Rasgrf1 (NM_001170531) Rat Untagged Clone

CAT#: RN217561

Rasgrf1 (untagged) - Rat RAS protein-specific guanine nucleotide-releasing factor 1 (Rasgrf1), transcript variant 1



  "NM_001170531" in other vectors (1)

CNY 11,970.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms GNRP; P140 RAS-GRF
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN217561 representing NM_001170531
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGAAAGCCATCCGACTGAACGATGGCCACGTCGTGTCCCTGGGACTGCTGGCCCAGAGAGACGGCA
CGCGCAAAGGCTACCTGAGCAAGAGGAGTTCGGACAACCCAAAATGGCAAACCAAGTGGTTTGCGCTGCT
GCAGAACCTGCTCTTCTACTTCGAAAGTGACTCGAGCTCTCGGCCCTCGGGGCTCTACCTGCTGGAGGGC
AGTATCTGCAAACGCATGCCCTCCCCCAAGCGAGGGACCTCCTCCAAGGAGTCCGACAAACAGCATCATT
ACTTCACAGTGAACTTCTCCAATGACAGCCAGAAGTCCCTAGAGCTGAGGACCGATGACTCCAAGGACTG
TGACGAGTGGGTGGCAGCGATTGCTCGCGCCAGCTACAAGATACTGGCCACAGAGCATGAGGCGCTCATG
CAGAAGTACCTGCACCTGCTGCAGGTGGTGGAGACAGAGAAGACCGTGGCTAAGCAGCTGCGACAGCAGC
TCGAGGATGGCGAGGTCGAGATCGAGCGCCTGAAGGCAGAGATTGCAAACCTGATCAAGGACAATGAACG
TATCCAGTCCAACCAGCTGGTTGCCCCTGAGGATGAGGACAGTGACATCAAGAAAATTAAGAAGGTACAG
AGTTTCCTTCGCGGATGGCTGTGCCGGCGAAAGTGGAAGAACATCATCCAGGACTACATCCGGTCTCCTC
ATGCCGACAGCATGCGCAAGAGGAACCAGGTGGTGTTCAGCATGCTGGAAGCTGAGGCCGAGTACGTGCA
GCAACTACACATCCTTGTCAACAATTTTCTGCGCCCACTGCGCATGGCCGCCAGCTCTAAGAAACCCCCT
ATAACACATGACGACGTCAGCAGTATCTTTCTGAACAGTGAGACCATCATGTTCCTGCACCAGATCTTCT
ACCAAGGCCTGAAGGCCCGTATCGCCAGCTGGCCCACCCTGGTTCTGGCGGACCTGTTCGACATCCTGCT
GCCAATGCTTAACATCTACCAGGAGTTCGTCCGCAACCACCAGTACAGTCTCCAGATCCTAGCACACTGC
AAGCAAAACCGGGACTTTGACAAGCTCCTCAAGCAGTATGAGGCCAAGCCAGACTGCGAGGAGCGCACAC
TGGAGACCTTCCTCACCTATCCAATGTTCCAGATCCCCAGGTACATCCTGACACTCCATGAGCTGCTGGC
CCACACACCTCATGAGCATGTGGAGCGCAACAGCCTGGACTATGCCAAATCCAAACTAGAGGAGCTGTCC
AGGGTCATGCACGACGAAGTCAGTGAGACCGAGAACATCCGCAAAAACCTGGCCATTGAGCGTATGATCA
CCGAGGGCTGTGAGATCCTCCTTGACACCAGCCAGACCTTTGTGCGCCAAGGTTCCCTCATCCAGGTGCC
CATGTCAGAAAAGGGCAAGATCAACAAGGGCCGCCTGGGGTCTCTGTCCCTTAAGAAAGAAGGTGAGCGC
CAGTGTTTCCTGTTCTCCAAGCATCTCATCATCTGCACCAGAGGCTCTGGTAGCAAACTGCACCTAACCA
AGAATGGCGTGATTTCCCTCATTGACTGCACTCTACTGGATGATCCAGAAAACATGGATGATGACGGCAA
AGGACAAGAGGTAGATCACCTGGACTTTAAGATTTGGGTGGAGCCAAAGGATTCCCCACCCTTCACAGTC
ATCCTGGTGGCCTCATCCAGGCAGGAGAAGGCGGCATGGACCAGTGACATCATCCAGTGCGTGGATAATA
TCCGCTGCAACGGGCTCATGATGAATGCCTTTGAAGAAAATTCCAAGGTCACCGTGCCGCAGATGATCAA
