Hfm1 (NM_001191101) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217622 representing NM_001191101 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGAATCAAACGATTGCATTTTATCCATGGATTCTTTGTTTTTCAGTAGCCCAGGTGAAACTGAAA GCTTTCCAGTTGAGGAAAAATCACTGGATTGGTTTCTCCCTCCAGCTCCACTGATCTCAGAAATTCCAGA TATTCAAGAGTTAGAGGAAGAAATTGAAAATTATAAGCTATTAGGAGAAAGAAAGATGCCCAATATATTA ACATCAAACTTGAAGATCATTAATGAAGACACAAATTGTATTTCACCAACACAGAAATTCCATTTTCCCT ATAACATACATGAACAGGATTACCTAAATTTAGGAGGATTAAATAACAATGACATGTCACATACAGCTGG CAAGCTAGTGTACGGTTCTTCTCAAAAATATAAAAACCACATGGGGGATGAGAGTCCACCGACAAAGAGT GGTCCTGGTGATGCAAAGCTGCATACTGCTGCTGAGGACAGAGAGGGCACATCAGCACTCAAAACCAGAT TATCTAGAACATGTGGCAGTGTGCAGGAATGTGACTATGCTAATGGAAATTTAAGCTTGAATTCTCATAT TAGCCCAGCGAGACTCACCCAAACAAAAGTAAGCAAAGAGAAACCATGGAACTGTAGCAATAGAAAACAG AAACTGCAGTATTCTACAGACAAATCCAAAGCAAACGATGCTTTTTCTGCTTCTGGAATTGAAAAAGACA TATTCGAAGCACCATCGTTTTCTGCTGCATCTCAGCCTCGTGACATTCAAGGCATAACCTCAAATGGCTT AGGTTCGTTGAAGGCTGTCACAGAAATCCCTGCAAAATTTAGAAGTGTATTCAAAGAATTCCCTTACTTC AACTATATACAGTCCAAAGCCTTTGATGATCTTCTTTACACAGATCGGAATTTTGTGATTTGTGCCCCCA CTGGTTCTGGGAAAACTGTGGTGTTTGAGCTAGCAATAACAAGATTGTTAATGGAAGCACCATTGCCGTG GCTGAACATGAAAGTTGTTTACATGGCACCAATAAAAGCCCTTTGTAGTCAGCGGTTTGATGACTGGAAA GAAAAATTTGGACCGATAGGCTTGACCTGTAAAGAACTTACTGGAGATACAGTAATGGATGACCTGTTTG AGATTCAGCATGCCAATATAATTATGACAACTCCAGAGAAGTGGGACAGTATGACTAGGAAGTGGAGAGA CAACTCCTTCATCCAACTGGTTCGCCTGTTTCTCATTGATGAGGTGCATGTTATTAAAGATGAAAACCGT GGTCCAACTCTTGAGGTTGTAGTAAGCAGAATGAAAACTGTCCAGTCTTTATCTAAGACCTTAGAAAACG CCAGCCCTGCTCCAGTGAGGTTTGTAGCTGTATCAGCAACAATTCCCAATGCTGAGGATATCGCAGAATG GCTTTCAGATGGGGAGAGACCTGCTGTGTGTCTGAAAATGGATGAGAGCCACAGACCTGTGAAACTTCAG AAAGTGGTCCTGGGTTTCCCCAGTAGTAGTAACCAAACTGAATTCAAGTTTGATTTAGCCCTCAACTATA AAGTGTACAGTGTTATACGAACGTACTCCGATCAGAAGCCCACACTCGTGTTTTGTGCAACGAGGAAAGG TGTGCAGCAGGCTGCTTCTGTTCTTGTGAAGGACGCTAAGTTTATCATCACCGTGGAGCAGAAACTACGG TTGCAGAAGTCTGCATATTCTATAAGGGATTCAAAGCTAAAAGATACCTTGGTGTACGGTGTTGGTTACC ATCATGCTGGAATGGAGCTGTCAGATAGAAAATTGGTTGAAGAGTTGTTTACTTCTGGAGATCTACCAGT TCTTTTTACTACAAGTACTCTAGCTATGGGAGTGAACTTGCCGGCTCACCTGGTCGTTATAAAGTCCACC ATGCACTACACTGGAGGTGCCTTTGAAGAGTACAGCGAGACAGACATCCTGCAGATGATTGGAAGAGCTG GCAGGCCCCAGTTTGACACCACAGCTACTGCAGTTATTATGACCCGACTAAGTACAAGAGAGAAGTACGT TCAGATGCTAGCTTGTAATGACACTGTGGAAAGCAGTTTGCACAGACATCTTATTGAGCACCTGAACGCA GAGATAGTGCTGCACACCATCACAGATGTGAGCATCGCCCTGGACTGGATCCGGTCCACACTGCTGTATA TCAGAGCTCTGAAAAACCCATCTCACTATGGTTTTATATCTGGATTGAACAAAGATGGAATTGAAGCAAA