Arhgap35 (NM_001271132) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Grlf1; p190RhoGAP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217647 representing NM_001271132 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGATGGCAAGAAAGCAAGATGTCCGAATCCCCACCTACAACATCAGTGTGGTGGGATTGTCCGGCA CTGAGAAGGAGAAAGGCCAGTGCGGCATTGGGAAGTCTTGTCTATGTAACCGCTTTGTGCGCCCAAGTGC TGATGAGTTTCACCTGGACCACACTTCTGTCCTCAGCACCAGTGACTTTGGTGGGCGGGTGGTCAATAAT GACCATTTTCTGTACTGGGGAGAAGTTAGCCGCTCCCTGGAGGACTGTGTGGAATGTAAGATGCACATTG TGGAGCAGACTGAGTTTATTGACGATCAGACTTTTCAACCTCATCGAAGCACGGCACTGCAGCCCTACAT CAAGAGAGCCGCAGCCACCAAGCTTGCTTCAGCTGAAAAACTCATGTATTTTTGCACTGACCAGCTAGGG CTGGAGCAAGACTTTGAGCAGAAACAGATGCCAGATGGGAAGCTGCTGGTTGACGGTTTCCTTCTGGGCA TCGATGTTAGCAGGGGCATGAACAGGAACTTCGATGACCAGCTCAAGTTTGTCTCCAATCTCTACAATCA GCTTGCAAAAACAAAAAAGCCCATAGTAATAGTCCTGACCAAGTGTGATGAGGGTGTTGAGCGGTACATT AGAGATGCACATACTTTTGCCTTAAGCAAAAAGAACCTCCAGGTTGTAGAGACCTCTGCAAGGTCCAATG TGAATGTGGACTTGGCTTTCAGCACCTTAGTGCAACTCATTGATAAGAGTCGAGGGAAGACGAAAATCAT CCCTTACTTTGAAGCTCTCAAGCAGCAGAGTCAGCAGATAGCTACAGCAAAGGACAAGTATGAGTGGTTG GTGAGCCGCATTGTGAAAAGTCACAATGAGAACTGGCTGAGTGTCAGCCGAAAGATGCAGGCCTCCCCTG AGTACCAGGACTATGTCTATCTGGAAGGGACACAGAAAGCCAAGAAGCTCTTCCTGCAGCACATTCACCG CCTCAAGCATGAGCATATTGAGCGCCGGAGAAAGCTGTACCTGGCAGCCCTGCCATTGGCTTTTGAAGCC CTCATACCTAATCTAGATGAAGTAGACCACCTGAGCTGCATTAAAGCAAAAAAGCTGTTAGAGACTAAGC CAGAGTTCTTAAAGTGGTTTGTTGTACTTGAAGAGACACCATGGGATGAAACCAGCCACATTGACAACAT GGAAAATGAGCGGATTCCCTTTGACTTGATGGATACTGTCCCTGCTGAGCAGTTGTATGAGACCCACTTG GAGAAGCTGAGGAATGAGAGGAAGAGAGCTGAGATGCGAAGGGCTTTCAAAGAAAACCTGGAGACCTCTC CTTTCATAACTCCTGGGAAACCTTGGGAAGAAGCTCGTAGTTTTATTATGAATGAAGACTTCTACCAGTG GCTGGAAGAATCTGTGTACATGGACATCTATGGCAAGCACCAAAAGCAGATTATAGACCGGGCAAAGGAA GAGTTCCAAGAGTTGCTTTTGGAGTATTCAGAATTGTTTTATGAGCTGGAGCTGGATGCTAAACCCAGTA AGGAAAAGATGGGTGTTATCCAGGATGTTCTGGGTGAAGAGCAACGATTTAAAGCATTGCAGAAACTCCA AGCAGAGCGTGATGCCCTCATTCTGAAGCACATTCATTTTGTGTACCACCCAACAAAGGAGACATGCCCA AGCTGTCCAGCTTGCGTAGATGCTAAGATTGAACATTTGATCAGTTCTCGCTTTATCCGACCATCTGATA GGAATCAGAAGAACTCTTTGTCTGACCCCAATATTGATAGGATCAATTTGGTTATTTTAGGCAAAGATGG CCTTGCCCGAGAGTTAGCCAATGAAATTCGAGCTCTTTGTACAAATGATGACAAGTATGTAATAGATGGT AAAATGTATGAGCTTTCTCTGAGGCCAATAGAGGGGAATGTTCGGCTTCCTGTGAACTCTTTCCAGACAC CAACCTTCCAACCCCATGGCTGTCTCTGCCTTTACAATTCAAAGGAGTCGCTGTCCTATGTGGTGGAGAG TATAGAGAAGAGCAGAGAATCTACACTGGGCAGGCGGGATAATCACTTAGTCCACCTCCCCTTGACTTTA ATTTTAGTTAACAAGAGAGGGGACACAAGTGGAGAGACTCTGCACAGCTTAATACAGCAAGGCCAGCAGA TTGCTAGCAAACTTCAGTGTGTCTTTCTTGATCCTGCGTCTGCTGGCATTGGTTATGGACGCAACATTAA CGAGAAGCAGATCAGTCAAGTTCTGAAGGGACTCCTGGACTCTAAGCGCAACTTAAACCTGGTTAGTTCT ACTGCTAGTATCAAAGATTTGGCTGATGTGGACCTTCGAATTGTCATGTGTCTCATGTGTGGTGATCCTT TTAGTGCAGATGACATTCTCTCTCCTGTCCTGCAGTCCCAAACTTGTAAATCTTCCCACTGTGGGAGCAG CAACTCTGTTTTACTTGAACTTCCAATTGGAGTACACAAGAAGCGCATTGAGCTGTCTGTTCTTTCATAC CATTCCTCATTTAGCATCCGAAAGAGCCGGTTGGTTCATGGGTACATTGTTTTTTATTCAGCCAAACGTA AGGCCTCCTTGGCAATGTTACGTGCCTTTCTTTGTGAAGTGCAGGATATTATCCCCATCCAGCTTGTCGC ACTCACTGATGGCGCTATAGATGTCCTGGACAATGACTTAAGTCGAGAGCAGCTAACAGAGGGAGAGGAA ATTGCACAAGAAATTGATGGGAGATTCACAAGCATCCCTTGTAGCCAGCCCCAGCATAAACTCGAGCTCT TCCATCCCTTTTTTAAAGATGTGGTGGAGAAAAAGAACATAATCGAGGCCACACACATGTACGATAATGT GGCTGAGGCCTGCAGCACCACTGAGGAGGTATTCAACTCCCCCAGGGCTGGGTCACCCCTCTGCAATTCA