Megf10 (NM_001100657) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217504 representing NM_001100657 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTATTTCTTCAAGCTCCTGCCTGGGCCTCATCTGCTCACTGCTGTGCCACTGGCTGAGGGCAGCAT CCTCACTGAACCTGGAAGACCCCAATGTATGCAGCCACTGGGAAAGCTACTCAGTGACTGTGCAGGAGTC TTACCCACATCCCTTCGATCAGATTTACTACACAAGCTGCACTGACATACTGAACTGGTTTAAATGCACT CGGCACAGAGTGAGCTACCGGACAGCCTACCGCCATGGGGAGAAAACCATGTACAGACGCAAATCCCAGT GTTGCCCCGGATTTTATGAAAGTCGAGACGTGTGTGTCCCTCACTGCGCTGATAAATGCGTCCACGGTCG CTGCATCGCTCCAAACACCTGTCAGTGTGAGCCTGGCTGGGGTGGGACCAACTGTAGCAGTGCTTGTGAC GGTGATCACTGGGGACCTCACTGCAGCAGCCGGTGTCAGTGCAAAAACAGAGCTTTGTGTAACCCCATCA CCGGAGCTTGTCACTGCGCCGCGGGCTACCGGGGATGGCGCTGTGAGGACCGTTGTGAACAGGGCACCTA CGGTAACGACTGTCATCAAAGGTGCCAGTGTCAGAATGGAGCGACCTGTGACCACATCACTGGGGAATGT CGTTGTTCACCTGGATACACTGGAGCCTTCTGTGAGGATCTGTGTCCTCCTGGCAAACATGGCCCACAGT GTGAGCAGAGATGTCCCTGCCAAAATGGAGGCGTGTGCCATCATGTCACTGGAGAGTGCTCCTGCCCTTC TGGCTGGATGGGCACAGTGTGTGGTCAGCCCTGCCCCGAGGGTCGCTTTGGAAAGAACTGTTCCCAAGAA TGTCAGTGCCACAATGGAGGAGCGTGTGATGCTGCCACAGGCCAATGTCACTGCAGCCCAGGATACACAG GGGAACGGTGTCAGGATGAATGTCCTGTTGGAACCTATGGAGTTCGCTGTGCTGAGACCTGCAGGTGTGT TAATGGAGGGAAGTGTTACCATGTGAGCGGCACATGCCTATGCGAAGCAGGTTTTTCAGGTGAATTTTGC GAGGCTCGTCTGTGCCCTGAGGGGCTTTACGGCATCAAATGTGACAAGCGGTGCCCCTGCCACCTGGACA ATACTCATAGCTGTCATCCCATGTCTGGAGAGTGTGGCTGCAAGCCGGGTTGGTCTGGACTGTACTGTAA TGAAACATGCTCTCCTGGATTCTACGGGGAAGCTTGCCAACAGATCTGCAGCTGCCAAAACGGGGCTGAC TGTGACAGTGTGAGTGGAAGGTGTGCCTGTGCTCCAGGATTCAAAGGAACTGACTGCTCTACTCCGTGTC CTCTGGGACGCTATGGGATAAATTGTTCTTCTCGCTGTGGCTGTAAAAACGATGCTGTCTGTTCTCCTGT GGATGGATCGTGCATCTGTAAGGCAGGCTGGCACGGGGTGGACTGTTCCATCAGCTGCCCCAGTGGCACA TGGGGCTTTGGCTGTAATCTAACCTGTCAGTGCCTCAATGGCGGTGCCTGCAACACACTGGACGGCACCT GCACCTGTGCGCCGGGATGGCGAGGGGAGAAATGTGAATTTCCCTGCCAGGATGGCACATATGGGCTGAA CTGTGCTGAGCGCTGTGACTGCAGCCATGCAGACGGATGTCACCCCACTACCGGCCATTGCCGATGCCTC CCTGGATGGTCAGGTGTGCACTGTGACAGTGTGTGTGCTGAGGGACGCTGGGGTCCTAACTGCTCGCTGC CCTGCTACTGTAAAAATGGGGCTTCGTGTTCTCCCGATGATGGCATCTGTGAATGCGCACCCGGATTCCG AGGCACCACTTGCCAGAGAATCTGCTCCCCGGGCTTTTATGGACACCGCTGTAGCCAGACATGCCCACAG