Slit2 (NM_022632) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217651 representing NM_022632 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGGCATTGGCTGGCAGACACTGTCCCTATCTCTGGCGTTAGTGTTGTCGATCTTGAACCAGGTGG CGCCTCAGGCGTGCCCGGCCCAGTGCTCCTGTTCAGGCAGCACAGTGGACTGTCATGGGCTGGCACTGCG CAGTGTGCCCAGGAATATCCCCCGCAACACGGAGAGACTGGATTTGAATGGAAATAACATCACAAGGATC ACGAAGACAGATTTTGCGGGTCTCAGACACCTCAGAGTTCTTCAGCTCATGGAGAACAAGATCAGCACCA TCGAGAGGGGAGCATTCCAGGATCTTAAGGAGCTAGAAAGACTGCGTTTAAACAGAAATAACCTTCAGTT GTTTCCTGAGCTGCTGTTTCTTGGGACTGCGAAGCTCTACCGGCTTGATCTCAGTGAAAATCAGATTCAA GCAATTCCAAGGAAGGCTTTCCGTGGTGCAGTTGACATTAAAAATCTGCAGTTGGATTACAACCAGATCA GCTGCATTGAAGATGGGGCATTCCGAGCTCTGCGAGATCTGGAAGTGCTCACTCTGAACAATAACAATAT TACTAGACTTTCAGTGGCAAGTTTCAACCATATGCCTAAACTTAGGACATTTCGACTCCACTCCAACAAC CTATACTGCGACTGCCACCTGGCCTGGCTCTCGGACTGGCTTCGCCAAAGGCCACGGGTGGGCTTGTACA CTCAGTGTATGGGCCCATCCCACCTGAGGGGCCATAATGTAGCAGAGGTTCAAAAACGAGAGTTTGTCTG CAGTGATGAGGAAGAAGGTCACCAGTCATTCATGGCTCCCTCCTGCAGTGTGCTGCACTGCCCGATTGCT TGTACCTGTAGCAACAACATTGTAGACTGCCGAGGGAAAGGTCTCACTGAGATCCCCACAAATCTGCCTG AGACCATCACAGAAATACGTTTGGAACAGAACTCCATAAGGGTCATCCCTCCAGGAGCATTCTCACCATA CAAAAAGCTTCGACGACTAGACCTGAGTAATAACCAGATCTCGGAACTTGCTCCAGATGCCTTCCAAGGA CTGCGTTCTCTGAATTCCCTTGTCCTGTATGGAAATAAAATCACAGAACTCCCAAAAAGTTTATTTGAAG GACTGTTTTCCTTACAGCTACTATTATTGAATGCCAACAAGATAAACTGCCTTCGGGTAGATGCTTTTCA GGACCTGCACAACTTGAACCTTCTCTCCTTATACGACAATAAGCTTCAGACTGTTGCCAAGGGCACCTTC TCAGCTCTCAGAGCCATCCAAACTATGCATTTGGCCCAGAATCCTTTCATTTGTGACTGCCATCTCAAGT GGCTAGCGGATTATCTCCACACCAACCCAATTGAGACCAGCGGTGCCCGTTGCACCAGTCCCCGCCGCCT GGCTAACAAAAGAATTGGACAGATCAAAAGCAAGAAATTCCGTTGTTCAGGTACAGAAGATTATCGATCA AAATTAAGTGGAGACTGCTTTGCAGACTTGGCTTGTCCTGAAAAATGTCGCTGTGAAGGGACCACAGTAG ACTGCTCCAATCAAAAACTCAACAAAATCCCAGACCATATTCCCCAGTACACAGCAGAGCTGCGTCTCAA TAATAATGAATTCACAGTGTTAGAAGCCACGGGAATATTTAAGAAACTTCCTCAATTGCGTAAAATCAAC CTTAGCAACAATAAGATCACTGATATCGAGGAGGGGGCATTCGAAGGTGCGTCTGGTGTGAATGAGATTC TGCTTACCAGTAACCGTTTGGAAAATGTTCAGCATAAGATGTTCAAAGGATTGGAGAGCCTCAAAACATT GATGCTGAGAAGTAATCGAATAAGCTGTGTGGGAAACGACAGTTTCACAGGACTCGGTTCTGTGCGTCTG CTCTCTTTATATGACAATCAAATTACCACAGTTGCACCAGGAGCATTTGGTACTCTCCATTCATTATCTA CACTAAACCTCTTGGCCAATCCTTTCAACTGTAACTGTCACCTGGCATGGCTTGGAGAATGGCTCAGAAG GAAAAGAATTGTAACAGGAAATCCTCGATGCCAAAAACCCTACTTCTTGAAGGAAATACCAATCCAGGAT GTAGCCATTCAGGACTTCACCTGTGATGACGGAAACGATGATAATAGCTGCTCTCCACTCTCCCGTTGTC CTTCGGAATGTACTTGCTTGGATACAGTAGTACGATGTAGCAACAAGGGCTTGAAGGTCTTACCTAAAGG CATTCCAAGAGATGTCACAGAACTGTATCTGGATGGGAACCAGTTTACACTGGTCCCGAAGGAACTCTCC AACTACAAACATTTAACACTTATAGACTTAAGTAACAACAGAATAAGCACCCTTTCCAACCAAAGCTTCA GCAACATGACCCAACTTCTCACCTTAATTCTCAGTTACAACCGTCTGAGATGTATCCCTCCACGGACCTT