Pola1 (NM_053479) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217636 representing NM_053479 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCCTGTTCACGGAGACGACTGCAAACTGGAAACAAGCGCTGTGTCAGATTCAGGGAGTTTTGTAG CTTCTCGAGCACGTCGAGAAAAAAAATCAAAGAAAGGACGCCAAGAAGCTTTAGAGCGACTGAAAAAGGC TAAAGCTGGTGAGAAGTACAAGTATGAAGTTGAGGACCTCACAAGTGTTTATGAAGAAGTTGATGAAGAG CAGTATTCAAAACTGGTTCAGGCACGTCAGGATGATGACTGGATCGTGGATGACGACGGTATAGGCTATG TGGAAGACGGTAGAGAGATTTTTGATGACGACCTTGAAGATGATGCACTTGACACCTGTGGGGAAGGAAG TGATGGCAAAGCACACAGAAAGGACAGGAAGGATGTAAAGAAACCTTCAGTGACAAAACCAAACAATATC AAGGCAATGTTCATTGCTAGTGCTGGGAAGAAAACCACAGATAAAACAGTTGACTTGTCCAAGGATGATC TGCTAGGTGACATTCTACAGGATCTGAACACTGAGACACCTCAAATAGCTCCACCGCCAGTATTGATACC AAAGAAAAAAAGATCTACTGGAGCTTCACCGAATCCTTTCTCTGTGCACACCGCCACGGCAGTTCCTTCA GGAAAAATTGCTTCCCCTGTCTCCAGAAAGGAGCCTCCATTAACTCCTGTTCCTCTTAAACGTGCTGAAT TTGCTGGCGATCTGGCTCAGCCAGAGTGTCCAGAAGATGAGCAGGAGTCAGGGGTTATAGAGTTTGAAGA TGGCGACTTTGATGAGCCCATGGACACAGAGGAAGTAGATGAGGAGGAGCCTGTGACTGCCAAGATTTGG GACCAAGAGAGTGAGCCAGTGGAAGGGGTGAAGCATGAGGCAGATCCTGAGACGGGAACCACATCTTTCT TAGACAGTTTTCTCCCAGATGTTTCTTGTTGGGACATTGATCAGAAGGACGAAAACAGTTTCTTACTTCA AGAAGTTCAAGTGGACTCCAATCATCTCCCGTTGGTAAAAGGGGCAGATGATGAACAAGTCTTTCAGTTT TATTGGCTGGATGCTTATGAAGATCCATACAACCAACCAGGTGTGGTTTTTCTGTTTGGCAAAGTATGGG TCGAATCAGCCAAAACCCATGTGAGCTGTTGTGTTATGGTGAAAAATATTGAGCGAACACTCTACTTCCT TCCCCGTGAAATGAAAATTGACCTAAACACAGGGAAAGAAACAGCAACTCCAATTACAATGAAGGATGTT TATGAAGAATTTGATTCAAAAATTTCAGCAAAATATAAAATAATGAAGTTCAAGTCCAAGATAGTGGAAA AGAACTATGCTTTTGAGATACCTGATGTTCCAGAAAAATCTGAGTACTTGGAAGTTAGATACTCGGCAGA AGTGCCACAGCTTCCACAGAATTTAAAAGGAGAAACATTTTCTCATGTATTTGGAACTAACACGTCTAGC TTGGAACTGTTCTTGATGAATAGAAAGATCAAAGGACCCTGTTGGCTTGAAGTAAAAAATCCACAACTCT TGAATCAACCAATCAGTTGGTGTAAATTTGAGGCAATGGCTTTGAAACCAGACTTGGTGAATGTAATTAA GGATGTCAGTCCTCCTCCACTTGTGGTGATGTCTTTCAGCATGAAGACAATGCAAAATGTACAGAACCAT CAACACGAGATCATTGCTATGGCAGCGCTGGTTCATCACAATTTCCCATTAGATAAAGCACCTCCAAAAC CTCCCTTTCAGACGCATTTCTGTGTTGTGTCTAAACCAAAGGACTGTATTTTCCCATGCGCTTTCAAAGA AGTCATTAAGAAAAAGAATATGGAGGTTGAGGTTGCTGCAACAGAAAGAACTTTGCTAGGATTTTTCCTT GCAAAAGTTCACAAACTTGACCCTGATATTCTCGTGGGTCATAATATTTGTGGTTTTGAACTAGAAGTGC TACTGCAAAGAATTAATGAGTGCAAGGTTCCTTTCTGGTCCAAGATAGGTCGGCTGAGGAGATCCAACAT GCCAAAACTTGGGAGCCGGAGTGGATTTGGTGAAAGAAATGCTACCTGTGGTCGAATGATATGTGATGTG GAAATTTCAGTAAAAGAATTGATTCATTGTAAAAGTTACCATTTGTCTGAACTTGTTCAGCAGATTCTGA AAACTGAGAGGATTGTAATTCCAACAGAAAATATACGAAACATGTATAGTGAACCTTCTCATCTGTTGTA CCTGTTGGAACACATCTGGAAAGATGCCAGATTCATTTTGCAGATCATGTGTGAGCTAAATGTTCTTCCA