Psme4 (NM_001025140) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217697 representing NM_001025140 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCTCCGGAGCGGGCGGGTGGCGGGGAGCCCCCCGAGCCTGGCGGGCGGCCGGTACTGGGCCCGC GGGCCTTCGTCCCGCAGAAAGAGATCGTGTACAACAAGCTTCTGCCCTACGCCGAGCGGCTGGACGCCGA GTCCGACTTGCAGCTGGCGCAGATCAAGAGCAACCTGGGCCGGGCAGTGCAGCTCCAGGAGCTGTGGCCG GGCGGCCTCTTCTGGACGCGGAAACTCTCCACTTATATTCGACTTTATGGGAGAAAATTTAGCAAAGAAG ATCATGTTCTTTTTATTAAGTTATTGTATGAGCTGGTATCAATTCCAAAACTAGAAATCAGCATGATGCA GGGATTTGCCCGTCTCTTGATCAACTTATTAAAAAAAAAGGAACTTCTTTCAAGAGATGATTTAGAGTTA CCATGGAGACCACTTTATGACATGGTAGAAAGAATATTATATTCCAAGACAGAGCACCTGAGATTAAATT CATTTCCTAATTCTATAGAAAATGTTCTCAAAACCCTCGTGAAAAGCTGCCGACCATATTTTCCAGCAGA TGCAACAGCAGAAATGTTAGAAGAATGGAGGCCTTTAATGTGTCCTTTTGATGTAACAATGCAAAAGGCC GTCAGTTATTTTGAAATATTTCTTCCTACCTCCCTTCCTCCAGAACTGCATCATAAAGGTTTTAAACTTT GGTTTGATGAATTAATTGGCCTTTGGGTTTCAGTGCAAAATCTCCCACAGTGGGAGGGGCAATTAGTAAA TCTCTTTGCTCGATTGGCTACAGATAATATAGGATACATAGATTGGGATCCATATGTACCAAAGATATTT ACAAGAATTCTGAGAAGCCTGAACCTCCCAGTGGGAAGTAGTCAAGTGTTAGTCCCAAGATTTTTAACAA ATGCTTATGATATAGGACATGCTGTAATATGGATCACCGCAATGATGGGTGGACCAAGTAAATTAGTACA AAAACATTTGGCTGGTCTGTTTAACAGCATCACATCTTTTTATCATCCTTCAAATAATGGGCGTTGGCTG AACAAGTTAATGAAACTCCTTCAGCGGTTGCCAAATAGTGTTGTTAGGAGATTACATCGTGAAAGATTCA AGAAGCCCTCCTGGTTAACTCCTGTGCCTGAAAGCCATAAGCTTACGGATGAAGATGTTACAGACTTTGT ACAATGCATTATTCAGCCTGTCCTCTTGGCTATGTTCAGCAAAACTGGTAGTCTAGAAGCAGCCCAGGCG CTGCAGAACCTTGCACTCATGAGACCGGAGTTAGTAATACCTCCTGTGCTTGAAAGAACATACCCTGCTT TAGAGACATTAACAGAACCTCACCAGCTCACAGCTACTTTAAATTGTGTAATTGGAGTAGCCCGAAGTTT GGTATCAGGCAGCAAATGGTTTCCTGAAGGTCCAACACACATGCTACCTCTGTTGATGAGAGCATTGCCT GGGGTAGATCCAAACGACTTCAGTAAATGCATGATCACATTCCAATTTATAGGAACATTTTCTACTCTGG TGCCTTTAGTAGACTGTTCATCTGTACTGCAAGAAAGAAATGACCTCACAGAAATTGAAAAAGAACTTTG TTCAGCAACAGCTGAATTTGAGGATTTTGTGTTACAATTTATGGACAGATGCTTTGGACTAATAGAAAGT AGCACATTGGAGCAAACAAGGGAAGAGACAGAAACAGAGAAAATGACTCATTTGGAGAGTTTGGTTGAAT TAGGCTTGTCTTCTACGTTTAGTACAATCCTCACACAATGTTCCAAAGACATATTTATGGTAGCCCTTCA