Arfgef1 (NM_001277056) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	BIG1; RGD1560793
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217698 representing NM_001277056 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATGAGGGCAAGAAGACGAAGAACATGTTCCTGACCCGGGCCCTGGAGAAGATTTTGGCCGACAAGG AAGTGAAGAAGGCGCATCACTCCCAGCTGCGCAAAGCGTGCGAGGTGGCCCTAGAGGAAATAAAAGTTGA AACTGAGAAACAAAGTCCTCCTCATGGAGAAGCAAAAGCTGGATCAGGCACCCTTCCACCAGTGAAATCA AAGACAAATTTTATTGAAGCAGACAAGTACTTCTTACCCTTTGAGTTGGCATGCCAGTCCAAATGTCCCC GCATAGTTAGTACATCTCTAGACTGTTTGCAGAAACTTATTGCTTATGGGCACTTGACTGGCAGTGCTCC AGATAGTACGACACCAGGCAAAAAGTTAATTGATAGAATTATTGAAACAATATGTGGCTGCTTCCAAGGC CCTCAAACAGATGAAGGAGTTCAGTTGCAGATAATAAAGGCTTTACTTACTGCAGTAACATCACAACACA TAGAAATTCACGAAGGAACTGTGCTGCAAGCTGTGAGGACATGCTACAATATCTATTTAGCCAGCAAAAA TCTCATCAATCAGACAACAGCCAAAGCTACTCTTACTCAGATGCTAAATGTTATCTTTGCACGCATGGAA AACCAAGCATTACAGGAAGCCAAACAAATGGAAAGAGAAAGACATCGGCAGCAGCATCACTTGTTACAGT CTCCAGTAAGCCATCACGAGCCCGAGTCACCTCATCTTAGATATTTGCCACCTCAGACTGTTGATCATAT AGCCCAAGAACAGGAAGGGGATCTTGATCCCCAAACACACGATGTGGATAAAAGTCTCCAAGATGACATA GAGCCTGAGAACGGATCTGATATTTCCAGTGCAGAGAATGAACAGACAGAAGCTGATCAGGCAACTGCAG CTGAAACATTATCTAAAGATGATGTGTTGTGTGATGGAGAGTGTGAGGAAAAGCCACAGGATATTGTGCA GAGCATTGTGGAAGAGATGGTGGACATCATTGTTGGAGACATGGGGGAAGGAACAGCTGTAAGTGCAAGT GCAGATGGCAACGCTGGAGCTGTAGAGGATGGCAGTGACAGTGAGAACGTGCAAGCCAATGGGATCCCGG GAACACCCATCTCTGCCGCATACACACCATCGTTACCTGACGACAGATTGTCTGTCTCCTCCAATGATAC TCAGGAATCTGGAAATTCTTCAGGACCTTCACCTGGTGCTAAGTTTTCCCACATTTTACAAAAGGATGCC TTTCTAGTGTTCAGGTCATTGTGTAAGCTGTCAATGAAACCACTGTCAGATGGACCACCAGATCCAAAGT CTCATGAGTTACGGTCCAAGATTCTTTCATTGCAGTTGCTTCTGTCCATTCTGCAGAATGCAGGACCTGT TTTCAGGACAAATGAGATGTTTATTAATGCTATCAAGCAGTATCTGTGTGTTGCACTCTCAAAAAACGGA GTCTCATCTGTTCCAGAGGTTTTTGAGCTTTCTCTTTCCATATTTCTAACTTTGTTGTCAAACTTCAAGA CACATCTGAAGATGCAAATTGAGGTATTCTTTAAAGAAATTTTCCTGTACATTTTGGAAACTTCTACAAG CTCATTTGATCATAAGTGGATGGTGATTCAGACACTGACAAGGATCTGTGCAGATGCTCAGAGTGTGGTG GATATTTATGTAAATTATGACTGTGACTTGAATGCAGCAAATATATTTGAAAGACTAGTAAATGACCTGT CAAAAATTGCCCAAGGAAGGGGCAGTCAAGAACTTGGGATGAGTAATGTTCAGGAATTGAGCCTGCGGAA AAAGGGGTTAGAATGTTTAGTTTCAATTCTTAAGTGTATGGTTGAATGGAGTAAGGATCAGTACGTGAAT CCCAACTCTCAAACCACCCTTGGTCAGGAAAAACCCTCAGAGCAAGAGATAAGTGAAATCAAACACCCCG AGACTATAAACAGATATGGAAGTTTAAACTCCTTAGAATCCACATCATCTTCAGGGATTGGCAGCTACAG