Wdr19 (NM_001191679) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1306997
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217594 representing NM_001191679 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCGCGTTTTCTCCCTGCTAGAAAAGACTTGGCTTGGTGCTCCAATACAGTTTGCCTGGCAAAAGT CATCAGGAAACTACCTTGCAGTAACAGGAGCTGACTCTGTTGTTAAAATCTTTGATCGCCATGGCCAAAA ACGAAGTGAAATTAGCTTACCTGGCAACTGTGTTGCCATGGATTGGGACAAAGATGGTGACATCCTGGCG GTGATTGCTGAGAAGTCCAGCTGCATCTATCTATGGGACGCCAACACAAATAAAACCAGCCAGCTGGACA ACGGCATGAGGGATCAAATGTCTTTCCTTCTTTGGTCAAAGATTGGAAGTTTCCTGGCTGTGGGGACCAT TAAAGGAAATTTGCTCATTTATAATCATCAGACATCTCGAAAGATTCCTGTTCTTGGAAAACATACTAAG AAAATCACATGTGGATGTTGGAATACGGAGAATCTCCTTGCTTTGGGAGGCGAAGATAGAATGATTACAG TTAGTAACCAGGAAGGCGACACGATAAGACAGACACCAGTGAAATCAGAGCCAAGTGACATAAAGTTCTC CACAAGCAAGACAGATGAGCGGATCTCCTCTGCCGAAAGCACAATAAGTGCGGTAGTTGGCAAGAAAATG TTGTTTCTCTTTCATCTGAATGAACCTGATAACCCGGTTGATCTGGAGTTTCAGCAAGCCTATGGCAACA TCGTCTGCTATAGCTGGTATGGAGATGGCTACATCATGATTGGTTTTTCCCGTGGAACGTTTTTGGCTAT TTCTACTCACTTTCCAGAAGTTGGACAAGAGATATTTAAGACTCGTGACCATAAGGATAATCTAACCAGT GTGGCATTGTCACAGACTCTGAACAAAGCTGCCACATGTGGAGATAACTGCATAAAAATCCATGATCTGA CAGAACTGAGAGACATGTATGCTATAATTAATCTGGATGATGAGAATAAAGGGCTCGGTACCTTATCCTG GACTGACGATGGTCAGTTGCTGGCACTATCTACCCAGAGAGGCTCACTGCATGTCTTCCTGACCAAGCTG CCCATCCTCGGGGACGCCTGTCACACAAGGATTGCATATCTCACCTCCCTCCTTGAGGTCACCGTGGCCA ACCTCATTGAAGGAGAACCGCCAATCACAGTTTCTGTGGATGTTGAACCCAACTTTGTCGCAGTAGGGCT CTATCATCTGGCCGTGGGGATGAACAATCGAGCTTGGTTTTATGTCCTTGGTGAAAATGTTGTCAAAAAG TTAAAAGATGTGGAATATCTGGGAACAGTAGCCAGCATCTGCCTTCATTCTGACTATGCTGCTGCACTGT TTGAAGGCAAAATCCAGTTACATTTGATAGAAAATGAAATGTTGGATGCTCAGGAAGAACGTGAGACCCG GCTCTTTCCAGCAGTGGATGATAAGTGCCGGATTTTATGCCACGCCCTGACTAGTGATTTCCTCATCTAT GGAACTGATACTGGCATTATTCAGTATTTCTTCATCGAAGATTGGCAGTTTGTTAATGATTACCGGCATC CTGTTGGTGTGAAAAAACTATTTCCTGATCCAAATGGAACCAGACTGGTTTTCATTGATGAAAAAAGTGA TGGATTTGTTTACTGTCCTGTGAATGACGCGACCTATGAGATCCCAGACTTCTCGCCAACCATTAAAGGC GTCCTTTGGGAGAACTGGCCGATGGATAAAGGTGTATTCATTGCTTATGATGATGACAAGGTATACACCT ATGTGTTTCACAAGGACACCATCCAAGGATCCAAGGTCATTTTGGCTGGTGGCACTAAACTGCCCTTCTC CCACAAGCCTTTGCTGTTATACAATGGAGAGCTGACCTGCCAGACTCAGAGTGGGAAAATCAACTCCATC TACCTCAGCACCCACAGCTTCCTTGACAGCGTAAAAGACACGGAGCCTCCTGACCTGAGGCAAATGCTGA TGCAGACCCTGATGCTCAAGCGGTTTCCTGATGCTTGGGATATATGCAAAATGCTAAACGACCGCACTTC