AACTTACAGGACTCAGAAGAAGATGTGGAGCCTCCATCGTACCACCTTTTTCGGGAAGATGCGACATTGC CCTCCCTGTCCAAAGATCATTCCAAGTTCTCAATGGAGCTGGAGGGAAACGACGGGCTGTCTTTCATAAT GAGCAACTTTGAGAGTAAACTGAACAACAAAGTACCTCCACCAGTCAAACCAAAGCCTCCTGTGCATTTT GAGATCACAAAAGATCTTTCTTACTTAGACCAAGGTCATCGGGAGGGACAGAGGAAGTCTATGTCTTCTA GCCCCTGGATGCCTCAGGATGGATTTGATCCTTCTGACTACGCAGAGCCCATGGATGCTGTGGTCAAGCC AAGGAATGAGGAAGAAAACATATACTCAGTGCCCCACGACAGCACCCAGGGCAAGATCATTACCATTCGG AACATCAACAAAGCCCAGTCCAATGGCAGTGGCAATGGTTCTGACAGTGAGATGGACACAAGCTCTCTAG AGCGAGGCCGCAAAGTATCTGCAGTGAGTAAGCCTGTGCTGTACAGGACGAGATGCACCCGCCTGGGGCG GTTTGCTAGTTACCGCACCAGCTTCAGTGTTGGGAGTGATGATGAGCTGGGACCCATCCGAAAGAAAGAG GAGGACCAGGCATCCCAAGGTTATAAAGGGGACAATGCTGTCATTCCTTATGAAACAGATGAGGACCCCA GGAGGAGGAATATCCTTCGAAGTCTAAGGAGGAACACCAAGAAACCAAAGCCCAAACCCCGACCATCCAT CACAAAGGCAACCTGGGAGAGTAACTATTTTGGGGTGCCTTTAACAACAGTGGTGACTCCAGAGAAGCCG ATACCCATTTTCATTGAAAGATGCATTGAGTACATTGAAGCCACAGGACTAAGCACAGAAGGCATCTACC GGGTCAGCGGAAACAAGTCAGAAATGGAAAGTTTGCAAAGACAGTTTGATCAAGATCACAATCTGGACCT GGCAGAGAAAGACTTCACTGTGAACACTGTGGCAGGGGCCATGAAGAGTTTTTTCTCGGAGCTACCAGAC CCCCTGGTACCATACAGCATGCAGATTGACTTGGTGGAAGCTCACAAGATCAACGACAGGGAGCAGAAGC TGCATGCTCTGAAGGAAGTGCTGAAGAAGTTCCCTAAGGAGAACCATGAAGTCTTCAAATATGTCATCTC CCACCTGAACAGAGTCAGCCACAACAACAAGGTGAATCTTATGACCAGTGAGAACCTGTCCATCTGCTTC TGGCCCACGTTGATGCGGCCTGACTTCAGCAGCATGGACGCACTCACAGCCACTCGATCCTACCAGACCA TCATCGAGCTCTTCATCCAGCAGTGCCCCTTCTTCTTCTACAACCGGCCGATCAGTGAGCCACCGGGGGC TGCGCCTGGCTCCCCTTCAGCCATGGCACCCACTGTCCCCTTCCTCACCTCTACACCTGCTACCAGTCAG CCATCACCTCCCCAGTCACCTCCTCCAACCCCTCAGTCCCCAATGCAGCCATTGCTCTCCTCTCAGCTCC AAGCCGAACACACGCTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271132
Insert Size	4500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271132.1, NP_001258061.1
RefSeq Size	8364 bp
RefSeq ORF	4500 bp
Locus ID	306400
UniProt ID	P81128
Gene Summary	Rho GTPase-activating protein (GAP). Binds several acidic phospholipids which inhibits the Rho GAP activity to promote the Rac GAP activity (PubMed:9852136, PubMed:20439493). This binding is inhibited by phosphorylation by PRKCA (By similarity). Involved in cell differentiation as well as cell adhesion and migration, plays an important role in retinal tissue morphogenesis, neural tube fusion, midline fusion of the cerebral hemispheres and mammary gland branching morphogenesis (PubMed:9852136, PubMed:20439493). Transduces signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP) (By similarity). Transduces SRC-dependent signals from cell-surface adhesion molecules, such as laminin, to promote neurite outgrowth. Regulates axon outgrowth, guidance and fasciculation (By similarity). Modulates Rho GTPase-dependent F-actin polymerization, organization and assembly, is involved in polarized cell migration and in the positive regulation of ciliogenesis and cilia elongation (By similarity). During mammary gland development, is required in both the epithelial and stromal compartments for ductal outgrowth (By similarity). Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'; this function is however unclear and would need additional experimental evidences (By similarity).[UniProtKB/Swiss-Prot Function]

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