Robo2 (NM_032106) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217649 representing NM_032106 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTCCTCTGATGTTTACACTACTATTGCTCTTTGGATTTCTCCGCATTCGGACTGACGGATNTCGTC TTCGTCAAGAAGACTTTCCCCCCAAGATNGTAGAACAACCTTCTGAAGTCATCGTCTCCAAAGGAAAACC CAACACTCCGAACTGGAAGCAAAAGGGCCGGCCATTCCCAACAATTGGAAAGGTACAAAGGATGGTTAAA CCGGGTTGGGACAAGACCAAGGATGATTCCAAGGTCACACAAGGATGCCTTCTGCCCAGCGGATCCTTAT TCTTTTTGCGAATTGTTCACGGACGCAGAAGTAAACCAGATGAAGGAACTTACGTTTGTGTTGCAAGGAA CTATCTTGGTGAAGCAGTGAGTCGCAATGCATCTCTGGAAGTAGCATTATTGCGAGATGACTTCCGGCAA AACCCCACAGATGTGGTAGTCGCAGCAGGAGAGCCTGCAATCTTGGAGTGCCAGCCACCACGGGGACACC CAGAACCAACCATCTACTGGAAAAAGGACAAAGTCCGAATTGATGAGAAGGAAGAGAGAATAAGTATCCG TGGTGGGAAGCTGATGATCTCCAATACTAGGAAAAGCGATGCTGGCATGTATACCTGTGTGGGAACCAAC ATGGTGGGAGAAAGGGACAGCGACCCTGCAGAGCTCACTGTCTTTGAACGACCCACGTTTCTCAGGAGGC CAATCAACCAGGTGGTGCTGGAGGACGAGCCTGCAGAATTCCGCTGTCAGGTCCAAGGAGATCCCCAGCC AACGGTGAGGTGGAAAAAGGATGACGCAGACTTACCAAGAGGAAGGTATGATATCAAGGACGACTACACC CTGAGAATCAAAAAGGCCATAAGTGCAGATGAAGGCACCTATGTGTGCATTGCTGAGAACCGGGTGGGAA AAGTGGAAGCTTCTGCCACCCTCACTGTCCGAGCTCCTCCACAGTTTGTGGTTAGGCCAAGAGATCAGAT CGTTGCTCAGGGCCGGACAGTGACATTCCCCTGTGAAACTAAAGGAAACCCACAGCCAGCTGTCTTCTGG CAGAAAGAAGGCAGCCAGAACCTACTTTTCCCGAATCAACCTCAGCAGCCCAATAGCCGCTGTTCAGTGT CGCCCACGGGGGACCTCACCATCACCAACATTCAGCGTTCAGATGCGGGTTACTACATCTGCCAGGCCCT AACTGTGGCAGGAAGCATTTTAGCTAAAGCACAGCTGGAAGTTACTGACGTTTTGACAGATAGACCTCCA CCCATAATCTTGCAAGGACCAATAAACCAAACACTTGCGGTAGATGGCACAGCATTACTGAAATGTAAAG CCACTGGACCTCTTCCTGTAATTAGCTGGCTAAAGGAGGGCTTTACTTTTCTGGGTAGAGATCCAAGAGC TACGATCCAAGACCAAGGAACACTGCAGATTAAGAATTTACGGATTTCTGATACTGGCACTTATACATGT GTGGCTACAAGTTCGAGTGGGGAGACTTCCTGGAGTGCAGTGCTGGATGTGACAGAGTCTGGAGCAACAA TCAGTAAAAATTATGATACAAATGACCTGCCCGGGCCACCATCCAAACCTCAGGTCACTGATGTTACGAA GAACAGTGTCACCCTATCCTGGCAGACAGGTACCCCTGGCGTTCTTCCAGCAAGCGCATATATCATTGAG GCTTTCAGCCAATCGGTGAGCAACAGCTGGCAGACAGTGGCAAACCATGTTAAGACGACTCTCTATACAG TCAGAGGACTAAGGCCCAACACAATCTATCTGTTCATGGTCAGAGCGATCAACCCCCAAGGTCTCAGTGA TCCAAGCCCCATGTCGGATCCTGTACGCACACAAGATATCAGTCCCCCAGCACAAGGCGTGGACCACAGA CAAGTGCAGAAGGAGTTAGGAGATGTCACTGTTCGTCTCCACAATCCAGTTGTCCTGACACCTACAACTG TTCAAGTCACATGGACGGTGGACCGACAACCCCAGTTTATTCAGGGCTACAGAGTGATGTACCGACAGAC TTCTGGTCTACAAGCTTCAACTGTGTGGCAGAATCTAGATGCCAAAGTCCCGACTGAGAGGAGTGCTGTC CTTGTGAATCTGAAAAAGGGGGTGACTTATGAAATTAAAGTCAGGCCATATTTCAATGAGTTCCAAGGAA TGGACAGTGAATCAAAAACAATCCGTACTACTGAGGAAGCTCCAAGTGCGCCTCCCCAGTCTGTCACGGT GCTGACCGTTGGAAGCCATAACAGCACAAGCATTAGTGTTTCCTGGGATCCTCCACCAGCCGACCACCAG AATGGAATCATACAGGAATACAAGATCTGGTGTCTGGGAAATGAAACGCGATTCCATATCAATAAAACTG