Ltbp4 (NM_001170336) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217673 representing NM_001170336 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCCGGCCTGGCCTCGGCTGCCACTGCCCCCTCCTCCTCCTGCTGCTGCTCCCAGCCGCCACATCTG CTAGCGGCCCCAGTTCCAGTCCCAGCCCTATCCAGGCCGTCGAGGACGCGGTGGTCCCGGGCCACCAGGC CGGTGCTGCTGCCTGTCGTTGCTGCTTAGCCCAGACACCTAGGAGTAGCCACTGCACCCGAGCTTCCTGC AGGGTCCGGAGCTGCCCACCCGGCAGGTGTACAGATCAACAGGGGTGCCTGACCCCAACACCTTCGGTGC CCAGCCCCAGCCGCAGTCCGGTGGAGAAGAGACAGGTATCCCTCAACTGGCAGCCACTGACTTTGCAGGA GGCCCGAGCTCTGCTGAGGCAAAGGCGGCCCCGAGGGCCGTGGGCCCGGGCATTGCTGAAAAGAAGGCCC CCACATCGCGCCCCTGCCGGCCAGGCCCGGGTTCTATGTCCCTTGATCTGTCACAACGGTGGTGTGTGTG TGAAGCCTGACCGCTGCCTTTGCCCCCCGGACTTCGCTGGCAAATTCTGTCAATTGCATTCTTCGGGGGC CCGGCCCCCGGCCCCAGCCATGCCGGGCCTTACCCGTTCGGTTTACACAATGCCACTAGCCAATCACCGC GATGATGAACACGGCGTAGCATCGATGGTGAGTGTGCACGTGGAGCACCCTCAGGAGGCATCGGTGGTGG TGCACCAGGTGGAACGTGTGTCTGGCCCCTGGGAGGAGGCGAACTCTGAAGCCCTAGCCAGGGCTGAAGC GGCAGCACGGGCCGAGGCGGCGGCGCCCTACACAGTGCTGGCACAGAGCGCGCCACGCGAGGACGGCTAC TCAGACGCCTCCGGCTTTGGTTACTGTTTCAGGGAACTGCGCGGAAGCGAATGTGCATCGCCGCTGCCGG GACTCCGGACGCAGGAAGTATGCTGCCGAGGGGAGGGCTTGGCCTGGGGTGTTCATGATTGTCACCCGTG CGCGGAACACTTGGGGAACTCCAACCGAGTGAGTGGTCCAAATGGGCTTTGTCCAACCGGATTCGAAAGA GTTAATGGGTCCTGTGTAGATGTGGATGAGTGCGCCACAGGTGGGCGTTGCCAACACGGAGAGTGTGCCA ACACTCGTGGCGGGTACACGTGTGTATGTCCGGATGGTTTCCTCCTAGACTCTTCCCGCAGCAGCTGCAT CTCCCAACACGTGATCTCGGAGGCAAAGGGGCCCTGCTACAGAGTGCTCCAAGATGGCGGATGCTCGCTG CCCATTCTTCGAAATATCACCAAACAGATCTGCTGCTGTAGCCGCGTGGGCAAGGCGTGGGGCCGAGGCT GCCAGCTCTGTCCACCTTATGGTTCAGAGGGTTTTCGGGAGATCTGTCCAGCTGGCCCTGGTTACCACTA TTCAGCTTCTGACCTCCGCTACAACACCAGACCCCTAAACCCGGATCCACCTCGAGTGACTTTCAATCAG CCACGTGCTCCACCTGTCACTCTTAGGCCACCCACAGGTTTTCTGCCCACTCGTCGACCTGAGCCCCGTC CTGACCCTGGGCCACAGCCTGAACTCCGGCCTCGACCTGAACCCCAGCCTCGGCCTGAATTTCCCCTGCC CAGCATCCCTGCTTGGACCGGTCCAGAGATTCCTGAATCAGGTACCTCTTCCAGCATGTGTCAGCGAAAT CCCCAGGTTTGTGGTCCTGGACGCTGCGTTTCTCGGCCAGGCGGCTACACCTGTGCCTGCGACCCAGGTT TCCGGCTCGGCCCCCAGGGCACTCGCTGTATTGACGTAGATGAATGTCGCCGCGTCCCTACACCCTGTGC TCCTGGACGCTGCGAGAATACACCAGGCAGCTTTCGCTGCGTGTGCGGCACCGGCTTCCGAGCAGGCCCA CGGGCTACAGAGTGTTTGGATGTGGACGAGTGCCGCCGAGTGCCGCCGCCGTGTGACCGCGGGCGCTGCG AGAACACACCAGGAAGTTTCCTATGTGTGTGTCCCGCCGGGTACCAGGCAGCACCGCACGGAGCCAGCTG CCAGGATGTTGATGAATGCACCCAGAGCCCAGGCCTCTGTGGCCGCGGGGCCTGCGAAAACCTGCCCGGC TCTTTCCGCTGTGTTTGCCCGGCTGGTTTCCTCGGCTCAGCGTGTGAAGAAGATGTGGACGAATGTGCCC AGCAGCCTCCGCCCTGCGGGCCAGGCCGCTGCGACAACACCTTGGGTTCATTCCACTGTGCCTGTCCAGC TGGCTTCCGCTCCCGAGGGCCAGGGGCCCCCTGCCAAGATGTGGACGAATGTGCCCGGAGCCCCTCACCT TGTGCCTATGGCCGCTGTGAAAACACGGAAGGGAGCTTCAAGTGTGTCTGCCCTACAGGCTTCCAACCCA ACGCTGCGGGCTCCGAGTGCGAGGATGTGGATGAGTGTGAGAACCACTTGGCCTGTCCTGGGCAGGAGTG TGTGAACTCACCAGGCTCCTTCCAGTGCCGGGCCTGCCCTGTTGGCTACCACCTTCACCGTGGCAGATGC