Thoc2 (NM_001191756) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1561623
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217663 representing NM_001191756 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCGCGACTGTGGTGGTTCCCGCAGAGTGGATAAAGAACTGGGAGAAATCAGGGAGAGGCGATT TTTTGCATTTATGCCGGATTCTCAGTGAAAACAAAAGCCATGACAGTTCGACCTACAGAGATTTTCAGCA AGCTCTCTATGAGTTGTCATACCATGTCATTAAGGGAAATTTAAAGCACGAACAGGCATCCAGTGTTCTT AATGACATTAGTGAATTCCGTGAGGATATGCCTTCTATCCTTGCTGACGTGTTCTGCATATTAGATATTG AGACAAATTGTTTAGAAGAAAAAAGCAAGAGAGACTATTTTACACAATTGGTATTAGCATGTTTGTATTT AGTTTCAGACACAGTTCTGAAGGAACGATTGGATCCAGAAACTTTGGAATCTTTAGGGCTTATCAAACAA TCACAGCAGTTCAACCAAAAGTCAGTTAAAATCAAGACAAAACTCTTTTATAAGCAGCAAAAATTCAATT TATTAAGAGAAGAAAATGAAGGTTATGCCAAGCTGATTGCTGAACTGGGGCAAGATTTATCTGGAACTAT TACTAGTGATTTAATATTAGAAAATATCAAATCTTTAATAGGATGCTTTAATCTGGATCCCAATAGAGTT TTGGATGTCATTTTAGAAGTATTTGAATGCAGGCCAGAACATGATGACTTCTTTATATCGTTATTAGAGT CTTACATGAGTATGTGTGAACCACAAACACTGTGTCATATTCTTGGATTCAAATTCAAGTTTTATCAGGA ACCAAGTGGTGAGACGCCCTCGTCTTTGTACAGAGTTGCAGCAGTCCTTCTGCAATTTAATCTCATTGAT CTAGATGATCTTTATGTACATCTCCTCCCAGCTGATAATTGTATTATGGATGAATACAAACGAGAAATTG TGGAAGCTAAGCAAATTGTAAGAAAACTCACAATGGTTGTACTGTCTTCTGAAAAACTTGATGAACGAGA CAAAGAAAAGGATAAAGATGACGAGAAAGTAGAAAAGCCACCTGACAACCAAAAACTTGGTTTGTTGGAA GCTTTGTTAAAGATTGGTGATTGGCAGCATGCACAGAACATCATGGATCAGATGCCTCCGTACTATGCAG CTTCACATAAGCTAATAGCTCTTGCTATTTGCAAGCTCATTCACATAACTGTCGAGCCTCTCTACCGAAG AGTTGGTGTTCCTAAAGGTGCTAAAGGCTCACCCGTTAGTGCTTTGCAAAATAAGAGAGCACCGAAGCAA GTAGAGAGCTTTGAAGATTTGAGGAGGGATGTATTCAATATGTTCTGCTACCTTGGTCCTCACCTCTCAC ATGATCCCATTTTATTTGCAAAAGTCGTGCGCATAGGCAAGTCATTTATGAAGGAGTTTCAGTCTGATGG AAGCAAACAAGAAGATAAAGAGAAAACGGAAGTTATCCTTAGCTGTTTACTTAGCATTACTGACCAGGTA TTACTTCCATCTCTCTCTTTGATGGACTGCAATGCTTGCATGTCTGAGGAACTGTGGGGAATGTTCAAGA CATTTCCCTATCAGCATAGATATCGTCTATATGGCCAGTGGAAGAATGAAACTTATAATGGGCACCCACT TTTAGTAAAAGTTAAAGCTCAAACAATAGACCGAGCCAAGTATATCATGAAGCGTCTAACCAAGGAAAAT GTGAAGCCTTCGGGAAGACAAATTGGCAAGCTGAGCCATAGTAATCCAACAATTTTGTTTGATTATATCT TGTCACAAATCCAGAAATATGATAACTTAATAACACCTGTAGTAGATTCATTGAAATACCTCACTTCATT GAATTATGATGTCTTGGCCTATTGTATCATTGAAGCTTTAGCTAATCCAGAAAAGGAGAGAATGAAACAT GATGACACAACCATCTCAAGCTGGCTTCAAAGTCTGGCAAGTTTCTGTGGTGCAGTATTCCGTAAATACC CAATCGATCTTGCTGGTCTTCTTCAGTATGTAGCCAATCAATTAAAGGCAGGCAAAAGTTTTGACCTACT AATATTGAAAGAAGTGGTACAAAAAATGGCAGGAATCGAAATTACAGAGGAGATGACGATGGAGCAGTTA GAGGCCATGACTGGTGGGGAGCAGCTGAAGGCTGAGGGTGGTTATTTCGGCCAGATCAGAAACACTAAAA AATCTTCTCAGAGATTAAAGGATGCACTACTGGACCATGATCTTGCTCTTCCTCTCTGTTTGCTCATGGC ACAACAGAGGAATGGGGTAATTTTTCAGGAGGGTGGAGAGAAACACTTGAAACTTGTGGGGAAGCTCTAT GACCAGTGTCATGATACCCTAGTACAGTTTGGTGGGTTTTTAGCATCAAATCTAAGTACAGAAGATTATA TAAAACGAGTGCCTTCAATTGATGTGCTCTGTAATGAATTTCATACACCTCACGATGCAGCATTTTTTCT