Cep162 (NM_001277060) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Qn1; RGD1307365
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217612 representing NM_001277060 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCACTATTCCAAAGTAGACCTTGATGAAGAATTTGAACGGTTTATGAAGGAGCTTTCAGATGATT CTTTTGAAAATTCAAATGAAACACCTAGCCAGCCTAACAAAGACAGAAAGAAGAAAGACACGGCCCCTTG GTGGATAGCTGAAGATGGTTTTGAAGATGATGGACTGCTTGGGACAAATGTGAGCTATTTGAAGACAAAG AAGACCTATCAACCTATTACGGACATGGAAGAGGAAAATGAAAAAGTCCAGTTTCTTAAGAGCAGTGGAA CCTCCGTCTTAAGTGTTGACAGCTTAGAAGCTAATGAATTGGTTGCTTCTGAGCTCCATCATAGCACTCT AGGTTTGGGCCTGGACACATTAGAAGAACAAGAGGAGAAAGAGCAGTTCTTTGCCAGGCTTGAGAAAGGC TTGACATCTTCTATTGATTATTCAAAATTAAATCAAGAACTGGATTCTGACGACTCTGCACAATTCAGAG TTTTACATAGATATCAAGGTAATGTAGAACCAGCTGAAGGTGGACGTGAGAATGAATCAGAACACAAGGA ACTGCCAGAAACATACAGTGATGATTTTGAAGATGCTGAGGATACTGATGAACCTTTGATTACTAAAGAT GAAGAGACCCGTCCCAAAGAAAACCCTGAGTCAGGAAAAGGCAGCTTTCCCAACCAGGAGGAAGAGAAAA CTGGCATGCTGGCTAATGTGGTGCTGCTTGATTCTTTTGACTCTGTCGAGGATGTTGACCTTAACAATCA TGAGAGACCAACCCCTAAGGCAAAGGCCCTCCCAGAAATGGCAGGCGGTGAACTAGCAGAAACAGGTGTT TCTTATGGACAAAGCAGTGGTGACACAGAAGCCCTGCACCAGGCTTACCATCACGTAGCTCATTCTCTGG GAGATACAGGCGAACCAAGAATTGAGGCTAGCCCTGGGCACAGTGTCAGGAGCTCCGCAAAAGATGGTCT TCAAGAAAATGAGGAGTCTTCAAAGAACATCTCTACCACCGAGTCTGATCTTCCCACAGTAGAGGAGCTG ATGAAGCCTATCAGGATAGATTCCTATGGGATCAGGGGTTTTGACTTGCAGCCTGTCAGCCTTCAGAAAG CAGTGGGCAGTAAAGAAGCAGAGTCTGTGAGCTCTTTACCCCTGACAGTGAACACAAACACCATGTCTCA AGACACTAGGCACGTGAGCCCATTTCCCCACGAGCAGGATGAGAGTGTGGTTCTACACAGGACAGCGGGG GAGGGTGTGGGCAGCAGCTGCCCAGCCACAGAAGAGCATTTGGACAAAATGTACCTGGAGATTCTGAGGA AGAAAACATCTGTTAATCCTTCACTGTTACCTCAGGACAACAAAGCTAACCAGACTTCCAGGTCCCGGCT CAGTGCTAGGGAAGAGGCGCCTGTCACTAGTAAACAGGTTCCATGTAAGAAGGCCAGAAGTGCACCTCCT TTGCCTAGGAGAAAGCCACAGAGTGGGCTGTATGCATCAGCTCGGAGCTCAGGCTACAGCAAACCCAGTT CACCACTCCAGTTCTTTTCAGCTCTTGAAAAGAAAACGTCCAAGGATAATACAAAAACTAAAAATGTGAG ACCCATTCCCACCTCAAATCAATTTAGAAAAAGAGAAATACTATCTGGAACAAAGCTCATCAAGCCTGCA GCTTTGAACAAACCATCCCCTCACCGTGAAGGCAGCCCAGCTACACCTAAGAGGCCTGAAGACCCCTCAG ATGATTCCTTTGTTCAGCTACAGACTGAGACTTTGGGATCCTATGGTGGGAACAGAGAAAAAGAATTACT AATGTTGAAAAGAGCTCAGGACGCTGAGGAAAAATGGAGGGGCGCCCAAGCGCTAATCGAGCAAATTAAA ATGACGTTCTCTGAGAAGGAGAAGGAGTTGGAGAACACAGTGGAAAGCCTAAAGAGGCAGCAGGAGAGAG AGCTCTTCAAACTGAACCAAGAAAATTACATTCTTCAAGCCAAGTTAAGTAGCTTTGAAGAAACAAGCAG AAAACAGAGGTGGCTACAATTTGGAGAAACATCTGATCCTCTCACTGAAGAGAAACTGAAACAAATTCAA AAAGAGATACAAGAACAAGAGACACTTCTTCAAGGCTACCAACAGGAAAATGAAAGGTTATATAGTCAAG TAAAAGATCTTCAGGAACAAAATAAGAAAAATGAAGAGCGGATGTTTAAAGAAAATCAAAATTTATTTAG TGAGTTGGCCTCTTTAAAAGAACAGATGCACAAAAATCATTTTCTGCCTCAAGCAGTTGAAAATATAGAA CCTACCAAAAACCAGAGTTTTACAGATCTTCTAGCAGAATTACGGGCAGCACAGAAAGAAAAAAACCATC