CACACAGATGTCTGGCACTGATAATCCAGAACAATTCGAGGTCCTAAAGCAGCAAAAAGAAATAATAGAA CAAGGGATTGATTTATTTAATAAGAAACCAAAGAGAGGAATACAGTACCTCCAAGAACAAGGGATGCTTG GCACCACTCCTGAAGATATAGCTCAGTTCTTACATCAAGAAGAAAGATTAGACTCTACACAGGCGGGGGA GTTCCTGGGAGATAACGATAAGTTCAATAAGGAAGTCATGTATGCATATGTAGACCAGCATGACTTTTCG GGAAAAGACTTTGTTTCAGCTCTTCGTCTGTTTCTGGAAGGATTCCGGCTTCCAGGAGAAGCTCAGAAAA TTGATCGATTAATGGAAAAATTTGCTGCAAGATACCTAGAGTGCAACCAAGGACAAACCCTTTTTGCTAG TGCAGATACTGCTTATGTTTTGGCCTACTCAATTATCATGTTGACCACAGACCTTCACAGTCCACAGGTT AAAAACAAAATGACGAAAGAACAGTATATTAAAATGAATCGTGGTATCAATGACAGTAAAGATCTCCCTG AAGAGTATCTATCTGCCATTTATAATGAGATAGCTGGGAAAAAAATATCAATGAAAGAAACAAAAGAACT AACAATCCCTACAAAATCAACTAAACAGAATGTAGCCAGTGAAAAGCAAAGACGACTTCTGTATAATTTA GAAATGGAACAGATGGCCAAGACAGCTAAAGCACTCATGGAAGCTGTGAGCCATGTTCAGGCACCTTTTA CAAGTGCAACACACTTGGAGCACGTGAGGCCTATGTTTAAGTTGGCTTGGACCCCTTTTCTGGCTGCCTT CAGTGTGGGTCTACAAGACTGTGATGATACTGACGTGGCCTCTCTCTGCCTCGAAGGGATACGATGTGCG ATCAGAATTGCATGCATCTTCAGCATTCAGCTGGAAAGAGATGCATATGTCCAGGCACTAGCCAGATTTA CCTTACTTACAGTGAGTTCTGGTATTACTGAAATGAAACAGAAGAATATCGACACAATAAAAACACTCAT CACAGTGGCTCACACAGATGGAAATTATTTAGGAAATTCGTGGCATGAGATCCTGAAGTGCATTAGTCAG TTAGAGCTTGCACAACTTATAGGAACTGGAGTGAAGCCTCGGTACATTTCTGGGACCGTGAGAGGCAGAG AAGGGTCTCTCACTGGGACAAAAGATCAGGCTCCGGATGAATTTGTTGGTCTGGGGCTAGTTGGAGGAAA TGTGGACTGGAAGCAGATAGCCAGTATTCAGGAGTCTATTGGAGAAACCAGTTCTCAAAGTGTCGTGGTT GCTGTAGACAGAATATTCACAGGATCCACAAGGCTAGATGGAAATGCTATTGTCGATTTTGTTCGTTGGC TCTGTGCCGTGTCTATGGATGAATTGCTTTCCACTACACACCCAAGAATGTTTAGTCTACAAAAAATAGT AGAAATATCATACTATAACATGGGAAGAATAAGACTGCAGTGGTCTCGGATTTGGGAAGTTATTGGAGAT CATTTTAATAAGGTTGGTTGCAATCCTAATGAAGATGTAGCCATATTTGCAGTAGACTCCCTGAGGCAGC TATCGATGAAGTTTTTAGAGAAAGGAGAGCTTGCAAACTTCAGATTCCAAAAGGATTTCTTAAGACCTTT TGAACATATAATGAAACGAAACAGGTCTCCAACAATTCGAGATATGGTCGTGCGTTGTATAGCGCAGATG GTTAACTCTCAGGCTGCCAATATCCGTTCAGGATGGAAGAACATTTTCTCGGTGTTCCACCTAGCTGCAT CAGACCAGGATGAGAGCATAGTAGAGCTAGCATTCCAGACGAGCGGGCACATCGTCACTCTTGTATTTGA AAAGCACTTTCCAGCCACTATTGATTCTTTCCAGGATGCAGTGAAGTGTTTATCTGAATTTGCGTGTAAT GCGGCTTTCCCAGACACAAGTATGGAAGCAATCCGACTTATTCGCCATTGTGCAAAATATGTGTCTGATA GACCTCAGGCTTTCAAAGAGTACACAAGTGATGACATGAACGTGGCACCTGAAGACAGGGTGTGGGTGAG AGGGTGGTTCCCAATCCTCTTTGAGTTGTCCTGTGTCATCAATAGATGCAAATTAGATGTAAGAACCAGG GGCTTAACAGTGATGTTTGAAATAATGAAAACATACGGCCACACTTACGAAAAACACTGGTGGCAGGACT