Cacna2d4 (NM_001191751) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1562038
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217505 representing NM_001191751 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCAGGGACCTGGGTGGCAGCAAGTTCGTTCACAGCAGGAGCACTGTCCTCCTCCCCTGTCCCTCCC CCAGTGGGAACACCATGGCAAGAAGCCCCACATTGTCCTCCAGCCACAGCTGGGGCCACCGTGGACAGCA GACAGCTGCATGGACCTTCCTGCGGAAAATGCCAATTGTCCTGTGGCTGCTGCTCTTGGACACCTCCCTG CCGACTGCAAGGAGCCAGGCTACAATTCCTCTGGAAACAGTGAAGCTCTGGGCTGAAACCTTCGGCAGGG ATCTGTACAGCACGGTGACCAGATACTCGGGTTCTCTCCTGCTGCAGAAGAAGTACAAGGATGCAGAACC CAGTCTGAAGATCAAAGAGGTGGATGGCTTGGAGCTGGTGAAGAAGTTCTCAGAGGACATGGAGACCATG CTTCGGAGGAAAGTTGAGGCTGTGGAGAGCCTGGTGGAGGCCGCTGAGGAGGCTGACCTGAACCATGAGT TCAATGCGTCTCTGGTGTTTAACTACTACAACTCAGTACTGATCAATGAGAAGGATGACAAGGGCAACTA TGTGGAGCTGGGAGCTGAGTTTCTTCTCGAGTCCGACGCCCACTTCAACAACCTGAGGGTGAATGTCTCC ATGAGCAGCGTGCAGCTACCCACCAATGTGTACAACAAAGACCCAGACATTTTAAATGGCGTCTATATGT CCGAAGCCCTGAACCCTGTGTTTGTGGAGAACTTTCAGAGAGACCCCACATTGACCTGGCAGTATTTTGG CAGTTCAACTGGGTTCTTCAGGATCTATCCAGGGATAAAGTGGACACCTGATGAAAATGGAGTCATCGCC TTCGACTGCAGAAACCGTGGCTGGTACATACAAGCCGCCACTTCTCCCAAGGACATCGTCATCCTGGTGG ACATGAGCGGCAGCATGAAGGGGCTGAGGATGGCCATTGCCAAACACACCGTCACAACCATATTGGACAC CCTGGGGGAGAACGACTTCGTGAACATCATCGCGTACAATGACTATGTCCACTACATCGAGCCCTGCTTC AAAGGCATCCTCGTCCAAGCGGATCGAGACAACCGAGAGCATTTCAAGCAGTTGGTGGATGAGCTGATGG TCAAAGGCGTGGGTATCGTGAGTCAAGCTCTAATTGAAGCTTTCCAGATCCTGAAGCAGTTCCAAGAGTC CAGACAAGGAAGCCTCTGCAACCAGGCTATCATGCTTGTCACCGACGGGGCTGTGGAAGACTATGAGCCC GTGTTTGAGACCTATAACTGGCCAGACCGTAAGGTCCGAGTTTTCACCTACCTTATTGGAAGAGAAGTGA CTTTCGCTGACCGCATGAAATGGATCGCCTGCAACAATAAAGGTTACTACACACAGATCTCCACGCTGGC TGATGCGCAGGAGAACGTGATGGAGTACCTGCACGTGCTCAGCCGTCCCATGGTCATCAACCACGACCAC GACATCATCTGGACAGAGGCTTATATGGACAGCCGGCTCCTTACCTCAGAGGCACAAAGCCTGATGCTCC TCACCACAGTGGCCATGCCAGTCTTCAGCAAAAAGAACGAAACACGATCCCATGGCATTCTCCTGGGTGT GGTGGGCTCTGACGTGACCCTAAGAGAGCTCATGAAGCTGGCACCCCGATATAAGCTCGGGGTACATGGC TATGCCTTTTTGAACACTAACAATGGCTACATCCTCTCTCATCCTGACCTCCGACCTTTGTACAGAGAAG