GTGGAGTGAGCTGGCCAGAGCCTGTCTGCATCACATGGAGGTGGAGTTTGCCATTCGCGTTTCCCGGACG ATGGGGGACGTTGGCACCGTGATGTCCTTGGAACAAATAAAGGGAATTGAGGACTACAATCTTTTGGCAG GACATCTCGCCATGTTTACTAATGACTTCAACCTGGCTCAGGACCTGTACCTGGCATCCAACTGCCCGGT GGCAGCCCTGGAGATGAGGCGGGACCTGCAGCACTGGGACAGTGCTCTGCAGCTGGCGAAGCGCCTGGCC CCAGACCAGATTCCCTTCATCTCCAAAGAGTATGCCATCCAGCTGGAGTTCACAGGTGATTATGTAAATG CTTTGGCTCATTACGAGAAAGGCATCACCGGGGATAACAAGGAACATGATGAGGTGTGCCTGGCCGGAGT GGCTCAGATGTCTATAAGAATGGGAGACATCCGCAGAGGGGCAAACCAGGCCCTCAAACACCCCAGCAGG GTCCTGAAAAGAGATTGTGGTGCCATCCTAGAGAATATGAAGCAATTTTCAGAAGCTGCCCAGCTGTATG AAAAGGGCCAGTATTACGACAGAGCTGCCTCTGTCTACATCCGCTGCAAGAACTGGGCAAAAGTTGGCGA GCTTCTACCTCATGTCTCCTCTCCTAAGATCCACTTGCAGTATGCCAAAGCTAAGGAAGCAGACGGAAGG TACAAAGAAGCCGTGGTGGCCTATGAGAATGCAAAGCAATGGAACAGTGTCATCCGCATCTACCTGGACC ACCTCAACAACCCCGAAAAGGCCGTCAGCATCGTCAGAGAGACCCAGTCTCTGGACGGAGCCAAGATGGT AGCCAGGTTCTTTCTACAGCTTGGTGACTATGGATCCGCCATCCAGTTTCTGGTCCTGTCCAAATGTAAC AATGAAGCCTTCACCCTGGCTCAGCAGCACAACAAGATGGAAATCTACGCCGACATCATTGGTGCTGAAG ACACAACTAATGAGGACTATCAAAGCATCGCCTTATATTTTGAAGGAGAAAAGAGACATTTTCAGGCTGG AAAATTCTTCTTGCTGTGTGGCCAGTATTCACGGGCACTGAAGCACTTCCTGAAGTGCCCAAGCTCAGAA GATAATGTGGCCATAGAAATGGCGATCGAAACTGTGGGCCAGGCCAAAGACGAACTGCTCACCAATCAGC TGATAGACCACCTGATGGGGGAGAGTGATGGCATGCCAAAGGACGCCAAGTACCTGTTCCGCTTGTACAT GGCACTGAAACAGTACCGAGAAGCAGCCCGGACAGCCATCATCATCGCCAGAGAAGAGCAGTCTGCAGGC AACTATCGGAATGCACATGATGTTCTTTTCAGTATGTATGCAGAACTTAAATCCCAGAAGATCAAAATCC CCTCAGAAATGGCTACCAACCTCATGATCCTGCACAGTTACATACTCGTGAAGATTCACGTTAAGAGTGG AGACCATATGAAGGGGGCACGAATGCTCATTCGGGTGGCCAACAACATCAGCAAATTCCCATCGCACATC GTGCCTATCCTGACGTCTACGGTGATTGAGTGTCACAGGGCAGGCCTGAAGAACTCTGCATTCAGCTTTG CAGCCATGCTGATGAGGCCGGAATACCGCAACAAAATCGATGCCAAGTACAAAAAGAAAATTGAGGCGAT GGTCAGGAGGCCGGATACCTCAGAGACAGAAGAAGCCACGACCCCATGTCCCTTCTGCCAGTTTCTTCTC CCAGAATGTGAGCTGCTCTGCCCTGGCTGTAAAAACAACATTCCCTACTGCATCGCAACAGGCCGACACA TGCTGAAAGACGATTGGACAATGTGCCCACACTGTGGCTTCCCTGCGCTGTACTCAGAATTCAAGATATT GCTAAACAGTGAAAGCACGTGTCCCATGTGTTCAGAAAGATTAAACTCCAGTCAACTGAAAAAAATTACA GATTGCACCCAGTACCTACGAACAGAGACGGAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191679
Insert Size	4026 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191679.2, NP_001178608.2
RefSeq Size	4315 bp
RefSeq ORF	4026 bp
Locus ID	305349

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