ACTGACGTGGACGAATGCAATTCCGGCACCCCTTGTGGTCTCCATGGCCAATGCACGAATGCCCAAGGCT CCTTCCACTGCAGCTGCTCGACAGGTTACCGGGCACCATCTGGTCGACCCGGGCCCTGTGCAGATATAAA TGAGTGTCTGGAAGGCGACTTCTGCTTCCCCCATGGAGAGTGCCTCAACACCGACGGCTCCTTTACCTGT ACCTGTGCCCCTGGCTATCGGCCTGGACCTCGTGGAGCATCTTGTCTGGACGTGGACGAGTGCAGTGAGG AGGATCTTTGCCAGAGCGGCATCTGTACCAACACTGACGGCTCCTTCGAATGCATCTGTCCTCCTGGACA CCGTGCTGGCCCTGACCTTGCCTCCTGCCTTGACATTGACGAGTGTCGTGAACGGGGACCTGCCCTGTGC GGGTCTCAGCGCTGTGAAAATTCTCCTGGCTCCTACCGCTGTGTGCGGGACTGCGATCCAGGTTACCACC CTGGCCCCGAGGGCACCTGTGATGACGTGGACGAGTGCCGAGAATATGGCTCTGCGATTTGCGGCGCCCA GCGCTGTGAGAACACCCCTGGCTCCTACCGATGCACGCCAGCCTGCGACCCTGGCTATCAACCTACTCCA GGGGGCGGGTGCCAGGATGTGGATGAGTGCCGGAACCGGTCTTTCTGCGGTGCCCATGCTATGTGCCAGA ATCTGCCTGGCTCCTTCCAGTGCGTCTGTGACCAAGGCTACGAGGGGGCACGGGACGGGCGTCACTGCGT GGATGTGAATGAGTGTGAGACACTACAGGGTGTCTGTGGCTCCGCACTGTGTGAAAATGTTGAGGGCTCC TTCCTGTGTGTCTGCCCCACCAGCCCTGAGGAGTTCGATCCCATGACTGGACGTTGTGTTCCCCCTCGGA CTCCTGCAGGCACGTTCCCAGGCTCGCAGCCCCAAGCACCTGCCAGCCCCAGTCTTCCAGCCAGGCCTCC CGCCCCTCCCCCACCTCGAAGACCCAGTCCTCCCAGGCAGGGTCCCGTGAGCAGTGGGCGTCGTGAGTGC TACTTTGACACGGCAGCTCCAGATGCGTGTGACAATATCTTGGCTCGTAACGTGACGTGGCAGGAGTGCT GCTGTACTGTGGGAGAAGGCTGGGGCAGCGGGTGCCGCATCCAGCAGTGCCCGGGCACGGAGACAGCTGA ATACCAGTCATTGTGTCCTCACGGCCGGGGCTACCTAGTGCCCAGTGGAGACCTGAGTGCCCGGAGAGAT GTGGATGAATGCCAACTCTTCCAGGACCAGGTGTGCAAGAGCGGAGTGTGTGTGAACACTGCCCCAGGCT ACTCTTGCTATTGCAACAACGGCTTCTACTATCATGCGCACAGGCTGGAATGCGTTGATAATGACGAATG TGCCGACGAGGAGCCCGCTTGTGAAGGTGGCCGCTGCGTGAACACGGTGGGCTCTTACCACTGTACCTGC GAGCCCCCACTGGTGCTGGACGGCTCCAGGCGACGCTGCGTCTCCAATGAGAGCCAGAGCCTTGATGACA ATCTGGGAGTGTGCTGGCAGGAAGTCGGGCCTGATCTCGTCTGCAGTCGCCCACGACTAGACCGGCAGGC CACCTACACAGAGTGCTGCTGTCTCTATGGTGAAGCCTGGGGTATGGACTGTGCTCTCTGTCCTGCCCAG GACTCAGATGATTTTGAGGCTCTTTGCAATGTGCTGCGCCCACCTGCTTATGGCCCGCCACGCCCGGGTG GCTTTGGACTTCCTTATGAATATGGCCCAGATATAGGCCCACCTTATCAGAGTCTCCCCTATGGGCCAGA TCTGTATCCACCACCTGTGCTACCCTATGACCCCTACCCACCCCCACCTGGACCTTTTGCTCGCCGGGAG GCCCCTTATGGTGCCCCGCCCTTCGAAATGCCGGACTTTGAGGATGATGGTGACCCCTATGGCGAGACTG AGGCTCCTGACCCACCAAGCCGAGGCACTGGTTGGCCATATCGGTCCAGAGACACCCGTGGCTCCTTCCC AGAGCCTGAGGAATCCTCTGAGCGTGGAAGCTATACAGGAGCCTTGTCTGAGCCCTATGAGGGCCTAGAG GCCGAGGAGTGTGGTATCCTGGATGGCTGCGCCCACGGCCGCTGTGTACGTGTTCCAGAAGGCTTCACCT GTGATTGTTTCGACGGCTACCGCCTGGACATCACCCGCATGTCCTGCGTCGACATCAACGAGTGTGACGA GGCTGAGGCAACCTCCCCACTCTGCGTCAATGCGCGCTGTGTCAACACTGATGGTTCCTTCCGCTGTATT TGCCGTCCGGGATTTGCACCCACGCACCAACCACATCACTGTGCGCCTGCTCGACCTCGGGCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001170336
Insert Size	4896 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001170336.1, NP_001163807.1
RefSeq Size	5157 bp
RefSeq ORF	4896 bp
Locus ID	292734

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