Adgrl2 (NM_001190475) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Cirl-2; Cirl2; Cl2ac; Lphn2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217639 representing NM_001190475 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGTCTTCTGGTTGCAGAATGCGAAGTCTCTGGTTTATCATGATAATCAGTTTCTCACCGAATACCG AAGGTTTCAGCAGAGCAGCCTTGCCATTCGGGTTAGTTAGACGAGAGCTGTCCTGTGAAGGTTATTCTAT AGACCTGCGATGTCCGGGCAGTGACGTCATCATGATCGAGAGCGCAAACTACGGTCGGACGGACGACAAG ATCTGCGACGCAGACCCCTTTCAGATGGAGAACACAGACTGCTACCTCCCTGATGCCTTCAAAATCATGA CTCAAAGGTGCAACAACCGAACACAGTGTGTAGTAGTTACCGGGTCAGATGTATTTCCTGATCCATGTCC TGGAACTTACAAATACCTTGAAGTTCAATATGAATGTGTCCCTTACATGGAGCAAAAAGTTTTTGTGTGT CCTGGAACCTTGAAAGCAATTGTGGACTCTCCAAGTATCTATGAAGCTGAGCAAAAGGCAGGTGCTTGGT GCAAGGACCCCCTTCAGGCTGCAGATAAAATTTATTTTATGCCCTGGACTCCCTACCGCACCGATACCTT AATAGAATATGCTTCTTTAGAAGATTTTCAAAACAGCCGCCAGACAACAACATACAAACTTCCAAACCGA GTGGACGGTACTGGATTTGTGGTGTATGACGGGGCAGTCTTCTTCAACAAAGAAAGAACGAGAAACATTG TTAAATTTGACTTGAGGACTAGAATCAAGAGTGGGGAGGCCATAATCAACTACGCCAACTACCATGACAC TTCACCCTACAGATGGGGGGGGAAGACTGACATTGACCTGGCAGTGGATGAAAATGGCTTGTGGGTCATC TACGCCACCGAGCAGAACAACGGAATGATCGTGATTAGCCAGCTCAATCCGTACACTCTCCGATTCGAAG CAACCTGGGAGACGACGTATGACAAGCGTGCGGCGTCCAATGCTTTCATGATATGCGGGGTCCTCTACGT GGTCAGGTCAGTGTACCAAGACAATGAAAGCGAAGCTGGCAAGAACGTCATCGACTACATTTACAACACA AGGTTGAGCCGGGGAGAGCACGTGGACGTTCCCTTCCCCAACCAGTACCAGTACATCGCTGCAGTGGATT ACAACCCAAGAGACAACCAACTCTACGTATGGAACAATAACTTTATCTTACGGTATTCTCTGGAGTTTGG TCCACCCGACCCTGCCCAAGTGCCTACCACAGCTGTGACAATAACTTCTTCAGCTGAGCTGTTCAAAACC ACAGTGTCAACCACAAGCAGTACTTCACAGAGAGGCCCCGTGAGCAGCACAGTCGCTGGTCCTCAGGAAG GAAGCCGAGGGACAAAGCCACCTCCAGCAGTCTCTACAACCAAAATTCCTCCTGTAACAAATATTTTTCC CCTGCCAGAGAGATTCTGCGAAGCGTTAGAAATGAAGGGGATAAAGTGGCCTCAGACACAAAGGGGGATG ATGGTTGAGCGACCGTGTCCCAAGGGAACAAGAGGAACGGCCTCGTATCTCTGCATGGCTTCCACAGGAA CCTGGAACCCGAAGGGCCCGGATCTTAGCAACTGCACCTCTCACTGGGTGAATCAGCTGGCCCAGAAGAT CAGAAGTGGAGAGAATGCTGCAAGTCTGGCCAACGAACTGGCTAAGCACACCAAGGGGACGGTGTTCGCT GGGGATGTGAGCTCCTCTGTGAGGCTGATGGAACAGTTGGTGGACATCCTGGATGCCCAGCTGCAGGAGC TGAAACCGAGCGAGAAGGACTCGGCCGGGAGGAGTTATAACAAGCTCCAAAAACGAGAGAAGACATGCAG GGCTTACCTTAAGGCCATTGTGGACACAGTAGATAACCTTCTGAGAGCCGAGACTTTGGACTGCTGGAAA CACATGAATTCCTCAGAGCAGGCGCACACAGCCACCATGCTGTTGGACACCTTGGAAGAAGGAGCATTTG TCCTGGCAGACAACCTTTTGGAACCAACCCGGGTCTCAATGCCAACGGATAATATTGTTCTAGAAGTCGC TGTCCTCAGCACGGAAGGACAGGTCCAAGACTTCACCTTCCATCTCGGCTTCAAGGGGGCCTTCAGCTCC ATCCAGCTCTCAGCCAACACCGTCAAGCAAAACAGCAGAAACGGGCTGGCAAAGGTGGTATTCATCATTT ACCGGAGTCTGGGACCATTCCTGAGCACCGAAAATGCGACCGTCAAACTGGGCGCAGACCTCCTGGGTCG GAACAGCACCATCGCAGTGAACTCGCACGTCCTTTCAGTCTCCATCAATAAGGAGTCCAGCCGTGTGTAC TTGACAGACCCGGTGCTTTTTTCAATGCCACACATTGATTCTGACAATTATTTCAACGCAAACTGCTCCT