Mink1 (NM_001271136) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	MEKKK 6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217598 representing NM_001271136 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGACCCAGCCCCCGCCCGAAGCTTGGACGACATCGACCTGTCTGCCCTGAGGGACCCTGCAGGAA TCTTTGAGCTGGTGGAGGTGGTTGGCAATGGAACCTATGGACAGGTGTACAAGGGGCGGCACGTCAAGAC TGGGCAGCTGGCTGCCATTAAGGTCATGGATGTCACAGAGGATGAGGAGGAAGAAATCAAACAGGAAATC AACATGTTAAAGAAGTACTCTCACCATCGCAATATTGCCACCTACTACGGGGCCTTCATCAAGAAGAGCC CTCCTGGGAACGATGACCAGCTCTGGCTGGTGATGGAGTTCTGTGGTGCTGGCTCAGTGACCGACCTGGT AAAGAACACGAAAGGAAACGCGCTGAAAGAGGATTGCATTGCGTACATCTGCAGGGAGATTCTCAGGGGT CTTGCCCATCTCCATGCCCACAAGGTGATCCACCGAGATATCAAGGGACAAAATGTGCTGCTGACAGAGA ATGCTGAAGTCAAGCTAGTGGACTTTGGGGTGAGTGCTCAGCTGGACCGCACTGTGGGGAGGCGGAACAC TTTCATTGGGACCCCATACTGGATGGCTCCAGAAGTCATTGCCTGTGACGAGAACCCCGATGCCACCTAT GACTACAGGAGTGACATTTGGTCTCTAGGAATCACAGCCATTGAAATGGCAGAGGGAGCCCCCCCTCTGT GTGACATGCACCCTATGCGGGCTCTCTTCCTCATCCCTCGGAACCCTCCCCCCAGGCTCAAGTCAAAGAA ATGGTCTAAGAAGTTCACTGACTTCATTGACACGTGTCTCATCAAGACTTACCTGAGCCGACCACCAACA GAACAGTTACTCAAATTCCCCTTCATCCGAGACCAGCCCACGGAGCGGCAGGTCCGCATCCAGCTCAAGG ACCACATTGACCGCTCCCGGAAGAAGCGGGGGGAGAAAGAGGAGACAGAGTATGAGTACAGTGGCAGTGA AGAGGAAGACGACAGCCATGGAGAGGAAGGCGAGCCAAGTTCCATCATGAATGTGCCTGGGGAGTCCACA CTCCGCCGAGAATTCCTCAGACTCCAACAGGAGAATAAGAGCAACTCAGAGGCTTTAAAGCAGCAGCAGC AGCTGCAGCAGCAGCAGCAGCGGGACCCTGAGGCACACATCAAACACCTGCTGCACCAGCGGCAGCGTCG CATAGAGGAGCAGAAGGAGGAGCGGCGACGTGTAGAGGAGCAACAGCGGCGAGAGCGAGAACAGCGCAAG CTGCAGGAGAAGGAGCAGCAGCGGCGGTTGGAAGACATGCAAGCCCTTCGTCGAGAGGAAGAGAGGCGGC AAGCAGAGCGGGAACAGGAATACAAGCGGAAGCAGCTGGAGGAGCAGCGGCAGTCGGAGCGGCTCCAGAG ACAGCTGCAGCAGGAGCATGCCTACCTCAAGTCCCTGCAGCAGCAGCAGCAACAGCAGCAGCAGCTCCAG AAACAACAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGATCCTACCTGGAGACAGGA AACCCCTGTATCATTATGGTCGGGGCATTAATCCTGCTGACAAGCCAGCATGGGCCCGAGAGGTGGAAGA GAGAGCTCGGATGAACAAGCAGCAGAACTCTCCCTTGGCCAAGACAAAGCCAAGCAGTGCGGGGCCAGAG CCCCCCATTCCCCAGGCCTCTCCTAGCCCCCCAGGACCTCTTTCCCAAACTCCTCCTATGCAGAGGCCTG TGGAGCCTCAGGAAGGACCACACAAGAGCCTGGTGGCACACCGGGTCCCACTGAAGCCATATGCAGCACC TGTACCCCGATCCCAGTCCCTGCAGGACCAACCCACTCGAAACTTGGCTGCCTTCCCAGCCTCCCACGAC CCTGACCCTGCTGCTGTCCCCACACCCACTGCCACACCCAGTGCCCGAGGAGCTGTCATCCGCCAGAATT CAGACCCCACCTCTGAAGGGCCTGGGCCTAGCCCTAACCCTCCATCCTGGGTCCGGCCTGATAATGAAGC