GAAGGTCTTTAACTTTTCTGTCTCACATATATTTGAAACAAGAGTTGCAGGTCGCATGGTGGCAGACATG TGCCGAGCTGCTGTAAAGTGTTGCCCGGAAGAATCCCTGAAGCTCTTTGTTCCCCACTGCTATGGTGTTA TAACTCAGCTTACAATGAATGATGATGTACTGAATGAAGAAGAGCTAGACAAGGAGTTGCTATGGAACCT TCAGCTTCTGTCTGAGATTACTCGTGTGGATGGGAAGAAGTTACTTCTATATAGGGAGCAGCTTGTAAAG ATTCTCCAACGAACCTTACACCTGACCTGTAAGCAAGGCTACACACTGTCTTGCAACCTTTTGCATCATC TTCTTCGTTCTACGACACTAATCTACCCCACAGAGTACTGCAGTGTTCCAGGTGGCTTTAACAAGCCGCC TTCTGAATACTTCCCTATCAAGGACTGGGGAAAGCCTGGAGATCTGTGGAATCTGGGGATACAGTGGCAT GTTCCTTCTTCAGAGGAGGTCTCTTTTGCCTTCTATTTGTTACAATCCTTTCTTCAGCCTGAGCTTATCA AACTCCAGCGTTGTGGAGATGGAGAACTTGAAATGTCTAGAGATGATATTCTACAGAGTCTGACTATAGT ACACAACTGCTTGATTGGTTCAGGGAACCTTCTACCTCCACTGAAAGGAGAGCCAGTTACTAACTTGGTA CCAAGTATGGTGTCCTTAGAGGAAACAAAATTGTATACCGGACTGGAACATGACCTATCCAAAGAAAACC ACCGAGAAGTAATTGCTAGTGTCATAAGGAAACTTCTTAGCCACATACTTGATAATTCAGAAGATGATAC CAAATCGTTGTTTCTTATCATAAAGATCATTGGAGACCTTCTGCAATTCCAAGGATCTCATAAACATGAA TTTGACTCTCGCTGGAAAAGCTTTAATTTAGTAAAGAAATCAATGGAAAATCGGCTCCATGGAAAAAAGC AACATATCAGAGCACTACTAATTGACAGAGTGATGCTGCAGCACGAGTTGCGAACACTAACTGTTGAGGG TTGTGAGTACAAAAAAATACATCAAGATATGATCAGAGATCTTCTTCGATTATCAACAAGTTCATACAGT CAGGTCAGAAACAAAGCTCAGCAAACATTTTTTGCTGCTTTAGGAGCCTATAACTTCTGTTGCAGAGATA TCATCCCCTTGGTTTTGGAGTTCTTACGGCCTGATAGAAAGGATGTCACACAGCAGCAGTTCAAGGGCGC CCTATATTGTCTACTTGGAAATCACAGTGGCGTGTGCCTGGCAAACCTTCATGACTGGGACTGTATTGTG CAGACTTGGCCAGCACTTGTTTCTTCAGGACTTAGCCAAGCAATGTCACTGGAAAAGCCATCAATAGTGA GACTATTTGATGACCTTGCTGAAAAGATTCATAGACAATATGAAACAATTGGCTTAGACTTCACAATTCC AAAGTCATGTGCTGCAATAGCAGAATTACTTCAACAATCAAAGAATCCTTCCATTAGCCAGACACTACTT AGCCCAGAAAAGATTAAAGAAGGACAGAAGCGTCAACAGGATAAGAATGCTGATGCTGTAAGGAACTATG AATGTTTGGTAAATACTTTACTAGATGGTGTGGAGCAAAGAAATCTGCCTTGGAAATTTGAGCATATAGG AATCGGGCTTCTGTCTTTACTCCTAAGGGATGACAGAGTGTTGCCTCTTCGTGCCATACGTTTTTTTGTT GAAAATCTCAACCATGATGCAATCGTTGTTCGAAAGATGGCTATCTCAGCTGTTGCTGGTATTCTCAAGC AGCTAAAGAGAACTCACAAAAAGCTGACCATCAACCCTTATGAAATCAGTGGATGTCCTAAACCTGCCAC AGTTCTTGCTGGTGATAGACCTGATAACCATTGGTTGCATTATGATAGCAAAAATATACCAAGAACAAAA AAAGAATGGGAGTCAAGTTGCTTTGTTGAAAAAACTCACTGGGGATACTACAACTGGCCAAAGAACATGG