Cpamd8 (NM_001271376) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1566313
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217640 representing NM_001271376 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGAAGAACAGAGAGGCTCACCTGTGCCTTTTTTCAGTTCTTCTTGCCCTCCTGCCTTCTGCTTCCT CGTTCAATGGAAACTCGAAGTATATGGTGCTGGTCCCGTCCCAGCTCTACACCGACACCCCTGAGAAAAT CTGCCTGCATCTATACCATCTGAATGAGACAGTGACTGTGACAGCTTCCTTGATATCTCAAAGGGGAACG AGAAAACTGTTCGATGAGCTTGTGGTTGACAAGGACTTGTTCCACTGTCTTTCCTTCACTATCCCTAGAT TGCCCTCTTCTGAAGAGGAGGAGTCACTCGACATCAACATAGAAGGGGCAAAGCATAAATTCAGCAAAAG GCGTGTGGTGCTTGTGAAGAACAAAGAAAGTGTTGTCTTTGTCCAGACTGATAAGCCCGTGTACAAGCCA GGACAGTCAGTTAAATTTCGGATTGTCTCTATGGATAAAAATCTACACCCCCTGAATGAGTTGTTTCCTC TGGCTTACATTGAGGATCCCAAAATCAACCGAATTATGCAGTGGCAGGACATTAAGACAGAGAATGGGCT TAAGCAATTGTCCTTCAGCCTGTCAGCAGAGCCCATTCAGGGCCCCTACAAGATAGTCATTCTCAAACAG TCAGGAGTGAAGGAAGAGCATTCCTTCACCGTCATGGAATTCGTGCTTCCCAGATTTGGTGTCGATGTGA AGGTCCCAAACGCCATCTCTGTGTATGATGAGATAATCAGTGTGACTGCCTGTGCGACATATACCTATGG GAAACCTGTCCCGGGACGTGTAAAGATAAGTCTTTGCCATGGAAATCCTTCCTTTAGATCTCAGACAAAG TCTGCATGCAAAGAAGAGGACTCGGAGCTAGACAACAACGGCTGCAGCACACAAGAAGTGAACATCACTG AGTTCCGATTGAAGGAAAATTACCTCAAAGTGCGCCAGGCTTTCCATGTGAATGCAACTGTTACAGAAGA AGGGACAGGGTCAGAGTTCAGTGGATCTGGAAGAATTGAAGTCAAAAGAACCAGAAACAAATTCCTATTT CTGAAAGCAGATTCCCACTTCAGACATGGGATCCCATTCTTTGTGAAGGTCCGCCTAGTGAATATCAAGG GAGATCCTATCCCAAATCAGCAGGTCTTCATCAAAGCACAAGGAGCTGGCTTCACCAATGCTACTACCAC TGATCAGCATGGCTTGGCCAAGTTCTCCATAGATACCAGCGGCATCTCACGATATATCCTGAATATCAAA GTCTACCACAAGGAGGAAAATTCATGCATCCATTCCTCTTGCACAGCAGAGAGACACGCAGAGGCACATC ATACGGCCTATGCTGTTTACTCCCTCAGCAAGAGCTACATCTACCTTGACACAGAAGCTGGCGTCTTGCC CTGCAACCAGAGCCACACAGTTCAGGCACATTTTATTCTGAAGGGGCAGGTCTTGGGAGTGCTGCCACAG ATTGTTTTCCACTACCTGGTCATGGCCCAGGGCAGCATCCTCCAGACTGGAAACCACACTCATCAAGTGG AGCCAGGAGAATCTCCAGTACAAGGCAACTTCGCCTTGGAGATCCCTGTGGAGTTCAGCATGGTTCCCAT GGCTAAAATGCTCATCTACACCATCTTGCCTGATGGAGAAGTGATTGCAGATTCTGTAAAGTTTCAAGTT GAAAAATGTCTTCGTCATGAAGTGCACCTGAGTTTCAGCCCATCCCAAAGTCTTCCAGCCTCACAAACCC ATATGCGGGTTACAGCTTCTCCTCAGTCCCTCTGTGGCCTGAGAGCTGTGGACCAAAGCGTGCTGCTGCT GAAGCCTGAGGCTGAGCTCTCCCCTTCCTTGATATATGATCTACCAGGTATGCAAGAGAGCAAGTTCATT CCACGTTCCCACTATCCATTTGAAGACGAAGATGGTTGTGTGATATACCGACCAAGAGTCATTGGAAAGC TCACATACTCAGTACCATATGGACGAGAGAAGGATGTCTTCAGATACATGAGGGACACGGGTTTAACGGC ACTCACCAACTTGAAACTTCGTACATTTTGTTTTGACTATGACATGGTTCCATTAGCAGTACCTGCTGCC CGATCTAGTGATTTATTTAACAGCGACGGTTATGGAAGTTGGCGCTCAGCAAGAGTAAAAAGTCTACCTC CTAAAAAGGCACCACATAAAGATCCACCACCTAAAGATCCAGTTATTGAGACCATTAGGAATTATTTTCC