Sgo2 (NM_001271191) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Sgol2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217517 representing NM_001271191 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTACCCAGGGATAGAAGATGACTCTGTTTCCTCTGGAATTAAGAGACGAGTGAAGGATAGAATTG CAAAGACAAAGTTGAATGTTTCTCTTGCTTCAAAGATCAAAACAAAAATATTAAACAATTCTTCTATTTT CAAGATCTCTCTAAAGCACAACAACAGAGCATTAGCTCGGGCCCTTAGTAAAGAGAAAGAGAATTCTCGA AGAATCACTACCGAAAAGGTGCAATTACAGAAAGAAGTAGAGAAACTGAATTTTGAGAATACCTTTCTTC GCTTAAAACTAAATAATCTGAATAAGAAGCTGATAGAAATAGAATCACAAGTGAGCAATAGTTTGTTAAC TGCAATTGAAATGAGCAGTCTTTCTGAGTTCCATCAAAGTTCTTTTCTCCTGTCAGCTAACAAGAAAAAA AGGGTCAGTAAACAATGCAAGCCTGCACACCTTCCATATGCAAGAGTTCTAATAACTTCAGAAAATGATG ATGATGATGATGATGGTGATGATGATAAATGGAAAACAAAATGTAACAACAGAACTGTATCAAAGACATC ACCTGATAGTACCTCTTCTGTATCAAGACAGTCTTTATCCTTACATCAGTGTAATTTGGAAGTATTCCTT CCTCAAGAAGATAATCAGAAAACATGTGAATCAGGTCATTCAGAATATACTTCAAGTGTTGATGTACTTC CTAATGAGAGCCATTACCACTCAGATCAAAGTCCTAAGAGTTTTCTGAGTAAGATGAAAGATGCTCCGTC TCCCAGCCCCATAAAGGAAAAATTGTCACTTGGTAATGTGACTAAGAGGAAGAAGTGTGTGTCTTCAGCT GCAGACATTCTGCATGTGGCAGATTTAGATCACCAATCAATTTCAAGTCCAGGATCAAATTGGAATAATG AGATAAATGGTTGTACTAATGAAACCAGTAATAACGTGCAAAGAAATACAGAGTGTTTTCCTGACTTACC TTCTGAGTCTTCCAGTGGTCCTAATGTAAAGTGCATGGAACAAGTACAGAAGAACACTGATACCTTTCAC TTGCAGAAAACTGTGTATGAAGACGCTGACATGGAGTTAACTGCTACTGACATAGGCAAGATTGTAACAA TTTCAAAAAGCAATAAAAGTCAAAATAAGAAAAAGGCAGATTGTAGAAAGGAGACTTTCAGAAAAGTGAA AGGTCCAAGCTCTGATAGGAAGAAAGAAAGATCAAAGAGAAAATGTAAAGCTAGTTCGGAAGGTGCTGAG GGAAAGACTGAAAACAGACCAGAGAGAGGCTCTGGTGTCCTAGATGGCAGAGGGGATTCAGAAGATCCAA ACTGTATTTCCAATAGTGAACAGCCATCTCAGGTAAACATGCAGAAGGAAATAACCTTCCAGAAGAGCTC AGATCAGGAGAACGTTCAAAATCAAAGGAGGAGGCAAACGTATACGACAGATGAGCAGGAGGAAACAAAC CCTTTCTCCAGATGTTCAGTCAAATTCCTTCAAGATGGTAAATTTGATCCGTATCAGAACACCCTACACT GTGATTTAAGTAAGCCTTCTCGGCAGACATTTGTGATTCAAACATCAGAAAAAGATAACTTACTGCCAAA CCAAGACAATAAAGAGAACATTTCTGAAAACCTAGAAGATGCAAATGAATTTCATATAGCTCATCTTTCC ATCAAAGATAATGAAAATGTATGTGACCATGAAACTCAAACAATTTTGGACTTGAAAAAGAATGTCAATG