TGGATGCAACCATTCGCTCTGTAGTAATAGGTGGCTTGTTCCCTGGAATTCAGTACCGGGTGGAAGTGGC AGCTAGTACAAGTGCAGGGGTTGGAGTAAAAAGTGAACCACAGCCAATAATCATTGGGGGACGTAACGAA GTTGTCATTACTGAAAACAATAACAGCATAACTGAGCAAATCACTGATGTCGTGAAGCAACCAGCATTTA TAGCTGGCATCGGTGGTGCCTGCTGGGTAATTTTGATGGGTTTTAGCATCTGGTTATACTGGAGAAGAAA GAAGAGAAAGGGACTTAGCAATTATGCTGTAACATTTCAAAGAGGAGATGGAGGACTAATGAGCAATGGG AGCCGTCCAGGTCTTCTAAATACTGGCGACCCCAATTATCCATGGCTTGCTGACTCATGGCCAGCCACGA GTTTGCCAGTCAACAACAGCAATAGTGGCCCAAATGAAATCGGAAATTTTGGGCGTGGAGATGTGCTGCC TCCAGTCCCAGGCCAAGGGGATAAAACAGCAACAATGCTCTCAGATGGAGCTATTTATAGTAGCATTGAC TTCACTACCAAGACCACTTACAACAGTTCCAGCCAAATAACGCAGGCCACCCCATACGCCACTACCCAGA TCCTGCATTCCAACAGCATCCATGAACTGGCGGTTGATCTTCCTGATCCACAGTGGAAAAGCTCAGTTCA ACAGAAATCAGACCTCATGGGATTTGCTTATTCACTACCTGATCAGAACAAGGGGAACAATGCCTTACTT TACATCCCTGACTACCGATTGGCTGAGGGATTGTCTAATAGAATGCCACACAACCAGTCACAGGATTTCA GCACCACCAGCTCTCACAACAGCTCAGAAAGGAGTGGCAGTCTCTCAGGTGGGAAAGGTGGAAAAAAGAA GAAAACTAAAAATTCTTCAAAGGCGCAAAAAAACAACGGATCCACTTGGGCTAATGTCCCTCTACCTCCT CCTCCTGTCCAGCCCCTTCCGGGTACAGAGCTCGGACACTATCCTGCGGAACAGGAAAATGGCTATGACA GTGATAGCTGGTGCCCACCATTACCAGTGCAAACCTATTTGCATCAGGGAATGGAAGACGAGTTGGAAGA GGATGAAGATCGCGTCCCAACACCTCCTGTTCGCGGTGTGGCCTCTTCACCCGCTATCTCCTTTGGGCAG CAGTCCACTGCCACTCTTACACCATCCCCACGGGAGGAGATGCAACCCATGCTGCAAGCTCACTTGGATG AGTTAACCAGGGCCTATCAGTTTGATATAGCAAAACAAACATGGCACATTCAAAGCAATACTCCACCTCC ACAACCCCCAGTTCCACCATTAGGTTACGCGTCCGGAGCCTTGATTTCTGACTTGGAGACAGATGTTCCA GATGAGGATGCTGATGATGAAGAGGAACCATTAGAAATTCCCAGGCCCCTCAGAGCACTAGACCAGACTC CTGGTTCCAGTATGGACAATCTAGACAGCTCTGTAACAGGAAAAGCCTTTACTTCCTCTCAAAGGCAGCG GCCCACCAGCCCATTTTCTACAGACAGTAACACCAGTGCTGCCCAGAATCAAAGCCAGAGGCCTCGGCCC ACCAAAAAACACAAGGGAGGGCGGATGGACCCACAGCCAGTGTTACCTCATCGGAGGGAAGGGATGCCAG ATGATCTTCCCCCACCACCAGATCCCCCACCAGGTCAGGGTTTAAGGCAGCAGATAGGCCTGAGCCAACA CTCTGGTAACGTGGAAAGCTCAACAGAGAGGAAAGGAAGCTCTCTAGAGAGACAACAAGCAGCCAACTTA GAAGACACAAAGAGCTCATTGGATTGTCCGGCTAAAACTACTGTAGAGTGGCAGCATCAAACCCAGGACT GGATAAACTCTACAGAGCGCCAAGAAGAAACACGGAAAGCCCCACACAAACCAGGGGTTGGATCAGAGGA GTCCCTGGTGCCCTACAGCAAGCCCAGCTTCCCATCTCCAGGCGGGCACAGCTCATCCGGAACAGCCTCC TCTAAAGGGTCCACTGGTCCTCGGAAGGCTGAGATTCTGAGGGGCAGCCACCAGCGGAATGCCAATGACC TTCTCGACATTGGATATGTGGGCTCAAATAGTCAAGGGCAGTTTACAGGTGAATTGTAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_032106
Insert Size	4539 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_032106.2, NP_115289.1
RefSeq Size	8977 bp
RefSeq ORF	4539 bp
Locus ID	84409
Gene Summary	repulsive axon guidance receptor for the slit protein ligands; plays a role in axon guidance and repulsion [RGD, Feb 2006]

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