TGAAACCTGGGTCTGGGATTTAGTCACAGTAAACTCCTCAGGAGCAGCTGAATTGGAAATGACAGTCCCT GACACCATCACTGAATGGAAGGCCGGAGCCCTCTGCCTGTCCAATGACACTGGTCTTGGCCTCTCTTCTG TGGCAACTCTCCAAGCCTTCCAACCTTTCTTTGTGGAGCTCACAATGCCCTACTCTGTGATCCGTGGAGA AGCCTTCACGCTCAAGGCCACTGTGATGAACTACCTCCCTACAAGCTTGCCGATGGCAGTGCAGCTGGAA GCTTCTCCTGATTTCACAGCTGTCCCAGTGGGAAACGACCAAGATTCTTACTGCCTTGGTGCCAATGGAA GGCACACCTCATCCTGGTTGGTAACTCCCAAATCTTTAGGGAATGTGAATTTCTCTGTGTCTGCGGAAGC ACAACAGTCCCCAGAGCCTTGTGGTTCTGAGGTGGCCACAGTGCCTGAAACCGGGAGAAAGGACACAGTC GTCAAAGTCTTGATAGTTGAGCCTGAAGGAATCAAGAGAGAGCATACTTTCAGCTCACTGCTCTGTGCAT CAGATGCTGAGCTATCAGAAACATTGTCTCTGCTGCTTCCACCAACAGTAGTGAAAGATTCAGCCAGAGC ACATTTCTCTGTGATGGGTGATATCTTGAGTTCAGCCATAAAAAACACACAAAATCTTATCCAAATGCCC TATGGCTGTGGGGAGCAGAACATGGTCCTTTTTGCTCCGAGCATCTATGTACTGAAATATCTGAATGAAA CCCAACAGCTGACAGAGAAGATCAAGTCCAAGGCCCTTGGCTACCTCAGAGCTGGTTATCAGAGGGAACT GAACTACAAACACAAGGATGGCTCCTACAGTGCCTTTGGGGATCACGATGGCAAGGGTCAAGGAAACACT TGGCTCACAGCCTTTGTGCTCAAGTCTTTTTCCCAAGCTCAAGCCTTCATCTTCATTGACGAATCACACA TCACCGATGCCTTCACCTGGCTCTCCCATCAGCAGAAGGACAGTGGCTGCTTCAGGAGCTCTGGATCACT GTTCAACAACGCCATGAAGGGGGGAGTTGATGATGAAATCACCCTCTCTGCCTATATAACTGTGGCCCTT TTGGAAAGTTTACCCCCAGACACTCATCCTGTTGTCTCCAAAGCCCTGAGCTGCCTAGAGTCATCTTGGG AGAACATAGAGCAAGGAGGAAATGGCAGCTTCGTGTACACCAAGGCACTGATGGCCTATGCTTTTGCTCT GGCGGGGAATCAGGAGAAGAGAAACGAAATCCTGAAATCCCTTGATAAGGAAGCTATAAAGGAAGACAAC TCCATTCACTGGGAAAGACCTCAGAAACCCATGAAATCAGCGCGTTCTCTGTACAAACCCCAAGCTTCCT CTGCTGAAGTAGAGATGAATGCCTATGTCATCTTAGCTCGCCTCACTGCCCAGTCAGCCCCATCCCCTAA GGACCTGGCTTTGTCAATGGGCACCATCAAGTGGCTCACAAAGCAGCAGAATTCCCGTGGTGGCTTCTCC TCCACACAGGACACTGTGGTGGCCCTCGATGCTCTGTCCAAATACGGAGCAGCTACTTTTTCCAAAAGCC AAAAAACTCCTTCGGTGACCGTCCAATCTTCAGGGTCATTTTTCCAAAAGTTCCAAGTGGACAACAGTAA TCGTCTGTTACTGCAGCAGGTCTCATTACCAGACATTCCTGGGAACTACACCGTCAGTGTGTCAGGAGAA GGATGTGTGTATGCTCAGACCATGCTGAGATACAACCTGCCCTTGGAGAAGCAGCAGCCTGCGTTTGCTC TAAAGGTGCAGACAGTACCCCTGACTTGTAACAACCCCAAAGGCCAGAACAGCTTCCAAATCTCACTAGA AATCAGTTACACGGGGAGCCGTCCAGCCTCCAACATGGTGATTGCTGATGTGAAGATGCTCTCTGGTTTC ATCCCATTGAAACCAACAGTGAAGAAGCTTGAAAGATTAGAGCATGTAAGCAGAACAGAAGTGACCACCA ACAACGTCCTGTTATATTTGGACCAGGTAACCAATCAGACACTGTCCTTCTCCTTCATCATTCAACAAGA CATCCCAGTAAAGAACCTGCAGCCTGCCATTGTGAAAGTGTATGACTACTATGAGACAGATGAAGTGGCT TTTGCTGAATACAGCAGCCCCTGCAGCTCAGACAACCAAAATGTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271376
Insert Size	4458 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271376.1, NP_001258305.1
RefSeq Size	4586 bp
RefSeq ORF	4458 bp
Locus ID	297572

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