Npc1 (NM_153624) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Cdig2; SLC66A1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217572 representing NM_153624 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGCGCACCACCCGGCCCTCGGCCTCCTGCTGCTGCTGCTGCTGTGCCCTGCGCAGGTGTTCTCAC AGTCCTGTGTCTGGTACGGAGAGTGTGGAGTCGCCTTTGGAGATAAGAGGTACAACTGTGAATATTCGGG CCCACCGAAACCCCTCCCAAAGGACGGCTATGACTTAGTGCAGGAACTCTGTCCAGGACTCTTCTTTGGC AATGTCAGTCTTTGCTGTGACGTTCAGCAGCTTCAGACACTGAAGAGCAACCTGCAGCTGCCCCTGCAGT TTCTGTCCAGGTGTCCGTCATGTTTTTATAACCTGATGACCCTGTTTTGTGAGCTAACATGTAGCCCGCA CCAGAGTCAGTTTCTGAATGTGACAGCAACTGAAGATTATTTCGACCCCGAGACTCGGGAAAATAAAACA AATGTAAAGGAATTAGAGTACTACGTCGGACGGAGCTTTGCGAATGCAATGTACAATGCCTGCCGAGATG TGGAGGCGCCTTCCAGTAATGAGAAGGCCTTAGGACTCCTGTGCGGAAGGGATGCCCGTGCCTGCAATGC CACCAACTGGATTGAGTACATGTTCAACAAAGACAACGGGCAAGCACCGTTTACCATCATCCCCGTATTT TCAGATCTTTCGGTTCTTGGAATGGAACCCATGAGGAATGCCACCAAAGGCTGCAACGAGTCTGTAGATG AGGTCACAGGGCCGTGTAGCTGCCAGGACTGCTCTGCCGTCTGCGGCCCCAAACCCCAGCCCCCACCGCC CCCTGTGCCCTGGAGGATCTTAGGTTTGGACGCCATGTATGTCATCATGTGGGTCGCCTACATGGCGTTC CTGGTCCTGTTCTTTGGAGGACTGCTGGCAGTGTGGTGCCACAGAAGGCGGTACTTTGTGTCCGAGTACA CCCCCATTGACAGCAACATTGCCTTTTCCTCGAATAGCAGTGACAAAGGGGAAGCCTCGTGCTGCGACCC ACTTGGTGTAGCGTTTGATGACTGTCTGAGGCGCATGTTCACAAAGTGGGGGGCTTTCTGTGTCCGGAAT CCCACCTGCATCATTTTCTTCTCGTTGGTCTTCATTGCTGCGTGCTCTTCTGGCCTGGTGTTTGTCCAGG TCACCACCAATCCCGTGGAGCTCTGGTCAGCCCCTCACAGTCAGGCCCGCTTGGAAAAGGAGTACTTTGA CAAGCACTTTGGGCCTTTCTTTCGTACGGAGCAGCTTATTATCCAGGCCCCCAACTCCAGCGTCCATATC TACGAGCCGTACCCTTCCGGAGCCGATGTGCCCTTCGGGCCTCCATTGAACAAAGAGATTCTGCACCAGG TTCTGGACTTACAGATCGCCATTGAAAGTATCACTGCGTCCTATAACAATGAAACCGTGACACTTCAGGA CATCTGTGTGGCCCCCCTCTCTCCATACAACAAGAACTGCACCATTATGAGTGTGTTAAATTACTTCCAG AACAGCCATGCGGTGCTGGACAACCAAGTAGGCGATGACTTCTTTGTCTATGCCGATTACCACACGCACT TTCTGTACTGTGTGAGGGCTCCCGCCTCCTTGAATGATACGAGTTTGCTCCACGGCCCTTGCCTGGGCAC ATTTGGAGGACCTGTGTTCCCGTGGCTTGTGTTGGGCGGCTATGATGATCAGAACTACAATAATGCCACC GCGCTTGTGATTACCTTCCCTGTCAATAATTACTATAATGACACAGAGAAGCTCCAGAGGGCCTGGGCCT GGGAGAAAGAATTTATTAACTTTGTGAAGAACTACAAGAATCCAAATCTGACCATTTCTTTCACTGCTGA GCGAAGCATTGAAGATGAACTCAATCGGGAAAGCAACAGTGACGTATTCACCGTCCTCATCAGCTATGCC GTGATGTTTCTGTACATTTCCCTCGCCCTGGGGCACATCCAGAGCTGGAACAGGGTCCTGGTGGATTCTA