TTAGCATTGCAGATCACTAACATCGCTGGGAACATTATGTCTAGGACACTGATGGGTGGACGATCTGAGC GAAACGAGTTTTTGTTGCTTCATGCATTTTATGAAAACAACTATATTGTGCCTGACAAGCAGATTTTCAG AAAACCTCAGCAAAAACCGGGAGATGAAGATGAAGAAATTGATGGGGATACCAATAAATATAAGAAAGGA CGCAAGAAAGCAGCTTATGCTGGAGGCTTGGTTTTGGACCCCAAAGTTGGTTTTTATGACAAGTTCATTT TGCTCCTGGATTTCAACAGTTTATATCCTTCCATCATTCAGGAATTTAACATTTGTTTTACAACAGTGCA AAGAGTTGCTTCAGAGACACTGAAAGCTACAGAGGATGAGGAACAAGAACAGATTCCAGAGTTGCCAGAT CCAAACTTAGATATGGGCATTTTGCCCAGAGAGATCCGGAAACTGGTAGAGCGGAGAAAACAAGTGAAAC AGCTAATGAAACAGCAAGATTTAAATCCAGACCTTGTTCTTCAGTATGATATCCGACAGAAGGCTTTGAA GCTCACAGCGAACAGTATGTATGGTTGCCTGGGATTTTCGTACAGCAGATTTTATGCTAAACCGCTGGCT GCCTTGGTGACATACAAAGGAAGGGAGATTTTGATGCACACGAAAGAGATGGTACAGAAGATGAATCTTG AAGTTATTTATGGAGACACAGATTCAATTATGATAAATACCAATAGCACCAACCTGGAAGAAGTCTTTAA GTTGGGAAACAAGGTAAAAAATGAAGTGAATAAATTGTACAAACTGCTTGAAATAGACATTGATGGTGTT TTCAAGTCTCTGCTATTGCTGAAGAAAAAGAAATATGCTGCTCTAGTTGTCGAACCAACATCCGATGGGA ATTACATCACCAAGCAGGAACTGAAAGGATTGGATATAGTTAGAAGGGACTGGTGTGATCTTGCCAAGGA CACCGGAAACTTTGTCATTGGCCAGATTCTTTCTGATCAAAGTCGGGACACCATTGTGGAAAACATTCAG AAGAGGTTAATAGAAATTGGAGAAAATGTGCTAAACGGCAGTGTCCCAGTGAGCCAGTTTGAAATTAACA AGGCATTGACAAAGGATCCCCAGGATTACCCTGATAAAAAAAGTCTACCTCATGTACATGTTGCACTGTG GATAAATTCTCAAGGAGGCAGGAAGGTGAAAGCTGGCGATACAGTGTCATATGTTATCTGTCAGGATGGG TCAAACCTCCCTGCAACTCAGAGGGCCTATGCACCCGAGCAATTGCAGAAACAGGACAATTTAGCCATCG ACACCCAGTACTACTTGGCCCAGCAGATCCACCCTGTTGTGGCTCGAATCTGTGAGCCAATAGATGGAAT TGATGCTGTACTCATTGCATTATGGCTAGGACTTGACTCCACTCAATTTAGAGTTCATCAGTATCACAAA GATGAAGAGAATGATGCTCTCCTTGGTGGTCCAGCACAGCTAACTGATGAAGAGAAATACAAGGATTGTG AAAAATTCAAATGCCTTTGTCCTTCATGTGGAACTGAAAATATTTATGATAATGTCTTTGAAGGCTCGGG AATGGATATGGAGCCCAGCTTGAATCGTTGCAGTAATATTGATTGTAAGGCTTCACCAGCGACCTTTATG GTACAGTTGAGCAACAAATTGATCATGGACATTAGACGTTGCATTAAAAAGTACTATGATGGCTGGTTGA TATGTGAGGAGCCAACTTGTCGCAATCGCATTCGTCGTCTTCCCCTTCATTTCTCCCGAAATGGGCCTCT GTGTCCAGCCTGCATGAAAGCTGTGCTCAGACCAGAGTATTCTGACAAGTCCCTGTACACCCAGCTGTGC TTTTACCGGTACATTTTTGATGCGGACTGTGCACTGGAAAAACTTCCTGAACACGAGAAAGATAAATTGA AGAAGCAGTTTTTCACTCCCAGAGTTCTTCAGGACTACAGAAAAGTCAAGAACATAGCAGAACACTTCTT GTCCTGGAGTGGCTACTCTGAAGTAAACCTCAGCAAGCTCTTCGCCAATTATGCTGGGAAGAACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053479
Insert Size	4407 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053479.1, NP_445931.1
RefSeq Size	5321 bp
RefSeq ORF	4407 bp
Locus ID	85241
UniProt ID	O89042
Gene Summary	exhibits DNA polymerase alpha activity; may mediate DNA replication process [RGD, Feb 2006]

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