Ptprz1 (NM_001170685) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Ptpz; PTPzeta-A; PTPzeta-B; PTPzeta-S; RPTPbeta
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217669 representing NM_001170685 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGAATCCTGCAGAGCTTCCTCGCGTGCGTTCAGCTACTGTGCGTGTGTCGCCTGGACTGGGCTTATG GATACTACAGACAACAGAGAAAACTTGTTGAAGAGATTGGCTGGTCCTATACAGGAGCACTAAATCAAAA AAATTGGGGAAAGAAATATCCAATATGTAATAGCCCAAAGCAGTCTCCTATTAATATTGATGAAGATCTT ACACAAGTAAATGTGAATCTTAAGAAACTGAAATTTCAGGGTTGGGAAAAACCATCCTTGGAAAACACGT TTATTCACAACACTGGGAAAACAGTGGAAATAAATCTCACCAATGACTACTATCTCAGTGGAGGACTTTC AGAAAAGGTCTTCAAGGCAAGCAAAATGACTTTTCACTGGGGGAAATGCAATGTGTCATCGGAAGGATCG GAACACAGCTTAGAAGGGCAGAAGTTTCCACTCGAGATGCAAATCTACTGCTTCGATGCGGACCGATTTT CAAGTTTTGAGGAAACAGTTAAAGGAAAAGGAAGGTTAAGGGCTCTATCCATTTTATTTGAGATTGGAGT TGAAGAAAATTTGGATTACAAAGCCATTATTGATGGGACTGAAAGTGTTAGTCGTTTTGGAAAGCAGGCT GCCTTAGATCCGTTCATCTTGCAGAACCTCCTGCCAAACTCCACTGACAAGTACTACATTTACAACGGAT CACTGACATCCCCTCCCTGCACAGACACCGTCGAATGGATTGTTTTTAAGGATACAGTTAGCATCTCTGA AAGCCAGCTGGCTGTATTTTGTGAAGTTCTCACAATGCAACAGTCCGGGTATGTCATGTTGATGGATTAC TTACAAAACAATTTCCGAGAACAACAGTACAAGTTTTCCAGGCAGGTGTTTTCTTCGTACACTGGGAAGG AAGAGATTCATGAAGCAGTGTGCAGTTCAGAACCAGAAAATGTGCAAGCTGACCCTGAGAATTACACCAG CCTTCTGATCACATGGGAAAGGCCTCGGGTTGTTTATGACACAATGATTGAGAAGTTTGCGGTTCTGTAC CAGCCACTGGAGGGAAACGACCAAACCAAGCATGAGTTTTTAACAGATGGCTATCAGGACTTGGGTGCCA TTCTCAATAACTTAATACCTAACATGAGTTATGTGCTCCAAATAGTGGCCATATGCTCTAATGGCCTTTA TGGAAAGTACAGTGACCAATTGATAGTCGACATGCCTACTGAGGATGCTGAACTTGACCTCTTTCCTGAA TTAATTGGAACTGAAGAAATAATCAAGGAGGAAGACTATGGAAAAGGCAATGAAGAAGACACTGGCTTGA ATCCCGGTAGAGACAGTGCCACAAACCAAATAAGGAAAAAGGAACCCCAGGTTTCTACCACAACTCACTA TAATCACATGGGGACTAAATATAATGAAGCCAAGACTAACCGATCTCCAACGAAAGGATCTGAATTCTCT GGAAAGAGTGATGTTCTCAACACATCCCTGAATCCTACTTCCCAACAGGTTGCTGAATTCAATCCAGAAA GAGAAATGTCCTTGCCTTCTCAGATTGGAACTAACCTGCCACCACACAGTGTGGAAGGCACCTCAGCCTC CTTAAACAGTGGCTCTAAAACTCTCCTTGTCTTCCCACAGATGAACTTGTCTGGGACTGCAGAATCCTTA AATATGGTTTCCATAACAGAGTACAAAGAGGTGTCTGCTGACCTCAGTGAGGAAGAAAACTTACTGACTG ATTTCAAGCTCGATAGTGGAGCAGATGATTCGTCAGGCTCTAGCCCTGCGTCCTCCACTGTCCCCTTTTC CACAGATAATCTATCCCATGGATATACATCTTCTTCAGATACGCCCGAGGCGGTCACGTATGATGTCCTT AGGCCAGAATCTACGAGAAATGCTCTAGAGGATTCGGCTCCATCAGGTTCAGAAGAATCACTAAAGGATC CCTCTCTTGAAGGGAGTGTGTGGTTCCCTGGATCCACAGACCTAACAACACAGTCTGAGACTGGATCTGG GAGAGAGGGCTTTCTCCAAGTTAACTCCACGGACTTCCAAGTTGATGAATCGAGGGAGACAACTGAGACA TTTTCTCCAGATGCTACCGCGTCCCGGGGTCCTTCGGTCACAGATATGGAAATGCCACATTATTCTACCT TTGCCTACCCCCCGACTGAAGTAACACCACATGCTTTCACTCCATCCTCCAGACCACTTGATTTGGCTCC CACTAGCAACATCCTCCATTCGCAGACAACTCAACCAGTATACAATGGTGAGACACCTCTTCAACCTTCC TACAGTAGTGAAGTCTTTCCTCTAGTCACCCCTTTGTTGCTTGACAATCAGACCCTCAACACTACCCCTG CTGCTTCAAGTAGTGATTCGGCCTTGCATGCTACGCCTGTATTCCCCAGTGTTGGTGTGTCATTTGACTC CATCCTGTCTTCCTATGATGATGCACCTCTGCTCCCATTTTCCTCTGCTTCCTTCAGTAGTGACTTGTTT CACCATCTGCATACGGTTTCTCAAACCCTTCCGCAAGTTACTTCAGCTGCTGAGAGGGATGAGCTGTCTT