ATTACAAGAGTTATGTTTGAAGAATCTGAAAGATTTATCATCGCTAGACCTAATAAAGATGGATGAAGAC CTTAATTTCAGATCAACAGAGGCAGGAAGACTAATGGCATGGTATTATATTACATTTGAGACAGTGAAGA AATTTTGTGCAATCAGTGGAAAAGAAACTTTATCAGATTTGATTTCAATGATATCCAGCTGCAGTGAGTT TCTAGATGTGCAGCTGAGGATAAGCGAAAAGAGAACACTGAACACACTAAACAAAGACCCAAACCGGATA ACCATCAGATTTCCAATGGAGGGGAGAATTAAAACCAGAGAGATGAAAGTAAACTGTCTTATTCAGGCTC AGCTAGGATGCATCCCTATACAAGATTTTGCTTTGACACAAGATACCTCGAAGATTTTCAGAAATGGCTC ACGAATTGCAAAATGGTTGTCAGATTTTGTGACTGCTCAGGAACAGAAGTTTGCTGTGCTTTTAAACAGT GTGATTTTAGCTAAATGTTTTAAGTGTAAACTCTGGGAAAACTCTCAACATGTTTCAAAACAGCTGGATA AAATTGGCATATCATTGTCAAATACCATGGTAAATGCAGGCTTGACGTCTTTTAAAAAAATAGAAGAAGC AAATGCGAGAGAACTTGAACTGATTTTAAATAGACACCCGCCATTCGGGACCCAGATAAAGGAAGCTGTG ATGTATCTGCCAAAGTATGAACTTGAGGCAGAGCAGATTGCAAGATATAGTGATACCAAGGCAGAAATAC TGGTGACCATTATATTAAGAAACTTTGAGCAGCTGCAGACCAAAAGAACAGCGCCAGACTTTCACTATGC CACCTTAATCATAGGTGATGCAGATAACCAAGTCGTTTGTAAGCACAAAATTATGGATTCTGTCTTGTTA AAAAGTGGAAATTGGGTTAAAAAAATTGATGTGAAGAGAGCTCTTATATCAGAAGATCTCAGCATAAATC TAATTAGCTCTGATTATGTTGGTCTTGATATTCACCAGAAATTTACAGTCTTTTATTTAGGACCCAGGAA GTTTGTAAATCAAACAGTTACGGAAAGAAGCTCAGGAACAGGTCTTTCTCATTCCGAATGCTCAGACAGA GCTACTGCAGCAGGATCCAGTAAAAGAACGGCTACCTGCAGAAACCCTGGGAACCGAGAATGCCATCATC ACTGTAAAAATAAGCACATATGTGGGCATGACTGCTGTAAAATTGGAGTTGCACAAAAGCCAGAAGCTAA AGTGTCAGCAGTGTCCTCATATCTGTCTGACCTAAAAAGCAGAGATGCTGTTTCTTCTTTTCCCCCAGCT AAGCGACTCAAGATACAGATGAACAAATCTCAAAATGTGGACCTTAAAGAATTTGGTTTTGTTCCAAGGC CTTCCCTTTCCAGTATAGCAAGGTCAGAATATTTAAACATCCATAAATTGCCAATATTAGAACAGAGGAC TCAGCATGAGATCTATGGCGAAGTTGAAGAGGGAGCGTCTGAATGCCGAGACGAAGACGGCCCACATGTG AACTCAGAGCTGGGAGATGAAGTTTGGGATGACTTTGATGATGAAAGCTTAATAGAAGTTATGAGCTTTT CAGCTGACGCCGAGGAGATGGCTGTGTCAGGGTTTGGAGACACTCGTGATTCAAGTCTAGGCAGAAGTAA GCTACACTTCGGAAGATCAAGCAGCAGGTTCCAAAGAGACATCTCAAACAGTTTTGTTTCATCACCCGAG AAGCCAGATGTTTGTTTGTCAGATTGTTCCGTGTCTAATTTCGGGCTGTCCTCTGTGGCAGAGTTCCCTC AACAAGCTGGAAATGCAAGTCTTGTCAATTTACAAGAGAGAAAACAGCTGACCCTCTCACCAGTGATTGA GAGGATGTGCTTTACCCACTCTGAGAAAATACCACAGTCTCCCAGTTTTAAGGAAGTGGATATTTTTATT GGAAACAGTGACTCTAAAAAGGAAATTGATCTTAGTAAGTATTATCCTGATGATGCAGCCGAGGAAATGA AGGCCCTTCTGGGAATATTTAATGGCATTTTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191101
Insert Size	4305 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191101.1, NP_001178030.1
RefSeq Size	4305 bp
RefSeq ORF	4305 bp
Locus ID	690161

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