TCTGGAACTACTCAGAGAGAACCATGATGGGATATTGGTCTACCCAGGGCTGCAAGCTGGTTGACACTAA TAAAACTCGCACGACGTGTGCATGCAGCCACCTAACCAATTTTGCTATTCTCATGGCCCACAGGGAAATT GTGTACAAAGATGGCGTCCACAAATTGCTGCTGACAGTCATCACCTGGGTGGGCATCGTTGTCTCCCTCG TCTGCCTGGCTATCTGCATCTTCACCTTCTGCTTCTTCCGAGGCCTGCAAAGCGACCGCAACACGATCCA CAAGAACCTGTGTATCAACCTCTTCATCGCTGAGTTTATTTTCCTAATAGGCATTGATAAAACACAGTAC ACGATTGCGTGCCCCGTGTTTGCAGGACTCCTGCACTTTTTCTTCCTGGCTGCTTTTTCCTGGATGTGCC TAGAAGGTGTGCAGCTCTACCTCATGTTGGTTGAAGTTTTCGAGAGTGAATACTCAAGGAAGAAGTATTA CTATGTCGCCGGGTACCTCTTCCCTGCCACAGTGGTCGGTGTTTCAGCTGCTATCGACTACAAGAGTTAC GGGACACTAGAGGCTTGCTGGCTTCACGTTGATAACTATTTCATATGGAGTTTCATTGGGCCTGTTACTT TCATCATTCTGCTAAATATTATTTTCCTGGTGATCACGCTGTGCAAAATGGTGAAACATTCAAACACTTT GAAACCAGATTCTAGCAGGTTGGAAAACATTAATAATTACCGTGTTTGTGATGGATACTATAATACGGAC TTACCTGGGTCTTGGGTGCTCGGTGCGTTCGCCCTGCTGTGTCTCCTGGGCCTAACCTGGTCCTTTGGGT TGCTTTTTGTTAACGAGGAGACCGTTGTCATGGCTTATCTCTTCACCGCCTTTAATGCTTTCCAGGGACT GTTTATTTTCATCTTCCACTGTGCTCTTCAAAAGAAAGTACGGAAAGAGTATGCCAAGTGCTTCAGACAC TGGTACTGCTGTGGTGGCCTCCCGACCGAGAGCCCGCACAGCTCTGTAAAGGCGTCCACCTCCCGCACCA GTGCTCGCTACTCCTCTGGTACACAGAGCCGTATAAGAAGGATGTGGAATGACACCGTGAGGAAGCAGTC TGAATCGTCTTTTATCTCAGGTGACATCAATAGCACTTCTACCCTTAATCAAGGAATGACTGGCAATTAC CTACTAACAAACCCTCTTCTTCGACCCCACGGCACTAACAACCCCTATAACACATTGCTCGCTGAAACAG TTGTATGTAATGCCCCTTCAGCGCCCGTGTTTAACTCACCAGGACATTCACTGAACAATACCCGGGACAC CAGCGCCATGGATACTCTACCGCTAAATGGTAACTTCAACAACAGCTACTCCCTGCGCAAGGCCGACTAC CACGACGGCGTGCAGGTTGTGGACTGTGGACTAAGTCTGAACGACACCGCGTTTGAGAAAATGATCATTT CAGAGTTAGTGCACAACAACCTCCGGGGTAGCAACAAAACCCACAACTTGGAGCTCAAGCTCCCGGTTAA ACCCGTGATTGGCGGCAGCAGCAGCGAAGATGACGCGATCGTGGCCGACGCCTCATCTTTGATGCACGGT GATAACCCAGGGCTGGAATTCCGCCACAAAGAGCTGGAGGCACCGCTCATCCCTCAGCGGACTCACTCGC TTCTGTACCAACCCCAGAAAAAAGTGAAACCCGAGGCAACCGACAGCTACGTCTCCCAGCTGACGGCCGA GGCCGACGAGCACCTCCAGTCCCCCAACAGAGACTCTCTGTACACGAGCATGCCCAACCTAAGAGACTCT CCCTACCCGGAGAGCAGCCCGGACATGGCAGAGGACCTGTCTCCCTCCAGGAGGAGCGAGAACGAGGACA TTTACTACAAAAGTATGCCCAATCTTGGGGCTGGCCGCCAGCTCCAGATGTGCTACCAGATCAGCAGAGG CAATAGCGATGGCTACATCATCCCCATTAACAAAGAAGGGTGCATCCCAGAGGGGGACGTCAGGGAAGGA CAGATGCAGCTGGTAACAAGTCTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001190475
Insert Size	4437 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001190475.2, NP_001177404.2
RefSeq Size	6255 bp
RefSeq ORF	4437 bp
Locus ID	171447
UniProt ID	O88923
Gene Summary	low affinity G-protein-coupled receptor for alpha-latrotoxin [RGD, Feb 2006] Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region, compared to variant 1. The resulting isoform (2) is shorter, compared to isoform 1.

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