Anks1b (NM_001271371) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	AIDA-1; EB-1; RGD1565556
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217566 representing NM_001271371 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAAGGACCAGGAGCTGCTAGAGGCTGCTCGTACTGGCAATGTGGCTCTGGTCGAGAAACTCCTAT CTGGCAGGAAAGGAGGGATCCTGGGTGGTGGATCCGGACCTCTGCCCCTGTCTAATCTGCTAAGCATCTG GCGAGGTCCCAACGTGAACTGCACAGACAGTTCGGGTTACACCGCTTTGCACCACGCAGCCTTAAATGGA CATAAGGACATAGTTCTCAAACTACTTCAATTCGAGGCGTCCACCAATGTCGCAGACAACAAAGGTTACT TCCCCATTCACCTGGCTGCCTGGAAAGGAGACGTGGAGATTGTGAAGATTCTTATCCATCACGGGCCTTC ACATTCCAGAGTCAATGAACAGAACAACGAGAACGAAACTGCCCTCCACTGTGCAGCGCAATACGGACAC TCAGAAGTAGTGGCTGTTCTCCTAGAGGAGCTCACCGATCCCACCATTAGGAACAGCAAGCTCGAAACAC CGCTGGACTTGGCAGCGCTCTACGGAAGGCTGAGGGTGGTGAAGATGATCATCAGCGCGCACCCAAACTT AATGAGCTGTAACACGCGGAAGCACACGCCGCTCCACCTCGCGGCCCGCAATGGTCACAAGGCCGTGGTG CAGGTGCTGCTGGAGGCGGGGATGGACGTGAGCTGTCAAACAGAGAAGGGGAGTGCTCTTCACGAAGCGG CGTTGTTTGGGAAGGTGGATGTGGTGCGAGTTCTGTTAGAAACAGGAATTGATGCCAACATAAAGGACAG CCTAGGTAGAACCGTCTTAGACATTCTGAAAGAGCACCCGTCTCAGAAGTCTCTCCAGATTGCAACGCTC CTGCAAGACTATTTGGAAGGCGTGGGAAGGTCGGTGGTCCTCGAGGAAGAGCATGCTCAGGAAGACACAG CACAAGAGACACGCCTTTCGTCTCCTGCCCAGTCTCCTTCTCAAAAGACTAAAAGTGAAACTGTCACTGG AGAACTATCGAAACTCCTGGATGAAATAAAACTCTGTCAAGAAAAGGATTATTCCTTTGAAGATCTGTGC CATACAATCTCAGACCACTACTTAGATAATTTGAGCAAGATCTCAGAGGAAGAACTTGGGAAAAATGGAA GCCAGAGTGTAAGAACTTCATCTACAATAAATTTGTCACCAGGAGAAGTGGAAGACGAAGAGGAGGATCC AAACTCTTGTGGACCCACGGGACTTTGGGAAGCGCTAACTCCTTGTAACGGATGTAGGAACCTTGGCTTT CCCATGCTCGCACAGGAGTCTTATCCAAAGAAGAGGAATTACCCCATGGAAATTGTACCATCTGCTTCTC TGGACACATTCCCTTCAGAAAATGAGAACTTTCTTTGTGAACTCGTGGACACAGCTGTTACAAAGAAGCC CTGTTCCTTAGAAATCGCAAGGGCACCTTCTCCGAGAACTGATAATGCCTCTGAGGTAGCAATTACTGCT CCAGGAACTGGTCACCATAGAAACAGCTCTACAGGCCCAACACCTGACTGCTCACCTCCATCCCCCGACA CTGCCCTAAAAAATATCGTAAAAGTTATCCGACCTCAGCCAAAACAACGAACATCTATTGTGTCGTCTCT GGATTTCCAGCGAATGAATCATAACCAAGAATACTTTGAAATCAGCACATCTACAGGCTGTACCAGCTTT ACTTCGAGCCCTCCAGTGAGCCCACCCACCTCCTCTGTGGAAACCACAGAAATCAAGAACGAGGGAGCTG AACACACAGATGACCTCTCTCAGCAGGAAGATGACGAACCCCCAAAAGAGTATGATGCTGGGCAATTCGC AGGCCTTCTCCACGGGTCCTCTCCAGCCTGTGAGGCTCCAGAAAATCCGTTTCACCTGTATGGGAAAAGA AATCAAGGTGAAGACGGACAAGAGGAAGCCAGCTTGGCCAACAGTCCTTTGCCTTTCAAACAGACTCCAA