CTCAGCAAAATCAAACAAAAATAAATAAGACTAAGCAGAAAATAAATCGAAGAACCAAAATAATTTCTAT TACAAACCAAATAAATGAGGATAATGATAAAGATATTCACGTCCTAGAAAAAGGCAACTTTCCCTTCCAA ACCCAAGCAAATAAAGAAACCACCAGTGGAAACCTAGAAGCTTCAAAAGAATTTGAAACACCTCTTTACA CAAGAGACAGTGGAAGCTTACATGACTGTAACACCCAGAATGTTCTGGATCTGCACAAGCAAATCACTAG TATATACCCTGGTCAGAATAAGTCCCAAATTAGCAAAATCCCTAGACAGAAGCTAAGTCGCAAGACAGAA GTAATTTCTGAAGTGAATTGTTTTAGTAATGACAGAGATGTCCTTTGTTCAGAAAAGGATGAGTCTTTCT TGCTACAAAAGGATAAAGAGATCCCTGGAACCTTGAAAGACGTAAGCGAGTTTGATAAGCCTGCTTTTTA TAACAAAGATAGTGTGAAGTCATGTGATTTTAAGGCTGAACCGTTGTTGGGGTTGAAAAGACATGACCCT AATATGCCACCTGCTTGTCAAGCTGACTCAAAGGTAGATAAGAAGCTTAGACAAAAGGTGAAGCGAAAGA CAGAAATAATTTCTAAAATCAACCAGATACATAAAAATGATGAAGGAAGTGCACATGACCCATTAAATAA GGAGCTCCGTCAGAAGGTTAGTATATCAAAAATCATTTCTGAAATGAACCAAATATATGAGACTATTAAT GAAGATGGGAATGCCTTTAAAAGCTCGCTCAAACATTGCCAAGATGTTATGAGTTGTGACTTTGGGAAAA AAACCAGTAACGAAGAGGAAAATCATGATCTGACTCCAGATCTTTGCTCACTAGTTAAGAAACCAAGGAA AAAGGTATCATGTAAAGCAGGGAACAGTTTGACAGGAGATAAGAACAGGTGTATTTTGCAGTTAACAGGT TCTTCACAGGAGTCCGTCACCTTGGACTCTGGCTTAAAACACGGTCCAAATGAAGCAGATTCTGGCCCTG GAGAGCAGACTAACCTGCCAAAGAAGCAGAAACCAAGCACTGTGAGTACACTGGGAGACGCCTTCTCTCT GAGTGTGGTAAGAGAAGGAAGCCACCCAGCCCAGACAGGTAGTAAAGTGACATCCAAGTCAAAGAAAAGA AAGACCCCTCTAAGATCCTCTGATACCCGTGGAGCAGTGGAGAGAACACCAAACACTGATGGCATTACGG CAGTTTACTTCCAACAGACTGAGAAGGAGAACTATTTGGAGAATGAGAAAATTGCCAAGAGTAAGCCAGA CTTTCGCTCAAAGGTGTTGAAACCTTTATCTGAGATATGTTCATCTAACATAAAGAATTCTTCCTTGGGC AGTATGTGTGAGAGTTCTGTGCCTTTGAGTACTTTTTCTAGTAAACCCCTGATGCTAGAAGAAAGTTCTT CCCTGGACAGTACATGCATCTTTCCAGTAGGTGATGGTGCCCATGAAAAGTTAAAGGAAGGCACTCCTAC ATCCCACCGCAGGACACAGAAGTCAAGACCAGGTAGCAGAACATCCCTGGTCTTGGTGGATAACAGTTCT GTTTCTAGTAACACTGCTAACCCTGAAAATGAGTCAGAAAGGCACTCTTCACACCAGATGAGGAGGAAAA GACAGTGCACCCCTCTCAACCTGACCGAACCAAGCCTTAGAAGCAAGATGAGAAGATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271191
Insert Size	3489 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271191.1, NP_001258120.1
RefSeq Size	3778 bp
RefSeq ORF	3489 bp
Locus ID	316425

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