Pcdh12 (NM_053944) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217527 representing NM_053944 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTACTTCTGCCTTTCCTGCTAGGGCTCTTAGGACCAGGAAGCTACTTGTTCATTTCAGGGGCTTGTC AGGAGGTGGCCACTGTCACGGTGAAATTCCAAGTGGCAGAGGAAGTGCCATCTGGCACCGTGATAGGGAA ACTGTCCCAAGAACCAGGAGTGGAGGAGAGGCGTGGGAAGGCAGGAGATGCCTTTCAGATTCTGCAGCTG CCTCAGGCACTGCCGATTCAGATGAACTCTGAGGATGGCCTGCTCAACACTTCCAGCCGGCTGGATCGGG AGAAGCTCTGTCGGCAGCAAGATCCCTGCCTGGTGTCCTTTGATGTGCTTGCCACAGGGGCTTCTGCTCT AATTCATGTGGAGATTCAGGTGCTGGACATCAATGACCACCAGCCTCAGTTTCCCAAAGATGAGCAGGAG CTGGAAATCTCTGAGAGTGCTTCTCTGCACACACGAATTCCCTTGGACAGAGCTCTTGACCAAGACACTG GCCCTAACAGCTTATATTCCTATTCCCTGTCTCCCAGTGAACACTTTGCCCTGGATGTTATTGTGGGCCC TGATGAGACCAAACACGCAGAACTTGTGGTGGTGAAGGAGTTGGACAGGGAAGTCCACTCATATTTTGAT CTGGTGCTGACTGCCTATGACAATGGGAATCCCCCCAAGTCAGGTACCAGCGTGGTCAAGGTCAATGTCC TAGATTCCAATGACAATAGCCCAGTGTTTGCTGAGAGTTCACTAGCACTAGAAATCCCAGAAGACACTGC CCCTGGTACTCTTCTCATAAACCTGACTGCTACGGATCCAGACCAAGGCCCCAATGGGGAGGTAGAATTC TTCTTTGGCAAGCATGTGTCCCCAGAGGTGATGAATACCTTTGGTATAGATGCTAAGACAGGCCAGATCG TTCTGCTCCAACCCCTAGACTACGAGAAAAACCCTGCCTATGAGGTGGATGTCCAGGCAAGGGACTTAGG TCCCAATTCCATCCCAGGCCATTGCAAAGTTCTCATCAAAGTTCTGGATGTCAATGACAACGCCCCAAGC ATCCACATCACGTGGGCCTCCCAGATGTCGCTGGTGTCAGAAGATCTTCCCAGGGACAGCTTCATTGCTC TTGTCAGTGCGAATGACTTGGACTCAGGAAACAATGGTCTAGTCAACTGTTGGCTGAATCAAGAGCTAGG TCACTTCAGACTGAAAAGGACTAATGGCAACACGTACATGCTGCTCACCAGTGCCATGCTGGACAGAGAG CAATGGCCCACATACACTCTCACTGTGTTTGCCCAAGACCAAGGACCCCAGGCCTTATCAGCTGAGAAGG AGCTCCAGATTCAGGTCAGTGATGTCAATGACAATGCCCCTGTATTTGAGAAGAGCCGGTATGAGGTCTC CACTTGGGAAAACAACCCCCCCTCTCTTCACCTTATCACGCTCAAAGCTCATGATGCTGACTTGGGCAGT AATGGAAAAGTGTCATACCGTATCAAGGACTCCCCTGTTTCTCACTTAGTCATTATTGACTCTGAAACAG GAGAAGTCACTGCTCAGAGGTCGCTGGACTATGAAGAGATGGCAGGCTTTGAGTTCCAGGTGATAGCAGA GGACAGAGGGCAACCCCAGCTCTCATCCAGCATCTCGGTGTGGGTTAGCCTCTTGGATGCCAACGACAAT ACCCCAGAAGTGATTCAGCCTGCGCTCAGTGAAGGCAAAGCCACCCTTTCAGTGCTTGTAAATGCCTCCA CGGGCCACCTTCTGTTGCCCATTGAGAGCCCCAGTGGCATGGATCCAGCAGGTACTGGTCCAACACCACC AAAGGCTACCCACAGCCCCTGGTCCTTCCTTTTGGTGACAATCGTGGCAAGGGATGCAGACTCGGGGGCC