TGCATGCTTCTCTGCTGGTGGCTGGGGGTGATTTGCTGTTAGAGCCCAGCCTTGTTCAGTATTCTGATGT GATGTCACATCAGGTCACTATTCATGCTGCTTCGGACACATTGGAATTTGGTAGTGAGTCTGCTGTCCTT TATAAAACGTCTATGGTTTCTCAAATCGAATCACCCAGCAGTGATGTCGTTATGCATGCATATTCGTCAG GGCCTGAAACTTCTTATGCCATTGAGGGCTCCCACCACGTGCTCACTGTTTCTTCCAGTTCTGCAATACC TGTGCATGATTCTATTGGTGTAGCTGATCAGGGGTCCTTACTTATCAATCCTAGCCATATATCACTGCCT GAGTCCTCATTTATTACTCCAACTGCATCATTACTGCAGCTTCCTCCTGCCCTCTCTGGTGATGGGGAGT GGTCTGGAGCCTCCTCTGATAGTGAATTGCTTTTACCTGACACAGATGGGCTGAGAACTCTTAACATGTC TTCACCTGTTTCTGTAGCTGATTTTACATACACGACATCTGTGTCTGGCGATGATATTAAGCCGCTTTCT AAAGGTGAAATGATGTATGGAAATGAGACTGAACTGAAAATGTCTTCTTTCAGTGACATGGCATACCCTT CTAAAAGCACAGTCGTGCCAAAGATGTCTGATATTGTAAATAAGTGGAGTGAATCTTTAAAAGAAACCTC TGTTTCCGTATCTAGCATAAACAGCGTGTTTACAGAGTCTCTGGTTTATCCCATAACTAAGGTTTTTGAT CAGGAGATTAGTCGAGTTCCAGAGATTATCTTCCCAGTTAAACCTACACACACAGCATCTCAAGCATCTG GTGACACTTGGCTTAAACCCGGGCTTAGCACAAACTCAGAGCCTGCGCTCTCTGACACTGCTTCTAGTGA AGTATTACACCCTTCAACACAGCCCTTGCTCTATGAGGCCGCATCTCCTTTTAATACGGAAGCATTGCTG CAACCTTCCTTTCCGGCTTCTGATGTTGACACCTTGCTTAAAACTGCCCTTCCCTCTGGGCCTCGTGATC CAGTGCTGACTGAAACCCCCATGGTTGAGCAAAGTAGCTCTTCCATGTCTCTTCCCCTGGCATCAGAGTC TGCTTCAAGCAAAAGCACGCTGCACTTTACATCTGTACCAGTTCTCAATATGTCACCTTCTGATGTGCAC CCCACTTCACTTCAACGCTTAACAGTTCCTCACTCGAGGGAGGAATATTTTGAACAAGGTTTGCTTAAGA GCAAAAGTCCCCAGCAAGTCCTGCCGTCCTTGCACAGCCATGACGAGTTTTTCCAAACTGCACATCTGGA CATTAGCCAGGCCTACCCTCCAAAAGGAAGGCATGCATTTGCTACTCCTATTTTATCAATCGATGAACCA CAAAATACACTTATAAGCAGGCTTGTGTATTCTGAGGACATTTTCATGCACCCTGAAATTTCTATTACTG ATAAGGCACTTACTGGTCTACCAACGACCGTTTCTGATGTACTTATAGCTACTGACCATTCTGTTCCATT AGGAAGTGGGCCCATTTCCATGACAACTGTTTCTCCCAACAGAGATGATTCTGTGACCACAACCAAGTTG CTTCTTCCTTCTAAAGCTACTTCTAAGCCGACTCATAGTGCCAGATCTGATGCCGATTTAGTAGGAGGTG GTGAAGATGGTGATGACTATGATGATGATGATTATGATGACATAGATAGTGATCGCTTTCCCGTAAATAA GTGTATGTCATGTTCACCCTATAGAGAATCACAGGAAAAGGTAATGAATGACTCGGACACCCAAGAAAGC AGTCTTGTGGATCAGAGTGACCCAATTTCACATTTGCTCTCTGAGAATACCGAAGAAGAAAATGGAGGCA CGGGTGTAACTAGGGTGGATAAAAGTCCTGATAAGTCACCACCACCAAGTATGCTACCCCAGAAGCACAA TGATGGAAGAGAGGACCGTGACATTCAGATGGGTAGTGCTGTCCTTCCTCACACCCCAGGATCTAAAGCA TGGGCAGTTTTGACAAGTGATGAAGAGAGTGGGTCAGGGCAAGGCACCTCAGATAGCCTTAATGATAATG AGACTTCCACAGATTTCAGTTTCCCAGATGTTAATGAAAAGGATGCTGATGGTGTCCTGGAAGCAGATGA CACAGGCATAGCTCCGGGATCTCCACGGTCCTCCACACCATCTGTTACTAGTGGGCATTCAGGAGTATCC AACAGTTCAGAGGCAGGTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001170685
Insert Size	4851 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001170685.1, NP_001164156.1
RefSeq Size	6844 bp
RefSeq ORF	4851 bp
Locus ID	25613
UniProt ID	Q62656
Gene Summary	alternative splicing product phosphocan inhibits neural cell adhesion and neurite outgrowth [RGD, Feb 2006] Transcript Variant: This variant (2), known as phosphacan, differs in the 3' UTR and 3' coding region, compared to variant 1. The resulting protein (isoform 2) has a distinct C-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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