Stk36 (NM_001173986) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217587 representing NM_001173986 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAAGTACCACGTTTTGGAGATGATTGGAGAAGGCTCTTTTGGGAGAGTGTATAAGGGCCGGAAAA AATACAGTGCTCAGGTGGTGGCCTTGAAGTTCATCCCCAAACTGGGGCGCTCAGAGAAAGAGCTGAGGAA TCTGCAACGAGAGATTGAAATCATGCGGGGTCTGTGGCATCCCAACATTGTGCATATGCTTGACAGCTTC GAGACTGACAAGGAGGTGGTGGTGGTGACAGACTATGCTGAGGGAGAGCTCTTTCAGATTCTGGAAGATG ATGGAAAACTTCCTGAAGAGCAGGTTCAGGCCATCGCCGCCCAGTTGGTGTCAGCCCTGTACTATCTGCA TTCCCACCGCATCCTACACCGAGACATGAAACCTCAGAACATTCTTCTCGCCAAGGGCGGTGGCATCAAA CTCTGTGACTTTGGATTTGCCCGAGCTATGAGCACCAACACCATGGTGCTGACCTCAATCAAAGGCACAC CGCTCTATATGTCTCCAGAACTGGTGGAGGAGCGACCATATGACCACACGGCAGACCTCTGGTCTGTAGG CTGCATCCTGTATGAGCTGGCTGTAGGCACACCTCCCTTCTACACCACCAGCATCTTTCAACTGGTTAGC CTCATTCTCAAGGACCCTGTGCGCTGGCCCTCCACCATTACTTCCTGCTTCAAGAACTTCTTGCAGGGGC TGCTCACTAAGGACCCCCGGCAGCGTCTGTCCTGGCCAGACCTCTTACATCACCCCTTTATTGCCGGCCG TGTCACCATAATAACCGAACCAGCAGGCTCCAACTTGGGCACCCCATTTACTAGTCGCCTACCCCCAGAA CTTCAGGTCCTAAAAGATGAACAGGCCCATCGACTGGCACCCAAGGGTAACCAGTCCCGCATCCTGCGCC AGGCCTGTAAACGCATGGCTGAAGAAGCCAAGCAGAAGGAAGACCAAAATACAGGATCCACCCTTGATCA AGAAGCTAGGCTCAGCAAGGTGACACCCAGCACAGCCCCTGTGCCTGGTCTAAAGGCCACTCCTCAGGAG TCAAGCCTCTTGGCTGGCTTACTGGCTTCAGAAATGAAGAACAACTGGGAAGACTGGGGGGCTGGAGAAG CACCCCCTACACCTCGGGAAAACCACATCACCTTGGAGTGTGAACAAGCCTTCCCTGAGCTGAGGCCAGA GACGATGGGTCGGCGGAGCACCGATGCAGTGGATCCTGAAAACGAGGAGCCAGACAGTGATGACGAGTGG CAACGCTTACTCGAGACTATGGAGCCAGTGCCTGTGCAGCTGAAGTCCCCCCTCACCCTGCTGTGTAATC CTGACTTCTGCCAGCATATCCAGAGTCAGCTGCGCGGGACTGGCGAGCAGATCCTGAAAGGCGTCCTGGA TGGTGCTTCCCACCTCCTTCCTGCACTCCGCATCCTGAGCAGTCTCCTGTCCAGCTGCAGTGACTCTGTG CTCTTGTATTCCTTCTGCCAGGAGGTGGGACTGCCCGAGCTGCCTCTCAGTCTTCTTAGGTACAGCCAGG AGAGCAGCAGTATCCAGCAGCAACCTTGGTACGGGGCCTTCTTACGGGAACTGGTGGCTGTGGTTCAGGC CTACTTTTCATGCACCTTCAATCTGGAGAGGAGCCAGACAGGTGACAGCCTACAGGTGTTTGAGGAGGCT GCCAGCCTCTTTTTGGACTTGTTGGGGAAGCTGTTGGCCCAACCAGATGATTCCGAGCCGACATTTCGAA GGGATAGCCTCATGTGCTTTGCTGTCTTGTGTGAGGCTGTGGATGGGAACAGCCGGGCCATCTCCAAAGC CTTTTATGCCAGCTTGTTGACCACACAGCGTGCTGTGTTGGACGGGCTCCTTCACGGCCTAACGGTTCCA CAACTCCCTTTCCACACACCACCAGGAGCCCCACAAGTGAGCCAGCCACTGCGGGAGCAGAGTGAAGATG TACCCGGAGCCATCTCTTCTGCCCTGGCAGCCATGTGCACTGCCCCTGTGGGGTTGCCCAGCTGTTGGGA