Cdk13 (NM_001271295) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Cdc2l5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217648 representing NM_001271295 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGAGCAGCTCGGACACGGCGCTGGGGGGAGGCGGGGGCCTGAGCTGGGCCGAGAAGAAGTTGGAGG AGCGCCGCAAGCGGAGGCGATTCCTGTCCCCTCAGCAGCCGCCGCTGCTGTTGCCGCTCCTGCAGCCGCA GCTCCTGCAACCGCCGCCGCCCCCGCCGCCTCTGCTCTTCCTGGCTGCCCCCGGCGCGGCCGCCGCCGCA GCCGCCGCCGCCGCGGCCTCCTCCTCCTGCTTCAGCCCGGGCCCTCCTTTGGAGGTCAAGCGGCTGGCGA GAGGCAAGAGGCGCCCCGGAGGGCGGCAGAAGCGGCGCCGCGGGCCCCGCGCCGGGCAGGAGGCGGAGAA GCGCCGGGTCTTCTCGCTGCCCCAGCCGCAGCAGGACGGCGGTGGCGGTGCCAGTAGCGGCGGGGGTGTG ACCCCGCTGGTGGAGTATGAGGATGTAAGCTCCCAGTCCGAGCAGGGGCTGCTGCTGGGGGGCGCCAGCG CGGCAACGGCGGCGACGGCTGCCGGGGGAACGGGGGGCAACGGCGGGAGTCCGGCCTCCTCCTCCGGCAC GCAGAGGCGCGCGGAGGGGTCGACGCGCAGGCCGCGCCGGGACCGCCGCAGCAGCAGCGGTCGCAGTAAG GAGCGCCATCGCGAGCACCGGCGGCGGGACGGGACGCGCAGTGGCAGCGAGGCCTCAAAGGCCCGCAGCC GCCACGGACACAGCGGCGAGGAGCGGGCCGAGGCCGCCAAGAGCGGCAGTAGCAGCAGCAGCGGCGGCCG CCGCAAAAGTGCATCGGCTACGTCCAGCAGTAGTAGCAGCCGCAAGGATCGGGACCTCAAGGCCCATCGC AGCCGGACTAAGTCGTCCAAGGAGCCGCCCTCGGCCTACAAGGAGCCGCCCAAGGCCTACCGGGAGGACA AGAGCGAACCGAAGGCCTACAGGCGGCGGCAGCGGTCCCTGAGCCCGCTGGGAGGCCGGGACGAAAGCCC GGTGTCCCACAGGGCCTCGCAGAGCCTCCGGAGCCGCAAGTCCCCCAGCCCGGCGGGAGGTGGCAGCAGT CCCTATTCCCGGCGGTTGCCGCGCTCCCCGAGCCCCTACAGCCGGCGCCGCTCGCCCAGCTACAGCCGCC ACAGCTCCTACGAGCGGGGCGGCGACGTATCCCCCAGCCCGTACAGCAGCAGCAGCTGGCGGCGCTCGCG CAGTCCCTACAGTCCAGTACTCAGACGATCTGCCAAATCCCGAAGCAGAAGCCCCTATTCATCTAGGCAC TCAAGATCTCGTAGCAGGCACAGATTGTCTAGATCCCGAAGTCGTCATTCAAGCATTTCTCCTAGCACAC TAACTCTGAAGAGTAGCCTGGCAGCTGAATTAAACAAGAATAAAAAGGCACGAGCTGCAGAGGCAGCAAG AGCTGCAGAGGCAGCCAAAGCTGCAGAAGCTGCTAAGGCTGCTGAGGCAGCTGCCAAAGCTGCAAAAGCC TCTAGTGTTTCTACACCTACCAAGGGGAACACAGAAACTGGTGCCAGTGTCTCACAGACAAACCATGTGA AGGAAGTCAAAAAACTTAAAACTGAGCATGCACCTTCTCCTTCAAGTGGTGGGACTGTCAAAAGTGACAA GACAAAAACAAAGCCACCGCTTCAAGTAACAAAAGTAGACAATAATTTGATTGTAGAGAAAGCTACCAAG AAAACAGTTGTTGGGAAAGAGAGTAAACCTGCTGCTACAAAGGAAGAACCAGTGTCTACTAAAGAGAAAA CCAAGCCACTCACACCAAGTGTAGGAGCCAAGGAGAAGGAGCAACATGTGGCTTTAGTGACCTCTACATT ACCACCATTACCTTTGCCTCCCATGTTGCCTGAAGATAAAGATGCTGATAGTTTAAGAGGCAATATTTCT GTAAAAGCAGTTAAAAAAGAAGTAGAAAAGAAACTCCGGTGTCTGCTTGCTGATTTACCATTGCCCCCTG AGTTACCAGGAGGAGATGATCTTTCCAAGAGTCCAGAGGAGAAGAAAACAGCAACACAGTTACATAGCAA AAGAAGGCCTAAAATATGTGGGCCTCGCTATGGTGAAATCAAAGAAAAAGATATTGACTGGGGGAAACGC TGCGTGGATAAATTTGATATCATCGGAATTATTGGAGAAGGTACTTATGGACAAGTTTACAAAGCCAGGG ACAAAGACACTGGAGAAATGGTAGCCTTAAAGAAAGTACGTCTGGATAATGAAAAGGAGGGTTTCCCAAT TACAGCAATTAGAGAAATTAAAATTCTTCGGCAACTTACCCATCAGAGTATCATCAATATGAAGGAAATA