TCCACCCAAGGTTCCTCAGAGGACCTCGTCTATTGCCACTGCCCTTAACACCAGTGGGGCTGGAGGGTCC CGGCCAGCTCAGGCTGTCCGTGCCAGACCTCGCAGCAACTCCGCCTGGCAAATCTATCTGCAGAGGCGGG CAGAGCGAGGCACCCCCAAGCCTCCTGGGCCCCCAGCTCAGCCCCCTGGCCCGCCCAACACCTCTAGTAA CCCTGACCTCAGGAGGAGTGACCCTGGCTGGGAGCGTTCAGACAGTGTCCTCCCTGCCTCCCACGGCCAC CTCCCTCAGGCCGGCTCCTTGGAGCGGAACCGAAACCGTGTGGGAGCCTCCACAAAACTGGACAGCTCCC CAGTGCTCTCCCCTGGGAACAAGGCCAAGCCTGAAGACCACCGCTCAAGGCCAGGCCGGCCCGCAAGCTA TAAGCGAGCGATTGGTGAGGATTTCGTGTTGCTCAAAGAGCGGACTCTGGATGAGGCCCCTAAGCCTCCC AAGAAGGCCATGGACTATTCCTCATCCAGTGAGGAGGTGGAGAGCAGTGAAGATGAGGAGGAGGAAGGCG ATGGGGAGCCATCAGAGGGGAGCAGAGACACTCCCGGGGGCCGTGATGGTGACACAGATAGCGTCAGCAC CATGGTGGTTCATGATGTTGAAGAGGTATCCGGGACCCAGCCTTCATATGGAGGAGGCACCATGGTGGTC CAGCGTACTCCTGAAGAGGAGCGAAGCCTGCTGCTTGCTGATAGCAATGGCTACACAAACCTGCCAGATG TGGTCCAGCCCAGCCACTCACCTACTGAGAACAGCCAAGGTCAAAGCCCTCCAACAAAGGATGGAGGCGG TGATTACCAGTCTCGTGGGCTGGTAAAGGCCCCAGGAAAGAGTTCATTCACCATGTTTGTGGATCTAGGG ATCTACCAGCCTGGAGGCAGTGGGGATACCATCCCCATCACAGCCCTAGTGGGCGGAGAGGGTGGTCGCC TTGATCAACTGCAGTTCGATGTGAGGAAGGGCTCTGTGGTCAACGTCAATCCCACCAACACTCGAGCTCA TAGTGAAACTCCCGAGATTCGCAAGTACAAGAAGCGATTCAATTCCGAGATCCTCTGTGCAGCCCTCTGG GGGGTCAACCTCCTAGTGGGCACAGAGAATGGGCTGATGTTGCTGGACCGAAGTGGGCAGGGCAAAGTGT ATGGACTTATTGGGCGACGACGCTTCCAGCAAATGGATGTTCTGGAAGGGCTCAACCTGCTCATCACCAT CTCAGGGAAAAGGAACAAACTGCGGGTATATTACCTGTCCTGGCTTCGGAATAAGATTCTACACAATGAC CCAGAGGTGGAAAAGAAGCAGGGGTGGACCACCGTGGGGGACATGGAGGGCTGCGGCCACTATCGTGTTG TGAAATATGAACGAATTAAGTTCCTGGTCATTGCTCTGAAGAACTCTGTGGAGGTGTATGCCTGGGCCCC TAAACCCTACCACAAATTCATGGCCTTCAAGTCCTTTGCTGACCTCCCTCACCGCCCTCTGCTGGTGGAC CTGACAGTAGAGGAGGGCCAGCGGCTCAAGGTCATCTATGGCTCCAGTGCTGGCTTCCATGCTGTGGATG TTGACTCTGGGAACAGCTATGACATCTACATCCCTGTACATATCCAGAGCCAAATCACACCCCACGCCAT CGTCTTTCTCCCCAACACTGACGGCATGGAGATGCTGCTTTGCTATGAAGATGAGGGTGTCTACGTCAAC ACTTACGGGCGGATCATCAAGGACGTGGTGCTGCAGTGGGGAGAGATGCCCACCTCTGTCGCCTACATCT GCTCCAACCAGATAATGGGCTGGGGTGAGAAGGCCATAGAGATCCGCTCTGTGGAGACAGGCCACCTAGA TGGGGTCTTCATGCACAAACGAGCCCAGAGGCTCAAGTTCCTGTGTGAGCGGAATGATAAGGTGTTTTTT GCCTCAGTCCGCTCTGGAGGAAGCAGCCAAGTTTACTTTATGACTCTGAACCGTAACTGCATCATGAACT GGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271136
Insert Size	4065 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271136.1, NP_001258065.1
RefSeq Size	5008 bp
RefSeq ORF	4065 bp
Locus ID	303259

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