TTGTTTATGCTGGTGTGGAAGAGCAGCCTAAACTTGGCAGAAGCAGGGAGGACATGACAGAGGCAGAACA GATTATATATGATCGTTTTTCTGACCCTAAGTTTGTTGAGCAGTTAATCACATTTCTATCTTTAGAAGAC AGAAAAGGAAGAGATAAGTTTAGTCCCCGGCGCTTCTGCCTCTTTAAGGGTATATTTAGAAATTTTGACG ATGCTTTTTTGCCTGTTCTGAAGCCCCATTTAGAACGTTTGGTTGCAGATTCTCATGAAAGTACCCAGAG ATGTGTTGCAGAAATTATAGCTGGTTTAATCAGAGGTTCTAAGCATTGGACGTTTGAGAAGGTGGAGAAG CTTTGGGAGCTCCTATGTCCGCTGCTGAGAACAGCACTGTCCAACATGACAGTGGAGACGTACAATGACT GGGGAACCTGTATAGCCACATCCTGTGAAAGCAGAGATCCCCGGAAGCTTCATTGGCTTTTTGAGCTGCT CTTGGAGTCACCATTGAGTGGTGAAGGGGGGTCCTTTGTAGATGCATGCCGACTCTATGTGCTACAAGGT GGCCTTGCCCAGCAAGAATGGAGAGTCCCTGAACTATTGCACAGACTTCTGAAGTACCTGGAACCGAAAC TCACCCAGGTTTACAAAAATGTCAGAGAAAGGATAGGGAGTGTTCTGACCTACATATTCATGATCGATGT TTCTTTGCCAAATACTGCTCCAACAACATCTCCTTGTATCCCTGAGTTTACTGCGCGAGTCCTAGAAAAA CTGAAACCTCTCACAGATGTGGATGAAGAAATTCAGAACCATGTCATGGAAGAAAATGGCATTGGTGAAG AAGATGAACGGACTCAGGGCATTAAACTCTTAAAAACCATATTGAAATGGCTTATGGCAAGTGCAGGAAG GTCATTTTCTACAGCAGTCAAAGAGCAGCTTCAGCTTCTGCCTCTGTTCTTTAAGATCGCCCCAGTGGAA AATGACAATAGTTATGATGAGTTGAAAAGAGATGCAAAGCTCTGTTTATCACTAATGTCTCAGGGGTTGC TCTACCCTCAGCAAGTGCCTTTGATACTTCAGGTGCTAAGCCAGACAGCAAGAAGCAGTTCTTGGCATGC AAGATATACAGTGCTGACCTACCTCCAGACCATGGTATTTTATAACCTCTTTATTTTCCTAAACAATGAA GATGCAGTTAAAGACATCAGATGGCTGATTATACGCCTTTTGGAGGATGAACAGTTAGAGGTTCGAGAAA TGGCTGCTACTACCTTAAGTGGTTTGTTACAGTGTAACTTCCTTACCATGGACAGTGCTATGCAGAGTCA TTTTGAGCAACTTTGCAAAACTAAACTACCCAAGAAAAGAAAACGAGACCCTGGTTCTGTGGGAGATACC ATTCCTTCTGCAGAGTTGGTCAAACGTCATGCTGGTGTGCTTGGACTTGGTGCATGTGTTCTTTCTAGCC CTTATGATGTTCCTACCTGGATGCCCCAGCTCCTTATGAATCTCAGTGCACATTTGAATGATCCTCAGCC TATTGAAATGACTGTCAAGAAGACCTTATCCAATTTCCGAAGGACACATCATGACAACTGGCAAGAACAC AAGCAGCAGTTCACTGATGACCAGCTGCTTGTCCTCACTGACCTCCTTGTGTCACCATGCTATTATGCAT AG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001025140
Insert Size	5532 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001025140.2, NP_001020311.2
RefSeq Size	7327 bp
RefSeq ORF	5532 bp
Locus ID	498433

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