CGCCAAGGAGCAGGTCTCTTGGCATTTGGCCAACCAGCTAACCGAGGACAGCAGCCAACTGAGGCCATCC CTCATCTCTGGTCTGCGGCACCATATCCTATGCCTCCACCTTCTCAAGGTTCTCTACTCCTGCTGCTATA TTAGTGAGCGCCTGTGCCATATTCTGGGACAAGAGCCTCTGGCCTTGGAGTCGCTGCTCATGTTAGTCCA GGGCAAGGTGAAAGTAGCAGACTGGGAAGAGTCCACTGAGGTGGCACTTTACCTCCTGTCCCTTCTTGTC TTTCGGCTCCAAGATCTACCTTCAGGGATGGAGAAGCTGGGCAGTGAGGTCGCTACTCTCTTCACCCACT CACACATCGTCTCTCTTGTGAATGCAGCAGCCTGTCTCTTAGGACAGCTTGGTCAGCAAGGGGTGACCTT TGACCTCCAGCCCCGGGAATGGATAGCTGCAGCTGCACATGCCTTGTCTGCCCCTGCAGAGGTCCGGCTG ACTCCACCGTGTAGTTGTGGCTTCTATGATGGCCTCCTCGTTCTTCTGCTTCAGCTCCTTACACAGGGGA AGCCTGGCCTGATCAGGGACGTGGCAGGTTCAGAGGTGTGGACCATTCTGTGGCACCGCTTTTCCATGGC TCTGAGACTATCTGAGGAGGTGTCTGCCCAGGAAGATGACCTGTCACTATCAAGTCCACCCAGCCTAGAG CCAGACTGGACACTGATTTCACCCCAAGGCATGGCAGCCTTGCTGAGCCTGGCAATGGCCATCTTCACCC AGGAGCCCCAGTTATGCCTGAGCCACCTGTCCCAGCATGGCAGTGTCCTCATGCTGACCCTGAAGCACCT GCTTTCACCCAGCTTCTTGCACCGCCTGAGCCAGGCGCCTCAGGGGTCTGAGTTTCTCCCCGTTGTGGTG CTCTCTGTGTGCAAGCTGCTCTGCTTCCCCTTCGCCCTGGATGTGGATGCTGACCTCCTCGGAGGCATCT TGGCTGACCTCAGGGCCTCAGAAGTTGCAGTCTACCTGCTGCAGGTCTGCTGCCACCACCTTCCATTGTT CCAAGCAGAGCTGCCCATTGGCCTCCTTACACGCCTGGCCCTCATGGATCCCACCTCTCTCAAGCAGTTT GTGAACACAGTAGCCACCTCTTCTAGAGCCATCATCTCATTCCTCTCTGTTGTTCTCCTGTGTGACCAAC CCCTCATGGTCTCTGACCTCCTGTCCCTGCTGACACACACAACCCGGATTCTATCCCCCAGCCACTTGTC CTTTATCCAGGAGCTCCTGTCTGGCTCTGATGAATCCTATCGGCCACTGCGCAGCCTCCTAGGCCACTCA GAGAATGCTGTGCGGGTCCGTACTTACAGGCTCCTGGGACATTTATTACAACACAGCATGGCCCTACGTG GGGCACTACAGAGCCAGTCAGGACTGCTCAACCTTCTGCTGCTAGGGCTTGGAGACAAGGACCCTGCCGT GCGACGCAGTGCCAGCTTTGCTGTGGGCAATGCAGCCTACCAGGCTGGTCCCTTGGGACCTGCCTTGGCA GCTGCAGTGCCCAGTATGACCCAGCTGCTGGGAGATGCTCAGGATGCTATCCGGCGCAATGCTGCATCAG CTCTAGGCAATCTGGGACCTGAAGGATTGGGCAAGGAGCTATTAAAGTGCCAAGTACCCCAGCGGCTCCT AGAAATGGCGTGTGGAGACCCTCAACCAACTGTCAAAGAGGCTGCCCTTATTGCCCTCCGGAGCCTCCGA CAGGAGCCCTGTATTCATCAGGTGCTGGTGTCGCTGGGTGCCAGTGAGAAATTAACCTTGCTGTCTTTGG GGCATCAGTTACTGCCAAGCAGCAATAGCAGGCCTACCTCTGCCAGACACTGCAGGAAGCTTATCCACCT CCTGAGGCCGACCCACAGCACATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001173986
Insert Size	3945 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001173986.1, NP_001167457.1
RefSeq Size	5031 bp
RefSeq ORF	3945 bp
Locus ID	301516

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