GTGACTGATAAAGAAGATGCTTTGGATTTTAAGAAAGACAAAGGTGCATTTTACCTGGTGTTTGAATATA TGGACCATGATCTGATGGGACTGCTGGAGTCAGGCTTGGTTCATTTTAATGAAAACCATATAAAATCTTT TATGAGACAGCTCATGGAAGGCCTGGATTATTGTCATAAGAAGAACTTTTTGCACAGAGATATTAAATGT TCAAATATCCTTCTAAATAATAGAGGACAGATAAAACTTGCAGATTTTGGACTTGCTCGTTTATATAGCT CAGAAGAAAGCCGTCCATATACTAACAAGGTCATTACTTTGTGGTATCGTCCACCTGAACTGCTCTTGGG AGAAGAACGATATACACCGGCCATTGATGTATGGAGCTGTGGATGTATCCTTGGGGAACTCTTCACTAAA AAGCCTATATTTCAAGCAAACCAGGAACTTGCACAACTAGAGCTAATAAGTCGTATATGTGGGAGTCCGT GTCCTGCAGTATGGCCTGATGTAATCAAACTGCCATATTTCAACACCATGAAACCAAAGAAGCAATATCG GCGGAAGTTAAGAGAAGAGTTTGTTTTTATCCCTGCAGCTGCACTCGACTTATTTGATTACATGCTTGCC TTGGATCCCAGTAAGCGCTGCACTGCCGAGCAGGCTCTTCAGTGTGAGTTCCTGCGAGACGTGGAACCCT CCAAAATGCCTCCACCAGACCTTCCTTTGTGGCAAGATTGTCATGAATTATGGAGTAAAAAGAGAAGAAG ACAGAAACAGATGGGCATGACTGATGATCTTTCCACAATCAAAGCCCCCAGAAAGGACTTGTCTCTGGGC TTAGATGACAGCAGAACTAGCACGCCCCAGGGTGTGCTGCCACCTGCACAGCTAAAGTCTCAGAACAGCT CCAGTGGAGCACCTGTAATAACAGGCCCTGGACAGCCGTTAAACCACAGTGAATTGGCAATTCTTCTAAA CCTACTACAATCTAAATCAAGTGTTAATATGGCTGATTTTGTCCAAGTGTTGAACATTAAGGTAAACTCT GAGACTCAACAGCAGCTAAATAAAATAAACCTTCCTGCTGGAATTTTGGCAACAGGTGAAAAACAGACAG ATCCATCAACACCACAACAGGAGTCTTCAAAATCATTGGGAGGAGTTCAGCCTTCACAGAGCATCCAGCC TAAAGTGGAAACTGACGCTGCGCAGGCTGCAGTGCAGAGTGCATTTGCAGTCCTCCTGACTCAGTTAATA AAGGCCCAGCAGTCAAAACAGAAAGATGCCATGCTGGAGGAGAGGGAGAATGGATCAGGACATGACGCTC CAGTGCAACTCAGGCCGCCTCCAGAACCCAGCACTCCTGCCTCTGGGCAAGATGACCTCATCCAACACCA AGACAGGAGGATATTGGAGCTGACACCAGAGCCAGAGCGGCCTCGGATTCTGCCTCCTGATCAACGACCT CCTGAACCTCCTGAACCACCACCAGTCACTGAGGAGGACCTGGATTATCGGACAGAAAACCAGCATGGAC CTACTACTAGTTCTTCATTAACTGACCCACATGCTGGAGTGAAGGCAGCCCTCTTACAGCTGCTTGCTCA ACATCAGCCCCAGGATGATCCCAAAAGAGAAGGTGGTATTGATTATTCCACAGGAGACACGTACGTGCCC AGTTCAGACTATAAAGACAACTTTGGAGCGTCTTTCTCTGCCGCCCCTTATGTGAGCAGTGATGGTCTGG GGAGTAGCTCTGCTGCTGCACCGTTGGAGGCGCGCAGTTTCATCGGAAACTCAGATATTCAGTCTCTGGA TAACTACAGTACTGCTTCATCTCACACTGGTGGTCCACCTCAGACTTCTGCCTTCACCGAGTCATTTCCC AGTTCAGTAGCTGGATACGGAGACATTTACCTCAATGCTGGTCCCATGCTGTTTAGTGGAGACAAGGACC ATAGATTTGAGTACAGCCATGGTCCTATCACGGTCCTCACAAACAGCAGTGACCCTTCCACAGGGCCGGA GAGTACTCACCCCTTGCCAGCAAAGATACACAACTATAACTATGGTGGTAACTTACAGGAAAATCCAGGT GGCCCCAGCCTCATGCATGGACAGACCTGGACTTCTCCTGCCCAAGGACCTGGATACTCACAAGGATACA GGGGACACATTAGCACATCAGCTGGCAGAGGTCGAGGCAGAGGGTTACCATACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271295
Insert Size	4536 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271295.1, NP_001258224.1
RefSeq Size	6124 bp
RefSeq ORF	4536 bp
Locus ID	306998

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