GTCTAGGCCAATGTATGCACACCATATTTCATCAAAATACGATGAACTTAAAAAATCAGAAAAGGGAAGT AAACAGCAACATAAAGTCCATAAATACATTACATCATGTGAAATGGTGATGGCACCTGTCCATGAAGCAG TGGTCTCCTTGCATGTTGCCAAAGTCTGGGATGATATCAGCCCTCAGTTCTATGCTACATTTTGGTCGTT GACAATGTATGACCTTGCAGTTCCACATACCAGCTATGAAAGAGAAGTCAATAAACTTAAAGTCCAGATG AAAGCGATTGATGACAATCAGGAAATGCCTCCAAATAAAAAGAAGAAAGAAAAGGAGCGTTGCACGGCCC TTCAGGACAAGCTCCTTGAAGAAGAAAAGAAGCAGATGGAACATGTTCAGAGAGTACTTCAGAGACTGAA ACTAGAGAAAGACAACTGGCTTTTAGCAAAGTCTACCAAAAATGAGACCATCACAAAATTTTTACAGCTG TGTATATTTCCTCGATGCATTTTTTCAGCAATTGATGCTGTTTATTGTGCTCGTTTTGTTGAATTGGTAC ATCAACAGAAAACTCCAAATTTTTCCACACTTCTTTGCTATGATCGAGTTTTTTCTGACATAATTTACAC GGTTGCAAGCTGCACTGAAAATGAAGCTAGTCGGTATGGGAGATTCCTTTGCTGTATGTTAGAGACAGTG ACCAGGTGGCATAGTGATAGAGCCACTTATGAGAAGGAATGTGGAAACTATCCAGGATTCCTTACCATAT TACGGGCAACTGGATTTGATGGTGGAAACAAAGCTGATCAGTTAGATTATGAAAATTTTCGACATGTTGT CCACAAATGGCATTACAAATTAACCAAGGCCTCGGTTCATTGCCTTGAGACAGGCGAATATACTCACATC AGGAATATATTGATTGTGCTAACAAAAATACTTCCTTGGTACCCAAAAGTTTTGAATCTGGGTCAGGCTT TGGAAAGAAGAGTGAATAAAATATGCCAAGAAGAAAAAGAGAAGAGGCCAGATCTTTATGCATTGGCTAT GGGCTACTCGGGGCAGTTGAAAAGTAGAAAGTCACACATGATACCTGAAAATGAATTTCATCACAAGGAT CCTCCTCCAAGGAATGCTGTTGCCAGTGTACAAAATGGGCCTGGTGGTGGGACTTCTTCATCATCCATAG GAAGTGCGTCTAAATCAGATGAAAGCGGTGCTGAGGAGACTGATAAATCAAGGGAGAGATCTCAGTGTGG TACAAAAGCTGTTAATAAGGCTTCTAGTACCACACCAAAAGGGAATTCAAGCAATGGAAATAGTGGCTCT AACAGCAACAAAGCTGTTAAGGAAAATGACAAAGAAAAAGTAAAAGAGAAAGAAAAAGAGAAAAAAGAGA AGACTCCAGCTACAACTCCAGAGGCCAGGGTACTTGGTAAAGAAAGTAAAGAAAAACCGAAGGAAGAGCG GCCAAATAAAGATGACAAAGCAAGAGAGACAAAGGAAAGAACACCTAAATCTGACAAAGAGAAGGAGAAG TTCAAGAAGGAAGAAAAAGCAAAAGATGAGAAATTCAAGACCACTGTCCCCAGCGTAGAATCAAAATCGA CTCAAGAAAGGGAAAGAGAGAAGGAGCCATCCAGAGAAAGAGATGTAGCAAAGGAAATGAAGTCAAAGGA AAATGTTAAAGGAGGAGAAAAACCACCAGTTTCAGGGTCCTTGAAGTCACCTGTTCCCCGATCAGATATC TCGGAGCCTGACAGAGAACAAAAACGCCGCAAAATTGATACTCATCCTTCTCCATCACATTCCTCCACTG TAAAGGACAGTCTCATCGATCTCAAGGACTCTTCAGCAAAGCTCTACATTAACCATAATCCTCCACCACT GTCCAAGAGTAAGGAGAGAGAAATGGACAAGAAAGATTTGGACAAGTCAAGGGAAAGATCCAGAGAAAGA GAGAAAAAAGATGAAAAGGACAGGAAAGAGCGGAAAAGGGATCATTCAAACAATGACCGTGAAGTGCCAC CGGACATAACAAAGAGGCGGAAAGAGGAGAATGGAACAATGGGGGTTTCAAAACACAAAAGTGAAAGTCC ATGTGAGTCTCAATATCCAAATGAGAAAGACAAAGAGAAAAATAAGTCAAAATCTTCAGGCAAAGAAAAA GGCAGTAGTGATTCGTTTAAATCTGAGAAGATGGATAAAATCTCCTCTGGTGGCAAAAAGGAATCCAGGC ATGATAAAGAAAAGATAGAAAAGAAAGAAAAACGGGATAGTTCAGGAGGAAAAGAAGAGAAGAAACATCA TAAGTCCTCTGACAAGCACAGATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191756
Insert Size	4785 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191756.1, NP_001178685.1
RefSeq Size	7636 bp
RefSeq ORF	4785 bp
Locus ID	313308

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