TGATGGATTGAAATCTCTTCGTTTACTGTCTCTACATGGAAATGACATTTCTGTCGTGCCTGAAGGTGCC TTTGGTGACCTTTCAGCCTTGTCACACTTAGCAATTGGAGCCAACCCTCTTTACTGTGATTGTAACATGC AGTGGTTATCCGACTGGGTGAAGTCGGAATATAAGGAACCTGGAATTGCCCGCTGTGCCGGTCCCGGAGA AATGGCAGATAAATTGTTACTCACAACTCCCTCCAAAAAATTTACATGTCAAGGTCCTGTGGATGTTACT ATTCAAGCCAAGTGTAACCCCTGCTTGTCAAATCCATGTAAAAATGATGGCACCTGTAACAATGACCCGG TGGATTTTTATCGATGCACCTGCCCATATGGTTTCAAGGGCCAGGACTGTGATGTCCCCATTCATGCCTG TATCAGTAATCCATGTAAACATGGAGGAACTTGCCACTTAAAAGAAGGAGAGAATGATGGATTCTGGTGT ACTTGTGCTGATGGGTTTGAAGGAGAAAGCTGTGACATCAATATTGATGATTGCGAAGATAATGATTGTG AAAATAATTCTACATGCGTTGATGGAATTAACAACTACACGTGTCTTTGCCCACCGGAATACACAGGCGA ACTGTGTGAGGAAAAACTGGACTTCTGTGCACAAGACCTGAATCCCTGCCAGCATGACTCCAAGTGCATC CTGACGCCAAAGGGATTCAAGTGTGACTGCACTCCGGGATACATTGGTGAGCACTGTGACATCGACTTTG ATGACTGCCAAGATAACAAGTGCAAAAACGGTGCTCATTGCACAGATGCAGTGAACGGATACACATGTGT CTGTCCTGAAGGCTACAGTGGCTTGTTCTGTGAGTTTTCTCCACCCATGGTCCTCCCTCGCACCAGCCCC TGTGATAATTTTGATTGTCAGAATGGAGCCCAGTGTATCATCAGGGTGAATGAACCAATATGCCAGTGTT TGCCTGGCTACTTGGGAGAGAAGTGTGAGAAATTGGTCAGTGTGAATTTTGTAAACAAAGAGTCCTATCT TCAGATTCCTTCAGCCAAGGTTCGACCTCAGACAAACATCACACTTCAGATTGCCACAGATGAAGACAGC GGCATCCTCTTGTACAAGGGTGACAAGGACCACATTGCTGTGGAACTCTATCGAGGGCGAGTTCGAGCCA GCTATGACACCGGCTCTCACCCGGCTTCTGCCATTTACAGTGTGGAGACAATCAATGATGGAAACTTCCA CATTGTAGAGCTACTGACCCTGGATTCGAGTCTTTCCCTCTCTGTGGATGGAGGAAGCCCTAAAATCATC ACCAATTTGTCAAAACAATCTACTCTGAATTTCGACTCTCCACTTTACGTAGGAGGTATGCCTGGGAAAA ATAACGTGGCTTCGCTGCGCCAGGCCCCTGGGCAGAACGGCACCAGCTTCCATGGCTGTATCCGGAACCT TTACATTAACAGTGAACTGCAGGACTTCCGGAAAGTGCCTATGCAAACCGGAATTCTGCCTGGCTGTGAA CCATGCCACAAGAAAGTGTGTGCCCATGGCACATGCCAGCCCAGCAGCCAATCAGGCTTCACCTGTGAAT GTGAGGAAGGGTGGATGGGGCCCCTCTGTGACCAGAGAACCAATGATCCCTGTCTCGGAAACAAATGTGT ACATGGGACCTGCTTGCCCATCAACGCCTTCTCCTACAGCTGCAAGTGCCTGGAGGGCCACGGCGGGGTC CTCTGTGATGAAGAAGAAGATCTGTTTAACCCCTGCCAGGTGATCAAGTGCAAGCACGGGAAGTGCAGGC TCTCTGGGCTCGGGCAGCCCTATTGTGAATGCAGCAGTGGATTCACCGGGGACAGCTGTGACAGAGAAAT TTCTTGTCGAGGGGAACGGATAAGGGATTATTACCAAAAGCAGCAGGGTTACGCTGCCTGTCAAACGACT AAGAAAGTATCTCGCTTGGAGTGCAGAGGCGGGTGTGCTGGGGGGCAGTGCTGTGGACCTCTGAGAAGCA AGAGGCGGAAATACTCTTTCGAATGCACAGATGGATCTTCATTTGTGGACGAGGTCGAGAAGGTGGTGAA GTGCGGCTGCACGAGATGTGCCTCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_022632
Insert Size	4578 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_022632.2, NP_072154.2
RefSeq Size	7135 bp
RefSeq ORF	4578 bp
Locus ID	360272
Gene Summary	acts as a ligand for glypican-1, a heparan sulfate proteoglycan; may play a role in neurogenesis and midline development [RGD, Feb 2006]

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