TGATGGAAGACATCAAAAGACTGAAGCAAGACAAACAAGCTCTTGAAGTGGACTTGGAAAGAGTGAAGAG GGAGAGGGACCAAGCCAAAGATCAAATCGCCTATACCACAGGTGAAAAATTATATGAAATAAAGATTTTA GAAGAAACACATAAGCAGGAAGTCAGTCGTCTACAAAAGCGGTTACAGTGGTACGCTGAAAATCAGGAGC TTCTGGATAAAGACGCAGCCCGACTCAGAGAGGCCAATGAAGAAACTGAGAGGCTGAAACTAGAGATTGA GAAACTGAAAACTGAGTCTGGGAGCCCAGCAAATCAGCAGAGGCTACGCTCAAAGGAGAGAGCTCTTGAT GCCAAAAGAATTCAGGACCTGGAGCGGCAGGTTAAAGAAATGGAAGGGATTCTGAAGAGAAGGTATCCCA ACTCTTTACCCGCTTTGATACTGGCTGCCTCGGCAGCTGGTGATTCAGTAGATAGAAATACGGTGGACTT TATGGAGAGAAGGATAAAAAAGCTAGAAGCTGATCTAGAGGGCAAAGATGAAGAAGCCAAGAAAAGCCTT CGCACCATGGAACAGCAGTTTCAGAAAATGAAGATTCAGTATGAACAGCGGCTGGAAGAGCAGGAGCAGA TGCTTGCCCACAGGCAGAGGGAAGCTCCACAGAACCAGCATAACAGCTCATCCAGGCTCAAGGCACTCGA GACAGAACTTGGGGGCATAAAGGAAGCCCACCAGATCACTGTAAGAAAGCTGGAGGCTGAGATAGATGTT CTTAAACATCAGAATGCTCACTTAGAGCACAAGAAGAATGACAAGGAAGATCAGGACCTCCAGTCCATAG AGTTCCAGGTAGAGCAGGCGCAGGCTAGGGCAAAGCTGGCCAGACTCAATGAAGAACTGGCGGCGAAAGG GAGAGAGATACAAGACCTTACAAAAACTGTGGAGAGGCTCCAGAAGGAGAGAAGGATGATGCTGTCTAGG CAGAGTCCCAGAGGCAGAGAAGAGATGGCTGCCAAAAGGCTGAAGAAAGAAATTTTGCATCCAAATAGTG GAAATGCAAATGCCTTCTCTGAAACATTGGGTGCCAAACTGTATCACCCACACACCTTCACTGACTCTCA CATTTCGGAGGTCTTAGAAGAAAACTACAGATTGAGAAGTGAGCTGGAGGGGTTGACTTTAGAGAGAGAG AAGCTGAAGATGGAATCTGAAGCTGCCGTGTGCCAACTGGAAAGCTCCATGAAAAGGGTCAAGGACGATG CAGCAGCACACATTGCATCCCTCAAAGCAGCTCATGAGAGGGAAATAGAGAAACTCCTTTGCCAAAATGC AGTAGAAAATTCCTCTTCCAGAGTAGCTGAACTGAATCGCAAAATCGCAACTCAAGAGGTTCTTCTAAAA CATTTCCAAGGTCAAGTTAATGAGCTGCAGGGCAAACAGGAATCCCTTTCACTTTCTCAAGTTCGAGAAG AAATCCTACAGAAACAGATTACAAAACTCTTGGAAGAATTGAAAGAAGCCAAAGAAAACCACACACCAGA GATGAAACATTTCATGGGCTTAGAAAGGAAGATCAAGCAGATGGAGATGAGGCATAAGCAGAGAGAGCAG GAGCTCCAGCAGATAATACAGCAAACACGCCAAGTAGTAGAAACTGAGCAAAACAAAGAAGTTGAGAAAT GGAAAAGACTTGCACAGTTAAAGAATCGGGAGCTGGACAAGTTCCGAACAGAGTTAGACTCCATACTCGA CGTTCTTCGGGAGCTGCACAGACAGGGGGTCGTTGTCCCGGTTGCTCTTGCAGATGAAGAGAGCACAGCA AAAGAGTTTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001277060
Insert Size	4212 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001277060.1, NP_001263989.1
RefSeq Size	5441 bp
RefSeq ORF	4212 bp
Locus ID	300880
UniProt ID	Q4KLH6
Gene Summary	Required to promote assembly of the transition zone in primary cilia. Acts by specifically recognizing and binding the axonemal microtubule. Localizes to the distal ends of centrioles before ciliogenesis and directly binds to axonemal microtubule, thereby promoting and restricting transition zone formation specifically at the cilia base. Required to mediate CEP290 association with microtubules (By similarity).[UniProtKB/Swiss-Prot Function]

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