AATGGGGAACTCCTTTACAGCATTCAAAGTGGGAATGATGCTCATCTCTTTGTCCTCAGCCCTTCCCTGG GGCAGCTATTCATTAATGTCACCAATGCCAGCAGCCTCATCGGGAGTCAATGGGACCTAGGCATAGTGGT AGAGGACCAGGGGAGCCCCTCCTTGCAGACCCAAGTTTCACTGAAGGTCGTGTTTGTCACCAGCATGGAC CACCTAAGGGACTCTGCTCACGAGCCCGGAATTCTGAGCACACCAGCGCTGGCTATGATCTGCCTGGCTG TACTGCTGGCCGTCTTTGGATTGATCTTGGCTTTGCTTGTATCCATCTGCCGGATAGAGAAAAGGGATAA TAGGGCCTACAACTGTCGGGAAGCTGAGTCTATGTACCGCCACCAGCCCAAGAGGCCCCAGAAACACATT CAGAAGGCAGATATCCACCTGGTGCCTATGCTTAGGGCCCAGGAGGATGAGGCTGAGGAAGTTAGGCCAC CTCACAAGGATACCAGTAAAGAGACATTGATGGAGGCAGGTTGGGACTCCTGCCTACAGGCCCCCTTCCA CCTCACACCAACCCTATACCGGACCCTGCGTAACCAAGGCAACCAGGGAGCACTGGCAGAGAGCCAGGAG GTACTGCAGGACACCTTCAACTTTCTCTTCAACCACCCCAGGCAGAGGAATGCCTCCCGAGAGAACCTCA ACCTTCCTGAGTCCCCACCCACCCTACGCCAGCCACTCTTAAAGCCTCTGAAGGTGCCTGGCAGCCCCAT AGCGAGGGTGACTGGAGACCAAGGCAGCAAGGAGGCACCACAGAGCCCACCAGCGTCCTCTGCAACCCTA AGACGACAGAGGAATCTCAATGGCAAAGTGTCTCCTAAAGGCGAGTCCAGTCCTCATCAGATTCTCAGGA GCCTGGTTCGACTCTCTGTAGCTGCTTTTGCAGAACGGAACCCTGTGGAGGAGCTTGCTGGGGACTCTCC TCCTGTTCAGCAAATCTCCCAGCTGCTGTCCTTGCTGCATCAGGGCCAATTCCAGCCCAAACCAAACCAC CGAGGAAATAAATACTCGGCCAAGCCCGGCAGCAGCAGAGCTACCATCCCAGACACAGATGGCTTAGGCA TCAGGCCTGGTGGCCTATCAGAACCTGACTTGGAAGAAGGGCCCCCAAGCCCAGAGGAGGATCTTTCTGT AAAGCGACTTCTAGAAGAAGAGTTGTCCAGCCTGTTGGATCCTAATACAGGTCTAGCCCTGGACAAGCTG AGTCCCCCTGACCCAGCCTGGATGGCGAGACTGTCATTGCCCCTCACTACCAATTACCGAGACAACTTGT CTTCCCCTGATGCTGCAGCATCAGAGGAACCGAGAACCTTCCAGACATTCGGCAAGACAGTCGGACCCGG TCCCGAGCTAAGCGCAACAGGCACGCGCCTGGCCAGCACTTTCGTCTCGGAGATGAGCTCACTGCTGGAG ATGTTGTTGGAGCAGAACACGGTGCCTGTGGAAGCTGCGTCTGCGGCTTTGCGGAGGCTCTCAGTGTGCG GGAGGACCCTGAGTCTAGACCTAGCCACCAGTGGGGCCTCGGGCTCAGAAGCACAGCTGGGTAGAAAAAA GGCAGCTGAGAGCAGTCTTGGCTGTGGCAGGAATCTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053944
Insert Size	3540 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053944.1, NP_446396.1
RefSeq Size	4183 bp
RefSeq ORF	3540 bp
Locus ID	116808
Gene Summary	may play a role in cell-cell adhesion during spermatogenesis [RGD, Feb 2006]

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