TGTGTGCACAGCAGTGGACCATGCCACCACATCACAGGCCTGTGTGACTGCTTGCCTGGCTTCACTGGCG CCCTGTGCAATGAAGTGTGTCCCAGTGGCAGATTTGGGAAGAACTGTGCAGGTGTTTGTACCTGCACCAA CAACGGCACCTGTAACCCCATCGACCGGTCCTGTCAGTGCTACCCAGGCTGGATTGGCAGTGACTGCTCC CAGCCCTGTCCACCAGCCCACTGGGGTCCGAACTGCATCCATACATGCAACTGCCACAATGGAGCCTTTT GCAGCGCCTATGATGGGGAATGCAAATGCACTCCTGGCTGGACGGGGCTCTACTGCACTCAGAGATGCCC TCTGGGCTTCTATGGCAAGGACTGTGCACTGGTATGCCAATGTCAAAACGGAGCTGACTGCGACCACATC TCGGGGCAGTGTACCTGCCGCACGGGATTCATGGGACGGCACTGTGAACAGAAATGCCCTGCGGGAACAT ACGGCTATGGCTGTCGCCAGATCTGCGACTGTCTGAACAACTCCACCTGTGACCACATCACCGGCACGTG TTACTGTAGCCCAGGGTGGAAAGGAGCACGATGTGACCAAGCTGGGGTCATCATCGTGGGCAATCTGAAC AGCTTAAGCAGGACCAGCACCGCCCTTCCTGCTGACTCCTATCAGATTGGGGCCATCGCGGGCATCGTGG TCCTTCTGCTTGTTGTGCTCTTCCTGCTGGCGCTGTTCGTTATCTACAGACACAAGCAGAAGAGGAAGGA ATCGAGCATGCCGGCCGTGACCTACACCCCTGCCATGAGGGTTATCAATGCAGACTATACCATTGCAGAA ACCCTGCCTCACAGCAATGGCGGAAATGCCAACAGCCACTACTTCACCAATCCCAGTTATCACACACTTA GCCAGTGTGCCGCGTCCCCTCATGTGAACAACAGGGACAGGATGACCATTGCAAAGTCAAAAAACAATCA ACTGTTTGTGAATCTTAAAAATGTGAATCCAGGGAAGAGAGGCGCTTTGGTGGACTGCACTGGGACGTTG CCAGCTGACTGGAAGCAAGGAGGCTACCTCAATGAACTTGGTGCTTTTGGGCTGGACAGAAGCTACATGG GAAAGTCCTTAAAAGATCTGGGGAAGAATTCTGAGTACAATTCAAGTACTTGTTCCCTAAGCAGCTCGGA GAACCCATATGCCACCATTAAAGACCCTCCTGCGCTCTTGCCTAAAAGCTCCGAGTGTGGCTACGTGGAG ATGAAGTCGCCAGCGCGAAGAGACTCCCCATATGCAGAGATCAATAACTCAACTTCAGCCAACAGGAATG TCTATGAAGTCGAACCTACAGTCAGTGTGGTGCAAGGAGTATTCAGCAACAATGGTCATGTCACCCAAGA CCCATATGACCTTCCAAAGAACAGTCACATCCCTTGCCATTATGACCTGCTGCCAGTAAGGGACAGTTCA TCCTCCCCAAAGAGAGAGGATGGTGGTGGCAGCAGCAGCAGCACCACCACCAGCAACAGCAGCAACAGCA GCGAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001100657
Insert Size	3438 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001100657.1, NP_001094127.1
RefSeq Size	4054 bp
RefSeq ORF	3438 bp
Locus ID	291445
Gene Summary	putative central nervous system protein; transcript recognized by a monoclonal autoantibody which stimulates remyelination of Theiler's virus-demyelinated neurons [RGD, Feb 2006]

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