Thbs2 (NM_001169138) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	TSP-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217522 representing NM_001169138 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCTGGGCACTGGCCCTGCTGGCTCTGGGCATAGGGCCAAGAGCTTACGCTGGTGACCACGGCGAGG ACACAGCATTTGACCTTTTCAGCATCAGCAACATTAACCGGAAGACCATCGGTGCCAAGCAGTTCCGAGG GCCCGACCCCGGTGTGCCCGCCTACCGTTTTGTCCGGTTTGACTACGTCCCCCCAGTGAATACAGACGAC CTCAACAGGATCGTTAAGCTTGCAAGGAGAAAGGAGGGCTTCTTCCTCACAGCCCAGCTGAAGCAGGACC GCAAGTCTCGGGGAACACTCCTGGTGTTGGAAGGTCCCGGCACCTCCCAGAGGCAGTTTGAGATTGTGTC CAATGGCCCAGGGGATACCTTGGACCTCAACTACTGGGTAGAAGGCCACCAGCATACCAACTTCCTGGAG GATGTGGGTCTGGCTGACTCCCAGTGGAAGAATGTGACTGTGCAGGTGGCCAGTGACACCTATAGCCTGT ATGTGGGCTGTGATCTTATTGACAGTGTCACCCTGGAAGAACCCTTCTATGAGCAGCTGGAAGCAGACAA GAGCAGGATGTACGTGGCCAAGGGTGCATCTCGAGAGAGTCACTTCAGGGGCTTGCTGCAGAATGTCCAT CTCGTGTTTGCAGATTCTGTGGAAGATATTCTAAGCAAGAAAGGCTGTCAACACAGCCAGGGAGCTGAAG TCAACACCATCAGTGAACATACAGAGACACTCCACCTGAGCCCTCACATCACCACAGATCTGGTGGTCCA GGGTGTGGAGAAGGCACAGGAGGTGTGTACGCACTCCTGTGAAGAGCTGAGCAACATGATGAACGAGCTG TCCGGATTGCACGTCATGGTGAACCAGCTGAGCAAGAACCTGGAGAGAGTGTCTAGCGATAACCAGTTCC TTTTGGAGCTCATTGGGGGCCCTCTGAAGACAAGAAACATGTCGGCCTGTGTGCAGGAGGGACGGATCTT TGCAGAAAACGAAACCTGGGTTGTGGATAGTTGTACCACCTGCACCTGCAAGAAATTTAAAACAGTCTGC AATCAAATCACCTGCTCACCTGCAACTTGTGCCAACCCATCTTTGGTGGAAGGCGAGTGCTGTCCATCCT GTTCCCACTCTGCAGACAACGATGAGGGCTGGTCTCCGTGGGCAGAGTGGACCGAGTGTTCTGTCACCTG TGGCTCTGGGACCCAGCAGAGAGGCCGGTCTTGTGATGTCACCAGCAACACCTGCCTGGGCCCCTCCATT CAGACAAGGACATGCAGTTTGGGCAAATGTGATACGAGAATCCGTCAGAATGGAGGCTGGAGTCACTGGT CACCCTGGTCTTCGTGCTCCGTGACTTGTGGAGTTGGCAATGTCACCCGCATACGTCTCTGCAACTCACC AGTGCCCCAAATGGGTGGCAAGAACTGCAAGGGCAGTGGCCGGGAAACCAAGGCCTGCCAGCGTGCTCCA TGCCCAATTGATGGCCGCTGGAGCCCCTGGTCCCCTTGGTCAGCCTGCACAGTTACCTGTGCTGGAGGGA TCCGTGAGCGCACACGTGTTTGCAACAGCCCTGAGCCCCAGTATGGAGGGAAGGATTGTGTCGGGGATGT GACAGAACACCAAATGTGCAACAAGAGGAGCTGCCCTATTGATGGATGCCTATCCAACCCGTGTTTCCCT GGAGCCAAGTGCAACAGCTTCCCCGATGGCTCCTGGTCCTGTGGTTCCTGCCCAGTGGGCTTTCTGGGCA ATGGCACCCACTGTGAGGACCTGGATGAGTGTGCTGTGGTCGCAGATATCTGCTTCTCAATTAACAAAGC