AAATCTCGCTGGGCATTGCGGGGATCCTGATAGTGCTGAGCTCGGTGACCTGCTCCCTGGGCATCTTCAG CTACATCGGAATGCCACTGACCCTCATCGTCATTGAGGTCATCCCGTTCCTGGTGCTGGCTGTTGGGGTG GACAACATCTTCATTCTAGTGCAGACCTACCAGAGAGATGAGCGTCTTCAGGAGGAGACGTTGGATCAGC AGCTGGGCAGGATCCTGGGGGAAGTGGCCCCAACCATGTTCCTTTCGTCCTTCTCTGAGACCTCGGCATT TTTCTTTGGGGCGCTGTCCTCGATGCCAGCTGTCCACACCTTCTCTCTGTTTGCGGGGATGGCCGTCCTC ATTGACTTCCTCCTTCAGATTACCTGCTTTGTGAGCTTCTTGGGGTTAGATATTAAGAGACAAGAGAAAA ACCGTCTGGACATCCTGTGCTGTGTCAGAGGCCCTGACGACGGACAAGAATCCCAAGCCTCCGAAAGCTA CCTGTTCCGCTTCTTCAAAAACGCCTTTGCCCCATTTCTGCTGACGGACTGGCTGCGGCCAGTTGTGATG GCCGTGTTTGTGGGCATTCTGTCATTCAGTATTGCAGTAGTGAACAAAGTAGAAATCGGATTGGATCAGT CTCTCTCGATGCCAAATGATTCGTATGTGATTGACTATTTCAAGTCACTCGGTCAGTACCTGCACTCGGG CCCACCCGTGTACTTCGTCCTGGAAGAGGGCTACAACTACAGTTCCCGCAGAGGGCAGAACATGGTGTGC GGTGGCATGGGCTGTGACAATGACTCCCTGGTGCAGCAGATATTTAACGCAGCAGAGCTGGACACCTACA CCCGCGTAGGCTTCGCTCCCTCATCCTGGATTGATGATTACTTTGACTGGGTCTCACCGCAATCCTCCTG CTGTAGACTCTACAATGTCACTCACCAGTTCTGCAATGCTTCTGTGATTGACCCAACCTGTGTCCGCTGC AGACCTCTGACTCCAGAGGGCAAACAGAGGCCTCAGGGGAAAGAATTCATGAAATTCCTGCCCATGTTCC TGTCTGATAACCCCAACCCCAAGTGTGGCAAAGGGGGACATGCTGCTTACAGTTCTGCTGTTAACATCAT GGGAGATGACACTTACATTGGGGCCACTTACTTCATGACCTACCACACCATTCTTAAGACCTCTGCTGAT TATATCGATGCCCTGAAGAAAGCTCGGCTTATAGCCAGTAACATCACGGAAACCATGCGTTCTAAGGGGA GTGATTATCGTGTGTTTCCTTACAGTGTGTTCTATGTCTTCTATGAACAATACCTGACCATTATTGATGA CGCCATCTTTAACCTAAGTGTGTCTCTGGGCTCCATATTTCTCGTGACCTTGGTGGCTCTGGGCTGTGAA CTGTGGTCTGCGGTCATCATGTGTCTCACCATAGCTATGATCCTGGTCAACATGTTTGGTGTTATGTGGC TGTGGGGCATCAGTCTGAACGCTGTTTCCTTGGTCAACTTGGTGATGACCTGTGGCATTTCTGTGGAGTT CTGCAGCCATATAACAAGAGCTTTCACCATGAGCACCAAAGGAAGCAGAGTGAGCAGGGCGGAAGAGGCA CTGGCCCACATGGGTAGTTCTGTATTCAGTGGAATCACACTTACAAAATTTGGAGGGATCGTGGTGTTGG CCTTTGCCAAGTCTCAAATTTTTGAGATCTTTTACTTCAGGATGTACTTAGCCATGGTCTTACTTGGAGC TACTCACGGACTAATATTTCTTCCCGTCTTACTCAGTTACATAGGGCCATCAGTGAATAAAGCTAAAAGA CACACCACTCAAGAGCGGTACAAAGGGACAGAGAGAGAACGGCTCCTCAATTTTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_153624
Insert Size	3837 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_153624.2, NP_705888.2
RefSeq Size	5161 bp
RefSeq ORF	3837 bp
Locus ID	266732
Gene Summary	cadmium-inducible gene; transcription upregulated by cadmium [RGD, Feb 2006]

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