GTCTGATGCTTCCTTATACTGTGATGATGTTGACATTCGCTTCAGCAAAACCATGAATTCTTGCAAAGTG
CTGCAGATCCGCTATGCCAGCGTGGAGCGCCTGCTGGAGCGCCTGACTGATCTTCGCTTCCTGAGTATTG
ACTTTCTCAACACCTTCCTGCACTCCTATCGAGTCTTCACCGATGCTGTGGTGGTCCTAGACAAGCTGAT
CAGCATCTACAAAAAGCCCATCACTGCGATTCCTGCCAGGTCACTGGAACTCCTGTTCTCCAGTAGCCAC
AACACCAAACTTCTGTACGGAGATGCCCCCAAGTCGCCTCGTGCCAGCCGCAAGTTCTCCTCGCCGCCGC
CCTTGGCCATCGGCACTTCGTCCCCAGTCCGCCGCCGGAAGTTGTCTCTCAACATTCCCATCATCACAGG
CGGCAAGGCGCTGGAACTGGCTTCGCTCGGGTGCCCCTCCGACGGCTACACCAACATACACTCGCCCATA
TCTCCCTTCGGCAAAACCACGCTGGACACCAGCAAGCTCTGTGTGGCCAGCAGCTTGACCAGAACGCCGG
AGGAGATTGATATGACCACTCTAGAGGAGTCATCAGGCTTCAGGAAGCCGACCTCAGACATCTTGAAAGA
AGAGTCTGATGATGACCAGAGTGATGTAGACGACACAGAAGTGTCTCCACCAACACCGAAATCATTCAGA
AACAGAATCACTCAAGAGTTCCCACTCTTTAACTACAACAGTGGAATCATGATGACATGTCGCGATCTGA
TGGACAGTAACCGCAGCCCTCTGTCAGCTACCTCTGCCTTTGCCATAGCGACTGCAGGAGCCAATGAAAG
CCCCGCAAACAAGGAGATATATCGAAGGATGTCTTTGGCCAACACAGGGTATTCCTCTGACCAGAGAAAT
ATCGACAAAGAGTTCGTGATCCGCAGAGCGGCCACCAACCGTGTACTGAATGTGTTGCGCCACTGGGTCA
CCAAGCACTCCCAGGACTTTGAAACTGACGACCTCCTCAAATACAAGGTGATCTGCTTTCTGGAAGAGGT
CATGCATGACCCAGACCTTCTACCACAAGAGCGAAAGGCAGCAGCCAACATCATGAGGACTCTGACCCAG
GAAGAAATAACTGAAAACCATAGCATGCTGGATGAGCTCTTACTAATGACGGAGGGTGTGAAGACTGAGC
CCTTCGAAAACCACTCAGCCATGGAGATAGCAGAGCAGCTGACCCTGCTGGATCACCTTGTCTTCAAGAG
TATTCCTTATGAGGAATTCTTTGGCCAGGGCTGGATGAAGGCAGATAAGAATGAAAGGACACCTTACATT
ATGAAAACCACCAGACATTTCAACCATATCAGTAACTTGATCGCTTCAGAAATTCTCCGAAACGAGGAGG
TCAGTGCAAGGGCAAGCACCATCGAGAAGTGGGTGGCTGTTGCCGACATTTGCCGCTGCCTGCACAACTA
CAATGCTGTGCTGGAGATCACTTCCTCCATCAACCGCAGCGCAATCTTCCGACTCAAGAAGACATGGCTC
AAAGTTTCTAAGCAGACGAAATCTCTGTTTGACAAGCTCCAAAAGCTTGTGTCATCAGATGGCCGATTTA
AGAACCTCAGAGAAACTTTGCGAAATTGTGATCCACCCTGTGTCCCTTACCTGGGGATGTACCTGACCGA
CTTGGCATTCCTCGAGGAAGGAACACCCAATTACACAGAGGACGGCCTGGTCAACTTCTCCAAGATGAGG
ATGATCTCCCATATTATCCGCGAGATTCGCCAGTTTCAGCAGACTACTTACAAAATCGAGCCCCAGCCAA
AGGTAACTCAGTACTTAGTGGATGAAACCTTTGTGTTGGACGACGAAAGTCTGTATGAGGCCTCCCTCCG
AATTGAACCAAAACTCCCCACATGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001170531
Insert Size 3735 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001170531.1, NP_001164002.1
RefSeq Size 4226 bp
RefSeq ORF 3735 bp
Locus ID 192213
UniProt ID P28818
Gene Summary a paternally expressed imprinted gene [RGD, Feb 2006]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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