTCGTGA GTGAGTGGAAGCCAGTCCTGGGGAAGCTTCTGCTGCTGTCCTACAGGCTTTCCACTGTGGTATCTCCTGT CATCCAGAGCTCCTCCCCTGAAGGCCTCATCCCTATGGATACCGACTCAGAGTCAGCAAGCCGCTTGCAA GTCATTCTCAATGAGATTCAACCACGGGATACTAACGATTACTTCAGCCAAGCTAAAATACTGAAAGAAT GTGATAGCTTTGATTTGGAGGACTTGAGTACCAGTGTCTCAAATATTGATAGTTCTGCAGAAATCAAAGG TAAAGAAGAAAAAGCGTGTGATGTAACTGCTCAGATGGTCCTCACGTGCTGCTGGAGGAGCATGAAGGAA GTGGCTTTACTTCTTGGCACGCTCTGCCAGCTCCTCCCTCTGCAGCCTGTGCCAGAGTCTGCTGCTGGGT TACTGACAGTGCAGCAGGTTAAAGAAATAGGAGAATACTTTAAACAGCACCTTTTGCAGTCCAGACACAG AGGAGCATTTGAACTGGCTTATACAGGTTTTGTGAAACTCACTGAAATATTAAACAGGTGCTCTAATGTG AGTCTGCAAAAGCTGCCGGAGCAGTGGTTGTGGAGTGTTTTGGAGGAAATTAAAAGCAGTGATCCCTCAT CCAAACTCTGTGCTACAAGGCGTAGTGCTGGAATTCCTTTCTACATACAGGCATTGTTGGCATCTGAACC AAAGAAGGGTAGAATGGACTTGTTGAGAGTAACAATGAGGGAGCTGATCTCTTTGGCTTTGTCTGCAGAT GACTCAAAGGGCAGAGTTCCCCAGGTTCATGCTTTAAATATCCTCAGAGCCTTGTTCAGAGATACACGTC TGGGAGAAAACATTATTCCTTATGTTGCTGGTGGAGCTAAGGCTGCAATTCTGGGCTTTACATCACCTGT CTGGGCAGTGAGAAATTCATCCACACTTCTCTTTAGTTCCTTGATCACAAGAGTTTTTGGAGTTAAGAGG GGAAAGGATGAACTTTCCAAAACCAATAGAATGACAGGAAGAGAGTTTTTCTCTCGCTTCCCAGAACTGT ATCCCTTTCTTCTCAAACAATTGGAAACTGTAGCCAATACAGTGGACAGTGACCTGGGAGACCCTGATGG CCACCCCGGCATGTTCCTCTTACTCCTGGTGCTAGAGAGACTCTACCCATCTCCGATGGATGGCACGGCG TCTACACTCAGCTTGGCCCCTTTTGTTCCTTTCATTATCAGGTGTGGTCGCTCACCTTTTTACCGCTCCC GTGAAATGGCAGCTCGTGCATTGGTCCCCTTCATTACGATAGACCAGATACCAAGTACCCTCCATGCTCT GCTGGACTCACTCCCCAGCAGCACTGACCAGTGTTTCCGACAAAACCATATTCATGGGACACTTCTGCAA GTTTTTCATTTGTTACAAGCCTACATTACAGACTCCAAACACAGAATAAATGCAGATTTCCTTCAGGAGC TGAGTGACATCACTGTTTGTACCAAAGCCAAACTCTGGCTAGCCATGAGGCAAAATCCATGCTTGGTAAC CAGAGCTGTGTATATTGATATTCTCTTCCTGCTGACTCACTGCCTGGACAGACCCGAGGAAGGGAAGCAC ACAGCTCTGGAGAGTCTTGACTTCTGGGAAGATGTGAGAAGAATTATCTTGGGATCAGAGCTGATCACGG GGTTCCCCTGGACCTTCAAGGTTCCAGGCCTGCCCCAGTACCTGCAGAGCCTTACCAAGTTAGCCATCCC TGAAGTGTGGGCATCGCTGGCTGAAGCTGAAGGGCAGGCTACTGCTGTTCCTCTCTCCTTTTCTCAACTT CTCAAGTCTTCCTTCCCTGAAGTGCGCTTGCTGGCCCTGGATGCACTGCTGGAAAGGGCGGCTTCCTCTG AACAGGGGCAGAAGGGTCCCCCTCCCTTGCTGTACAGCATGGGAGAGGAGTTCCTACTGCTGGCAATGAA GGAAGACCACCCAGGATGTTTCTGCAGGGTCCTGAAGATTCTCTATCACTTGAACCCCAGTGAATGGCTT CCTCAGACAGAGTGCTCTGTTCACCTCTCCCCAAAGGAGTTCCTGGTCTGGACTATGGATATTGCTTCCA ATGACAGATCTGAAATCCAAGGTGTATCTCTGAGGCTTGCCTCCAAAATAATTGCCTACCTTGTGCAGAG TTGTGAGAAGAACAAAGATTCAGTGGCCCTGGAGCTGAGGCGGTGGGTCCAGCTGATTGTCTGGTCCTGT GGAGACCACCTTCCTACTGCATCTAGACTGGCTGTGGCTGAAGTCCTCTCAGGCACCGCACCCCTGTTCC TCACCAGTCCCCAGCCCATTCTAGACCTGCAGGATACACTTTCTCTCTGGAGGTGTGTCCTCACCCTTCT GCAGAGTGAGGAACAACCCGTCCGAGAGGCAGCCACAGAGACCGTGACAACAGCCATGTCACAAGGAAAC ACTTGCCAGTCAACAGAATTTGCCTTCTGCCAGGTGGATGCCTCCATTGCTCTCACCCTGGCCTTCGCTG TCCTGTGTGACTTGCTCCAGCAGTGGGACCAGCTGGGGCCAGGACTGCCCATTCTCCTGGGATGGCTGCT GGAAGAGGGTGGTGACCTCAAAGACCTCATGCAGAGCCCACATCAGGGGGAAGAAGAGCACATATTTGAA AAATCAGAAGTCAACTTCTGGGCCGAGACTCTGACCTTCGTGAAATACCTGTGTAATCAAGTCTTCCATC TCCTCTGTCAGTCTGGCTGTCAGTCCTCTCACTCCCAGCTCAGTCACTTGCAAAGGACAGCGTCAGAGCA GCATCGCCTCATCTCTCAGCTCTTCAGAGAACTTCCACTCTCGGCTGAATTTTTGAAGACAGTAGAATAC ACAAGACTGCGCATTCAAGAGGAAAGGACTTTGGCTGTCCTGCGGCTGTTGGCCTGTCTGGAAGGAAAGG AAGTCCTCAGAGCTGAAGACTGCCCTAGAGAATGGAGACAAGAAATGGCCCCAAGAACAGAAGCAGCTTG TTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191769
Insert Size	5814 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191769.1, NP_001178698.1
RefSeq Size	6870 bp
RefSeq ORF	5814 bp
Locus ID	313865

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