TTCCCGCTGTGTCAACACCAACCCTGGCTTCCACTGCCTGCCTTGTCCGCCACGCTACAAGGGGACCCAA CCCTTCGGGATTGGCCTGGAGGATGCTAAAACAGAAAAACAAGTATGTGAGCCAGAGAATCCGTGCAAGG ACAAGACTCACAACTGCCACAAGCATGCAGAGTGCATCTACCTGGGCCACTTCAGTGACCCCATGTACAA GTGCGAGTGCCAGACTGGCTATGCAGGTGATGGGCTCATCTGCGGGGAGGACTCAGACCTGGACGGTTGG CCCAACAGCAACCTGGTGTGTGCTACCAATGCCACCTACCACTGCGTCAAGGACAACTGCCCCAAACTGC CAAATTCTGGGCAGGAGGATTTTGATAAGGACGGAATCGGAGATGCTTGCGACGAGGACGATGATAACGA CGGCGTGAGCGATGAGAAGGACAATTGCCCACTTCTCTTCAATCCCCGCCAATTAGACTATGATAAGGAT GAGGTTGGAGACCGCTGTGACAATTGCCCCTATGTGCACAACCAAGCACAGATCGACACGGACAACAACG GTGAAGGGGACGCCTGCTCTGTGGATATCGACGGGGATGATGTTTTCAATGAGCGAGACAATTGCCCGTA TGTCTACAACACTGACCAGAGAGACACAGACGGTGACGGCGTTGGGGACCACTGTGACAACTGTCCTCTG ATGCATAACCCAGATCAGATGGATCAGGACAATGATCTCGTTGGAGACCAGTGTGATAACAATGAGGACA TAGATGATGACGGCCACCAGAACAACCAAGACAACTGCCCATACATCTCCAACTCCAACCAGGCTGACCA TGACAATGACGGCAAGGGGGATGCCTGTGACTCCGATGATGACAACGATGGTGTTCCAGATGACAGGGAC AACTGCAGGCTTGTGTTCAACCCCGACCAGGAAGACTCGGATGGCGACGGCCGAGGTGATATTTGTAAAG ATGACTTTGACAATGATAATGTCCCAGATATTGATGACGTGTGCCCCGAGAACAATGCCATCACCGAGAC AGACTTCAGAAACTTTCAGATGGTCCCTCTGGATCCCAAGGGGACCACACAAATTGATCCCAACTGGGTA ATTCGTCACCAAGGCAAAGAGCTGGTGCAGACAGCAAACTCGGACCCTGGCATCGCCGTAGGTTTCGATG AGTTTGGGTCTGTGGACTTCAGCGGCACTTTCTATGTCAACACTGACCGGGACGATGACTACGCCGGCTT CGTCTTTGGCTATCAGTCAAGCAGCCGCTTCTATGTGGTGATGTGGAAGCAGGTGACCCAGACCTACTGG GAAGACAAGCCCAGTCGGGCTTACGGCTACTCTGGTGTGTCACTCAAAGTAGTAAACTCCACGACTGGTA CTGGCGAGCACCTGAGGAATGCCCTGTGGCACACGGGAAACACGGAAGGCCAGGTTCGAACTCTATGGCA TGACCCCAAAAACATTGGCTGGAAGGACTACACCGCCTACAGGTGGCATCTGATTCACAGGCCTAAGACA GGCTACATGAGAGTCTTAGTGCATGAAGGAAAGCAGGTCATGGCTGACTCAGGACCAATTTATGACCAAA CTTATGCTGGTGGACGGCTGGGTCTGTTTGTCTTCTCCCAAGAAATGGTCTACTTCTCAGACCTCAAGTA TGAGTGCAGAGATGCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001169138
Insert Size	3519 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001169138.1, NP_001162609.1
RefSeq Size	5728 bp
RefSeq ORF	3519 bp
Locus ID	292406

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