TGTTCAGAATTGTTTTCAGAATCTTTGACAACATGAAGTTGCCAGAACAGCAGACAGAGAAAGCTGAATG GATGACAACAACTTGTAACCATGCCCTTTATGCGATATGCGACGTGTTCACCCAGTATTTAGAAGTCCTC AGTGATGTACTTTTGGATGATATTTTTGCCCAGCTTTACTGGTGTGTACAACAAGACAATGAGCAGTTAG CACGATCTGGTACGAACTGCTTAGAAAATGTGGTTATTCTGAATGGTGAGAAGTTTACTCTAGAAATCTG GGATAAAACCTGTAATTGCACCCTGGATATCTTCAAAACCACAATTCCTCATGCGCTTCTGACTTGGCGT CCTACTTCTGGAGAGGCTGCCCCCCCATCTCCATCAGCTATGAGTGAAAAGCAGTTGGATGCAATATCAC AGAAATCTGTAGACATACATGATTCTGCTCAACCAAGATCTTCAGATAACAGGCAGCAAGCACCACTTGT TTCTGTTTCTCCTGCGAGTGAAGAAGTTAGCAAAGGGAGACCCACAGCAAAATTCCCAGAACAAAAATTG TTTGCTGCCCTGTTGATTAAATGTGTTGTGCAGCTGGAACTCATCCAGACCATCGACAACATTGTGTTCT TCCCAGCCACAAGCAAGAAGGAAGATGCAGAAAACTTAGCTGCAGCCCAGAGAGATGCAGTGGACTTCGA TGTTCGAGTTGATACTCAAGACCAAGGAATGTACCGCTTTTTAACATCACAACAACTCTTCAAGCTACTG GACTGCTTATTAGAGTCACATAGATTTGCAAAAGCATTTAATTCCAACAATGAACAGAGGACTGCTCTAT GGAAAGCAGGCTTCAAAGGCAAGTCCAAGCCCAATCTCCTAAAACAAGAGACAAGCAGCCTGGCGTGTGG ACTGCGCATCCTCTTCCGGATGTACACAGACGAGAGCAGAGTCAGCGCCTGGGAGGAGGTCCAGCAGAGG CTTTTAAACGTCTGCAGTGAGGCACTGAGTTACTTCCTCACTCTAACATCTGAAAGTCACCGGGAAGCCT GGACCAACTTACTGTTACTGTTCCTCACTAAAGTCCTCAAGATCAGTGACAACAGGTTTAAAGCTCACGC ATCATTCTACTACCCTCTGTTATGTGAAATTATGCAATTTGACTTGATTCCTGAACTTCGTGCTGTTCTT AGAAGATTTTTCCTGCGAATTGGAATAGTATTTCAGATATCACAACCACCTGAACAGGAACTTGGGATAA ACAAGCAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001277056
Insert Size	5541 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001277056.1, NP_001263985.1
RefSeq Size	6945 bp
RefSeq ORF	5541 bp
Locus ID	312915
UniProt ID	D4A631
Gene Summary	Promotes guanine-nucleotide exchange on ARF1 and ARF3. Promotes the activation of ARF1/ARF3 through replacement of GDP with GTP. Involved in vesicular trafficking. Required for the maintenance of Golgi structure; the function may be independent of its GEF activity. Required for the maturaion of integrin beta-1 in the Golgi. Involved in the establishment and persistence of cell polarity during directed cell movement in wound healing. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways. Inhibits GAP activity of MYO9B probably through competetive RhoA binding. The function in the nucleus remains to be determined (By similarity).[UniProtKB/Swiss-Prot Function]

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