GCAAGAAGTTGAAACCCAAGCCCAACTACAACAGTGTGGACCTCTCAGAAGTGGAGTGGGAGGACCAAGC TGAAATCCTGAGGACCGCCATGATCAATGGGGAAACAGGGTCTCACTCCATGGACGTGAAGGTGCCACTG GATAAAGGGAAACGAGTTCTATTCCTGACCAATGACTATTTCTTCACGGACATCAGTGACACGCCTTTCA GCTTGGGAGTGGTGCTCACCAGGGGCCATGGAGAATACATCCTCCTGGGGAACACGTCTGTGGAGGAAGG CCTACATGACTTGCTTCATCCGGATCTGACCCTGGCCAGTGACTGGATCTATTGTATCACGGATATCGAC CCGGACCACCGGAAGCTCAGCCAACTGGAAGCTGTGGTCCGTTTTCTGACAGGGGTGGATCCAGACCTGG AGTGTGATGATGAGGAGCTGGTGCGGGAGGTGCTGTTTGACGCAGTGGTGACCGCACCCATGGAAGCCTA CTGGACAGCACTGGCGCTCAACATCTCCGAAGAGTCAGAGCCTGGTGTGGAAGTGGCCTTTCTGGGGACC CGGGCTGGCCTTCTAAGAAGAAGCTTGTTCGTAGGCTCCGAGAAGGTCTCTGATAGGAAGTTCCTGACAC CTGAAGATGAAGCCAGTATTTTTACCATGGACCACTTCCCACTGTGGTATCGGCAGGCCTCTGAGCAGCC CCCAGGCAGCTTTGTCTTCAATCTCCGCTGGGCAGAGGGACCAGATAGCCCCGGCAAACCAGTGGCGGTA AGGGCCAGCACGGCAATAACTGTGACAGTGGACGGGAAGACGGCCATTGCAGCAGCCGTGGGCATCCAGA TGCAAGCGGACTACCTCCAGCGCCAGTTCTGGGCAGCCATGCAGCAGTGCAACACTGTAGAGGGGCCCTG CCCGAAGCGCTGCCAGGACACTGATCTGGACTGCTTCGTCATAGACAACAATGGCTTCATCCTGATCTCA GAGAGACCCCAAGAGATTGGAAGACTTCTGGGGGAAGCGGATGGTGCTCTCATGACGCAGCTTCTCAGCA TGGGGGTGTTCAGCCGTGTGACCATGTATGACTACCAAGCCATGTGCAAGCCCCCGGATCACCACCACAG TGCAGCCGAATCCCTGATCAGTCCTCTCTCTGCCTTCCTGACGGTGGCCAAGTGGCTACTACATGAATGT CTGCTATTCCTGTTCGAGTGGAGTGCTTGGGGATCTTGGCAGGACAAAGGGTCAGAGGCCAAAGCTGTCT TCCATCACTCCCACAAGCACAAGAAGCAAGACCTCCTGCACCCCTGTGACACAGAGTACCCAGTGTTCGT GCACCAGACGGCCATCCGGGAGGCCAACGGGATCATCGAGTGTGGGGGCTGCCAGAAGACATTCGTGATG CAACAGATTCCCAGCAGCAACCTGCTCCTGCTAGTGACAGACCGTACCTGTGACTGCAGCGCCTACTCTC CCATCCTCCAGGAGGCCACAGAAGTCAAATATAACGCCTCCGTCAAGTGCAACAGGATGCGCTCCCAGAA GCCCCGGAGACGACCAGGCTCCTGCCATGCCTTCCATCCCGAGGAAAATGCCCAGGACTGTGGCGGTGCT TCAGACACTCTGCCTTCATCCCCTCTGCTCCTGCTGGCACTGTGGGCCTGGCGTCTTCCGCCCCAGCTTC TGTGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191751
Insert Size	3438 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191751.1, NP_001178680.1
RefSeq Size	3465 bp
RefSeq ORF	3438 bp
Locus ID	312668

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