TAGAAAATAACCCAGAGCCTTCAGTGAAGAAAATTAAACCCAAAGTGGTCAGTAGAACGATTTTCCACAA AAGAAGCCACCAACTTGAGAACCATACCATCGTTGGCACAAGAATGTCCAGGGGTGGATCCCGCAACGGC GACCAATGGGGTGTGAATCCAGGGGGCTTCGTGGAGAGAGCGTGCACTCTAGGGAGAATAAGGTCATTGC CTAAAGCCCTGATCGACATGCATTTATCCAAAAATGTCTCCAAGTCCGATTCCGATCTTATTGCGTACCC TTCCAAGGACAAAGCGAGAGTTAACTGGAGCAAATCGTCAACTGCAGAGCGCAGTTCCAAAGATAATTCT GAAAGAACGCCTTCCTTCACATCTGAATGGGAAGAAATCGACAAAATAATGAACTCGATTGACGTTGGAA TCAACAGCGAACTTGAAGGGATGAACGGACCCAGATGCCCTGTCCAAACAGTAGGACAATGGTTGGAAAG CATTGGGCTACCTCAGTACGAGAACCACCTGACGGCTAATGGATTTGACAATGTACAGTTTATGGGAAGC AATGTTATGGAAGATCAGGATTTGTTGGAAATTGGAATCCTTAACTCTGGTCACAGACAAAGAATTCTAC AGGCGATCCAGCTGCTTCCAAAGATGAGGCCCATCGGCCACGATGGCTACCATCCCACCTCTGTAGCTGA GTGGCTGGATTCCATCGAGCTGGGCGACTACACGAAAGCCTTTCTCATCAATGGCTACACGTCGATGGAC CTGTTGAAAAAGATCTGGGAGCTTGAACTTATTAACGTTTTAAAAATCAGTTTGATTGGCCACAGGAAGC GCATTTTGGCATCTCTGGGAGACAGACTGCACGAGGACCCTCCACAGAAGCCCCCTCGGTCCATCACCCT CAGGGAACCCAGTGGTAATCACACTCCTCCTCAGTTGTCTCCATCACTTAGCCAAAGCACTTACACCACT GGTGGCTCCCTAGACGTCCCTCACATTATCATGCAGGGCGATGCAAGGAGGAGAAGAAATGAAAACTACT TTGATGATATTCCCCGATCAAAGCTGGAGAGACAGATGGCTCAGACAGGGGACTGGGGAGAACCTTCCAT CACCTTGCGACCTCCTAATGAAGCCACAGCCTCAACTCCAGTCCAGTACTGGCAGCATCACCCAGAAAAG CTCATCTTCCAGTCGTGTGACTACAAAGCCTTTTATTTAGGTTCTATGCTGATAAAAGAGCTGAGGGGAA CGGAATCAACACAGGACGCTTGCGCAAAGATGCGGGCTAACTGTCAGAAGTCTACGGAGCAAATGAAGAA AGTCCCCACTATCATTCTTTCTGTCTCATATAAAGGAGTCAAATTTATTGATGCGGCAAATAAGAACATA ATTGCCGAGCATGAAATTCGTAATATCTCTTGTGCTGCCCAGGACCCAGAAGACCTCTCAACCTTTGCTT ATATCACAAAAGATCTGAAGTCCAACCACCACTACTGTCACGTGTTTACTGCCTTTGATGTGAATTTAGC CTATGAAATCATCCTAACGCTGGGGCAGGCATTTGAGGTCGCTTACCAGCTAGCTCTACAAGCAAGAAAA GGGGGCCATTCCTCCACGCTTCCGGAGAGCTTTGAGAACAAGCCCTCGAAACCCATCCCAAAGCCCCGAG TTAGCATTCGCAAGTCAGTGCAGATCGACCCTTCTGAGCAAAAGACTCTGGCCAATCTGCCGTGGATTGT GGAGCCGGGGCAAGAAGCCAAGAGGGGCATTAATACCAAGTATGAAACCACGATTTTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271371
Insert Size	3771 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271371.1, NP_001258300.1
RefSeq Size	5579 bp
RefSeq ORF	3771 bp
Locus ID	314721
Gene Summary	Isoform 2 may participate in the regulation of nucleoplasmic coilin protein interactions in neuronal and transformed cells.[UniProtKB/Swiss-Prot Function]

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